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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK FGR 203
TK ALK 1116
Other AURKA 133
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK3 4
CMGC CDK5 4
TK EGFR 712
Other EIF2AK2 267
TK FGFR1 478
TK FGFR2 481
TK FYN 271
TKL IRAK4 186
TK JAK2 849
TK LCK 245
CMGC MAPK14 24
TK NTRK3 538
STE PAK4 321
TK PDGFRB 600
AGC PRKCA 339
TKL RAF1 349
TK RET 724
TK SRC 270
TK TNK2 150
TK YES1 277
----ITLERRLGTGCFGDVWLGTWN--------GSTKVAVKTLK-P--G-TM-SP--K-AFL-E-EAQVMKLL--RHDKLVQLYAVVS--------------------------------EEPIYIVTEFMCHGSLLDFLK----NP-------------------------------------------------------------------------------------------------EGQDLRLPQLVDMAAQVAEGMAYMERMNYIHRDLRAANILVGE---RLACKIADFGLARLI----K--DDEY--------------NPCQGSKFPIKWTAPEAAL-FGR---FTIKSDVWSFGILLTELITKGR-IP---------------YPGM-NKREVLEQV-EQGYH----MPCPPGCP------------------------------ASLYEAMEQTWRLDPEERPTFEYLQSFL----------------
---I-TLIRGLGHGAFGEVYEGQVSG--MPNDPSPLQVAVKTLP-E--V-CS-EQDEL-DFL-M-EALIISKF--NHQNIVRCIGVSL--Q-----------------------------SLPRFILLELMAGGDLKSFLR----ETRP--------------------------------------------------------------------------------------------RPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDI--Y-R--ASYY--------------RKGGCAMLPVKWMPPEAFM-EGI---FTSKTDTWSFGVLLWEIFSLGY-MP---------------YPSK-SNQEVLEFV-TSGGR----MDPPKNCP------------------------------GPVYRIMTQCWQHQPEDRPNFAIILER--I--------------
----FEIGRPLGKGKFGNVYLAREK-------QSKFILALKVLFKA--QLEKAGV--E-HQLRR-EVEIQSHL--RHPNILRLYGYFH--D-----------------------------ATRVYLILEYAPLGTVYRELQ---KLS----------------------------------------------------------------------------------------------------KFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGS---AGELKIADFGWSV------HAPSSRR--------------TT---LCGTLDYLPPEMIE-GRM---HDEKVDLWSLGVLCYEFLV-GK-PP---------------FEAN----TYQETY-KRISR----VEFTFPDFVT----------------------------EGARDLISRLLKHNPSQRPMLREVLEHPWI--------------
----YTKIEKIGEGTYGVVYKGRHK-------TTGQVVAMKKIRLE--S-EEEGV--P-STAIR-EISLLKEL--RHPNIVSLQDVLM--Q-----------------------------DSRLYLIFEFLSM-DLKKYLDSIPPGQ----------------------------------------------------------------------------------------------------YMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDD---KGTIKLADFGLARAF----GIPIRVY--------------TH---EVVTLWYRSPEVLLGSAR---YSTPVDIWSIGTIFAELAT-KK-PL---------------FHGD-SEIDQLFRI-FRALG----TPNNEVWPEVESLQDYKNTFPKWKP--GSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYF--------------
----FQKVEKIGEGTYGVVYKARNK-------LTGEVVALKKIRLD--T-ETEGV--P-STAIR-EISLLKEL--NHPNIVKLLDVIH--T-----------------------------ENKLYLVFEFLHQ-DLKKFMDA-SALT----------------------------------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINT---EGAIKLADFGLARAF----GVPVRTY--------------TH---EVVTLWYRAPEILLGCKY---YSTAVDIWSLGCIFAEMVT-RR-AL---------------FPGD-SEIDQLFRI-FRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR--QDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF--------------
----FQKVEKIGEGTYGVVYKAKNR-------ETGQLVALKKIRLD--L-EMEGV--P-STAIR-EISLLKEL--KHPNIVRLLDVVH--N-----------------------------ERKLYLVFEFLSQ-DLKKYMDS-TPGS----------------------------------------------------------------------------------------------------ELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINE---LGAIKLADFGLARAF----GVPLRTY--------------TH---EVVTLWYRAPEILLGSKF---YTTAVDIWSIGCIFAEMVT-RK-AL---------------FPGD-SEIDQLFRI-FRMLG----TPSEDTWPGVTQLPDYKGSFPKWTR--KGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYF--------------
----YEKLEKIGEGTYGTVFKAKNR-------ETHEIVALKRVRLD--D-DDEGV--P-SSALR-EICLLKEL--KHKNIVRLHDVLH--S-----------------------------DKKLTLVFEFCDQ-DLKKYFD--SCNG----------------------------------------------------------------------------------------------------DLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINR---NGELKLADFGLARAF----GIPVRCY--------------SA---EVVTLWYRPPDVLFGAKL---YSTSIDMWSAGCIFAELANAGR-PL---------------FPGN-DVDDQLKRI-FRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT--TSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYF--------------
---F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E--A-TS-PKANK-EIL-D-EAYVMASV--DNPHVCRLLGICL--------------------------------TSTVQLITQLMPFGCLLDYVR----EH--------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKT---PQHVKITDFGLAKLL--G-A--EEKE--------------YHAEGGKVPIKWMALESIL-HRI---YTHQSDVWSYGVTVWELMTFGS-KP---------------YDGI-PASEISSIL-EKGER----LPQPPICT------------------------------IDVYMIMVKCWMIDADSRPKFRELI----IEF------------
---F-KEIELIGSGGFGQVFKAKHR-------IDGKTYVIKRVKYN--N-EKAER----------EVKALAKL--DHVNIVHYNGCWD--GFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEK-RRGE----------------------------------------------------------------------------------------------------KLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVD---TKQVKIGDFGLVTS-----LKNDGKR--------------TR---SKGTLRYMSPEQIS-SQD---YGKEVDLYALGLILAELLH-VCDTA---------------FET--------SKF-FTDLR----DGIISDIFDK-----------------------------KEKTLLQKLLSKKPEDRPNTSEILR----TLT-----------
---L-VLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLK-S--D-AT-EKDLS-DLI-S-EMEMMKMIG-KHKNIINLLGACT--Q-----------------------------DGPLYVIVEYASKGNLREYLQ----ARRPPGLEY-----------------------------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTE---DNVMKIADFGLARDI--H-H--IDYY--------------KKTTNGRLPVKWMAPEALF-DRI---YTHQSDVWSFGVLLWEIFTLGG-SP---------------YPGV-PVEELFKLL-KEGHR----MDKPSNCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----------------
----LTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLK-D--D-AT-EKDLS-DLV-S-EMEMMKMIG-KHKNIINLLGACT--Q-----------------------------DGPLYVIVEYASKGNLREYLR----ARRPPGMEY-----------------------------------------------------------------------------------SYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTE---NNVMKIADFGLARDI--N-N--IDYY--------------KKTTNGRLPVKWMAPEALF-DRV---YTHQSDVWSFGVLMWEIFTLGG-SP---------------YPGI-PVEELFKLL-KEGHR----MDKPANCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----------------
----LQLIKRLGNGQFGEVWMGTWN--------GNTKVAIKTLK-P--G-TM-SP--E-SFL-E-EAQIMKKL--KHDKLVQLYAVVS--------------------------------EEPIYIVTEYMNKGSLLDFLK----DG-------------------------------------------------------------------------------------------------EGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGN---GLICKIADFGLARLI----E--DNEY--------------TARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELVTKGR-VP---------------YPGM-NNREVLEQV-ERGYR----MPCPQDCP------------------------------ISLHELMIHCWKKDPEERPTFEYLQSFL----------------
-VGG----NKMGEGGFGVVYKGYVN-------NTT--VAVKKLAAM--V-DITTE--ELKQQFDQEIKVMAKC--QHENLVELLGFSS--D-----------------------------GDDLCLVYVYMPNGSLLDRLSCLDGTP----------------------------------------------------------------------------------------------------PLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDE---AFTAKISDFGLARASE--KFAQTVMT--------------SR---IVGTTAYMAPEALR--GE---ITPKSDIYSFGVVLLEIIT-GL-PA---------------VDEH-REPQLLLDI-KEEIE----DEEKTIEDYIDKKMNDAD----------------STSVEAMYSVASQCLHEKKNKRPDIKKVQQ----LLQ-----------
----LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-H--S-TE--EHLR-DFE-R-EIEILKSL--QHDNIVKYKGVCYSAG-----------------------------RRNLKLIMEYLPYGSLRDYLQ----KH--------------------------------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVEN---ENRVKIGDFGLTKVL-PQ-D--KEYY--------------KVKEPGESPIFWYAPESLT-ESK---FSVASDVWSFGVVLYELFTYIE-KSKSPPAEFMRMIGNDKQGQM-IVFHLIELL-KNNGR----LPRPDGCP------------------------------DEIYMIMTECWNNNVNQRPSFRDLA----LRV------------
----LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q--G-SM-SP--D-AFL-A-EANLMKQL--QHQRLVRLYAVVT--------------------------------QEPIYIITEYMENGSLVDFLK----TP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSD---TLSCKIADFGLARLI----E--DNEY--------------TAREGAKFPIKWTAPEAIN-YGT---FTIKSDVWSFGILLTEIVTHGR-IP---------------YPGM-TNPEVIQNL-ERGYR----MVRPDNCP------------------------------EELYQLMRLCWKERPEDRPTFDYLRSV--L--------------
----YQNLSPVGSGAYGSVCAAFDT-------KTGLRVAVKKLSRP--F-QSIIH--A-KRTYR-ELRLLKHM--KHENVIGLLDVFT--PARS-----------------------LEEFNDVYLVTHLMGA-DLNNIVK----CQ----------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNE---DCELKILDFGLAR-------HTDDEM--------------TG---YVATRWYRAPEIMLNWMH---YNQTVDIWSVGCIMAELLT-GR-TL---------------FPGT-DHIDQLKLI-LRLVG----TPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYF--------------
---I-VLKRELGEGAFGKVFLAECYN--LSPTKDKMLVAVKALK-D--P-TL-AA-RK-DFQ-R-EAELLTNL--QHEHIVKFYGVCG--D-----------------------------GDPLIMVFEYMKHGDLNKFLR----AHGPDAMIL-----------------------------------------------------------------------------------VDGQPRQAKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVGA---NLLVKIGDFGMSRDV--Y-S--TDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIM-YRK---FTTESDVWSFGVILWEIFTYGK-QP---------------WFQL-SNTEVIECI-TQGRV----LERPRVCP------------------------------KEVYDVMLGCWQREPQQRLNIKEIYKIL----------------
DNFI-----KIGEGSTGIVCIATVR-------SSGKLVAVKKMDLR--K-QQRRE--L---LFN-EVVIMRDY--QHENVVEMYNSYL--V-----------------------------GDELWVVMEFLEGGALTDIVT----HT----------------------------------------------------------------------------------------------------RMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTH---DGRVKLSDFGFCAQV----SKEVPRR--------------KS---LVGTPYWMAPELIS-RLP---YGPEVDIWSLGIMVIEMVD-GE-PP---------------YFNE-PPLKAMKMI-RDNLP----PRLKNLHKVS----------------------------PSLKGFLDRLLVRDPAQRATAAELLKHPFL--------------
---L-VLGRTLGSGAFGQVVEATAHG--LSHSQATMKVAVKMLK-S--T-AR-SSEKQ-ALM-S-ELKIMSHLG-PHLNVVNLLGACT--K-----------------------------GGPIYIITEYCRYGDLVDYLH----RNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICE---GKLVKICDFGLARDI--M-R--DSNY--------------ISKGSTFLPLKWMAPESIF-NSL---YTTLSDVWSFGILLWEIFTLGG-TP---------------YPELPMNEQFYNAI-KRGYR----MAQPAHAS------------------------------DEIYEIMQKCWEEKFEIRPPFSQLVL---LL-------------
---F-NFLMVLGKGSFGKVMLADRK-------GTEELYAIKILKKD--V-VIQDD--D-VECTMVEKRVLALL-DKPPFLTQLHSCFQ--T-----------------------------VDRLYFVMEYVNGGDLMYHIQ---QVG----------------------------------------------------------------------------------------------------KFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDS---EGHIKIADFGMCKEH----MMDGVTT--------------RT---FCGTPDYIAPEIIA-YQP---YGKSVDWWAYGVLLYEMLA-GQ-PP---------------FDGE----DEDELF-QSIME----HNVSYPKSLS----------------------------KEAVSVCKGLMTKHPAKRLGC---------GPEGERDVREHAFF
--VM--LSTRIGSGSFGTVYKGKWH----------GDVAVKILK-V-VD-PT-PEQFQ-AFR-N-EVAVLRKT--RHVNILLFMGYMT--------------------------------KDNLAIVTQWCEGSSLYKHLH----VQ--------------------------------------------------------------------------------------------------ETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE---GLTVKIGDFGLATVK--S-R--WSGS--------------QQVEQPTGSVLWMAPEVIR-MQDNNPFSFQSDVYSYGIVLYELMT-GE-LP---------------YSHINNRDQIIFMV-GRGYASPDLSKLYKNCP------------------------------KAMKRLVADCVKKVKEERPLFPQILSS--I--------------
---L-VLGKTLGEGEFGKVVKATAFH--LKGRAGYTTVAVKMLK-E--N-AS-PSELR-DLL-S-EFNVLKQV--NHPHVIKLYGACS--Q-----------------------------DGPLLLIVEYAKYGSLRGFLR----ESRKVGPGYLGSG---------------------------------------------------------------------------GSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAE---GRKMKISDFGLSRDV--Y-E--EDSY--------------VKRSQGRIPVKWMAIESLF-DHI---YTTQSDVWSFGVLLWEIVTLGG-NP---------------YPGI-PPERLFNLL-KTGHR----MERPDNCS------------------------------EEMYRLMLQCWKQEPDKRPVFADISKDL----------------
---L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P--G-TM-SP--E-AFL-Q-EAQVMKKL--RHEKLVQLYAVVS--------------------------------EEPIYIVTEYMSKGSLLDFLK----GE-------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGE---NLVCKVADFGLARLI----E--DNEY--------------TARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELTTKGR-VP---------------YPGM-VNREVLDQV-ERGYR----MPCPPECP------------------------------ESLHDLMCQCWRKEPEERPTFEYLQAF--L--------------
---L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVL-SQ-PEAMD-DFI-R-EVNAMHSL--DHRNLIRLYGVVL--------------------------------TPPMKMVTELAPLGSLLDRLR----KH--------------------------------------------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLAT---RDLVKIGDFGLMRAL-PQ-N--DDHY--------------VMQEHRKVPFAWCAPESLK-TRT---FSHASDTWMFGVTLWEMFTYGQ-EP---------------WIGL-NGSQILHKIDKEGER----LPRPEDCP------------------------------QDIYNVMVQCWAHKPEDRPTFVALRDF--L--------------
---L-RLEVKLGQGCFGEVWMGTWN--------GTTKVAIKTLK-P--G-TM-MP--E-AFL-Q-EAQIMKKL--RHDKLVPLYAVVS--------------------------------EEPIYIVTEFMSKGSLLDFLK----EG-------------------------------------------------------------------------------------------------DGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGE---NLVCKIADFGLARLI----E--DNEY--------------TARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILQTELVTKGR-VP---------------YPGM-VNREVLEQV-ERGYR----MPCPQGCP------------------------------ESLHELMNLCWKKDPDERPTFEYIQSFL----------------
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