Microbial hydantoinase (HYD) is an industrial enzyme.
This enzyme has been used for the commercial production
of optically pure D- or L-active amino acids in biocatalysis.
It has also been used for the production of D-amino acid
intermediate compounds through stereospecific hydrolysis of
chemically synthesized cyclic hydantoins. These intermediates
are widely used for semisynthetic antibiotics, peptide hormones,
pyrethroids, and pesticides
|Figure 1. The production of D-amino acid. D-hydantoinase (red circle) serves as an important enzyme in the pathway.
On our research cooperating with
Yuh-Shyong Yang, we have identified a new substrate for D-HYD by GEMDOCK.
Figure 2 showed the docked conformation of parabanate in D-HYD. Because the docked
orientation was correspondent with the proposed conformation of the known substrate.
Therefore we deduced that parabanate was a substrate for D-HYD. The experimental
result also proved the hypothesis.
|Figure 2. The docked conformation of parabanate.