|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
CAMK CHEK1 203
Other AURKA 133
Other AURKB 77
TKL BRAF 456
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK5 4
CAMK CHEK2 220
CMGC GSK3B 56
CMGC MAPK10 64
CMGC MAPK14 24
CAMK MKNK2 84
AGC PDPK1 82
CAMK PIM1 38
AGC PRKG2 453
AGC ROCK2 92
---W-DLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMK-R-AVDCP--EN-I-KK------EICINKML-NHEN--VVKFYGHRRE------GNIQY--LFLEYCSGG--ELFDRIE----PDI--GMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLD-ERDNL---KISDFGLATVFR------YNNRERLLNK---MCGTLPYVAPELLK----RREF---HAEPVDVWSCGIVLTAMLA-GELPWDQP--------------SDSCQEYSDWKE----KKTYLNPWKKI--D-----------------------------------SAPLALLHKILVENPSA--RIT-----IPDIKKDRW-Y----------
----FEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKA-QLEKAGV--EH-QLRR------EVEIQSHL-RHPN--ILRLYGYFHD------ATRVY--LILEYAPLG--TVYRELQ----KLS--KFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLG-SAGEL---KIADFGWSV----------HAPSSRRTT---LCGTLDYLPPEMIE-----GRM---HDEKVDLWSLGVLCYEFLV-GKPPFEAN---------------TYQETYKRISR----VEFTFPDFVT---------------------------------------EGARDLISRLLKHNPSQ--RPM-----LREVLEHPWI-----------
----FEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKS-Q-IEKEG--VE-HQLR-----REIEIQAHL-HHPN--ILRLYNYFYD------RRRIY--LILEYAPRG--ELYKELQ----KSC--TFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLG-LKGEL---KIADFGWSV----------HAPSLRRKT---MCGTLDYLPPEMIE-----GRM---HNEKVDLWCIGVLCYELLV-GNPPFESA---------------SHNETYRRIVK----VDLKFPASVP---------------------------------------MGAQDLISKLLRHNPSE--RLP-----LAQVSAHPWV-----------
----ITVGQRIGSGSFGTVYKGKWH---GDVAVKMLN-VTA-PT-PQQLQA-FK-N------EVGVLRKT-RHVN--ILLFMGYST-------KPQLA--IVTQWCEGS--SLYHHLH-----IIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLH-EDLTV---KIGDFGLATVK---S----R--WSGSHQFEQLSGSILWMAPEVIR-----MQDKNPYSFQSDVYAFGIVLYELMT-GQLPYSNI-N----------NRDQIIFMVGRGYLSPDLSKVRSNCP-----------------------------------------KAMKRLMAECLKKKRDE--RPL-----FPQILA---SI----------
----YTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLE-S-EEEGV--PS-TAIR------EISLLKEL-RHPN--IVSLQDVLMQ------DSRLY--LIFEFLSM---DLKKYLDS-IPPGQ--YMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLID-DKGTI---KLADFGLARAF--------GIPIRVYTH---EVVTLWYRSPEVLL----GSAR---YSTPVDIWSIGTIFAELAT-KKPLFHGD------------SEIDQLFRIFRALG----TPNNEVWPEVESLQDYKNTFPKWKP-------------GSLASHVKNLDENGLDLLSKMLIYDPAK--RIS-----GKMALNHPYF-----------
----FQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLD-T-ETEGV--PS-TAIR------EISLLKEL-NHPN--IVKLLDVIHT------ENKLY--LVFEFLHQ---DLKKFMDA--SALT--GIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLIN-TEGAI---KLADFGLARAF--------GVPVRTYTH---EVVTLWYRAPEILL----GCKY---YSTAVDIWSLGCIFAEMVT-RRALFPGD------------SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR-------------QDFSKVVPPLDEDGRSLLSQMLHYDPNK--RIS-----AKAALAHPFF-----------
----YEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLD-D-DDEGV--PS-SALR------EICLLKEL-KHKN--IVRLHDVLHS------DKKLT--LVFEFCDQ---DLKKYFD---SCNG--DLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLIN-RNGEL---KLADFGLARAF--------GIPVRCYSA---EVVTLWYRPPDVLF----GAKL---YSTSIDMWSAGCIFAELANAGRPLFPGN------------DVDDQLKRIFRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT-------------TSLVNVVPKLNATGRDLLQNLLKCNPVQ--RIS-----AEEALQHPYF-----------
--YI--MSKTLGSGACGEVKLAFERKTCKKVAIKIISKR-K-FAIGS--AR-EADPALNVETEIEILKKL-NHPC--IIKIKNFF-D------AEDYY--IVLELMEGG--ELFDKVV----GNK--RLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLS-SQEEDCLIKITDFGHSKI---------LGETSLMRT---LCGTPTYLAPEVLVS--VGTAG---YNRAVDCWSLGVILFICLS-GYPPFSEH--------------RTQVSLKDQITS----GKYNFIPEVWAEVS-----------------------------------EKALDLVKKLLVVDPKA--RFT-----TEEALRHPWL-----------
----YTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQD-K-RFKNR---------------ELQIMRKL-DHCN--IVRLRYFFYSSG--EKKDEVYLNLVLDYVPETVYRVA---RHYSRAKQ--TLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVL---KLCDFGSAKQ---------LVRGEPNVS---YICSRYYRAPELIF----GATD---YTSSIDVWSAGCVLAELLL-GQPIFPGD------------SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH-------------PWTKVFRPRTPPEAIALCSRLLEYTPTA--RLT-----PLEACAHSFF-----------
----YQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRP-F-QNQTH--AK-RAYR------ELVLMKCV-NHKN--IISLLNVFTPQKTLEEFQDVY--LVMELMDA---NLCQVIQ------M--ELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVK-SDCTL---KILDFGLART---------AGTSFMMTP---YVVTRYYRAPEVIL-----GMG---YKENVDIWSVGCIMGEMVR-HKILFPGR------------DYIDQWNKVIEQLG----TPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAK--RIS-----VDDALQHPYI-----------
----YQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRP-F-QSIIH--AK-RTYR------ELRLLKHM-KHEN--VIGLLDVFTPARSLEEFNDVY--LVTHLMGA---DLNNIVK-----CQ--KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVN-EDCEL---KILDFGLAR-----------HTDDEMTG---YVATRWYRAPEIML----NWMH---YNQTVDIWSVGCIMAELLT-GRTLFPGT------------DHIDQLKLILRLVG----TPGAELLKKISSESARNYIQSLTQMP-----------KMNFANVFIGANPLAVDLLEKMLVLDSDK--RIT-----AAQALAHAYF-----------
QLQE----DVLGEGAHARVQTCINLITSQEYAVKIIEKQ-P-GHIRS--R---VFR------EVEMLYQCQGHRN--VLELIEFFEE------EDRFY--LVFEKMRGG--SILSHIH----KRR--HFNELEASVVVQDVASALDFLHNKGIAHRDLKPENILCE-HPNQVSPVKICDFDLGSGIKLN-GDCSPISTPELLT---PCGSAEYMAPEVVEAFSEEASI---YDKRCDLWSLGVILYILLS-GYPPFVGRCGSDCGWDRGEACPACQNMLFESIQE----GKYEFPDKDWAHIS-----------------------------------CAAKDLISKLLVRDAKQ--RLS-----AAQVLQHPWV-----------
---F-KFGKILGEGSFSTVVLARELATSREYAIKILEKR-H-IIKEN--KVPYVTR------ERDVMSRL-DHPF--FVKLYFTFQD------DEKLY--FGLSYAKNG--ELLKYIR----KIG--SFDETCTRFYTAEIVSALEYLHGKGIIHRDLKPENILLN-EDMHI---QITDFGTAKVLS------PESKQARANS---FVGTAQYVSPELLT-----EKS---ACKSSDLWALGCIIYQLVA-GLPPFRAG---------------NEYLIFQKIIK----LEYDFPEKFF---------------------------------------PKARDLVEKLLVLDATK--RLG-----CEEM------EGYGPLKAHPF
----YQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKD-R-ISDWG--EL-PNGTRV--PMEVVLLKKV-SSGFSGVIRLLDWFER------PDSFV--LILERPEPV-QDLFDFIT----ERG--ALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGEL---KLIDFGSGA----------LLKDTVYTD---FDGTRVYSPPEWIR----YHRY---HGRSAAVWSLGILLYDMVC-GDIPFEHD---------------------EEIIR----GQVFFRQRVS---------------------------------------SECQHLIRWCLALRPSD--RPT-----FEEIQNHPWM-----------
---L-EIIATLGVGGFGRVELVKVKNENVAFAMKCIRKK-H-IVDTK--QQ-EHVY-----SEKRILEEL-CSPF--IVKLYRTFKD------NKYVY--MLLEACLGG--ELWSILR----DRG--SFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILD-AEGYL---KLVDFGFAKK---------IGSGQKTWT---FCGTPEYVAPEVIL-----NKG---HDFSVDFWSLGILVYELLT-GNPPFSGV---------------DQMMTYNLILK----GIEKMDFPRKIT-------------------------------------RRPEDLIRRLCRQNPTE--RLGNLKNGINDIKKHRWL-----------
----YDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKF-E-MIKRS--DS-AFFW-----EERDIMAFA-NSPW--VVQLFYAFQD------DRYLY--MVMEYMPGG--DLVNLMS-----NY--DVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLD-KHGHL---KLADFGTCMKM-------DETGMVHCDT---AVGTPDYISPEVLKS-QGGDGF---YGRECDWWSVGVFLYEMLV-GDTPFYAD---------------SLVGTYSKIMD----HKNSLCFPEDAEIS-----------------------------------KHAKNLICAFLTDREVRLGRNG-----VEEIRQHPFF-----------
|