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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK LCK 203
TK ABL1 261
Other AURKA 133
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK5 4
TK CSF1R 582
TK EGFR 712
TK FGFR1 478
TK IGF1R 999
TK ITK 363
TK KIT 589
CMGC MAPK14 24
Other NEK2 8
CAMK NUAK1 55
TK PDGFRB 600
TK PTK6 191
TKL RAF1 349
TK ROS1 1945
TK SRC 270
TK SYK 371
TKL TGFBR1 205
TK YES1 277
---LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q-G-SM-SP---DAFL-A-EANLMKQL-QHQRLVRLYAVVT-------QEPIYIITEYMENGSLVDFLK-----TP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEER--------NYIHRDLRAANILVSD-----TLSCKIADFGLARLI-----E--DNEY-TAREGAKFPIKWTAPEAIN------YGT---FTIKSDVWSFGILLTEIVTHGR----------IPYPGM-----TNPEVIQNLERGYR----MVRPDNCP------------------------------EELYQLMRLCWKERPEDRPTFDYLRSV--L--
---ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-E-D-TM-EV---EEFL-K-EAAVMKEI-KHPNLVQLLGVCTR------EPPFYIITEFMTYGNLLDYLR-----EC-------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKK--------NFIHRDLAARNCLVGE-----NHLVKVADFGLSRLM-----T--GDTY-TAHAGAKFPIKWTAPESLA------YNK---FSIKSDVWAFGVLLWEIATYGM----------SPYPGI-----DLSQVYELLEKDYR----MERPEGCP------------------------------EKVYELMRACWQWNPSDRPSFAEIHQA--F--
---FEIGRPLGKGKFGNVYLAREK-------QSKFILALKVLFKA-QLEKAGV---EHQLRR-EVEIQSHL-RHPNILRLYGYFHD------ATRVYLILEYAPLGTVYRELQ----KLS----------------------------------------------------------------------------------------------------KFDEQRTATYITELANALSYCHSK--------RVIHRDIKPENLLLGS-----AGELKIADFGWSV-------HAPSSRR-TT---LCGTLDYLPPEMIE------GRM---HDEKVDLWSLGVLCYEFLV-GK----------PPFEAN--------TYQETYKRISR----VEFTFPDFVT----------------------------EGARDLISRLLKHNPSQRPMLREVLEHPWI--
---YTKIEKIGEGTYGVVYKGRHK-------TTGQVVAMKKIRLE-S-EEEGV---PSTAIR-EISLLKEL-RHPNIVSLQDVLMQ------DSRLYLIFEFLSM-DLKKYLDS-IPPGQ----------------------------------------------------------------------------------------------------YMDSSLVKSYLYQILQGIVFCHSR--------RVLHRDLKPQNLLIDD-----KGTIKLADFGLARAF-----GIPIRVY-TH---EVVTLWYRSPEVLL-----GSAR---YSTPVDIWSIGTIFAELAT-KK----------PLFHGD-----SEIDQLFRIFRALG----TPNNEVWPEVESLQDYKNTFPKWKP--GSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYF--
---FQKVEKIGEGTYGVVYKARNK-------LTGEVVALKKIRLD-T-ETEGV---PSTAIR-EISLLKEL-NHPNIVKLLDVIHT------ENKLYLVFEFLHQ-DLKKFMDA--SALT----------------------------------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHSH--------RVLHRDLKPQNLLINT-----EGAIKLADFGLARAF-----GVPVRTY-TH---EVVTLWYRAPEILL-----GCKY---YSTAVDIWSLGCIFAEMVT-RR----------ALFPGD-----SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR--QDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF--
---YEKLEKIGEGTYGTVFKAKNR-------ETHEIVALKRVRLD-D-DDEGV---PSSALR-EICLLKEL-KHKNIVRLHDVLHS------DKKLTLVFEFCDQ-DLKKYFD---SCNG----------------------------------------------------------------------------------------------------DLDPEIVKSFLFQLLKGLGFCHSR--------NVLHRDLKPQNLLINR-----NGELKLADFGLARAF-----GIPVRCY-SA---EVVTLWYRPPDVLF-----GAKL---YSTSIDMWSAGCIFAELANAGR----------PLFPGN-----DVDDQLKRIFRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT--TSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYF--
---LQFGKTLGAGAFGKVVEATAFG--LGKEDAVLKVAVKMLK-S-T-AH-ADE-KEALM-S-ELKIMSHLGQHENIVNLLGACTH------GGPVLVITEYCCYGDLLNFLR-----RKAEAMLGPSLSPGQDPEGGVDYKNIHLEKKYVRR------------------------------DSGFSSQGVDTYVEMRPVSTSSNDSFSEQDLDKEDGRPLELRDLLHFSSQVAQGMAFLASK--------NCIHRDVAARNVLLTN-----GHVAKIGDFGLARDI--M--N--DSNY-IVKGNARLPVKWMAPESIF------DCV---YTVQSDVWSYGILLWEIFSLGL----------NPYPGI-L---VNSKFYKLVKDGYQ----MAQPAFAP------------------------------KNIYSIMQACWALEPTHRPTFQQICSFL----
--F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E-A-TS-PKA-NKEIL-D-EAYVMASV-DNPHVCRLLGICL-------TSTVQLITQLMPFGCLLDYVR-----EH--------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDR--------RLVHRDLAARNVLVKT-----PQHVKITDFGLAKLL--G--A--EEKE-YHAEGGKVPIKWMALESIL------HRI---YTHQSDVWSYGVTVWELMTFGS----------KPYDGI-----PASEISSILEKGER----LPQPPICT------------------------------IDVYMIMVKCWMIDADSRPKFRELI----IEF
--L-VLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLK-S-D-AT-EKD-LSDLI-S-EMEMMKMIGKHKNIINLLGACTQ------DGPLYVIVEYASKGNLREYLQ-----ARRPPGLEY-----------------------------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASK--------KCIHRDLAARNVLVTE-----DNVMKIADFGLARDI--H--H--IDYY-KKTTNGRLPVKWMAPEALF------DRI---YTHQSDVWSFGVLLWEIFTLGG----------SPYPGV-----PVEELFKLLKEGHR----MDKPSNCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----
---ITMSRELGQGSFGMVYEGVAKG--VVKDEPETRVAIKTVN-E-A-AS-MRE-RIEFL-N-EASVMKEF-NCHHVVRLLGVVSQ------GQPTLVIMELMTRGDLKSYLR-----SLRPEM-----------------------------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNAN--------KFVHRDLAARNCMVAE-----DFTVKIGDFGMTRDI--Y--E--TDYY-RKGGKGLLPVRWMSPESLK------DGV---FTTYSDVWSFGVVLWEIATLAE----------QPYQGL-----SNEQVLRFVMEGGL----LDKPDNCP------------------------------DMLFELMRMCWQYNPKMRPSFLEIISS--I--
---LTFVQEIGSGQFGLVHLGYWL--------NKDKVAIKTIR-E-G-AM-SE---EDFI-E-EAEVMMKL-SHPKLVQLYGVCLE------QAPICLVFEFMEHGCLSDYLR-----TQ--------------------------------------------------------------------------------------------------RGLFAAETLLGMCLDVCEGMAYLEEA--------CVIHRDLAARNCLVGE-----NQVIKVSDFGMTRFV-----L--DDQY-TSSTGTKFPVKWASPEVFS------FSR---YSSKSDVWSFGVLMWEVFSEGK----------IPYENR-----SNSEVVEDISTGFR----LYKPRLAS------------------------------THVYQIMNHCWKERPEDRPAFSRLLRQL----
---LSFGKTLGAGAFGKVVEATAYG--LIKSDAAMTVAVKMLK-P-S-AH-LTE-REALM-S-ELKVLSYLGNHMNIVNLLGACTI------GGPTLVITEYCCYGDLLNFLR-----RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM-----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASK--------NCIHRDLAARNILLTH-----GRITKICDFGLARDI--K--N--DSNY-VVKGNARLPVKWMAPESIF------NCV---YTFESDVWSYGIFLWELFSLGS----------SPYPGM-P---VDSKFYKMIKEGFR----MLSPEHAP------------------------------AEMYDIMKTCWDADPLKRPTFKQIVQL--I--
---YQNLSPVGSGAYGSVCAAFDT-------KTGLRVAVKKLSRP-F-QSIIH---AKRTYR-ELRLLKHM-KHENVIGLLDVFTPARSLEEFNDVYLVTHLMGA-DLNNIVK-----CQ----------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSA--------DIIHRDLKPSNLAVNE-----DCELKILDFGLAR--------HTDDEM-TG---YVATRWYRAPEIML-----NWMH---YNQTVDIWSVGCIMAELLT-GR----------TLFPGT-----DHIDQLKLILRLVG----TPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYF--
---YEVLYTIGTGSYGRCQKIRRK-------SDGKILVWKELDYG-S-MTEAE---KQMLVS-EVNLLREL-KHPNIVRYYDRIIDR----TNTTLYIVMEYCEGGDLASVITKGTKERQ----------------------------------------------------------------------------------------------------YLDEEFVLRVMTQLTLALKECHRRSD---GGHTVLHRDLKPANVFLDG-----KQNVKLGDFGLARIL-----NHDTSFA-KT---FVGTPYYMSPEQMN------RMS---YNEKSDIWSLGCLLYELCA-LM----------PPFTAF--------SQKELAGKIRE----GKFRRIPYRY---S------------------------DELNEIITRMLNLKDYHRPSVEEILENPL-I-
---YELQETLGKGTYGKVKRATER-------FSGRVVAIKSIRKD-K-IKDEQ---DMVHIRREIEIMSSL-NHPHIISIYEVFEN------KDKIVIIMEYASKGELYDYIS----ERR----------------------------------------------------------------------------------------------------RLSERETRHFFRQIVSAVHYCHKN--------GVVHRDLKLENILLDD-----NCNIKIADFGLSNL------YQKDKFL-QT---FCGSPLYASPEIVN-----GRPY---RGPEVDSWALGVLLYTLVY-GT----------MPFDGF--------DHKNLIRQISS----GEYREPTQP-----------------------------SDARGLIRWMLMVNPDRRATIEDIANHWW-V-
--L-VLGRTLGSGAFGQVVEATAHG--LSHSQATMKVAVKMLK-S-T-AR-SSE-KQALM-S-ELKIMSHLGPHLNVVNLLGACTK------GGPIYIITEYCRYGDLVDYLH-----RNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASK--------NCVHRDLAARNVLICE-----GKLVKICDFGLARDI--M--R--DSNY-ISKGSTFLPLKWMAPESIF------NSL---YTTLSDVWSFGILLWEIFTLGG----------TPYPEL-P---MNEQFYNAIKRGYR----MAQPAHAS------------------------------DEIYEIMQKCWEEKFEIRPPFSQLVL---LL-
--F-TLCRKLGSGYFGEVFEGLWK--------DRVQVAIKVIS-R-D-NL-LH--QQMLQ-S-EIQAMKKL-RHKHILALYAVVSV------GDPVYIITELMAKGSLLELLR-----DS-------------------------------------------------------------------------------------------------DEKVLPVSELLDIAWQVAEGMCYLESQ--------NYIHRDLAARNILVGE-----NTLCKVGDFGLARLI--------KEDV-YLSHDHNIPYKWTAPEALS------RGH---YSTKSDVWSFGILLHEMFSRGQ----------VPYPGM-----SNHEAFLRVDAGYR----MPCPLECP------------------------------PSVHKLMLTCWCRDPEQRPCFKALRERL----
-VM--LSTRIGSGSFGTVYKGKWH----------GDVAVKILK-VVD-PT-PEQ-FQAFR-N-EVAVLRKT-RHVNILLFMGYMT-------KDNLAIVTQWCEGSSLYKHLH-----VQ--------------------------------------------------------------------------------------------------ETKFQMFQLIDIARQTAQGMDYLHAK--------NIIHRDMKSNNIFLHE-----GLTVKIGDFGLATVK--S--R--WSGS-QQVEQPTGSVLWMAPEVIR------MQDNNPFSFQSDVYSYGIVLYELMT-GE----------LPYSHI-N---NRDQIIFMVGRGYASPDLSKLYKNCP------------------------------KAMKRLVADCVKKVKEERPLFPQILSS--I--
-LT--LRLLLGSGAFGEVYEGTAVDI-LGVGSGEIKVAVKTLK-K-G-ST-DQE-KIEFL-K-EAHLMSKF-NHPNILKQLGVCLL------NEPQYIILELMEGGDLLTYLR-----KARM--------------------------------------------------------------------------------------------ATFYGPLLTLVDLVDLCVDISKGCVYLERM--------HFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDI--Y--K--NDYY-RKRGEGLLPVRWMAPESLM------DGI---FTTQSDVWSFGILIWEILTLGH----------QPYPAH-----SNLDVLNYVQTGGR----LEPPRNCP------------------------------DDLWNLMTQCWAQEPDQRPTFHRIQDQL----
--L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P-G-TM-SP---EAFL-Q-EAQVMKKL-RHEKLVQLYAVVS-------EEPIYIVTEYMSKGSLLDFLK-----GE-------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERM--------NYVHRDLRAANILVGE-----NLVCKVADFGLARLI-----E--DNEY-TARQGAKFPIKWTAPEAAL------YGR---FTIKSDVWSFGILLTELTTKGR----------VPYPGM-----VNREVLDQVERGYR----MPCPPECP------------------------------ESLHDLMCQCWRKEPEERPTFEYLQAF--L--
TLE---DKELGSGNFGTVKKGYYQ-----MKKVVKTVAVKILK-N-E-AN-DPALKDELL-A-EANVMQQL-DNPYIVRMIGICE-------AESWMLVMEMAELGPLNKYLQ-----QN---------------------------------------------------------------------------------------------------RHVKDKNIIELVHQVSMGMKYLEES--------NFVHRDLAARNVLLVT-----QHYAKISDFGLSKAL--R--A--DENYYKAQTHGKWPVKWYAPECIN------YYK---FSSKSDVWSFGVLMWEAFSYGQ----------KPYRGM-----KGSEVTAMLEKGER----MGCPAGCP------------------------------REMYDLMNLCWTYDVENRPGFAAVE----LRL
-IV--LQESIGKGRFGEVWRGKWR-------GEE--VAVKIFSSR-E-ERSWF---REA----EIYQTVML-RHENILGFIAADNKDN--GTWTQLWLVSDYHEHGSLFDYLN-----RY----------------------------------------------------------------------------------------------------TVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKK-----NGTCCIADLGLAVRHDS-ATDTIDIAP-NH---RVGTKRYMAPEVLDDSINMKHFE---SFKRADIYAMGLVFWEIAR-RCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKL----RPNIPNRWQSCEAL------------------------RVMAKIMRECWYANGAARLTALRIKK----TL
--L-RLEVKLGQGCFGEVWMGTWN--------GTTKVAIKTLK-P-G-TM-MP---EAFL-Q-EAQIMKKL-RHDKLVPLYAVVS-------EEPIYIVTEFMSKGSLLDFLK-----EG-------------------------------------------------------------------------------------------------DGKYLKLPQLVDMAAQIADGMAYIERM--------NYIHRDLRAANILVGE-----NLVCKIADFGLARLI-----E--DNEY-TARQGAKFPIKWTAPEAAL------YGR---FTIKSDVWSFGILQTELVTKGR----------VPYPGM-----VNREVLEQVERGYR----MPCPQGCP------------------------------ESLHELMNLCWKKDPDERPTFEYIQSFL----
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