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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK ABL1 203
TK ALK 1116
Other AURKA 133
CMGC CDK2 4
CAMK DAPK1 13
TK EGFR 712
TK EPHA3 621
TK ERBB3 709
TK FGFR1 478
TK FGFR2 481
TK HCK 241
TK IGF1R 999
TK INSR 1023
TKL IRAK4 186
TK KIT 589
TK LCK 245
STE MAP2K1 68
TK MET 1078
Other NEK2 8
STE OXSR1 17
AGC PDPK1 82
Other PLK4 12
TK PTK2B 425
AGC RPS6KA1 62
TK SRC 270
STE STK24 24
TK TNK2 150
TK ZAP70 338
----ITMKHKLGGGQYGEV---YEGVWK-------KYSLTVAVKTLK-E--D-TM-EV--E-E-FL-K----EAAVMKEI-KHPNLVQLLGVCT-R--EPPFYIITEFMTYGNLLDYLR---------EC--------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEK-K-----NFIHRDLAARNCLVGE---NHLV----KVADFGLSRLM-------T--GDTY--TAHAGAKFPIKWTAPESLA-YNKFSIKSDVWAFGVLLWEIATYGMSPYPGI-DLSQVYELL-EKDYRMERPEGCP-------------------------------------------EKVYELMRACWQWNPSDRPS-----FAEIHQA--F-----------
---I-TLIRGLGHGAFGEV---YEGQVSG--MPNDPSPLQVAVKTLP-E--V-CS-EQDEL-D-FL-M----EALIISKF-NHQNIVRCIGVSL-Q--SLPRFILLELMAGGDLKSFLR---------ETRP---------------------------------------------------------------------RPSQPSSLAMLDLLHVARDIACGCQYLEE-N-----HFIHRDIAARNCLLTCPGPGRVA----KIGDFGMARDI---Y---R--ASYY--RKGGCAMLPVKWMPPEAFM-EGIFTSKTDTWSFGVLLWEIFSLGYMPYPSK-SNQEVLEFV-TSGGRMDPPKNCP-------------------------------------------GPVYRIMTQCWQHQPEDRPN-----FAIILER--I-----------
----FEIGRPLGKGKFGNV---YLAREK-------QSKFILALKVLFKA--QLEKAGV--E-H-QLRR----EVEIQSHL-RHPNILRLYGYFH-D--ATRVYLILEYAPLGTVYRELQ--------KLS-----------------------------------------------------------------------------KFDEQRTATYITELANALSYCHS-K-----RVIHRDIKPENLLLGS---AGEL----KIADFGWSV---------HAPSSRR--TT---LCGTLDYLPPEMIE-GRMHDEKVDLWSLGVLCYEFLV-GKPPFEAN----TYQETY-KRISRVEFTFPDFVT-----------------------------------------EGARDLISRLLKHNPSQRPM-----LREVLEHPWI-----------
----FQKVEKIGEGTYGVV---YKARNK-------LTGEVVALKKIRLD--T-ETEGV--P-S-TAIR----EISLLKEL-NHPNIVKLLDVIH-T--ENKLYLVFEFLHQ-DLKKFMDA------SALT-----------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHS-H-----RVLHRDLKPQNLLINT---EGAI----KLADFGLARAF-------GVPVRTY--TH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-RRALFPGD-SEIDQLFRI-FRTLGTPDEVVWPGVTSMPDYKPSFPKWAR---------------QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-----AKAALAHPFF-----------
----YDTGEELGSGQFAVV---KKCREK-------STGLQYAAKFIKKR--R-TKSSR--R-G-VSREDIEREVSILKEI-QHPNVITLHEVYE-N--KTDVILILELVAGGELFDFLA--------EKE-----------------------------------------------------------------------------SLTEEEATEFLKQILNGVYYLHS-L-----QIAHFDLKPENIMLLD---RNVPKPRIKIIDFGLAHK--------IDFGNEF--KN---IFGTPEFVAPEIVN-YEPLGLEADMWSIGVITYILLS-GASPFLGD----TKQETL-ANVSAVNYEFEDEYFSNTS-------------------------------------ALAKDFIRRLLVKDPKKRMT-----IQDSLQHPWI-----------
---F-KKIKVLGSGAFGTV---YKGLWIP---EGEKVKIPVAIKELR-E--A-TS-PKANK-E-IL-D----EAYVMASV-DNPHVCRLLGICL----TSTVQLITQLMPFGCLLDYVR---------EH---------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLED-R-----RLVHRDLAARNVLVKT---PQHV----KITDFGLAKLL---G---A--EEKE--YHAEGGKVPIKWMALESIL-HRIYTHQSDVWSYGVTVWELMTFGSKPYDGI-PASEISSIL-EKGERLPQPPICT-------------------------------------------IDVYMIMVKCWMIDADSRPK-----FRELI----IEF---------
---I-SIDKVVGAGEFGEV---CSGRLK----LPSKKEISVAIKTLK-V--G-YT-EKQRR-D-FL-G----EASIMGQF-DHPNIIRLEGVVT-K--SKPVMIVTEYMENGSLDSFLR---------KH---------------------------------------------------------------------------DAQFTVIQLVGMLRGIASGMKYLSD-M-----GYVHRDLAARNILINS---NLVC----KVSDFGLSRVL---E---D--DPEA-AYTTRGGKIPIRWTSPEAIA-YRKFTSASDVWSYGIVLWEVMSYGERPYWEM-SNQDVIKAV-DEGYRLPPPMDCP-------------------------------------------AALYQLMLDCWQKDRNNRPK-----FEQIVSIL-------------
---L-RKLKVLGSGVFGTV---HKGVWIP---EGESIKIPVCIKVIE-D--K-SG-RQSFQ-A-VT-D----HMLAIGSL-DHAHIVRLLGLCP----GSSLQLVTQYLPLGSLLDHVR---------QH---------------------------------------------------------------------------RGALGPQLLLNWGVQIAKGMYYLEE-H-----GMVHRNLAARNVLLKS---PSQV----QVADFGVADLL---P---P--DDKQ--LLYSEAKTPIKWMALESIH-FGKYTHQSDVWSYGVTVWELMTFGAEPYAGL-RLAEVPDLL-EKGERLAQPQICT-------------------------------------------IDVYMVMVKCWMIDENIRPT-----FKELANE--F-----------
---L-VLGKPLGEGCFGQV---VLAEAIGLDKDKPNRVTKVAVKMLK-S--D-AT-EKDLS-D-LI-S----EMEMMKMIGKHKNIINLLGACT-Q--DGPLYVIVEYASKGNLREYLQ---------ARRPPGLEY------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLAS-K-----KCIHRDLAARNVLVTE---DNVM----KIADFGLARDI---H---H--IDYY--KKTTNGRLPVKWMAPEALF-DRIYTHQSDVWSFGVLLWEIFTLGGSPYPGV-PVEELFKLL-KEGHRMDKPSNCT-------------------------------------------NELYMMMRDCWHAVPSQRPT-----FKQLVEDL-------------
----LTLGKPLGEGCFGQV---VMAEAVGIDKDKPKEAVTVAVKMLK-D--D-AT-EKDLS-D-LV-S----EMEMMKMIGKHKNIINLLGACT-Q--DGPLYVIVEYASKGNLREYLR---------ARRPPGMEY------------------------------------------------------------SYDINRVPEEQMTFKDLVSCTYQLARGMEYLAS-Q-----KCIHRDLAARNVLVTE---NNVM----KIADFGLARDI---N---N--IDYY--KKTTNGRLPVKWMAPEALF-DRVYTHQSDVWSFGVLMWEIFTLGGSPYPGI-PVEELFKLL-KEGHRMDKPANCT-------------------------------------------NELYMMMRDCWHAVPSQRPT-----FKQLVEDL-------------
----LKLEKKLGAGQFGEV---WMATYN--------KHTKVAVKTMK-P--G-SM-SV--E-A-FL-A----EANVMKTL-QHDKLVKLHAVVT----KEPIYIITEFMAKGSLLDFLK---------SD--------------------------------------------------------------------------EGSKQPLPKLIDFSAQIAEGMAFIEQ-R-----NYIHRDLRAANILVSA---SLVC----KIADFGLARVI-------E--DNEY--TAREGAKFPIKWTAPEAIN-FGSFTIKSDVWSFGILLMEIVTYGRIPYPGM-SNPEVIRAL-ERGYRMPRPENCP-------------------------------------------EELYNIMMRCWKNRPEERPT-----FEYIQSV--L-----------
----ITMSRELGQGSFGMV---YEGVAKG--VVKDEPETRVAIKTVN-E--A-AS-MRERI-E-FL-N----EASVMKEF-NCHHVVRLLGVVS-Q--GQPTLVIMELMTRGDLKSYLR---------SLRPEM------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNA-N-----KFVHRDLAARNCMVAE---DFTV----KIGDFGMTRDI---Y---E--TDYY--RKGGKGLLPVRWMSPESLK-DGVFTTYSDVWSFGVVLWEIATLAEQPYQGL-SNEQVLRFV-MEGGLLDKPDNCP-------------------------------------------DMLFELMRMCWQYNPKMRPS-----FLEIISS--I-----------
----ITLLRELGQGSFGMV---YEGNARD--IIKGEAETRVAVKTVN-E--S-AS-LRERI-E-FL-N----EASVMKGF-TCHHVVRLLGVVS-K--GQPTLVVMELMAHGDLKSYLR---------SLRPEA------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNA-K-----KFVHRDLAARNCMVAH---DFTV----KIGDFGMTRDI---Y---E--TDYY--RKGGKGLLPVRWMAPESLK-DGVFTTSSDMWSFGVVLWEITSLAEQPYQGL-SNEQVLKFV-MDGGYLDQPDNCP-------------------------------------------ERVTDLMRMCWQFNPKMRPT-----FLEIVNLL-------------
-VGG----NKMGEGGFGVV---YKGYVN-------NTT--VAVKKLAAM--V-DITTE--ELK-QQFD---QEIKVMAKC-QHENLVELLGFSS-D--GDDLCLVYVYMPNGSLLDRLSC-----LDGTP-----------------------------------------------------------------------------PLSWHMRCKIAQGAANGINFLHE-N-----HHIHRDIKSANILLDE---AFTA----KISDFGLARASE-----KFAQTVMT--SR---IVGTTAYMAPEALR--GEITPKSDIYSFGVVLLEIIT-GLPAVDEH-REPQLLLDI-KEEIEDEEKTIEDYIDKKMNDAD-----------------------------STSVEAMYSVASQCLHEKKNKRPD-----IKKVQQ----LLQ--------
----LSFGKTLGAGAFGKV---VEATAYG--LIKSDAAMTVAVKMLK-P--S-AH-LTERE-A-LM-S----ELKVLSYLGNHMNIVNLLGACT-I--GGPTLVITEYCCYGDLLNFLR---------RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLAS-K-----NCIHRDLAARNILLTH---GRIT----KICDFGLARDI---K---N--DSNY--VVKGNARLPVKWMAPESIF-NCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMI-KEGFRMLSPEHAP-------------------------------------------AEMYDIMKTCWDADPLKRPT-----FKQIVQL--I-----------
----LKLVERLGAGQFGEV---WMGYYN--------GHTKVAVKSLK-Q--G-SM-SP--D-A-FL-A----EANLMKQL-QHQRLVRLYAVVT----QEPIYIITEYMENGSLVDFLK---------TP--------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEE-R-----NYIHRDLRAANILVSD---TLSC----KIADFGLARLI-------E--DNEY--TAREGAKFPIKWTAPEAIN-YGTFTIKSDVWSFGILLTEIVTHGRIPYPGM-TNPEVIQNL-ERGYRMVRPDNCP-------------------------------------------EELYQLMRLCWKERPEDRPT-----FDYLRSV--L-----------
----FEKISELGAGNGGVV---FKVSHK-------PSGL-VMARKLIHL--E-IKPAI--R-N-QIIR----ELQVLHEC-NSPYIVGFYGAFY-S--DGEISICMEHMDGGSLDQVLK--------KAG-----------------------------------------------------------------------------RIPEQILGKVSIAVIKGLTYLREKH-----KIMHRDVKPSNILVNS---RGEI----KLCDFGVSG---------QLIDSMA--NS---FVGTRSYMSPERLQ-GTHYSVQSDIWSMGLSLVEMAV-GRYPIPPP-DAKELELMF-GCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERAD-----LKQLMVHAFI-----------
----VHFNEVIGRGHFGCV---YHGTLL----DNDGKKIHCAVKSLN-R--I-TD-IGEVS-Q-FL-T----EGIIMKDF-SHPNVLSLLGICLRS--EGSPLVVLPYMKHGDLRNFIR---------NE---------------------------------------------------------------------------THNPTVKDLIGFGLQVAKGMKYLAS-K-----KFVHRDLAARNCMLDE---KFTV----KVADFGLARDM---Y---D--KEYYSVHNKTGAKLPVKWMALESLQ-TQKFTTKSDVWSFGVLLWELMTRGAPPYPDV-NTFDITVYL-LQGRRLLQPEYCP-------------------------------------------DPLYEVMLKCWHPKAEMRPS-----FSELVSR--I-----------
----YEVLYTIGTGSYGRC---QKIRRK-------SDGKILVWKELDYG--S-MTEAE--K-Q-MLVS----EVNLLREL-KHPNIVRYYDRII-DRTNTTLYIVMEYCEGGDLASVITKG----TKERQ-----------------------------------------------------------------------------YLDEEFVLRVMTQLTLALKECHR-RSDGGHTVLHRDLKPANVFLDG---KQNV----KLGDFGLARIL-------NHDTSFA--KT---FVGTPYYMSPEQMN-RMSYNEKSDIWSLGCLLYELCA-LMPPFTAF----SQKELA-GKIREGKFRRIPYRY---S-------------------------------------DELNEIITRMLNLKDYHRPS-----VEEILENPL-I----------
----YELQEVIGSGATAVV---QAAYCA-------PKKEKVAIKRINLE--K-CQTSM--D-E--LLK----EIQAMSQC-HHPNIVSYYTSFV-V--KDELWLVMKLLSGGSVLDIIKHIVAKGEHKSG-----------------------------------------------------------------------------VLDESTIATILREVLEGLEYLHK-N-----GQIHRDVKAGNILLGE---DGSV----QIADFGVSAFLAT--GGDITRNKVR--KT---FVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELAT-GAAPYHKY-PPMKVLMLT-LQNDPPSLETGVQDKE--MLK--------------------------------KYGKSFRKMISLCLQKDPEKRPT-----AAELLRHKFF-----------
---F-KFGKILGEGSFSTV---VLAREL-------ATSREYAIKILEKR--H-IIKEN--K-VPYVTR----ERDVMSRL-DHPFFVKLYFTFQ-D--DEKLYFGLSYAKNGELLKYIR--------KIG-----------------------------------------------------------------------------SFDETCTRFYTAEIVSALEYLHG-K-----GIIHRDLKPENILLNE---DMHI----QITDFGTAKVLS-----PESKQARA--NS---FVGTAQYVSPELLT-EKSACKSSDLWALGCIIYQLVA-GLPPFRAG----NEYLIF-QKIIKLEYDFPEKFF-----------------------------------------PKARDLVEKLLVLDATKRLG-----CEEM------EGYGPLKAHPF
---F-KVGNLLGKGSFAGV---YRAESI-------HTGLEVAIKMIDKK--A-MYKAG--M-V-QRVQ---NEVKIHCQL-KHPSILELYNYFE-D--SNYVYLVLEMCHNGEMNRYLK-------NRVK-----------------------------------------------------------------------------PFSENEARHFMHQIITGMLYLHS-H-----GILHRDLTLSNLLLTR---NMNI----KIADFGLATQL-------KMPHEKH--YT---LCGTPNYISPEIAT-RSAHGLESDVWSLGCMFYTLLI-GRPPFDTD----TVKNTL-NKVVLADYEMPSFLS-----------------------------------------IEAKDLIHQLLRRNPADRLS-----LSSVLDHPFM-----------
---V-VLNRILGEGFFGEV---YEGVYT----NHKGEKINVAVKTCK-K--D-CT-LDNKE-K-FM-S----EAVIMKNL-DHPHIVKLIGIIE----EEPTWIIMELYPYGELGHYLE---------RN---------------------------------------------------------------------------KNSLKVLTLVLYSLQICKAMAYLES-I-----NCVHRDIAVRNILVAS---PECV----KLGDFGLSRYI-------E--DEDY--YKASVTRLPIKWMSPESIN-FRRFTTASDVWMFAVCMWEILSFGKQPFFWL-ENKDVIGVL-EKGDRLPKPDLCP-------------------------------------------PVLYTLMTRCWDYDPSDRPR-----FTELVCS--L-----------
----FELLKVLGQGSFGKVFLVRKVTRP-------DSGHLYAMKVLKKA--T-LKVRD--R-V-RTKM----ERDILADV-NHPFVVKLHYAFQ-T--EGKLYLILDFLRGGDLFTRLS--------KEV-----------------------------------------------------------------------------MFTEEDVKFYLAELALGLDHLHS-L-----GIIYRDLKPENILLDE---EGHI----KLTDFGLSKEA-------IDHEKKA--YS---FCGTVEYMAPEVVN-RQGHSHSADWWSYGVLMFEMLT-GSLPFQGK----DRKETM-TLILKAKLGMPQFLS-----------------------------------------TEAQSLLRALFKRNPANRLGSGPDGAEEIKRHV--FY---------
---L-RLEVKLGQGCFGEV---WMGTWN--------GTTRVAIKTLK-P--G-TM-SP--E-A-FL-Q----EAQVMKKL-RHEKLVQLYAVVS----EEPIYIVTEYMSKGSLLDFLK---------GE--------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVER-M-----NYVHRDLRAANILVGE---NLVC----KVADFGLARLI-------E--DNEY--TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILLTELTTKGRVPYPGM-VNREVLDQV-ERGYRMPCPPECP-------------------------------------------ESLHDLMCQCWRKEPEERPT-----FEYLQAF--L-----------
----FTKLEKIGKGSFGEV---FKGIDN-------RTQKVVAIKIIDLE--E-AEDEI--E-D-IQ-Q----EITVLSQC-DSPYVTKYYGSYL-K--DTKLWIIMEYLGGGSALDLLE---------PG-----------------------------------------------------------------------------PLDETQIATILREILKGLDYLHS-E-----KKIHRDIKAANVLLSE---HGEV----KLADFGVAGQL-------TDTQIKR--NT---FVGTPFWMAPEVIK-QSAYDSKADIWSLGITAIELAR-GEPPHSEL-HPMKVLFLI-PKNNPPTLEGNYS-------------------------------------------KPLKEFVEACLNKEPSFRPT-----AKELLKHKFI-----------
---L-RLLEKLGDGSFGVV---RRGEWD----APSGKTVSVAVKCLK-PDVL-SQ-PEAMD-D-FI-R----EVNAMHSL-DHRNLIRLYGVVL----TPPMKMVTELAPLGSLLDRLR---------KH---------------------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLES-K-----RFIHRDLAARNLLLAT---RDLV----KIGDFGLMRAL--PQ---N--DDHY--VMQEHRKVPFAWCAPESLK-TRTFSHASDTWMFGVTLWEMFTYGQEPWIGL-NGSQILHKIDKEGERLPRPEDCP-------------------------------------------QDIYNVMVQCWAHKPEDRPT-----FVALRDF--L-----------
LIAD----IELGCGNFGSV---RQGVYR-----MRKKQIDVAIKVLK-Q--G-TE-KADTE-E-MM-R----EAQIMHQL-DNPYIVRLIGVCQ----AEALMLVMEMAGGGPLHKFLV---------GK---------------------------------------------------------------------------REEIPVSNVAELLHQVSMGMKYLEE-K-----NFVHRDLAARNVLLVN---RHYA----KISDFGLSKAL--GA---D--DSYY--TARSAGKWPLKWYAPECIN-FRKFSSRSDVWSYGVTMWEALSYGQKPYKKM-KGPEVMAFI-EQGKRMECPPECP-------------------------------------------PELYALMSDCWIYKWEDRPD-----FLTVEQR--M-----------
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