|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
CAMK DAPK1 203
TK ABL1 261
Other AURKA 133
CAMK CASK 12
CMGC CDK2 4
CMGC CDK9 2.7.11.23
CAMK CHEK2 220
CMGC CSNK2A1 39
Other EIF2AK2 267
AGC GRK6 186
TK HCK 241
TK LCK 245
CMGC MAPK10 64
CMGC MAPK12 27
CAMK MAPKAPK2 64
Other NEK2 8
CAMK PASK 999
AGC PDPK1 82
CAMK PIM1 38
Other PLK1 53
AGC RPS6KA5 49
AGC SGK1 98
STE STK24 24
Other TTK 525
TK ZAP70 338
----YDTGEELGSGQFAVVKKCRE---K--STGLQYAAKFIKKRR-TKSSR--RG-VSREDI--EREVSIL-KEI-Q-HPN--VITLHEVYEN-----------------------------KTDVILILELVAGG-ELF-DFLA----EKE---SLTEEEATEFLKQILNGVYYLHSL-----QIAHFDLKPENIMLL-DRNVPKPRIKIIDFGLAHK-------IDFGNEFKN---IFGTPEFVAPEIVN-----------YEPLGLEADMWSIGVITYILLS-GA-SPFL-GD----TKQETLANVSAVNYEFEDEYFSNTS-----------------------------------ALAKDFIRRLLVKDPKKRMT-----IQDSLQHPWI-----------
----ITMKHKLGGGQYGEVYEGVW---K--KYSLTVAVKTLK-ED-TM-EV--EE-FL-K------EAAVM-KEI-K-HPN--LVQLLGVCTR-----------------------------EPPFYIITEFMTYG-NLL-DYLR-----ECNRQEVNAVVLLYMATQISSAMEYLEKK-----NFIHRDLAARNCLVG-ENHLV----KVADFGLSRLM------T--GDTYTAHAGAKFPIKWTAPESLA-----------YNKFSIKSDVWAFGVLLWEIATYGM-SPYP-GI-DLSQVYELLEKDYRMERPEGCP-----------------------------------------EKVYELMRACWQWNPSDRPS-----FAEIHQA--F-----------
----FEIGRPLGKGKFGNVYLARE---K--QSKFILALKVLFKAQLEKAGV--EH-QLRR------EVEIQ-SHL-R-HPN--ILRLYGYFHD-----------------------------ATRVYLILEYAPLG-TVY-RELQ----KLS---KFDEQRTATYITELANALSYCHSK-----RVIHRDIKPENLLLG-SAGEL----KIADFGWSV--------HAPSSRRTT---LCGTLDYLPPEMIE-----------GRMHDEKVDLWSLGVLCYEFLV-GK-PPFE-AN----TYQETYKRISRVEFTFPDFVT---------------------------------------EGARDLISRLLKHNPSQRPM-----LREVLEHPWI-----------
----YELCEVIGKGPFSVVRRCIN---R--ETGQQFAVKIVDVAK-FTSSP--GL-STEDL---KREASIC-HML-K-HPH--IVELLETYSS-----------------------------DGMLYMVFEFMDGA-DLCFEIVKR-ADAGF---VYSEAVASHYMRQILEALRYCHDN-----NIIHRDVKPHCVLLA-SKENSA-PVKLGGFGVAIQL------GESGLVAGG---RVGTPHFMAPEVVK-----------REPYGKPVDVWGCGVILFILLS-GC-LPFY-GT-----KERLFEGIIKGKYKMNPRQWSHIS-----------------------------------ESAKDLVRRMLMLDPAERIT-----VYEALNHPWL-----------
----FQKVEKIGEGTYGVVYKARN---K--LTGEVVALKKIRLDT-ETEGV--PS-TAIR------EISLL-KEL-N-HPN--IVKLLDVIHT-----------------------------ENKLYLVFEFLHQ--DLK-KFMDA--SALT---GIPLPLIKSYLFQLLQGLAFCHSH-----RVLHRDLKPQNLLIN-TEGAI----KLADFGLARAF------GVPVRTYTH---EVVTLWYRAPEILL----------GCKYYSTAVDIWSLGCIFAEMVT-RR-ALFP-GD-SEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWAR-------------QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-----AKAALAHPFF-----------
----YEKLAKIGQGTFGEVFKARH---R--KTGQKVALKKVLMEN-EKEGF--PI-TALR------EIKIL-QLL-K-HEN--VVNLIEICRTKASP---------------------YNRCKGSIYLVFDFCEH--DLA-GLLS---NVLV---KFTLSEIKRVMQMLLNGLYYIHRN-----KILHRDMKAANVLIT-RDGVL----KLADFGLARAFSL--AKNSQPNRYTN---RVVTLWYRPPELLL----------GERDYGPPIDLWGAGCIMAEMWT-RS-PIMQ-GN-TEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQK----------RKVKDRLKAYVRDPYALDLIDKLLVLDPAQRID-----SDDALNHDFF-----------
--YI--MSKTLGSGACGEVKLAFE---R--KTCKKVAIKIISKRK-FAIGS--AR-EADPALNVETEIEIL-KKL-N-HPC--IIKIKNFF-D-----------------------------AEDYYIVLELMEGG-ELF-DKVV----GNK---RLKEATCKLYFYQMLLAVQYLHEN-----GIIHRDLKPENVLLS-SQEEDC-LIKITDFGHSKI-------LGETSLMRT---LCGTPTYLAPEVLVS--------VGTAGYNRAVDCWSLGVILFICLS-GY-PPFS-EH---RTQVSLKDQITSGKYNFIPEVWAEVS-----------------------------------EKALDLVKKLLVVDPKARFT-----TEEALRHPWL-----------
----YQLVRKLGRGKYSEVFEAIN---I--TNNEKVVVKILKPVK-KKKIK--R------------EIKIL-ENLRG-GPN--IITLADIVKDP---------------------------VSRTPALVFEHVNNT-DFK-QLY-------Q---TLTDYDIRFYMYEILKALDYCHSM-----GIMHRDVKPHNVMIDHEHRKL----RLIDWGLAEF-------YHPGQEYNV---RVASRYFKGPELLV----------DYQMYDYSLDMWSLGCMLASMIF-RK-EPFFHGH-DNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRK-----RWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLT-----AREAMEHPYF-----------
---F-KEIELIGSGGFGQVFKAKH---R--IDGKTYVIKRVKYNN-EKAER---------------EVKAL-AKL-D-HVN--IVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKG-TLE-QWIEK--RRGE---KLDKVLALELFEQITKGVDYIHSK-----KLIHRDLKPSNIFLV-DTKQV----KIGDFGLVTS-------LKNDGKRTR---SKGTLRYMSPEQIS-----------SQDYGKEVDLYALGLILAELLH-VCDTAFE-T--------SKFFTDLRDGIISDIFDK----------------------------------------KEKTLLQKLLSKKPEDRPN-----TSEILR----TLT--------
---F-RQYRVLGKGGFGEVCACQV---R--ATGKMYACKKLEKKR-IKKRK--GE-AMAL-----NEKQIL-EKV-N-SRF--VVSLAYAYET-----------------------------KDALCLVLTLMNGG-DLK-FHIYH--MGQA---GFPEARAVFYAAEICCGLEDLHRE-----RIVYRDLKPENILLD-DHGHI----RISDLGLAVH-------VPEGQTIKG---RVGTVGYMAPEVVK-----------NERYTFSPDWWALGCLLYEMIA-GQ-SPFQ-QR-KKKIKREEVERLVK-EVPEEYSER--FS-----------------------------------PQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLF-----------
----LKLEKKLGAGQFGEVWMATY---N---KHTKVAVKTMK-PG-SM-SV--EA-FL-A------EANVM-KTL-Q-HDK--LVKLHAVVT------------------------------KEPIYIITEFMAKG-SLL-DFLK-----SDEGSKQPLPKLIDFSAQIAEGMAFIEQR-----NYIHRDLRAANILVS-ASLVC----KIADFGLARVI------E--DNEYTAREGAKFPIKWTAPEAIN-----------FGSFTIKSDVWSFGILLMEIVTYGR-IPYP-GM-SNPEVIRALERGYRMPRPENCP-----------------------------------------EELYNIMMRCWKNRPEERPT-----FEYIQSV--L-----------
----LKLVERLGAGQFGEVWMGYY---N---GHTKVAVKSLK-QG-SM-SP--DA-FL-A------EANLM-KQL-Q-HQR--LVRLYAVVT------------------------------QEPIYIITEYMENG-SLV-DFLK-----TPSGIKLTINKLLDMAAQIAEGMAFIEER-----NYIHRDLRAANILVS-DTLSC----KIADFGLARLI------E--DNEYTAREGAKFPIKWTAPEAIN-----------YGTFTIKSDVWSFGILLTEIVTHGR-IPYP-GM-TNPEVIQNLERGYRMVRPDNCP-----------------------------------------EELYQLMRLCWKERPEDRPT-----FDYLRSV--L-----------
----YQNLKPIGSGAQGIVCAAYD---A--VLDRNVAIKKLSRPF-QNQTH--AK-RAYR------ELVLM-KCV-N-HKN--IISLLNVFTPQKT-----------------------LEEFQDVYLVMELMDA--NLC-QVIQ------M---ELDHERMSYLLYQMLCGIKHLHSA-----GIIHRDLKPSNIVVK-SDCTL----KILDFGLART-------AGTSFMMTP---YVVTRYYRAPEVIL-----------GMGYKENVDIWSVGCIMGEMVR-HK-ILFP-GR-DYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRIS-----VDDALQHPYI-----------
----YRDLQPVGSGAYGAVCSAVD---G--RTGAKVAIKKLYRPF-QSELF--AK-RAYR------ELRLL-KHM-R-HEN--VIGLLDVFTPDET-----------------------LDDFTDFYLVMPFMGT--DLG-KLMK-----HE---KLGEDRIQFLVYQMLKGLRYIHAA-----GIIHRDLKPGNLAVN-EDCEL----KILDFGLAR---------QADSEMTG---YVVTRWYRAPEVIL----------NWMRYTQTVDIWSVGCIMAEMIT-GK-TLFK-GS-DHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELE-----------KKDFASILTNASPLAVNLLEKMLVLDAEQRVT-----AGEALAHPYF-----------
-KVT---SQVLGLGINGKVLQIFN---K--RTQEKFALKMLQDCP-KAR----------R------EVELH-WRASQ-CPH--IVRIVDVYENLY-------------------------AGRKCLLIVMECLDGG-ELF-SRIQD--RGDQ---AFTEREASEIMKSIGEAIQYLHSI-----NIAHRDVKPENLLYT-SKRPNA-ILKLTDFGFAKE-------TTSHNSLTT---PCYTPYYVAPEVLG-----------PEKYDKSCDMWSLGVIMYILLC-GY-PPFY-SNHGLAISPGMKTRIRMGQYEFPNPEWSEVS-----------------------------------EEVKMLIRNLLKTEPTQRMT-----ITEFMNHPWI-----------
----YEVLYTIGTGSYGRCQKIRR---K--SDGKILVWKELDYGS-MTEAE--KQ-MLVS------EVNLL-REL-K-HPN--IVRYYDRIIDR---------------------------TNTTLYIVMEYCEGG-DLA-SVITKGTKERQ---YLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLD-GKQNV----KLGDFGLARIL------NHDTSFAKT---FVGTPYYMSPEQMN-----------RMSYNEKSDIWSLGCLLYELCA-LM-PPFT-AF----SQKELAGKIREGKFRRIPYRY---S-----------------------------------DELNEIITRMLNLKDYHRPS-----VEEILENPL-I----------
----YSTMSPLGSGAFGFVWTAVD---K--EKNKEVVVKFIKKEK-VLEDC--WI-EDPKLGKVTLEIAIL-SRV-E-HAN--IIKVLDIFEN-----------------------------QGFFQLVMEKHGSGLDLF-AFID----RHP---RLDEPLASYIFRQLVSAVGYLRLK-----DIIHRDIKDENIVIA-EDFTI----KLIDFGSAAY-------LERGKLFYT---FCGTIEYCAPEVLM----------GNPYRGPELEMWSLGVTLYTLVF-EE-NPFC-EL------EETVEAAIHPPYLVS-------------------------------------------KELMSLVSGLLQPVPERRTT-----LEKLVTDPWV-----------
---F-KFGKILGEGSFSTVVLARE---L--ATSREYAIKILEKRH-IIKEN--KVPYVTR------ERDVM-SRL-D-HPF--FVKLYFTFQD-----------------------------DEKLYFGLSYAKNG-ELL-KYIR----KIG---SFDETCTRFYTAEIVSALEYLHGK-----GIIHRDLKPENILLN-EDMHI----QITDFGTAKVLS----PESKQARANS---FVGTAQYVSPELLT-----------EKSACKSSDLWALGCIIYQLVA-GL-PPFR-AG----NEYLIFQKIIKLEYDFPEKFF---------------------------------------PKARDLVEKLLVLDATKRLG-----CEEM------EGYGPLKAHPF
----YQVGPLLGSGGFGSVYSGIR---V--SDNLPVAIKHVEKDR-ISDWG--EL-PNGTRV--PMEVVLL-KKV-S-SGFSGVIRLLDWFER-----------------------------PDSFVLILERPEPVQDLF-DFIT----ERG---ALQEELARSFFWQVLEAVRHCHNC-----GVLHRDIKDENILIDLNRGEL----KLIDFGSGA--------LLKDTVYTD---FDGTRVYSPPEWIR----------YHRYHGRSAAVWSLGILLYDMVC-GD-IPFE-HD----------EEIIRGQVFFRQRVS---------------------------------------SECQHLIRWCLALRPSDRPT-----FEEIQNHPWM-----------
--YV--RGRFLGKGGFAKCFEISD---A--DTKEVFAGKIVPKSL-LLKPH--QR-EKMS-----MEISIH-RSL-A-HQH--VVGFHGFFED-----------------------------NDFVFVVLELCRRR-SLL-ELHK----RRK---ALTEPEARYYLRQIVLGCQYLHRN-----RVIHRDLKLGNLFLN-EDLEV----KIGDFGLATKV------EYDGERKKT---LCGTPNYIAPEVLS-----------KKGHSFEVDVWSIGCIMYTLLV-GK-PPFE-TS----CLKETYLRIKKNEYSIPKHIN---------------------------------------PVAASLIQKMLQTDPTARPT-----INELLNDEFF-----------
----FELLKVLGTGAYGKVFLVRKISGH--DTGKLYAMKVLKKAT-IVQKA--KT-TEHTR----TERQVL-EHIRQ-SPF--LVTLHYAFQT-----------------------------ETKLHLILDYINGG-ELF-THLS----QRE---RFTEHEVQIYVGEIVLALEHLHKL-----GIIYRDIKLENILLD-SNGHV----VLTDFGLSKEF-----VADETERAYS---FCGTIEYMAPDIVR---------GGDSGHDKAVDWWSLGVLMYELLT-GA-SPFT-VDGEKNSQAEISRRILKSEPPYPQEMS---------------------------------------ALAKDLIQRLLMKDPKKRLGCGPRDADEIKEHLFF-----------
----FHFLKVIGKGSFGKVLLARH---K--AEEVFYAVKVLQKKA-ILKKK--EE-KHIM-----SERNVLLKNV-K-HPF--LVGLHFSFQT-----------------------------ADKLYFVLDYINGG-ELF-YHLQ----RER---CFLEPRARFYAAEIASALGYLHSL-----NIVYRDLKPENILLD-SQGHI----VLTDFGLCKEN------IEHNSTTST---FCGTPEYLAPEVLH-----------KQPYDRTVDWWCLGAVLYEMLY-GL-PPFY-SR----NTAEMYDNILNKPLQLKPNIT---------------------------------------NSARHLLEGLLQKDRTKRLGAK-DDFMEIKSHV--FF---------
----FTKLEKIGKGSFGEVFKGID---N--RTQKVVAIKIIDLEE-AEDEI--ED-IQ-Q------EITVL-SQC-D-SPY--VTKYYGSYLK-----------------------------DTKLWIIMEYLGGG-SAL-DLLE-----PG---PLDETQIATILREILKGLDYLHSE-----KKIHRDIKAANVLLS-EHGEV----KLADFGVAGQL------TDTQIKRNT---FVGTPFWMAPEVIK-----------QSAYDSKADIWSLGITAIELAR-GE-PPHS-EL-HPMKVLFLIPKNNPPTLEGNYS-----------------------------------------KPLKEFVEACLNKEPSFRPT-----AKELLKHKFI-----------
----YSILKQIGSGGSSKVFQVLN---E--KKQ-IYAIKYVNLEE-ADNQT--LD-SYRN------EIAYL-NKLQQHSDK--IIRLYDYEIT-----------------------------DQYIYMVMECGNI--DLN-SWLK----KKK---SIDPWERKSYWKNMLEAVHTIHQH-----GIVHSDLKPANFLIV--DGML----KLIDFGIANQMQ----PDTTSVVKDS---QVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTY-GK-TPFQ-QI---INQISKLHAIIDPNHEIEFPDIPE-------------------------------------KDLQDVLKCCLKRDPKQRIS-----IPELLAHPYV-----------
LIAD----IELGCGNFGSVRQGVY---RMRKKQIDVAIKVLK-QG-TE-KADTEE-MM-R------EAQIM-HQL-D-NPY--IVRLIGVCQ------------------------------AEALMLVMEMAGGG-PLH-KFLV-----GK-REEIPVSNVAELLHQVSMGMKYLEEK-----NFVHRDLAARNVLLV-NRHYA----KISDFGLSKAL--GA--D--DSYYTARSAGKWPLKWYAPECIN-----------FRKFSSRSDVWSYGVTMWEALSYGQ-KPYK-KM-KGPEVMAFIEQGKRMECPPECP-----------------------------------------PELYALMSDCWIYKWEDRPD-----FLTVEQR--M-----------
|