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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
AGC AKT3 203
TK ABL1 261
AGC ADRBK1 191
AGC AKT1 150
Other AURKA 133
Other AURKB 77
TKL BRAF 456
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK4 6
CMGC CDK5 4
TK EGFR 712
CMGC GSK3A 119
CMGC GSK3B 56
TK JAK2 849
TK JAK3 822
TK LYN 247
CMGC MAPK14 24
CMGC MAPK9 26
CAMK MAPKAPK2 64
AGC PDPK1 82
Other PLK1 53
Other PLK2 82
Other PLK3 62
AGC PRKCZ 252
TK RET 724
Other TTK 525
-FD--YLKLLGKGTFGKVILVREK-----ASGKYYAMKILKKE-V-IIA---KD--EV-AHTLTESRVLKNT-R---HPFLTSLKYSFQ--T------KDRLC--FVMEYVNGG--ELFFHLS----RER-------------------------VFSEDRTRFYGAEIVSALDYLHS-GKIVYRDLKLENLMLD-KDGHI---KITDFGLCKEG----ITDAATMKT---FCGTPEYLAPEVLE-----------DND---YGRAVDWWGLGVVMYEMMC-GRLP---------------FYNQ-------DHEKLFELILM----EDIKFPRTLS---------------------------------------SDAKSLLSGLLIKDPNKRLGGG-PDDAKEIMRHSFF-----------
---ITMKHKLGGGQYGEVYEGVWK-----KYSLTVAVKTLK-E-D-TM----EV--EE-FL-K-EAAVMKEI-K---HPNLVQLLGVCT--R------EPPFY--IITEFMTYG--NLLDYLR-----EC----------------------NRQEVNAVVLLYMATQISSAMEYLEK-KNFIHRDLAARNCLVG-ENHLV---KVADFGLSRLM----T--GDTYTAHAGAKFPIKWTAPESLA-----------YNK---FSIKSDVWAFGVLLWEIATYGMSP---------------YPGI----DLSQVYELLEKDYR----MERPEGCP-----------------------------------------EKVYELMRACWQWNPSDRPS------FAEIHQA--F-----------
--F-SVHRIIGRGGFGEVYGCRKA-----DTGKMYAMKCLDKK-R-IKM---KQ--GE-TLAL-NERIMLSLVSTGDCPFIVCMSYAFH--T------PDKLS--FILDLMNGG--DLHYHLS----QHG-------------------------VFSEADMRFYAAEIILGLEHMHN-RFVVYRDLKPANILLD-EHGHV---RISDLGLAC------DFSKKKPHA---SVGTHGYMAPEVLQ----------KGVA---YDSSADWFSLGCMLFKLLR-GHSP---------------FRQH----KTKDKHEIDRMTLT----MAVELPDSFS---------------------------------------PELRSLLEGLLQRDVNRRLGCL-GRGAQEVKESPFF-----------
--F-EYLKLLGKGTFGKVILVKEK-----ATGRYYAMKILKKE-V-IVA---KD--EV-AHTLTENRVLQNS-R---HPFLTALKYSFQ--T------HDRLC--FVMEYANGG--ELFFHLS----RER-------------------------VFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLD-KDGHI---KITDFGLCKEG----IKDGATMKT---FCGTPEYLAPEVLE-----------DND---YGRAVDWWGLGVVMYEMMC-GRLP---------------FYNQ-------DHEKLFELILM----EEIRFPRTLG---------------------------------------PEAKSLLSGLLKKDPKQRLGGG-SEDAKEIMQHRFF-----------
---FEIGRPLGKGKFGNVYLAREK-----QSKFILALKVLFKA-QLEKA---GV--EH-QLRR-EVEIQSHL-R---HPNILRLYGYFH--D------ATRVY--LILEYAPLG--TVYRELQ----KLS-------------------------KFDEQRTATYITELANALSYCHS-KRVIHRDIKPENLLLG-SAGEL---KIADFGWSV------HAPSSRRTT---LCGTLDYLPPEMIE-----------GRM---HDEKVDLWSLGVLCYEFLV-GKPP---------------FEAN-------TYQETYKRISR----VEFTFPDFVT---------------------------------------EGARDLISRLLKHNPSQRPM------LREVLEHPWI-----------
---FEIGRPLGKGKFGNVYLAREK-----KSHFIVALKVLFKS-Q-IEK---EG--VE-HQLRREIEIQAHL-H---HPNILRLYNYFY--D------RRRIY--LILEYAPRG--ELYKELQ----KSC-------------------------TFDEQRTATIMEELADALMYCHG-KKVIHRDIKPENLLLG-LKGEL---KIADFGWSV------HAPSLRRKT---MCGTLDYLPPEMIE-----------GRM---HNEKVDLWCIGVLCYELLV-GNPP---------------FESA-------SHNETYRRIVK----VDLKFPASVP---------------------------------------MGAQDLISKLLRHNPSERLP------LAQVSAHPWV-----------
---ITVGQRIGSGSFGTVYKGKWH--------GDVAVKMLN-VTA-PT----PQQLQA-FK-N-EVGVLRKT-R---HVNILLFMGYST---------KPQLA--IVTQWCEGS--SLYHHLH-----II-----------------------ETKFEMIKLIDIARQTAQGMDYLHA-KSIIHRDLKSNNIFLH-EDLTV---KIGDFGLATVK--S-R--WSGSHQFEQLSGSILWMAPEVIR-----------MQDKNPYSFQSDVYAFGIVLYELMT-GQLP---------------YSNI-N--NRDQIIFMVGRGYLSPDLSKVRSNCP-----------------------------------------KAMKRLMAECLKKKRDERPL------FPQILA---SI----------
---YTKIEKIGEGTYGVVYKGRHK-----TTGQVVAMKKIRLE-S-EEE---GV--PS-TAIR-EISLLKEL-R---HPNIVSLQDVLM--Q------DSRLY--LIFEFLSM---DLKKYLDS-IPPGQ-------------------------YMDSSLVKSYLYQILQGIVFCHS-RRVLHRDLKPQNLLID-DKGTI---KLADFGLARAF----GIPIRVYTH---EVVTLWYRSPEVLL----------GSAR---YSTPVDIWSIGTIFAELAT-KKPL---------------FHGD----SEIDQLFRIFRALG----TPNNEVWPEVESLQDYKNTFPKWKP-------------GSLASHVKNLDENGLDLLSKMLIYDPAKRIS------GKMALNHPYF-----------
---FQKVEKIGEGTYGVVYKARNK-----LTGEVVALKKIRLD-T-ETE---GV--PS-TAIR-EISLLKEL-N---HPNIVKLLDVIH--T------ENKLY--LVFEFLHQ---DLKKFMDA--SALT-------------------------GIPLPLIKSYLFQLLQGLAFCHS-HRVLHRDLKPQNLLIN-TEGAI---KLADFGLARAF----GVPVRTYTH---EVVTLWYRAPEILL----------GCKY---YSTAVDIWSLGCIFAEMVT-RRAL---------------FPGD----SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR-------------QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS------AKAALAHPFF-----------
--Y-EPVAEIGVGAYGTVYKARDP-----HSGHFVALKSVRVP-N-GGGGGGGL--PI-STVR-EVALLRRL-EAFEHPNVVRLMDVCA--TSR-TDREIKVT--LVFEHVDQ---DLRTYLDK--APPP-------------------------GLPAETIKDLMRQFLRGLDFLHA-NCIVHRDLKPENILVT-SGGTV---KLADFGLARI-----YSYQMALTP---VVVTLWYRAPEVLL-----------QST---YATPVDMWSVGCIFAEMFR-RKPL---------------FCGN----SEADQLGKIFDLIG----LPPEDDWPRDVSLPRGAFPPRGPR---------------PVQSVVPEMEESGAQLLLEMLTFNPHKRIS------AFRALQHSYL-----------
---YEKLEKIGEGTYGTVFKAKNR-----ETHEIVALKRVRLD-D-DDE---GV--PS-SALR-EICLLKEL-K---HKNIVRLHDVLH--S------DKKLT--LVFEFCDQ---DLKKYFD---SCNG-------------------------DLDPEIVKSFLFQLLKGLGFCHS-RNVLHRDLKPQNLLIN-RNGEL---KLADFGLARAF----GIPVRCYSA---EVVTLWYRPPDVLF----------GAKL---YSTSIDMWSAGCIFAELANAGRPL---------------FPGN----DVDDQLKRIFRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT-------------TSLVNVVPKLNATGRDLLQNLLKCNPVQRIS------AEEALQHPYF-----------
--F-KKIKVLGSGAFGTVYKGLWIP-EGEKVKIPVAIKELR-E-A-TS----PKANKE-IL-D-EAYVMASV-D---NPHVCRLLGICL---------TSTVQ--LITQLMPFG--CLLDYVR-----EH-----------------------KDNIGSQYLLNWCVQIAKGMNYLED-RRLVHRDLAARNVLVK-TPQHV---KITDFGLAKLL--G-A--EEKEYHAEGGKVPIKWMALESIL-----------HRI---YTHQSDVWSYGVTVWELMTFGSKP---------------YDGI----PASEISSILEKGER----LPQPPICT-----------------------------------------IDVYMIMVKCWMIDADSRPK------FRELI----IEF---------
---YTDIKVIGNGSFGVVYQARLA-----ETRELVAIKKVLQD-K-RFK---NR----------ELQIMRKL-D---HCNIVRLRYFFY--SSG--EKKDELYLNLVLEYVPETVYRVARHFT---KAKL-------------------------TIPILYVKVYMYQLFRSLAYIHS-QGVCHRDIKPQNLLVDPDTAVL---KLCDFGSAKQ-----LVRGEPNVS---YICSRYYRAPELIF----------GATD---YTSSIDVWSAGCVLAELLL-GQPI---------------FPGD----SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH-------------PWTKVFKSRTPPEAIALCSSLLEYTPSSRLS------PLEACAHSFF-----------
---YTDTKVIGNGSFGVVYQAKLC-----DSGELVAIKKVLQD-K-RFK---NR----------ELQIMRKL-D---HCNIVRLRYFFY--SSG--EKKDEVYLNLVLDYVPETVYRVA---RHYSRAKQ-------------------------TLPVIYVKLYMYQLFRSLAYIHS-FGICHRDIKPQNLLLDPDTAVL---KLCDFGSAKQ-----LVRGEPNVS---YICSRYYRAPELIF----------GATD---YTSSIDVWSAGCVLAELLL-GQPI---------------FPGD----SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH-------------PWTKVFRPRTPPEAIALCSRLLEYTPTARLT------PLEACAHSFF-----------
---LKFLQQLGKGNFGSVEMCRYDP-LQDNTGEVVAVKKLQ-H-S-TE-----EHLRD-FE-R-EIEILKSL-Q---HDNIVKYKGVCYSAG------RRNLK--LIMEYLPYG--SLRDYLQ-----KH-----------------------KERIDHIKLLQYTSQICKGMEYLGT-KRYIHRDLATRNILVE-NENRV---KIGDFGLTKVL-PQ-D--KEYYKVKEPGESPIFWYAPESLT-----------ESK---FSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM----IVFHLIELLKNNGR----LPRPDGCP-----------------------------------------DEIYMIMTECWNNNVNQRPS------FRDLA----LRV---------
--L-KYISQLGKGNFGSVELCRYDP-LGDNTGALVAVKQLQ-H-S-GP----DQ-QRD-FQ-R-EIQILKAL-H---SDFIVKYRGVSYGPG------RQSLR--LVMEYLPSG--CLRDFLQ-----RH-----------------------RARLDASRLLLYSSQICKGMEYLGS-RRCVHRDLAARNILVE-SEAHV---KIADFGLAKLL-PL-D--KDYYVVREPGQSPIFWYAPESLS-----------DNI---FSRQSDVWSFGVVLYELFTYCDKSCSPSAE--FLRMMGCERDV-P--ALCRLLELLEEGQR----LPAPPACP-----------------------------------------AEVHELMKLCWAPSPQDRPS------FSALGP---QL----------
---IKLVKRLGAGQFGEVWMGYYN------NSTKVAVKTLK-P-G-TM----SV--QA-FL-E-EANLMKTL-Q---HDKLVRLYAVVT--R------EEPIY--IITEYMAKG--SLLDFLK-----SD----------------------EGGKVLLPKLIDFSAQIAEGMAYIER-KNYIHRDLRAANVLVS-ESLMC---KIADFGLARVI----E--DNEYTAREGAKFPIKWTAPEAIN-----------FGC---FTIKSDVWSFGILLYEIVTYGKIP---------------YPGR----TNADVMTALSQGYR----MPRVENCP-----------------------------------------DELYDIMKMCWKEKAEERPT------FDYLQSV--L-----------
---YQNLSPVGSGAYGSVCAAFDT-----KTGLRVAVKKLSRP-F-QSI---IH--AK-RTYR-ELRLLKHM-K---HENVIGLLDVFT--PARSLEEFNDVY--LVTHLMGA---DLNNIVK-----CQ-------------------------KLTDDHVQFLIYQILRGLKYIHS-ADIIHRDLKPSNLAVN-EDCEL---KILDFGLAR-------HTDDEMTG---YVATRWYRAPEIML----------NWMH---YNQTVDIWSVGCIMAELLT-GRTL---------------FPGT----DHIDQLKLILRLVG----TPGAELLKKISSESARNYIQSLTQMP-----------KMNFANVFIGANPLAVDLLEKMLVLDSDKRIT------AAQALAHAYF-----------
---YQQLKPIGSGAQGIVCAAFDT-----VLGINVAVKKLSRP-F-QNQ---TH--AK-RAYR-ELVLLKCV-N---HKNIISLLNVFT--PQKTLEEFQDVY--LVMELMDA---NLCQVIH------M-------------------------ELDHERMSYLLYQMLCGIKHLHS-AGIIHRDLKPSNIVVK-SDCTL---KILDFGLART-----ACTNFMMTP---YVVTRYYRAPEVIL-----------GMG---YKENVDIWSVGCIMGELVK-GCVI---------------FQGT----DHIDQWNKVIEQLG----TPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRIS------VDEALRHPYI-----------
KVT---SQVLGLGINGKVLQIFNK-----RTQEKFALKMLQDC-P-KAR-------------R-EVELHWRASQ---CPHIVRIVDVYE--NLY--AGRKCLL--IVMECLDGG--ELFSRIQD--RGDQ-------------------------AFTEREASEIMKSIGEAIQYLHS-INIAHRDVKPENLLYT-SKRPNAILKLTDFGFAKE-----TTSHNSLTT---PCYTPYYVAPEVLG-----------PEK---YDKSCDMWSLGVIMYILLC-GYPP---------------FYSN---HGLAISPGMKTRIRM----GQYEFPNPEWSEVS-----------------------------------EEVKMLIRNLLKTEPTQRMT------ITEFMNHPWI-----------
--F-KFGKILGEGSFSTVVLAREL-----ATSREYAIKILEKR-H-IIK---EN--KVPYVTR-ERDVMSRL-D---HPFFVKLYFTFQ--D------DEKLY--FGLSYAKNG--ELLKYIR----KIG-------------------------SFDETCTRFYTAEIVSALEYLHG-KGIIHRDLKPENILLN-EDMHI---QITDFGTAKVLS--PESKQARANS---FVGTAQYVSPELLT-----------EKS---ACKSSDLWALGCIIYQLVA-GLPP---------------FRAG-------NEYLIFQKIIK----LEYDFPEKFF---------------------------------------PKARDLVEKLLVLDATKRLG------CEEM------EGYGPLKAHPF
-YV--RGRFLGKGGFAKCFEISDA-----DTKEVFAGKIVPKS-L-LLK---PH--QR-EKMSMEISIHRSL-A---HQHVVGFHGFFE--D------NDFVF--VVLELCRRR--SLLELHK----RRK-------------------------ALTEPEARYYLRQIVLGCQYLHR-NRVIHRDLKLGNLFLN-EDLEV---KIGDFGLATKV----EYDGERKKT---LCGTPNYIAPEVLS-----------KKG---HSFEVDVWSIGCIMYTLLV-GKPP---------------FETS-------CLKETYLRIKK----NEYSIPKHIN---------------------------------------PVAASLIQKMLQTDPTARPT------INELLNDEFF-----------
--Y-CRGKVLGKGGFAKCYEMTDL-----TNNKVYAAKIIPHS-R-VAK---PH--QR-EKIDKEIELHRIL-H---HKHVVQFYHYFE--D------KENIY--ILLEYCSRR--SMAHILK----ARK-------------------------VLTEPEVRYYLRQIVSGLKYLHE-QEILHRDLKLGNFFIN-EAMEL---KVGDFGLAARL----EPLEHRRRT---ICGTPNYLSPEVLN-----------KQG---HGCESDIWALGCVMYTMLL-GRPP---------------FETT-------NLKETYRCIRE----ARYTMPSSLL---------------------------------------APAKHLIASMLSKNPEDRPS------LDDIIRHDFF-----------
-YL--KGRLLGKGGFARCYEATDT-----ETGSAYAVKVIPQS-R-VAK---PH--QR-EKILNEIELHRDL-Q---HRHIVRFSHHFE--D------ADNIY--IFLELCSRK--SLAHIWK----ARH-------------------------TLLEPEVRYYLRQILSGLKYLHQ-RGILHRDLKLGNFFIT-ENMEL---KVGDFGLAARL----EPPEQRKKT---ICGTPNYVAPEVLL-----------RQG---HGPEADVWSLGCVMYTLLC-GSPP---------------FETA-------DLKETYRCIKQ----VHYTLPASLS---------------------------------------LPARQLLAAILRASPRDRPS------IDQILRHDFF-----------
--F-DLIRVIGRGSYAKVLLVRLK-----KNDQIYAMKVVKKE-L-VHD---DE--DI-DWVQTEKHVFEQASS---NPFLVGLHSCFQ--T------TSRLF--LVIEYVNGG--DLMFHMQ----RQR-------------------------KLPEEHARFYAAEICIALNFLHE-RGIIYRDLKLDNVLLD-ADGHI---KLTDYGMCKEG----LGPGDTTST---FCGTPNYIAPEILR-----------GEE---YGFSVDWWALGVLMFEMMA-GRSP---------------FDIITDNPDMNTEDYLFQVILE----KPIRIPRFLS---------------------------------------VKASHVLKGFLNKDPKERLGCRPQTGF---------SDIKSHAFF--
--L-VLGKTLGEGEFGKVVKATAFHLKGRAGYTTVAVKMLK-E-N-AS----PSELRD-LL-S-EFNVLKQV-N---HPHVIKLYGACS--Q------DGPLL--LIVEYAKYG--SLRGFLR-----ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAE-MKLVHRDLAARNILVA-EGRKM---KISDFGLSRDV--Y-E--EDSYVKRSQGRIPVKWMAIESLF-----------DHI---YTTQSDVWSFGVLLWEIVTLGGNP---------------YPGI----PPERLFNLLKTGHR----MERPDNCS-----------------------------------------EEMYRLMLQCWKQEPDKRPV------FADISKDL-------------
---YSILKQIGSGGSSKVFQVLNE-----KKQ-IYAIKYVNLE-E-ADN---QT--LD-SYRN-EIAYLNKLQQ--HSDKIIRLYDYEI--T------DQYIY--MVMECGNI---DLNSWLK----KKK-------------------------SIDPWERKSYWKNMLEAVHTIHQ-HGIVHSDLKPANFLIV--DGML---KLIDFGIANQMQ--PDTTSVVKDS---QVGTVNYMPPEAIKDMSSSRENGKSKSK---ISPKSDVWSLGCILYYMTY-GKTP---------------FQQI------INQISKLHAIID----PNHEIEFPDIPE-------------------------------------KDLQDVLKCCLKRDPKQRIS------IPELLAHPYV-----------
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