|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK ROS1 203
TK ALK 1116
TK CSF1R 582
TK DDR1 572
TK EGFR 712
TK FER 563
TK FES 561
TK INSR 1023
TK JAK1 875
TK JAK2 849
TK JAK3 822
TK LTK 510
TKL MAP3K7 36
TK MET 1078
TK MUSK 575
TK NTRK2 538
TK NTRK3 538
TK RET 724
TKL RIPK2 18
TKL RIPK4 22
TKL TNNI3K 463
--LT--LRLLLGSGAFGEVYEGTAVDI----------LGVGSGEIKVAVKTLK-K--GST-DQEKIEFL-K-EAHLMSKF-NHPNILKQLGVCL--LNEP--QYIILELMEGGDLLTYLR-KARM--------------------------------------------------------------ATFYG-PLL---TLVDLVDLCVDISKGCVYLER--M--H---FIHRDLAARNCLVSVKDYTSPR-IVKIGDFGLARDI---Y---K--NDYY--------------RKRGEGLLPVRWMAPESLM---DGI-FTTQSDVWSFGILIWEILTLGH-QP---------------YPAH---SNLDVLNYV----QTGGR---LEPPRNCP------DDLWNLMTQCWAQEPDQRPTFHRIQDQL------
---I-TLIRGLGHGAFGEVYEGQVSG-----------MPNDPSPLQVAVKTLP-E--VCS-EQDELDFL-M-EALIISKF-NHQNIVRCIGVSL--QSLP--RFILLELMAGGDLKSFLR-ETRP--------------------------------------------------------------RPSQP-SSL---AMLDLLHVARDIACGCQYLEE--N--H---FIHRDIAARNCLLTCP--GPGR-VAKIGDFGMARDI---Y---R--ASYY--------------RKGGCAMLPVKWMPPEAFM---EGI-FTSKTDTWSFGVLLWEIFSLGY-MP---------------YPSK---SNQEVLEFV----TSGGR---MDPPKNCP------GPVYRIMTQCWQHQPEDRPNFAIILER-I-----
----LQFGKTLGAGAFGKVVEATAFG-----------LGKEDAVLKVAVKMLK-S--TAH-ADEKEALM-S-ELKIMSHLGQHENIVNLLGACT--HGGP--VLVITEYCCYGDLLNFLR-RKAEAMLGPSLSPGQDPEGGVDYKNIHLEKKYVRRDSGFSSQGVDTYVEMRPVSTSSNDSFSEQDLDKEDG-RPL---ELRDLLHFSSQVAQGMAFLAS--K--N---CIHRDVAARNVLLTN-----GH-VAKIGDFGLARDI---M---N--DSNY--------------IVKGNARLPVKWMAPESIF---DCV-YTVQSDVWSYGILLWEIFSLGL-NP---------------YPGI-L-VNSKFYKLV----KDGYQ---MAQPAFAP------KNIYSIMQACWALEPTHRPTFQQICSFL------
----LRFKEKLGEGQFGEVHLCEVDSPQDLVSLDFPLNVRKGHPLLVAVKILR-P--DAT-KNARNDFL-K-EVKIMSRL-KDPNIIRLLGVCV--QDDP--LCMITDYMENGDLNQFLS-AHQLEDKAAE--------------------------------------------------GAPGDGQAAQG-PTI---SYPMLLHVAAQIASGMRYLAT--L--N---FVHRDLATRNCLVGE-----NF-TIKIADFGMSRNL---Y---A--GDYY--------------RVQGRAVLPIRWMAWECIL---MGK-FTTASDVWAFGVTLWEVLMLCRAQP---------------FGQL---TDEQVIENAGEFFRDQGRQVYLSRPPACP------QGLYELMLRCWSRESEQRPPFSQLHRFL------
---F-KKIKVLGSGAFGTVYKGLWIP------------EGEKVKIPVAIKELR-E--ATS-PKANKEIL-D-EAYVMASV-DNPHVCRLLGICL---TST--VQLITQLMPFGCLLDYVR-EH--------------------------------------------------------------------K-DNI---GSQYLLNWCVQIAKGMNYLED--R--R---LVHRDLAARNVLVKT-----PQ-HVKITDFGLAKLL---G---A--EEKE--------------YHAEGGKVPIKWMALESIL---HRI-YTHQSDVWSYGVTVWELMTFGS-KP---------------YDGI---PASEISSIL----EKGER---LPQPPICT------IDVYMIMVKCWMIDADSRPKFRELI---IEF---
--VI--LGELLGKGNFGEVYKGTLK-----------------DKTSVAVKTCK-E--DLP-QELKIKFL-Q-EAKILKQY-DHPNIVKLIGVCT--QRQP--VYIIMELVSGGDFLTFLR-RK--------------------------------------------------------------------K-DEL---KLKQLVKFSLDAAAGMLYLES--K--N---CIHRDLAARNCLVGE-----NN-VLKISDFGMSRQE-------D--GGVY--------------SSSGLKQIPIKWTAPEALN---YGR-YSSESDVWSFGILLWETFSLGV-CP---------------YPGM---TNQQAREQV----ERGYR---MSAPQHCP------EDISKIMMKCWDYKPENRPKFSELQKEL------
---L-VLGEQIGRGNFGEVFSGRLR----------------ADNTLVAVKSCR-E--TLP-PDLKAKFL-Q-EARILKQY-SHPNIVRLIGVCT--QKQP--IYIVMELVQGGDFLTFLR-TE--------------------------------------------------------------------G-ARL---RVKTLLQMVGDAAAGMEYLES--K--C---CIHRDLAARNCLVTE-----KN-VLKISDFGMSREE---A---D--GVYA--------------ASGGLRQVPVKWTAPEALN---YGR-YSSESDVWSFGILLWETFSLGA-SP---------------YPNL---SNQQTREFV----EKGGR---LPCPELCP------DAVFRLMEQCWAYEPGQRPSFSTIYQEL------
----ITLLRELGQGSFGMVYEGNARD-----------IIKGEAETRVAVKTVN-E--SAS-LRERIEFL-N-EASVMKGF-TCHHVVRLLGVVS--KGQP--TLVVMELMAHGDLKSYLR-SLRPEA-----------------------------------------------------------ENNPGR-PPP---TLQEMIQMAAEIADGMAYLNA--K--K---FVHRDLAARNCMVAH-----DF-TVKIGDFGMTRDI---Y---E--TDYY--------------RKGGKGLLPVRWMAPESLK---DGV-FTTSSDMWSFGVVLWEITSLAE-QP---------------YQGL---SNEQVLKFV----MDGGY---LDQPDNCP------ERVTDLMRMCWQFNPKMRPTFLEIVNLL------
---L-KRIRDLGEGHFGKVELCRYDP------------EGDNTGEQVAVKSLK-P--ESG-GNHIADLK-K-EIEILRNL-YHENIVKYKGICT-ED-GGNGIKLIMEFLPSGSLKEYLP-KN--------------------------------------------------------------------K-NKI---NLKQQLKYAVQICKGMDYLGS--R--Q---YVHRDLAARNVLVES-----EH-QVKIGDFGLTKAI--ET---D--KEYY--------------TVKDDRDSPVFWYAPECLM---QSK-FYIASDVWSFGVTLHELLTYCD-SDSSPMALF-LKMIGPTHGQM---TVTRLVNTL----KEGKR---LPCPPNCP------DEVYQLMRKCWEFQPSNRTSFQNLIE--GF----
----LKFLQQLGKGNFGSVEMCRYDP------------LQDNTGEVVAVKKLQ-H--STE--EHLRDFE-R-EIEILKSL-QHDNIVKYKGVCYSAGRRN--LKLIMEYLPYGSLRDYLQ-KH--------------------------------------------------------------------K-ERI---DHIKLLQYTSQICKGMEYLGT--K--R---YIHRDLATRNILVEN-----EN-RVKIGDFGLTKVL--PQ---D--KEYY--------------KVKEPGESPIFWYAPESLT---ESK-FSVASDVWSFGVVLYELFTYIE-KSKSPPAEFMRMIGNDKQGQM---IVFHLIELL----KNNGR---LPRPDGCP------DEIYMIMTECWNNNVNQRPSFRDLA---LRV---
---L-KYISQLGKGNFGSVELCRYDP------------LGDNTGALVAVKQLQ-H--SGP-DQ-QRDFQ-R-EIQILKAL-HSDFIVKYRGVSYGPGRQS--LRLVMEYLPSGCLRDFLQ-RH--------------------------------------------------------------------R-ARL---DASRLLLYSSQICKGMEYLGS--R--R---CVHRDLAARNILVES-----EA-HVKIADFGLAKLL--PL---D--KDYY--------------VVREPGQSPIFWYAPESLS---DNI-FSRQSDVWSFGVVLYELFTYCD-KSCSPSAE--FLRMMGCERDV-P-ALCRLLELL----EEGQR---LPAPPACP------AEVHELMKLCWAPSPQDRPSFSALGP--QL----
---V-TLLRALGHGAFGEVYEGLVIG-----------LPGDSSPLQVAIKTLP-E--LCS-PQDELDFL-M-EALIISKF-RHQNIVRCVGLSL--RATP--RLILLELMSGGDMKSFLR-HSRP--------------------------------------------------------------HLGQP-SPL---VMRDLLQLAQDIAQGCHYLEE--N--H---FIHRDIAARNCLLSCA--GPSR-VAKIGDFGMARDI---Y---R--ASYY--------------RRGDRALLPVKWMPPEAFL---EGI-FTSKTDSWSFGVLLWEIFSLGY-MP---------------YPGR---TNQEVLDFV----VGGGR---MDPPRGCP------GPVYRIMTQCWQHEPELRPSFASILERL------
----IEVEEVVGRGAFGVVCKAKWR------------------AKDVAIKQIE------S-ESERKAFI-V-ELRQLSRV-NHPNIVKLYGACL----NP--VCLVMEYAEGGSLYNVLH-GA-------------------------------------------------------------------EPLPYY---TAAHAMSWCLQCSQGVAYLHS--M--QPKALIHRDLKPPNLLLVA-----GGTVLKICDFGTACDI-------------Q--------------THMTNNKGSAAWMAPEVFE---GSN-YSEKCDVFSWGIILWEVITR-R-KP---------------FDEIGG-PAFRIMWAV----HNGTR---PPLIKNLP------KPIESLMTRCWSKDPSQRPSMEEIVKI-M-----
----VHFNEVIGRGHFGCVYHGTLL-------------DNDGKKIHCAVKSLN-R--ITD-IGEVSQFL-T-EGIIMKDF-SHPNVLSLLGICL-RSEGS--PLVVLPYMKHGDLRNFIR-NE--------------------------------------------------------------------T-HNP---TVKDLIGFGLQVAKGMKYLAS--K--K---FVHRDLAARNCMLDE-----KF-TVKVADFGLARDM---Y---D--KEYYS------------VHNKTGAKLPVKWMALESLQ---TQK-FTTKSDVWSFGVLLWELMTRGA-PP---------------YPDV---NTFDITVYL----LQGRR---LLQPEYCP------DPLYEVMLKCWHPKAEMRPSFSELVSR-I-----
---I-EYVRDIGEGAFGRVFQARAPG-----------LLPYEPFTMVAVKMLK-E--EAS-ADMQADFQ-R-EAALMAEF-DNPNIVKLLGVCA--VGKP--MCLLFEYMAYGDLNEFLR-SMSPHTVCSLSHS---------------------------------------------DLSMRAQVSSPGP-PPL---SCAEQLCIARQVAAGMAYLSE--R--K---FVHRDLATRNCLVGE-----NM-VVKIADFGLSRNI---Y---S--ADYY--------------KANENDAIPIRWMPPESIF---YNR-YTTESDVWAYGVVLWEIFSYGL-QP---------------YYGM---AHEEVIYYV----RDGNI---LSCPENCP------VELYNLMRLCWSKLPADRPSFTSIHRIL------
---I-VLKRELGEGAFGKVFLAECYN-----------LCPEQDKILVAVKTLK-D--ASD-NA-RKDFH-R-EAELLTNL-QHEHIVKFYGVCV--EGDP--LIMVFEYMKHGDLNKFLR-AHGPDAV--------------------------------------------------------LMAEGNPP-TEL---TQSQMLHIAQQIAAGMVYLAS--Q--H---FVHRDLATRNCLVGE-----NL-LVKIGDFGMSRDV---Y---S--TDYY--------------RVGGHTMLPIRWMPPESIM---YRK-FTTESDVWSLGVVLWEIFTYGK-QP---------------WYQL---SNNEVIECI----TQGRV---LQRPRTCP------QEVYELMLGCWQREPHMRKNIKGIHTL-L-----
---I-VLKRELGEGAFGKVFLAECYN-----------LSPTKDKMLVAVKALK-D--PTL-AA-RKDFQ-R-EAELLTNL-QHEHIVKFYGVCG--DGDP--LIMVFEYMKHGDLNKFLR-AHGPDAMIL-----------------------------------------------------VDGQPRQAK-GEL---GLSQMLHIASQIASGMVYLAS--Q--H---FVHRDLATRNCLVGA-----NL-LVKIGDFGMSRDV---Y---S--TDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIM---YRK-FTTESDVWSFGVILWEIFTYGK-QP---------------WFQL---SNTEVIECI----TQGRV---LERPRVCP------KEVYDVMLGCWQREPQQRLNIKEIYKIL------
---L-VLGKTLGEGEFGKVVKATAFH-----------LKGRAGYTTVAVKMLK-E--NAS-PSELRDLL-S-EFNVLKQV-NHPHVIKLYGACS--QDGP--LLLIVEYAKYGSLRGFLR-ESRKVGPGYLGSG---------------------------------------------GSRNSSSLDHPDE-RAL---TMGDLISFAWQISQGMQYLAE--M--K---LVHRDLAARNILVAE-----GR-KMKISDFGLSRDV---Y---E--EDSY--------------VKRSQGRIPVKWMAIESLF---DHI-YTTQSDVWSFGVLLWEIVTLGG-NP---------------YPGI---PPERLFNLL----KTGHR---MERPDNCS------EEMYRLMLQCWKQEPDKRPVFADISKDL------
-LAD---LRYLSRGASGTVSSARHA----------------DWRVQVAVKHLHIH--TPLLDS--ERKDVLREAEILHKA-RFSYILPILGICN--EPEF--LGIVTEYMPNGSLNELLHRKT-----------------------------------------------------------------------EYPDVAWPLRFRILHEIALGVNYLHN--MTPP---LLHHDLKTQNILLDN-----EF-HVKIADFGLSKWRMM--SLSQSRSSKS--------------AP---EGGTIIYMPPENYEPGQKSR-ASIKHDIYSYAVITWEVLS-RK-QP---------------FEDVT-NPLQIMYSVS----QGHRP---VINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCL----IEL--
FTGW----EKVGSGGFGQVYKVRHV----------------HWKTWLAIKCSPSL--HVDDRE--RMELLE-EAKKMEMA-KFRYILPVYGIC----REP--VGLVMEYMETGSLEKLLA-SE-----------------------------------------------------------------------PL---PWDLRFRIIHETAVGMNFLHC--MAPP---LLHLDLKPANILLDA-----HY-HVKISDFGLAKCNG----LSHSHDLSM--------------DG---LFGTIAYLPPERIR-EKSRL-FDTKHDVYSFAIVIWGVLT-QK-KP---------------FADE--KNILHIMVKV----VKGHR---PELPPVCRARPRACSHLIRLMQRCWQGDPRVRPTFQ-------GNGLN
----IEFHEIIGSGSFGKVYKGRCR------------------NKIVAIKRYR-ANTYCS-KSDVDMFC-R-EVSILCQL-NHPCVIQFVGACL-NDPSQ--FAIVTQYISGGSLFSLLH-EQ--------------------------------------------------------------------K-RIL---DLQSKLIIAVDVAKGMEYLHNLTQ--P---IIHRDLNSHNILLYE-----DG-HAVVADFGESRFL---Q---S--LD-E--------------DNMTKQPGNLRWMAPEVFT---QCTRYTIKADVFSYALCLWEILT-GE-IP---------------FAHL---KPAAAAADM---AYHHIR---PPIGYSIP------KPISSLLIRGWNACPEGRPEFSEVVMKL------
|