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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
AGC GRK7 203
CAMK BRSK2 19
CAMK CAMK2A 13
CAMK CAMK2B 14
CAMK CAMK2D 14
CMGC CDK2 4
CMGC CSNK2A1 39
CMGC CSNK2A2 40
CAMK DAPK1 13
CAMK DAPK2 23
CAMK DAPK3 13
AGC GRK4 187
TKL MAP3K9 144
CAMK MGC42105 74
Other NEK1 4
Other NEK2 8
Other NEK5 4
Other NEK9 52
CAMK NUAK2 97
CAMK PIM1 38
CAMK PIM3 40
AGC PKN2 657
Other PLK1 53
Other PLK2 82
Other PLK3 62
AGC PRKCD 349
CAMK PRKD3 576
AGC ROCK1 76
AGC RPS6KA2 59
AGC RPS6KA3 68
AGC RPS6KA4 33
AGC RPS6KA6 73
STE SLK 34
STE STK10 36
--F-TEFRVLGKGGFGEVCAVQVKNTGK---MYACKKLDKK-RLKKKGGEKMAL---LEKEILEKV-S----SPF--IVSLAYAFES--KTHLCLVMSLMNGG-DLKFHIYN--VGTRGLDMSRVIFYSAQIACGMLHLHE-L-----GIVYRDMKPENVLLD-DLGNC--------RLSDLGLAVE--MKGGKPITQRAGTNGYMAPEILM----EKVSYSYPVDWFAMGCSIYEMVAGRTPFK-DY---KEKVSKEDLKQRTLQDEVKFQHDNF---T------------------------------EEAKDICRLFLAKKPEQ--RLG-----SRE-------KSDDPR
---YRLEKTLGKGQTGLVKLGVHCVTCQ---KVAIKIVNRE-KLSESVLMKVER----EIAILKLI-E----HPH--VLKLHDVYEN--KKYLYLVLEHVSGG-ELFDYLV----KKGRLTPKEARKFFRQIISALDFCHS-H-----SICHRDLKPENLLLD-EKNNI--------RIADFGMASL--QVGDSLLETSCGSPHYACPEVIR----GEKYDGRKADVWSCGVILFALLVGALPFD-DD-------NLRQLLEKVKRGVFHMPHFIP----------------------------------PDCQSLLRGMIEVDAAR--RLT-----LEHIQKHIW-Y-----
---YQLFEELGKGAFSVVRRCVKVLAGQ---EYAAKIINTK-KLSARDHQKLER----EARICRLL-K----HPN--IVRLHDSISE--EGHHYLIFDLVTGG-ELFEDIV----AREYYSEADASHCIQQILEAVLHCHQ-M-----GVVHRDLKPENLLLA-SKLKGA-----AVKLADFGLAIEV-EGEQQAWFGFAGTPGYLSPEVLR-----KDPYGKPVDLWACGVILYILLVGYPPFW-DE-------DQHRLYQQIKAGAYDFPSPEWDTVT------------------------------PEAKDLINKMLTINPSK--RIT-----AAEALKHPWI------
---YQLYEDIGKGAFSVVRRCVKLCTGH---EYAAKIINTK-KLSARDHQKLER----EARICRLL-K----HSN--IVRLHDSISE--EGFHYLVFDLVTGG-ELFEDIV----AREYYSEADASHCIQQILEAVLHCHQ-M-----GVVHRDLKPENLLLA-SKCKGA-----AVKLADFGLAIEV-QGDQQAWFGFAGTPGYLSPEVLR-----KEAYGKPVDIWACGVILYILLVGYPPFW-DE-------DQHKLYQQIKAGAYDFPSPEWDTVT------------------------------PEAKNLINQMLTINPAK--RIT-----AHEALKHPWV------
---YQLFEELGKGAFSVVRRCMKIPTGQ---EYAAKIINTK-KLSARDHQKLER----EARICRLL-K----HPN--IVRLHDSISE--EGFHYLVFDLVTGG-ELFEDIV----AREYYSEADASHCIQQILESVNHCHL-N-----GIVHRDLKPENLLLA-SKSKGA-----AVKLADFGLAIEV-QGDQQAWFGFAGTPGYLSPEVLR-----KDPYGKPVDMWACGVILYILLVGYPPFW-DE-------DQHRLYQQIKAGAYDFPSPEWDTVT------------------------------PEAKDLINKMLTINPAK--RIT-----ASEALKHPWI------
---FQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIRLD-TETEGVPSTAIR----EISLLKEL-N----HPN--IVKLLDVIHT--ENKLYLVFEFLHQ--DLKKFMDA--SALTGIPLPLIKSYLFQLLQGLAFCHS-H-----RVLHRDLKPQNLLIN-TEGAI--------KLADFGLARAF-GVPVRTYTHEVVTLWYRAPEILL----GCKYYSTAVDIWSLGCIFAEMVTRRALFP-GD----SEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWAR--------QDFSKVVPPLDEDGRSLLSQMLHYDPNK--RIS-----AKAALAHPFF------
---YQLVRKLGRGKYSEVFEAINITNNE---KVVVKILKPV-KKKKIKR---------EIKILENLRG----GPN--IITLADIVKDPVSRTPALVFEHVNNT-DFKQLY-------QTLTDYDIRFYMYEILKALDYCHS-M-----GIMHRDVKPHNVMIDHEHRKL--------RLIDWGLAEF--YHPGQEYNVRVASRYFKGPELLV----DYQMYDYSLDMWSLGCMLASMIFRKEPFFHGH----DNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQS--RLT-----AREAMEHPYF------
---YQLVRKLGRGKYSEVFEAINITNNE---RVVVKILKPV-KKKKIKR---------EVKILENLRG----GTN--IIKLIDTVKDPVSKTPALVFEYINNT-DFKQLY-------QILTDFDIRFYMYELLKALDYCHS-K-----GIMHRDVKPHNVMIDHQQKKL--------RLIDWGLAEF--YHPAQEYNVRVASRYFKGPELLV----DYQMYDYSLDMWSLGCMLASMIFRREPFFHGQ----DNYDQLVRIAKVLGTEELYGYLKKYHIDLDPHFNDILGQHSRKRWENFIHSENRHLVSPEALDLLDKLLRYDHQQ--RLT-----AKEAMEHPYF------
---YDTGEELGSGQFAVVKKCREKSTGL---QYAAKFIKKR-RTKSSRRGVSREDIEREVSILKEI-Q----HPN--VITLHEVYEN--KTDVILILELVAGG-ELFDFLA----EKESLTEEEATEFLKQILNGVYYLHS-L-----QIAHFDLKPENIMLL-DRNVPKP----RIKIIDFGLAHK--IDFGNEFKNIFGTPEFVAPEIVN-----YEPLGLEADMWSIGVITYILLSGASPFL-GD-------TKQETLANVSAVNYEFEDEYFSNTS------------------------------ALAKDFIRRLLVKDPKK--RMT-----IQDSLQHPWI------
---YDIGEELGSGQFAIVKKCREKSTGL---EYAAKFIKKR-QSRASRRGVSREEIEREVSILRQV-L----HHN--VITLHDVYEN--RTDVVLILELVSGG-ELFDFLA----QKESLSEEEATSFIKQILDGVNYLHT-K-----KIAHFDLKPENIMLL-DKNIPIP----HIKLIDFGLAHE--IEDGVEFKNIFGTPEFVAPEIVN-----YEPLGLEADMWSIGVITYILLSGASPFL-GD-------TKQETLANITAVSYDFDEEFFSQTS------------------------------ELAKDFIRKLLVKETRK--RLT-----IQEALRHPWI------
---YEMGEELGSGQFAIVRKCRQKGTGK---EYAAKFIKKR-RLSSSRRGVSREEIEREVNILREI-R----HPN--IITLHDIFEN--KTDVVLILELVSGG-ELFDFLA----EKESLTEDEATQFLKQILDGVHYLHS-K-----RIAHFDLKPENIMLL-DKNVPNP----RIKLIDFGIAHK--IEAGNEFKNIFGTPEFVAPEIVN-----YEPLGLEADMWSIGVITYILLSGASPFL-GE-------TKQETLTNISAVNYDFDEEYFSNTS------------------------------ELAKDFIRRLLVKDPKR--RMT-----IAQSLEHSWI------
-FR--HYRVLGKGGFGEVCACQVRATGK---MYACKKLQKK-RIKKRKGEAMAL---NEKRILEKV-Q----SRF--VVSLAYAYET--KDALCLVLTIMNGG-DLKFHIYN--LGNPGFDEQRAVFYAAELCCGLEDLQR-E-----RIVYRDLKPENILLD-DRGHI--------RISDLGLATE--IPEGQRVRGRVGTVGYMAPEVVN-----NEKYTFSPDWWGLGCLIYEMIQGHSPFK-KY----KEKVKWEEVDQRIKNDTEEYSE---KFS------------------------------EDAKSICRMLLTKNPSK--RLGCRGEGAAGVKQHPV-F-----
--L-TLEEIIGIGGFGKVYRAFWIGDE-----VAVKAARHDPDEDISQTIENVR---QEAKLFAML-K----HPN--IIALRGVCLK--EPNLCLVMEFARGG-PLNRVLS-----GKRIPPDILVNWAVQIARGMNYLHD-EA--IVPIIHRDLKSSNILIL-QKVENGDLSNKILKITDFGLAR---EWHRTTKMSAAGTYAWMAPEVIR-----ASMFSKGSDVWSYGVLLWELLTGEVPFR-GI-------DGLAVAYGVAMNKLALPIPSTCP--------------------------------EPFAKLMEDCWNPDPHS--RPS-----FTNILDQ---LT----
---YRIRGEIGSGNFSQVKLGIHSLTKE---KVAIKILDKT-KLDQKTQRLLSR----EISSMEKL-H----HPN--IIRLYEVVET--LSKLHLVMEYAGGG-ELFGKIS----TEGKLSEPESKLIFSQIVSAVKHMHE-N-----QIIHRDLKAENVFYT-SNTCV--------KVGDFGFSTV--SKKGEMLNTFCGSPPYAAPELFR----DEHYIGIYVDIWALGVLLYFMVTGTMPFR-AE-------TVAKLKKSILEGTYSVPPHV----S------------------------------EPCHRLIRGVLQQIPTE--RYG-----IDCIMNDEWM------
--Y-VRLQKIGEGSFGKAILVKSTEDGR---QYVIKEINIS-RMSSKEREESRR----EVAVLANM-K----HPN--IVQYRESFEE--NGSLYIVMDYCEGG-DLFKRINA--QKGVLFQEDQILDWFVQICLALKHVHD-R-----KILHRDIKSQNIFLT-KDGTV--------QLGDFGIARVL-NSTVELARTCIGTPYYLSPEICE-----NKPYNNKSDIWALGCVLYELCTLKHAFE-AG-------SMKNLVLKIISGSFPPVSLHYS---------------------------------YDLRSLVSQLFKRNPRD--RPS-----VNSILEKGF-I-----
---YEVLYTIGTGSYGRCQKIRRKSDGK---ILVWKELDYG-SMTEAEKQMLVS----EVNLLREL-K----HPN--IVRYYDRIIDRTNTTLYIVMEYCEGG-DLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHR-RSDGGHTVLHRDLKPANVFLD-GKQNV--------KLGDFGLARIL-NHDTSFAKTFVGTPYYMSPEQMN-----RMSYNEKSDIWSLGCLLYELCALMPPFT-AF-------SQKELAGKIREGKFRRIPYRY---S------------------------------DELNEIITRMLNLKDYH--RPS-----VEEILENPL-I-----
---YDVIKAIGQGAFGKAYLAKGKSDSK---HCVIKEINFE-KMPIQEKEASKK----EVILLEKM-K----HPN--IVAFFNSFQE--NGRLFIVMEYCDGG-DLMKRINR--QRGVLFSEDQILGWFVQISLGLKHIHD-R-----KILHRDIKAQNIFLS-KNGMV-------AKLGDFGIARVL-NNSMELARTCIGTPYYLSPEICQ-----NKPYNNKTDIWSLGCVLYELCTLKHPFE-GN-------NLQQLVLKICQAHFAPISPGF---S------------------------------RELHSLISQLFQVSPRD--RPS-----INSILKRPFL------
YIP---IRVLGRGAFGEATLYRRTEDDS---LVVWKEVDLT-RLSEKERRDALN----EIVILALL-Q----HDN--IIAYYNHFMD--NTTLLIELEYCNGG-NLYDKILR--QKDKLFEEEMVVWYLFQIVSAVSCIHK-A-----GILHRDIKTLNIFLT-KANLI--------KLGDYGLAKKL-NSEYSMAETLVGTPYYMSPELCQ-----GVKYNFKSDIWAVGCVIFELLTLKRTFD-AT-------NPLNLCVKIVQGIRAMEVDSS-QYS------------------------------LELIQMVHSCLDQDPEQ--RPT-----ADELLDRPL-L-----
---YEFLETLGKGTYGKVKKARESSGR----LVAIKSIRKD-KIKDEQDLMHIR---REIEIMSSL-N----HPH--IIAIHEVFEN--SSKIVIVMEYASRG-DLYDYIS----ERQQLSEREARHFFRQIVSAVHYCHQ-N-----RVVHRDLKLENILLD-ANGNI--------KIADFGLSNL--YHQGKFLQTFCGSPLYASPEIVN----GKPYTGPEVDSWSLGVLLYILVHGTMPFD-GH-------DHKILVKQISNGAYREPPK-----P------------------------------SDACGLIRWLLMVNPTR--RAT-----LEDVASHWW-V-----
---YQVGPLLGSGGFGSVYSGIRVSDNL---PVAIKHVEKD-RISDWGELPNGTRVPMEVVLLKKV-S----SGFSGVIRLLDWFER--PDSFVLILERPEPVQDLFDFIT----ERGALQEELARSFFWQVLEAVRHCHN-C-----GVLHRDIKDENILIDLNRGEL--------KLIDFGSGA---LLKDTVYTDFDGTRVYSPPEWIR----YHRYHGRSAAVWSLGILLYDMVCGDIPFE-HD-------------EEIIRGQVFFRQRVS----------------------------------SECQHLIRWCLALRPSD--RPT-----FEEIQNHPWM------
---YQVGAVLGSGGFGTVYAGSRIADGL---PVAVKHVVKE-RVTEWGSLGGATV-PLEVVLLRKV-GAAGGARG--VIRLLDWFER--PDGFLLVLERPEPAQDLFDFIT----ERGALDEPLARRFFAQVLAAVRHCHS-C-----GVVHRDIKDENLLVDLRSGEL--------KLIDFGSGA---LLKDTVYTDFDGTRVYSPPEWIR----YHRYHGRSATVWSLGVLLYDMVCGDIPFE-QD-------------EEILRGRLLFRRRVS----------------------------------PECQQLIRWCLSLRPSE--RPS-----LDQIAAHPWM------
-FR--CCAVLGRGHFGKVLLAEYKNTNE---MFAIKALKKG-DIVARDEVDSLM---CEKRIFETV-NS-VRHPF--LVNLFACFQT--KEHVCFVMEYAAGG-DLMMHIH-----TDVFSEPRAVFYAACVVLGLQYLHE-H-----KIVYRDLKLDNLLLD-TEGFV--------KIADFGLCKEG-MGYGDRTSTFCGTPEFLAPEVLT-----ETSYTRAVDWWGLGVLIYEMLVGESPFP-GD-------DEEEVFDSIVNDEVRYPRFLS----------------------------------TEAISIMRRLLRRNPER--RLGASEKDAEDVKKHPFF------
-YV--RGRFLGKGGFAKCFEISDADTKE---VFAGKIVPKS-LLLKPHQREKMS---MEISIHRSL-A----HQH--VVGFHGFFED--NDFVFVVLELCRRR-SLLELHK----RRKALTEPEARYYLRQIVLGCQYLHR-N-----RVIHRDLKLGNLFLN-EDLEV--------KIGDFGLATKV-EYDGERKKTLCGTPNYIAPEVLS-----KKGHSFEVDVWSIGCIMYTLLVGKPPFE-TS-------CLKETYLRIKKNEYSIPKHIN----------------------------------PVAASLIQKMLQTDPTA--RPT-----INELLNDEFF------
--Y-CRGKVLGKGGFAKCYEMTDLTNNK---VYAAKIIPHS-RVAKPHQREKID---KEIELHRIL-H----HKH--VVQFYHYFED--KENIYILLEYCSRR-SMAHILK----ARKVLTEPEVRYYLRQIVSGLKYLHE-Q-----EILHRDLKLGNFFIN-EAMEL--------KVGDFGLAARL-EPLEHRRRTICGTPNYLSPEVLN-----KQGHGCESDIWALGCVMYTMLLGRPPFE-TT-------NLKETYRCIREARYTMPSSLL----------------------------------APAKHLIASMLSKNPED--RPS-----LDDIIRHDFF------
-YL--KGRLLGKGGFARCYEATDTETGS---AYAVKVIPQS-RVAKPHQREKIL---NEIELHRDL-Q----HRH--IVRFSHHFED--ADNIYIFLELCSRK-SLAHIWK----ARHTLLEPEVRYYLRQILSGLKYLHQ-R-----GILHRDLKLGNFFIT-ENMEL--------KVGDFGLAARL-EPPEQRKKTICGTPNYVAPEVLL-----RQGHGPEADVWSLGCVMYTLLCGSPPFE-TA-------DLKETYRCIKQVHYTLPASLS----------------------------------LPARQLLAAILRASPRD--RPS-----IDQILRHDFF------
-FI--FHKVLGKGSFGKVLLGELKGRGE---YFAIKALKKD-VVLIDDDVECTM---VEKRVLTLAAE----NPF--LTHLICTFQT--KDHLFFVMEFLNGG-DLMYHIQ----DKGRFELYRATFYAAEIMCGLQFLHS-K-----GIIYRDLKLDNVLLD-RDGHI--------KIADFGMCKEN-IFGESRASTFCGTPDYIAPEILQ-----GLKYTFSVDWWSFGVLLYEMLIGQSPFH-GD-------DEDELFESIRVDTPHYPRWIT----------------------------------KESKDILEKLFEREPTK--RLG----VTGNIKIHPFF------
FAD----EVLGSGQFGIVYGGKHRKTGR---DVAIKVIDKM-RFPTKQESQLRN----EVAILQNL-H----HPG--IVNLECMFET--PERVFVVMEKLHGD-MLEMILS---SEKSRLPERITKFMVTQILVALRNLHF-K-----NIVHCDLKPENVLLA-SAEPFP-----QVKLCDFGFARI--IGEKSFRRSVVGTPAYLAPEVLR-----SKGYNRSLDMWSVGVIIYVSLSGTFPFN-ED---------EDINDQIQNAAFMYPPNPWREIS------------------------------GEAIDLINNLLQVKMRK--RYS-----VDKSLSHPWL------
---YEVVKVIGRGAFGEVQLVRHKSTRK---VYAMKLLSKF-EMIKRSDSAFFW---EERDIMAFA-N----SPW--VVQLFYAFQD--DRYLYMVMEYMPGG-DLVNLMS-----NYDVPEKWARFYTAEVVLALDAIHS-M-----GFIHRDVKPDNMLLD-KSGHL--------KLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKS-QGGDGYYGRECDWWSVGVFLYEMLVGDTPFY-AD-------SLVGTYSKIMNHKNSLTFPDDNDIS------------------------------KEAKNLICAFLTDREVRLGRNG-----VEEIKRHLFF------
---FELLKVLGQGSYGKVFLVRKVKGSDAGQLYAMKVLKKA-TLKVRDRVRSKM----ERDILAEV-N----HPF--IVKLHYAFQT--EGKLYLILDFLRGG-DLFTRLS----KEVMFTEEDVKFYLAELALALDHLHS-L-----GIIYRDLKPENILLD-EEGHI--------KITDFGLSKEA-IDHDKRAYSFCGTIEYMAPEVVN-----RRGHTQSADWWSFGVLMFEMLTGSLPFQ-GK-------DRKETMALILKAKLGMPQFLS----------------------------------GEAQSLLRALFKRNPCN--RLGAGIDGVEEIKRHPFF------
---FELLKVLGQGSFGKVFLVKKISGSDARQLYAMKVLKKA-TLKVRDRVRTKM----ERDILVEV-N----HPF--IVKLHYAFQT--EGKLYLILDFLRGG-DLFTRLS----KEVMFTEEDVKFYLAELALALDHLHS-L-----GIIYRDLKPENILLD-EEGHI--------KLTDFGLSKES-IDHEKKAYSFCGTVEYMAPEVVN-----RRGHTQSADWWSFGVLMFEMLTGTLPFQ-GK-------DRKETMTMILKAKLGMPQFLS----------------------------------PEAQSLLRMLFKRNPAN--RLGAGPDGVEEIKRHSFF------
REP-----ALGQGSFSVCRRCRQRQSGQ---EFAVKILSRR-LEANTQR---------EVAALRLCQS----HPN--VVNLHEVHHD--QLHTYLVLELLRGG-ELLEHIR----KKRHFSESEASQILRSLVSAVSFMHEEA-----GVVHRDLKPENILYA-DDT-PGA----PVKIIDFGFARLRPQSPGVPMQTPCFTLQYAAPELLA-----QQGYDESCDLWSLGVILYMMLSGQVPFQ-GASGQGGQSQAAEIMCKIREGRFSLDGEAWQGVS------------------------------EEAKELVRGLLTVDPAK--RLK-----LEGLRGSSW-L-----
---FELLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLKKA-SLKVRDRVRTKM----ERDILVEV-N----HPF--IVKLHYAFQT--EGKLYLILDFLRGG-DVFTRLS----KEVLFTEEDVKFYLAELALALDHLHQ-L-----GIVYRDLKPENILLD-EIGHI--------KLTDFGLSKES-VDQEKKAYSFCGTVEYMAPEVVN-----RRGHSQSADWWSYGVLMFEMLTGTLPFQ-GK-------DRNETMNMILKAKLGMPQFLS----------------------------------AEAQSLLRMLFKRNPAN--RLGS--EGVEEIKRHLFF------
---WEIIGELGDGAFGKVYKAQNKETSV---LAAAKVIDTK-SEEELEDYM--V----EIDILASC-D----HPN--IVKLLDAFYY--ENNLWILIEFCAGG-AVDAVML---ELERPLTESQIQVVCKQTLDALNYLHD-N-----KIIHRDLKAGNILFT-LDGDI--------KLADFGVSAKN-TRTIQRRDSFIGTPYWMAPEVVMCETSKDRPYDYKADVWSLGITLIEMAEIEPPHH-EL----NPMRVLLKIAKSEPPTLAQPSRWS----------------------------------SNFKDFLKKCLEKNVDA--RWT-----TSQLLQHPFV------
---WEIVGELGDGAFGKVYKAKNKETGA---LAAAKVIETK-SEEELEDYI--V----EIEILATC-D----HPY--IVKLLGAYYH--DGKLWIMIEFCPGG-AVDAIML---ELDRGLTEPQIQVVCRQMLEALNFLHS-K-----RIIHRDLKAGNVLMT-LEGDI--------RLADFGVSAKN-LKTLQKRDSFIGTPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHH-EL----NPMRVLLKIAKSDPPTLLTPSKWS----------------------------------VEFRDFLKIALDKNPET--RPS-----AAQLLEHPFV------
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