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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK ROS1 203
TK ABL1 261
TK ALK 1116
TK BMX 422
TK BTK 402
CMGC CDK2 4
TK EGFR 712
TK EPHB1 619
TK EPHB4 615
TK FGFR1 478
TK FGFR2 481
TK FGFR3 472
TK FGFR4 467
TK FYN 271
TK HCK 241
TK IGF1R 999
TK INSR 1023
TK JAK2 849
TK KIT 589
TK LCK 245
TK LYN 247
CMGC MAPK14 24
TK NTRK2 538
TK PDGFRA 593
TK PDGFRB 600
TK RET 724
TK SRC 270
TK SYK 371
TK YES1 277
-LT--LRLLLGSGAFGEVYEGTAVDI-LGVGSGEIKVAVKTLK-KGST-DQE-KIEFL-KEAHLMSKF-NHPNILKQLGVCL--L------NEPQYIILELMEGGDLLTYLR---KARM--------------------------------------------------------------------------------------------ATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDI---YK--NDYY-RKRGEGLLPVRWMAPESLM-DGIFTTQSDVWSFGILIWEILTLGHQP---------------YPAH-SNLDVLNYVQTGGRLEPPRNCP------------------------------DDLWNLMTQCWAQEPDQRPTFHRIQDQL----
---ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-EDTM-EV---EEFL-KEAAVMKEI-KHPNLVQLLGVCT--R------EPPFYIITEFMTYGNLLDYLR---EC-------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGE-----NHLVKVADFGLSRLM----T--GDTY-TAHAGAKFPIKWTAPESLA-YNKFSIKSDVWAFGVLLWEIATYGMSP---------------YPGI-DLSQVYELLEKDYRMERPEGCP------------------------------EKVYELMRACWQWNPSDRPSFAEIHQA--F--
--I-TLIRGLGHGAFGEVYEGQVSG--MPNDPSPLQVAVKTLP-EVCS-EQD-ELDFL-MEALIISKF-NHQNIVRCIGVSL--Q------SLPRFILLELMAGGDLKSFLR---ETRP--------------------------------------------------------------------------------------------RPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCP--GPGRVAKIGDFGMARDI---YR--ASYY-RKGGCAMLPVKWMPPEAFM-EGIFTSKTDTWSFGVLLWEIFSLGYMP---------------YPSK-SNQEVLEFVTSGGRMDPPKNCP------------------------------GPVYRIMTQCWQHQPEDRPNFAIILER--I--
---ITLLKELGSGQFGVVQLGKWK--------GQYDVAVKMIK-EGSM-SE---DEFF-QEAQTMMKL-SHPKLVKFYGVCS--K------EYPIYIVTEYISNGCLLNYLR---SH--------------------------------------------------------------------------------------------------GKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDR-----DLCVKVSDFGMTRYV----L--DDQY-VSSVGTKFPVKWSAPEVFH-YFKYSSKSDVWAFGILMWEVFSLGKQP---------------YDLY-DNSQVVLKVSQGHRLYRPHLAS------------------------------DTIYQIMYSCWHELPEKRPTFQQLLSS--I--
---LTFLKELGTGQFGVVKYGKWR--------GQYDVAIKMIK-EGSM-SE---DEFI-EEAKVMMNL-SHEKLVQLYGVCT--K------QRPIFIITEYMANGCLLNYLR---EM--------------------------------------------------------------------------------------------------RHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVND-----QGVVKVSDFGLSRYV----L--DDEY-TSSVGSKFPVRWSPPEVLM-YSKFSSKSDIWAFGVLMWEIYSLGKMP---------------YERF-TNSETAEHIAQGLRLYRPHLAS------------------------------EKVYTIMYSCWHEKADERPTFKILLSN--I--
---FQKVEKIGEGTYGVVYKARNK-------LTGEVVALKKIRLDTETEGV---PSTAIREISLLKEL-NHPNIVKLLDVIH--T------ENKLYLVFEFLHQ-DLKKFMDASALT----------------------------------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINT-----EGAIKLADFGLARAF----GVPVRTY-TH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-RRAL---------------FPGD-SEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWAR--QDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF--
--F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-EATS-PKA-NKEIL-DEAYVMASV-DNPHVCRLLGICL---------TSTVQLITQLMPFGCLLDYVR---EH--------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKT-----PQHVKITDFGLAKLL---GA--EEKE-YHAEGGKVPIKWMALESIL-HRIYTHQSDVWSYGVTVWELMTFGSKP---------------YDGI-PASEISSILEKGERLPQPPICT------------------------------IDVYMIMVKCWMIDADSRPKFRELI----IEF
--V-KIEEVIGAGEFGEVYKGRLK----LPGKREIYVAIKTLK-AGYS-EKQ-RRDFL-SEASIMGQF-DHPNIIRLEGVVT--K------SRPVMIITEFMENGALDSFLR---QN--------------------------------------------------------------------------------------------------DGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNS-----NLVCKVSDFGLSRYLQDDTS--DPTY-TSSLGGKIPVRWTAPEAIA-YRKFTSASDVWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAIEQDYRLPPPMDCP------------------------------AALHQLMLDCWQKDRNSRPRFAEIVNTL----
--V-KIEEVIGAGEFGEVCRGRLK----APGKKESCVAIKTLK-GGYT-ERQ-RREFL-SEASIMGQF-EHPNIIRLEGVVT--N------SMPVMILTEFMENGALDSFLR---LN--------------------------------------------------------------------------------------------------DGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNS-----NLVCKVSDFGLSRFLEENSS--DPTY-TSSLGGKIPIRWTAPEAIA-FRKFTSASDAWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAIEQDYRLPPPPDCP------------------------------TSLHQLMLDCWQKDRNARPRFPQVVSAL----
--L-VLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLK-SDAT-EKD-LSDLI-SEMEMMKMIGKHKNIINLLGACT--Q------DGPLYVIVEYASKGNLREYLQ---ARRPPGLEY-----------------------------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTE-----DNVMKIADFGLARDI---HH--IDYY-KKTTNGRLPVKWMAPEALF-DRIYTHQSDVWSFGVLLWEIFTLGGSP---------------YPGV-PVEELFKLLKEGHRMDKPSNCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----
---LTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLK-DDAT-EKD-LSDLV-SEMEMMKMIGKHKNIINLLGACT--Q------DGPLYVIVEYASKGNLREYLR---ARRPPGMEY-----------------------------------------------------------------------------------SYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTE-----NNVMKIADFGLARDI---NN--IDYY-KKTTNGRLPVKWMAPEALF-DRVYTHQSDVWSFGVLMWEIFTLGGSP---------------YPGI-PVEELFKLLKEGHRMDKPANCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----
---LTLGKPLGEGCFGQVVMAEAIGIDKDRAAKPVTVAVKMLK-DDAT-DKD-LSDLV-SEMEMMKMIGKHKNIINLLGACT--Q------GGPLYVLVEYAAKGNLREFLR---ARRPPGLDY-----------------------------------------------------------------------------------SFDTCKPPEEQLTFKDLVSCAYQVARGMEYLASQKCIHRDLAARNVLVTE-----DNVMKIADFGLARDV---HN--LDYY-KKTTNGRLPVKWMAPEALF-DRVYTHQSDVWSFGVLLWEIFTLGGSP---------------YPGI-PVEELFKLLKEGHRMDKPANCT------------------------------HDLYMIMRECWHAAPSQRPTFKQLVEDL----
--L-VLGKPLGEGCFGQVVRAEAFGMDPARPDQASTVAVKMLK-DNAS-DKD-LADLV-SEMEVMKLIGRHKNIINLLGVCT--Q------EGPLYVIVECAAKGNLREFLR---ARRPPGPDL-----------------------------------------------------------------------------------SPDGPRSSEGPLSFPVLVSCAYQVARGMQYLESRKCIHRDLAARNVLVTE-----DNVMKIADFGLARGV---HH--IDYY-KKTSNGRLPVKWMAPEALF-DRVYTHQSDVWSFGILLWEIFTLGGSP---------------YPGI-PVEELFSLLREGHRMDRPPHCP------------------------------PELYGLMRECWHAAPSQRPTFKQLVEAL----
---LQLIKRLGNGQFGEVWMGTWN--------GNTKVAIKTLK-PGTM-SP---ESFL-EEAQIMKKL-KHDKLVQLYAVVS---------EEPIYIVTEYMNKGSLLDFLK---DG-------------------------------------------------------------------------------------------------EGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGN-----GLICKIADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILLTELVTKGRVP---------------YPGM-NNREVLEQVERGYRMPCPQDCP------------------------------ISLHELMIHCWKKDPEERPTFEYLQSFL----
---LKLEKKLGAGQFGEVWMATYN--------KHTKVAVKTMK-PGSM-SV---EAFL-AEANVMKTL-QHDKLVKLHAVVT---------KEPIYIITEFMAKGSLLDFLK---SD-------------------------------------------------------------------------------------------------EGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSA-----SLVCKIADFGLARVI----E--DNEY-TAREGAKFPIKWTAPEAIN-FGSFTIKSDVWSFGILLMEIVTYGRIP---------------YPGM-SNPEVIRALERGYRMPRPENCP------------------------------EELYNIMMRCWKNRPEERPTFEYIQSV--L--
---ITMSRELGQGSFGMVYEGVAKG--VVKDEPETRVAIKTVN-EAAS-MRE-RIEFL-NEASVMKEF-NCHHVVRLLGVVS--Q------GQPTLVIMELMTRGDLKSYLR---SLRPEM-----------------------------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAE-----DFTVKIGDFGMTRDI---YE--TDYY-RKGGKGLLPVRWMSPESLK-DGVFTTYSDVWSFGVVLWEIATLAEQP---------------YQGL-SNEQVLRFVMEGGLLDKPDNCP------------------------------DMLFELMRMCWQYNPKMRPSFLEIISS--I--
---ITLLRELGQGSFGMVYEGNARD--IIKGEAETRVAVKTVN-ESAS-LRE-RIEFL-NEASVMKGF-TCHHVVRLLGVVS--K------GQPTLVVMELMAHGDLKSYLR---SLRPEA-----------------------------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAH-----DFTVKIGDFGMTRDI---YE--TDYY-RKGGKGLLPVRWMAPESLK-DGVFTTSSDMWSFGVVLWEITSLAEQP---------------YQGL-SNEQVLKFVMDGGYLDQPDNCP------------------------------ERVTDLMRMCWQFNPKMRPTFLEIVNLL----
---LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-HSTE--EH-LRDFE-REIEILKSL-QHDNIVKYKGVCYSAG------RRNLKLIMEYLPYGSLRDYLQ---KH--------------------------------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVEN-----ENRVKIGDFGLTKVL--PQD--KEYY-KVKEPGESPIFWYAPESLT-ESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM-IVFHLIELLKNNGRLPRPDGCP------------------------------DEIYMIMTECWNNNVNQRPSFRDLA----LRV
---LSFGKTLGAGAFGKVVEATAYG--LIKSDAAMTVAVKMLK-PSAH-LTE-REALM-SELKVLSYLGNHMNIVNLLGACT--I------GGPTLVITEYCCYGDLLNFLR---RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM-----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTH-----GRITKICDFGLARDI---KN--DSNY-VVKGNARLPVKWMAPESIF-NCVYTFESDVWSYGIFLWELFSLGSSP---------------YPGMPVDSKFYKMIKEGFRMLSPEHAP------------------------------AEMYDIMKTCWDADPLKRPTFKQIVQL--I--
---LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-QGSM-SP---DAFL-AEANLMKQL-QHQRLVRLYAVVT---------QEPIYIITEYMENGSLVDFLK---TP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSD-----TLSCKIADFGLARLI----E--DNEY-TAREGAKFPIKWTAPEAIN-YGTFTIKSDVWSFGILLTEIVTHGRIP---------------YPGM-TNPEVIQNLERGYRMVRPDNCP------------------------------EELYQLMRLCWKERPEDRPTFDYLRSV--L--
---IKLVKRLGAGQFGEVWMGYYN--------NSTKVAVKTLK-PGTM-SV---QAFL-EEANLMKTL-QHDKLVRLYAVVT--R------EEPIYIITEYMAKGSLLDFLK---SD-------------------------------------------------------------------------------------------------EGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSE-----SLMCKIADFGLARVI----E--DNEY-TAREGAKFPIKWTAPEAIN-FGCFTIKSDVWSFGILLYEIVTYGKIP---------------YPGR-TNADVMTALSQGYRMPRVENCP------------------------------DELYDIMKMCWKEKAEERPTFDYLQSV--L--
---YQNLSPVGSGAYGSVCAAFDT-------KTGLRVAVKKLSRPFQSIIH---AKRTYRELRLLKHM-KHENVIGLLDVFT--PARSLEEFNDVYLVTHLMGA-DLNNIVK---CQ----------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNE-----DCELKILDFGLAR-------HTDDEM-TG---YVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLT-GRTL---------------FPGT-DHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYF--
--I-VLKRELGEGAFGKVFLAECYN--LCPEQDKILVAVKTLK-DASD-NA--RKDFH-REAELLTNL-QHEHIVKFYGVCV--E------GDPLIMVFEYMKHGDLNKFLR---AHGPDAV--------------------------------------------------------------------------------------LMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGE-----NLLVKIGDFGMSRDV---YS--TDYY-RVGGHTMLPIRWMPPESIM-YRKFTTESDVWSLGVVLWEIFTYGKQP---------------WYQL-SNNEVIECITQGRVLQRPRTCP------------------------------QEVYELMLGCWQREPHMRKNIKGIHTL--L--
--L-VLGRVLGSGAFGKVVEGTAYG--LSRSQPVMKVAVKMLK-PTAR-SSE-KQALM-SELKIMTHLGPHLNIVNLLGACT--K------SGPIYIITEYCFYGDLVNYLH---KNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQA-DTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQ-----GKIVKICDFGLARDI---MH--DSNY-VSKGSTFLPVKWMAPESIF-DNLYTTLSDVWSYGILLWEIFSLGGTP---------------YPGMMVDSTFYNKIKSGYRMAKPDHAT------------------------------SEVYEIMVKCWNSEPEKRPSFYHLSE---IV-
--L-VLGRTLGSGAFGQVVEATAHG--LSHSQATMKVAVKMLK-STAR-SSE-KQALM-SELKIMSHLGPHLNVVNLLGACT--K------GGPIYIITEYCRYGDLVDYLH---RNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICE-----GKLVKICDFGLARDI---MR--DSNY-ISKGSTFLPLKWMAPESIF-NSLYTTLSDVWSFGILLWEIFTLGGTP---------------YPELPMNEQFYNAIKRGYRMAQPAHAS------------------------------DEIYEIMQKCWEEKFEIRPPFSQLVL---LL-
--L-VLGKTLGEGEFGKVVKATAFH--LKGRAGYTTVAVKMLK-ENAS-PSE-LRDLL-SEFNVLKQV-NHPHVIKLYGACS--Q------DGPLLLIVEYAKYGSLRGFLR---ESRKVGPGYLGSG---------------------------------------------------------------------------GSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAE-----GRKMKISDFGLSRDV---YE--EDSY-VKRSQGRIPVKWMAIESLF-DHIYTTQSDVWSFGVLLWEIVTLGGNP---------------YPGI-PPERLFNLLKTGHRMERPDNCS------------------------------EEMYRLMLQCWKQEPDKRPVFADISKDL----
--L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-PGTM-SP---EAFL-QEAQVMKKL-RHEKLVQLYAVVS---------EEPIYIVTEYMSKGSLLDFLK---GE-------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGE-----NLVCKVADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILLTELTTKGRVP---------------YPGM-VNREVLDQVERGYRMPCPPECP------------------------------ESLHDLMCQCWRKEPEERPTFEYLQAF--L--
TLE---DKELGSGNFGTVKKGYYQ-----MKKVVKTVAVKILK-NEAN-DPALKDELL-AEANVMQQL-DNPYIVRMIGICE---------AESWMLVMEMAELGPLNKYLQ---QN---------------------------------------------------------------------------------------------------RHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVT-----QHYAKISDFGLSKAL---RA--DENYYKAQTHGKWPVKWYAPECIN-YYKFSSKSDVWSFGVLMWEAFSYGQKP---------------YRGM-KGSEVTAMLEKGERMGCPAGCP------------------------------REMYDLMNLCWTYDVENRPGFAAVE----LRL
--L-RLEVKLGQGCFGEVWMGTWN--------GTTKVAIKTLK-PGTM-MP---EAFL-QEAQIMKKL-RHDKLVPLYAVVS---------EEPIYIVTEFMSKGSLLDFLK---EG-------------------------------------------------------------------------------------------------DGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGE-----NLVCKIADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILQTELVTKGRVP---------------YPGM-VNREVLEQVERGYRMPCPQGCP------------------------------ESLHELMNLCWKKDPDERPTFEYIQSFL----
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