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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK ROS1 203
TK ALK 1116
Other AURKA 133
Other AURKB 77
Other AURKC 43
TKL BRAF 456
CMGC CDK2 4
TK CSK 195
TK FER 563
TK FGFR2 481
TK FYN 271
AGC GRK6 186
TK HCK 241
TK JAK1 875
TK JAK2 849
TK JAK3 822
TK LTK 510
TKL MAP3K7 36
CAMK MARK2 53
CAMK MARK4 59
TK NTRK2 538
TK NTRK3 538
CAMK NUAK1 55
AGC PDPK1 82
AGC PKN1 615
AGC PRKCA 339
TK RET 724
TKL RIPK2 18
CAMK STK17A 61
TK TNK2 150
-LT--LRLLLGSGAFGEVYEGTAVDI-LGVGSGEIKVAVKTLK-K--G-ST-DQEKIE-FL-K-EAHLMSKF--N---HPNILKQLGVCL--LNEP--QYIILELMEGGDLLTYLR---KARM-----------------ATFYG-PLL---TLVDLVDLCVDISKGCVYLERM--H---FIHRDLAARNCLVSVKDYTSPR-IV---KIGDFGLARDI---Y---K--NDYY--------------RKRGEGLLPVRWMAPESLM---DGI---FTTQSDVWSFGILIWEILTLGHQP---------------YPAH---SNLDVLNYV-QTGGR----LEPPRNCP----------------------------DDLWNLMTQCWAQEPDQRPT-----FHRIQDQL----------------
--I-TLIRGLGHGAFGEVYEGQVSG--MPNDPSPLQVAVKTLP-E--V-CS-EQDELD-FL-M-EALIISKF--N---HQNIVRCIGVSL--QSLP--RFILLELMAGGDLKSFLR---ETRP-----------------RPSQP-SSL---AMLDLLHVARDIACGCQYLEEN--H---FIHRDIAARNCLLTCP--GPGR-VA---KIGDFGMARDI---Y---R--ASYY--------------RKGGCAMLPVKWMPPEAFM---EGI---FTSKTDTWSFGVLLWEIFSLGYMP---------------YPSK---SNQEVLEFV-TSGGR----MDPPKNCP----------------------------GPVYRIMTQCWQHQPEDRPN-----FAIILER--I--------------
---FEIGRPLGKGKFGNVYLAREK-------QSKFILALKVLFKA--QLEKAGV--EH-QLRR-EVEIQSHL--R---HPNILRLYGYFH--DATR--VYLILEYAPLGTVYRELQ--KLS--------------------------KF---DEQRTATYITELANALSYCHSK--R---VIHRDIKPENLLLGS-----AG-EL---KIADFGWSV---------HAPSSRR--------------TT---LCGTLDYLPPEMIE---GRM---HDEKVDLWSLGVLCYEFLV-GKPP---------------FEAN------TYQETY-KRISR----VEFTFPDFVT--------------------------EGARDLISRLLKHNPSQRPM-----LREVLEHPWI--------------
---FEIGRPLGKGKFGNVYLAREK-------KSHFIVALKVLFKS--Q-IEKEG--VE-HQLRREIEIQAHL--H---HPNILRLYNYFY--DRRR--IYLILEYAPRGELYKELQ--KSC--------------------------TF---DEQRTATIMEELADALMYCHGK--K---VIHRDIKPENLLLGL-----KG-EL---KIADFGWSV---------HAPSLRR--------------KT---MCGTLDYLPPEMIE---GRM---HNEKVDLWCIGVLCYELLV-GNPP---------------FESA------SHNETY-RRIVK----VDLKFPASVP--------------------------MGAQDLISKLLRHNPSERLP-----LAQVSAHPWV--------------
---FEIGRPLGKGKFGNVYLARLK-------ESHFIVALKVLFKS--Q-IEKEG--LE-HQLRREIEIQAHL--Q---HPNILRLYNYFH--DARR--VYLILEYAPRGELYKELQ--KSE--------------------------KL---DEQRTATIIEELADALTYCHDK--K---VIHRDIKPENLLLGF-----RG-EV---KIADFGWSV---------HTPSLRR--------------KT---MCGTLDYLPPEMIE---GRT---YDEKVDLWCIGVLCYELLV-GYPP---------------FESA------SHSETY-RRILK----VDVRFPLSMP--------------------------LGARDLISRLLRYQPLERLP-----LAQILKHPWV--------------
---ITVGQRIGSGSFGTVYKGKWH----------GDVAVKMLN-V-TA-PT-PQQLQA-FK-N-EVGVLRKT--R---HVNILLFMGYST---KPQ--LAIVTQWCEGSSLYHHLH---II-----------------------E-TKF---EMIKLIDIARQTAQGMDYLHAK--S---IIHRDLKSNNIFLHE-----DL-TV---KIGDFGLATVK---S---R--WSGS--------------HQFEQLSGSILWMAPEVIR---MQDKNPYSFQSDVYAFGIVLYELMT-GQLP---------------YSNI-N-NRDQIIFMV-GRGYLSPDLSKVRSNCP----------------------------KAMKRLMAECLKKKRDERPL-----FPQILA---SI-------------
---FQKVEKIGEGTYGVVYKARNK-------LTGEVVALKKIRLD--T-ETEGV--PS-TAIR-EISLLKEL--N---HPNIVKLLDVIH--TENK--LYLVFEFLHQ-DLKKFMDASALT--------------------------GI---PLPLIKSYLFQLLQGLAFCHSH--R---VLHRDLKPQNLLINT-----EG-AI---KLADFGLARAF-------GVPVRTY--------------TH---EVVTLWYRAPEILL--GCKY---YSTAVDIWSLGCIFAEMVT-RRAL---------------FPGD---SEIDQLFRI-FRTLG----TPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-----AKAALAHPFF--------------
---LKLLQTIGKGEFGDVMLGDYR---------GNKVAVKCIK-N--D-AT-AQ---A-FL-A-EASVMTQL--R---HSNLVQLLGVIV-EEKGG--LYIVTEYMAKGSLVDYLR---SR----------------------GR-SVL---GGDCLLKFSLDVCEAMEYLEGN--N---FVHRDLAARNVLVSE-----DN-VA---KVSDFGLTKEA-------------S--------------STQDTGKLPVKWTAPEALR---EKK---FSTKSDVWSFGILLWEIYSFGRVP---------------YPRI---PLKDVVPRV-EKGYK----MDAPDGCP----------------------------PAVYEVMKNCWHLDAAMRPS-----FLQLREQL----------------
-VI--LGELLGKGNFGEVYKGTLK--------DKTSVAVKTCK-E--D-LP-QELKIK-FL-Q-EAKILKQY--D---HPNIVKLIGVCT--QRQP--VYIIMELVSGGDFLTFLR---RK-----------------------K-DEL---KLKQLVKFSLDAAAGMLYLESK--N---CIHRDLAARNCLVGE-----NN-VL---KISDFGMSRQE-------D--GGVY--------------SSSGLKQIPIKWTAPEALN---YGR---YSSESDVWSFGILLWETFSLGVCP---------------YPGM---TNQQAREQV-ERGYR----MSAPQHCP----------------------------EDISKIMMKCWDYKPENRPK-----FSELQKEL----------------
---LTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLK-D--D-AT-EKDLSD-LV-S-EMEMMKMIG-K---HKNIINLLGACT--QDGP--LYVIVEYASKGNLREYLR---ARRPPGMEY--------SYDINRVPE-EQM---TFKDLVSCTYQLARGMEYLASQ--K---CIHRDLAARNVLVTE-----NN-VM---KIADFGLARDI---N---N--IDYY--------------KKTTNGRLPVKWMAPEALF---DRV---YTHQSDVWSFGVLMWEIFTLGGSP---------------YPGI---PVEELFKLL-KEGHR----MDKPANCT----------------------------NELYMMMRDCWHAVPSQRPT-----FKQLVEDL----------------
---LQLIKRLGNGQFGEVWMGTWN--------GNTKVAIKTLK-P--G-TM-SP--ES-FL-E-EAQIMKKL--K---HDKLVQLYAVVS---EEP--IYIVTEYMNKGSLLDFLK---DG----------------------EG-RAL---KLPNLVDMAAQVAAGMAYIERM--N---YIHRDLRSANILVGN-----GL-IC---KIADFGLARLI-------E--DNEY--------------TARQGAKFPIKWTAPEAAL---YGR---FTIKSDVWSFGILLTELVTKGRVP---------------YPGM---NNREVLEQV-ERGYR----MPCPQDCP----------------------------ISLHELMIHCWKKDPEERPT-----FEYLQSFL----------------
--F-RQYRVLGKGGFGEVCACQVR-------ATGKMYACKKLEKK--R-IKKRK--GE-AMALNEKQILEKV--N---SRFVVSLAYAYE--TKDA--LCLVLTLMNGGDLKFHIYHMGQA--------------------------GF---PEARAVFYAAEICCGLEDLHRE--R---IVYRDLKPENILLDD-----HG-HI---RISDLGLAVH--------VPEGQTI--------------KG---RVGTVGYMAPEVVK---NER---YTFSPDWWALGCLLYEMIA-GQSP---------------FQQR---KKKIKREEV-ERLVK-----EVPEEYSER--FS----------------------PQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLF--------------
---LKLEKKLGAGQFGEVWMATYN--------KHTKVAVKTMK-P--G-SM-SV--EA-FL-A-EANVMKTL--Q---HDKLVKLHAVVT---KEP--IYIITEFMAKGSLLDFLK---SD----------------------EG-SKQ---PLPKLIDFSAQIAEGMAFIEQR--N---YIHRDLRAANILVSA-----SL-VC---KIADFGLARVI-------E--DNEY--------------TAREGAKFPIKWTAPEAIN---FGS---FTIKSDVWSFGILLMEIVTYGRIP---------------YPGM---SNPEVIRAL-ERGYR----MPRPENCP----------------------------EELYNIMMRCWKNRPEERPT-----FEYIQSV--L--------------
--L-KRIRDLGEGHFGKVELCRYDP---EGDNTGEQVAVKSLK-P--E-SG-GNHIAD-LK-K-EIEILRNL--Y---HENIVKYKGICT-ED-GGNGIKLIMEFLPSGSLKEYLP---KN-----------------------K-NKI---NLKQQLKYAVQICKGMDYLGSR--Q---YVHRDLAARNVLVES-----EH-QV---KIGDFGLTKAI--ET---D--KEYY--------------TVKDDRDSPVFWYAPECLM---QSK---FYIASDVWSFGVTLHELLTYCDSDSSPMALF-LKMIGPTHGQM---TVTRLVNTL-KEGKR----LPCPPNCP----------------------------DEVYQLMRKCWEFQPSNRTS-----FQNLIE---GF-------------
---LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-H--S-TE--EHLRD-FE-R-EIEILKSL--Q---HDNIVKYKGVCYSAGRRN--LKLIMEYLPYGSLRDYLQ---KH-----------------------K-ERI---DHIKLLQYTSQICKGMEYLGTK--R---YIHRDLATRNILVEN-----EN-RV---KIGDFGLTKVL--PQ---D--KEYY--------------KVKEPGESPIFWYAPESLT---ESK---FSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM---IVFHLIELL-KNNGR----LPRPDGCP----------------------------DEIYMIMTECWNNNVNQRPS-----FRDLA----LRV------------
--L-KYISQLGKGNFGSVELCRYDP---LGDNTGALVAVKQLQ-H--S-GP-DQ-QRD-FQ-R-EIQILKAL--H---SDFIVKYRGVSYGPGRQS--LRLVMEYLPSGCLRDFLQ---RH-----------------------R-ARL---DASRLLLYSSQICKGMEYLGSR--R---CVHRDLAARNILVES-----EA-HV---KIADFGLAKLL--PL---D--KDYY--------------VVREPGQSPIFWYAPESLS---DNI---FSRQSDVWSFGVVLYELFTYCDKSCSPSAE--FLRMMGCERDV-P-ALCRLLELL-EEGQR----LPAPPACP----------------------------AEVHELMKLCWAPSPQDRPS-----FSALGP---QL-------------
--V-TLLRALGHGAFGEVYEGLVIG--LPGDSSPLQVAIKTLP-E--L-CS-PQDELD-FL-M-EALIISKF--R---HQNIVRCVGLSL--RATP--RLILLELMSGGDMKSFLR---HSRP-----------------HLGQP-SPL---VMRDLLQLAQDIAQGCHYLEEN--H---FIHRDIAARNCLLSCA--GPSR-VA---KIGDFGMARDI---Y---R--ASYY--------------RRGDRALLPVKWMPPEAFL---EGI---FTSKTDSWSFGVLLWEIFSLGYMP---------------YPGR---TNQEVLDFV-VGGGR----MDPPRGCP----------------------------GPVYRIMTQCWQHEPELRPS-----FASILERL----------------
---IEVEEVVGRGAFGVVCKAKWR---------AKDVAIKQIE-------S-ESERKA-FI-V-ELRQLSRV--N---HPNIVKLYGACL----NP--VCLVMEYAEGGSLYNVLH---GA----------------------EPLPYY---TAAHAMSWCLQCSQGVAYLHSM--QPKALIHRDLKPPNLLLVA-----GGTVL---KICDFGTACDI-------------Q--------------THMTNNKGSAAWMAPEVFE---GSN---YSEKCDVFSWGIILWEVITR-RKP---------------FDEIGG-PAFRIMWAV-HNGTR----PPLIKNLP----------------------------KPIESLMTRCWSKDPSQRPS-----MEEIVKI--M--------------
---YRLLKTIGKGNFAKVKLARHI-------LTGKEVAVKIIDKT--Q-LNSSS--LQ-KLFR-EVRIMKVL--N---HPNIVKLFEVIE--TEKT--LYLVMEYASGGEVFDYLV--AHG--------------------------RM---KEKEARAKFRQIVSAVQYCHQK--F---IVHRDLKAENLLLDA-----DM-NI---KIADFGFSNE--------FTFGNKL--------------DT---FCGSPPYAAPELFQ--GKKY---DGPEVDVWSLGVILYTLVS-GSLP---------------FDGQ------NLKELR-ERVLR----GKYRIPFYMS--------------------------TDCENLLKKFLILNPSKRGT-----LEQIMKDRWM--------------
---YRLLRTIGKGNFAKVKLARHI-------LTGREVAIKIIDKT--Q-LNPSS--LQ-KLFR-EVRIMKGL--N---HPNIVKLFEVIE--TEKT--LYLVMEYASAGEVFDYLV--SHG--------------------------RM---KEKEARAKFRQIVSAVHYCHQK--N---IVHRDLKAENLLLDA-----EA-NI---KIADFGFSNE--------FTLGSKL--------------DT---FCGSPPYAAPELFQ--GKKY---DGPEVDIWSLGVILYTLVS-GSLP---------------FDGH------NLKELR-ERVLR----GKYRVPFYMS--------------------------TDCESILRRFLVLNPAKRCT-----LEQIMKDKWI--------------
--I-VLKRELGEGAFGKVFLAECYN--LCPEQDKILVAVKTLK-D--A-SD-NA-RKD-FH-R-EAELLTNL--Q---HEHIVKFYGVCV--EGDP--LIMVFEYMKHGDLNKFLR---AHGPDAV-----------LMAEGNPP-TEL---TQSQMLHIAQQIAAGMVYLASQ--H---FVHRDLATRNCLVGE-----NL-LV---KIGDFGMSRDV---Y---S--TDYY--------------RVGGHTMLPIRWMPPESIM---YRK---FTTESDVWSLGVVLWEIFTYGKQP---------------WYQL---SNNEVIECI-TQGRV----LQRPRTCP----------------------------QEVYELMLGCWQREPHMRKN-----IKGIHTL--L--------------
--I-VLKRELGEGAFGKVFLAECYN--LSPTKDKMLVAVKALK-D--P-TL-AA-RKD-FQ-R-EAELLTNL--Q---HEHIVKFYGVCG--DGDP--LIMVFEYMKHGDLNKFLR---AHGPDAMIL--------VDGQPRQAK-GEL---GLSQMLHIASQIASGMVYLASQ--H---FVHRDLATRNCLVGA-----NL-LV---KIGDFGMSRDV---Y---S--TDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIM---YRK---FTTESDVWSFGVILWEIFTYGKQP---------------WFQL---SNTEVIECI-TQGRV----LERPRVCP----------------------------KEVYDVMLGCWQREPQQRLN-----IKEIYKIL----------------
---YELQETLGKGTYGKVKRATER-------FSGRVVAIKSIRKD--K-IKDEQ--DM-VHIRREIEIMSSL--N---HPHIISIYEVFE--NKDK--IVIIMEYASKGELYDYIS--ERR--------------------------RL---SERETRHFFRQIVSAVHYCHKN--G---VVHRDLKLENILLDD-----NC-NI---KIADFGLSNL--------YQKDKFL--------------QT---FCGSPLYASPEIVN--GRPY---RGPEVDSWALGVLLYTLVY-GTMP---------------FDGF------DHKNLI-RQISS----GEYREPTQP---------------------------SDARGLIRWMLMVNPDRRAT-----IEDIANHWW-V-------------
--F-KFGKILGEGSFSTVVLAREL-------ATSREYAIKILEKR--H-IIKEN--KVPYVTR-ERDVMSRL--D---HPFFVKLYFTFQ--DDEK--LYFGLSYAKNGELLKYIR--KIG--------------------------SF---DETCTRFYTAEIVSALEYLHGK--G---IIHRDLKPENILLNE-----DM-HI---QITDFGTAKVLS-----PESKQARA--------------NS---FVGTAQYVSPELLT---EKS---ACKSSDLWALGCIIYQLVA-GLPP---------------FRAG------NEYLIF-QKIIK----LEYDFPEKFF--------------------------PKARDLVEKLLVLDATKRLG-----CEEM------EGYGPLKAHPF---
--F-KFLAVLGRGHFGKVLLSEFR-------PSGELFAIKALKKG--D-IVARD--EV-ESLMCEKRILAAV--TSAGHPFLVNLFGCFQ--TPEH--VCFVMEYSAGGDLMLHIH---SD--------------------------VF---SEPRAIFYSACVVLGLQFLHEH--K---IVYRDLKLDNLLLDT-----EG-YV---KIADFGLCKEG-------MGYGDRT--------------ST---FCGTPEFLAPEVLT---DTS---YTRAVDWWGLGVLLYEMLV-GESP---------------FPGD------DEEEVF-DSIVN----DEVRYPRFLS--------------------------AEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFF--------------
--F-NFLMVLGKGSFGKVMLADRK-------GTEELYAIKILKKD--V-VIQDD--DV-ECTMVEKRVLALL-DK---PPFLTQLHSCFQ--TVDR--LYFVMEYVNGGDLMYHIQ--QVG--------------------------KF---KEPQAVFYAAEISIGLFFLHKR--G---IIYRDLKLDNVMLDS-----EG-HI---KIADFGMCKEH-------MMDGVTT--------------RT---FCGTPDYIAPEIIA---YQP---YGKSVDWWAYGVLLYEMLA-GQPP---------------FDGE------DEDELF-QSIME----HNVSYPKSLS--------------------------KEAVSVCKGLMTKHPAKRLG-----C---------GPEGERDVREHAFF
--L-VLGKTLGEGEFGKVVKATAFH--LKGRAGYTTVAVKMLK-E--N-AS-PSELRD-LL-S-EFNVLKQV--N---HPHVIKLYGACS--QDGP--LLLIVEYAKYGSLRGFLR---ESRKVGPGYLGSGGSRNSSSLDHPDE-RAL---TMGDLISFAWQISQGMQYLAEM--K---LVHRDLAARNILVAE-----GR-KM---KISDFGLSRDV---Y---E--EDSY--------------VKRSQGRIPVKWMAIESLF---DHI---YTTQSDVWSFGVLLWEIVTLGGNP---------------YPGI---PPERLFNLL-KTGHR----MERPDNCS----------------------------EEMYRLMLQCWKQEPDKRPV-----FADISKDL----------------
LAD---LRYLSRGASGTVSSARHA-------DWRVQVAVKHLHIH--T-PLLDS--ER-KDVLREAEILHKA--R---FSYILPILGICN--EPEF--LGIVTEYMPNGSLNELLH--RKT--------------------------EYPDVAWPLRFRILHEIALGVNYLHNMTPP---LLHHDLKTQNILLDN-----EF-HV---KIADFGLSKWRMM--SLSQSRSSKS--------------AP---EGGTIIYMPPENYEPGQKSR---ASIKHDIYSYAVITWEVLS-RKQP---------------FEDVT-NPLQIMYSVS-QGHRP----VINEESLPYDIPHR----------------------ARMISLIESGWAQNPDERPS-----FLKCL-----IEL-----------
LCP---GRELGRGKFAVVRKCIKK-------DSGKEFAAKFMRKR--R-KGQDC--RM-EIIH-EIAVLELA-QD---NPWVINLHEVYE--TASE--MILVLEYAAGGEIFDQCVADREE--------------------------AF---KEKDVQRLMRQILEGVHFLHTR--D---VVHLDLKPQNILLTS-----ES-PLGDIKIVDFGLSRI--------LKNSEEL--------------RE---IMGTPEYVAPEILS---YDP---ISMATDMWSIGVLTYVMLT-GISP---------------FLGN------DKQETF-LNISQ----MNLSYSEEEFDVLS----------------------ESAVDFIRTLLVKKPEDRAT-----AEECLKHPWL--------------
--L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVL-SQ-PEAMDD-FI-R-EVNAMHSL--D---HRNLIRLYGVVL---TPP--MKMVTELAPLGSLLDRLR---KH-----------------------Q-GHF---LLGTLSRYAVQVAEGMGYLESK--R---FIHRDLAARNLLLAT-----RD-LV---KIGDFGLMRAL--PQ---N--DDHY--------------VMQEHRKVPFAWCAPESLK---TRT---FSHASDTWMFGVTLWEMFTYGQEP---------------WIGL---NGSQILHKIDKEGER----LPRPEDCP----------------------------QDIYNVMVQCWAHKPEDRPT-----FVALRDF--L--------------
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