|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
CMGC CDK2 203
TK ABL1 261
Other AURKA 133
Other AURKB 77
TK BLK 241
CMGC CDC2 4
CMGC CDK4 6
CAMK CHEK1 9
CAMK CHEK2 220
TK FGR 263
TK FYN 271
TK HCK 241
TKL IRAK4 186
TK JAK2 849
TK LCK 245
STE MAP2K1 68
CMGC MAPK14 24
AGC PDPK1 82
AGC PRKCA 339
TK PTK2 422
TK PTK6 191
TK SRC 270
TK SRMS 230
TK TNK2 150
TK YES1 277
TK ZAP70 338
----FQKVEKIGEGTYGVVYKARNK----LTGEVVALKKIRLD--T-ETE---GV--P-S-TAIR------EISLLKEL-N---HPNIVKLLDVIH--T------ENKLYLVFEFLHQ-DLKKFMDA-SALT---GIPLPLIKSYLFQLLQGLAFCHS-HRVLHRDLKPQNLLINTEGAI---KLADFGLARAF----GVPVRTYTH---EVVTLWYRAPEILL--GCKYYSTAVDIWSLGCIFAEMVT-RRAL---------------FPGDSEIDQLFRI-FRTLGTPDEVVWPGVTSMPDYKPSFPKWAR---------------QDFSKVVPPLDEDGRS-LLSQMLHYDPNKRISAKAALAHPFF--------------
----ITMKHKLGGGQYGEVYEGVWK----KYSLTVAVKTLK-E--D-TM----EV--E-E-FL-K------EAAVMKEI-K---HPNLVQLLGVCT--R------EPPFYIITEFMTYGNLLDYLR----ECNRQEVNAVVLLYMATQISSAMEYLEK-KNFIHRDLAARNCLVGENHLV---KVADFGLSRLM----T--GDTYTAHAGAKFPIKWTAPESLA---YNKFSIKSDVWAFGVLLWEIATYGMSP---------------YPGIDLSQVYELL-EKDYRMERPEGCP-------------------------------------------EKVYE-LMRACWQWNPSDRPSFAEIHQA--F--------------
----FEIGRPLGKGKFGNVYLAREK----QSKFILALKVLFKA--QLEKA---GV--E-H-QLRR------EVEIQSHL-R---HPNILRLYGYFH--D------ATRVYLILEYAPLGTVYRELQ---KLS---KFDEQRTATYITELANALSYCHS-KRVIHRDIKPENLLLGSAGEL---KIADFGWSV------HAPSSRRTT---LCGTLDYLPPEMIE---GRMHDEKVDLWSLGVLCYEFLV-GKPP---------------FEAN---TYQETY-KRISRVEFTFPDFVT-----------------------------------------EGARD-LISRLLKHNPSQRPMLREVLEHPWI--------------
----FEIGRPLGKGKFGNVYLAREK----KSHFIVALKVLFKS--Q-IEK---EG--V-E-HQLR-----REIEIQAHL-H---HPNILRLYNYFY--D------RRRIYLILEYAPRGELYKELQ---KSC---TFDEQRTATIMEELADALMYCHG-KKVIHRDIKPENLLLGLKGEL---KIADFGWSV------HAPSLRRKT---MCGTLDYLPPEMIE---GRMHNEKVDLWCIGVLCYELLV-GNPP---------------FESA---SHNETY-RRIVKVDLKFPASVP-----------------------------------------MGAQD-LISKLLRHNPSERLPLAQVSAHPWV--------------
---L-RLVRKLGSGQFGEVWMGYYK-----NNMKVAIKTLK-E--G-TM----SP--E-A-FL-G------EANVMKAL-Q---HERLVRLYAVVT---------KEPIYIVTEYMARGCLLDFLK----TDEGSRLSLPRLIDMSAQIAEGMAYIER-MNSIHRDLRAANILVSEALCC---KIADFGLARII-------DSEYTAQEGAKFPIKWTAPEAIH---FGVFTIKADVWSFGVLLMEVVTYGRVP---------------YPGMSNPEVIRNL-ERGYRMPRPDTCP-------------------------------------------PELYRGVIAECWRSRPEERPTFEFLQSV--L--------------
----YTKIEKIGEGTYGVVYKGRHK----TTGQVVAMKKIRLE--S-EEE---GV--P-S-TAIR------EISLLKEL-R---HPNIVSLQDVLM--Q------DSRLYLIFEFLSM-DLKKYLDSIPPGQ---YMDSSLVKSYLYQILQGIVFCHS-RRVLHRDLKPQNLLIDDKGTI---KLADFGLARAF----GIPIRVYTH---EVVTLWYRSPEVLL--GSARYSTPVDIWSIGTIFAELAT-KKPL---------------FHGDSEIDQLFRI-FRALGTPNNEVWPEVESLQDYKNTFPKWKP---------------GSLASHVKNLDENGLD-LLSKMLIYDPAKRISGKMALNHPYF--------------
---Y-EPVAEIGVGAYGTVYKARDP----HSGHFVALKSVRVP--N-GGGGGGGL--P-I-STVR------EVALLRRL-EAFEHPNVVRLMDVCA--TSR-TDREIKVTLVFEHVDQ-DLRTYLDK-APPP---GLPAETIKDLMRQFLRGLDFLHA-NCIVHRDLKPENILVTSGGTV---KLADFGLARI-----YSYQMALTP---VVVTLWYRAPEVLL---QSTYATPVDMWSVGCIFAEMFR-RKPL---------------FCGNSEADQLGKI-FDLIGLPPEDDWPRDVSLPRGAFPPRGPR-----------------PVQSVVPEMEESGAQ-LLLEMLTFNPHKRISAFRALQHSYL--------------
---W-DLVQTLGEGAYGEVQLAVNR----VTEEAVAVKIVDMK--R-AVD---CP--E-N-I-KK------EICINKML-N---HENVVKFYGHRR--E------GNIQYLFLEYCSGGELFDRIE---PDI---GMPEPDAQRFFHQLMAGVVYLHG-IGITHRDIKPENLLLDERDNL---KISDFGLATVFR--YNNRERLLNK---MCGTLPYVAPELLK--RREFHAEPVDVWSCGIVLTAMLA-GELP---------------WDQP--SDSCQEY-SDWKEKKTYLNPWKKI--D-------------------------------------SAPLA-LLHKILVENPSARITIPDIKKDRW-Y-------------
--YI--MSKTLGSGACGEVKLAFER----KTCKKVAIKIISKR--K-FAI---GS--A-R-EADPALNVETEIEILKKL-N---HPCIIKIKNFF---D------AEDYYIVLELMEGGELFDKVV---GNK---RLKEATCKLYFYQMLLAVQYLHE-NGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKI-----LGETSLMRT---LCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLS-GYPP---------------FSEH--RTQVSLK-DQITSGKYNFIPEVWAEVS-------------------------------------EKALD-LVKKLLVVDPKARFTTEEALRHPWL--------------
----ITLERRLGTGCFGDVWLGTWN-----GSTKVAVKTLK-P--G-TM----SP--K-A-FL-E------EAQVMKLL-R---HDKLVQLYAVVS---------EEPIYIVTEFMCHGSLLDFLK----NPEGQDLRLPQLVDMAAQVAEGMAYMER-MNYIHRDLRAANILVGERLAC---KIADFGLARLI----K--DDEYNPCQGSKFPIKWTAPEAAL---FGRFTIKSDVWSFGILLTELITKGRIP---------------YPGMNKREVLEQV-EQGYHMPCPPGCP-------------------------------------------ASLYE-AMEQTWRLDPEERPTFEYLQSFL----------------
----LQLIKRLGNGQFGEVWMGTWN-----GNTKVAIKTLK-P--G-TM----SP--E-S-FL-E------EAQIMKKL-K---HDKLVQLYAVVS---------EEPIYIVTEYMNKGSLLDFLK----DGEGRALKLPNLVDMAAQVAAGMAYIER-MNYIHRDLRSANILVGNGLIC---KIADFGLARLI----E--DNEYTARQGAKFPIKWTAPEAAL---YGRFTIKSDVWSFGILLTELVTKGRVP---------------YPGMNNREVLEQV-ERGYRMPCPQDCP-------------------------------------------ISLHE-LMIHCWKKDPEERPTFEYLQSFL----------------
----LKLEKKLGAGQFGEVWMATYN-----KHTKVAVKTMK-P--G-SM----SV--E-A-FL-A------EANVMKTL-Q---HDKLVKLHAVVT---------KEPIYIITEFMAKGSLLDFLK----SDEGSKQPLPKLIDFSAQIAEGMAFIEQ-RNYIHRDLRAANILVSASLVC---KIADFGLARVI----E--DNEYTAREGAKFPIKWTAPEAIN---FGSFTIKSDVWSFGILLMEIVTYGRIP---------------YPGMSNPEVIRAL-ERGYRMPRPENCP-------------------------------------------EELYN-IMMRCWKNRPEERPTFEYIQSV--L--------------
-VGG----NKMGEGGFGVVYKGYVN----NTT--VAVKKLAAM--V-DIT---TE--ELK-QQFD-----QEIKVMAKC-Q---HENLVELLGFSS--D------GDDLCLVYVYMPNGSLLDRLSCLDGTP---PLSWHMRCKIAQGAANGINFLHE-NHHIHRDIKSANILLDEAFTA---KISDFGLARASE--KFAQTVMTSR---IVGTTAYMAPEALR----GEITPKSDIYSFGVVLLEIIT-GLPA---------------VDEHREPQLLLDI-KEEIEDEEKTIEDYIDKKMNDAD-----------------------------STSVEAMYS-VASQCLHEKKNKRPDIKKVQQ----LLQ-----------
----LKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQ-H--S-TE-----EHLR-D-FE-R------EIEILKSL-Q---HDNIVKYKGVCYSAG------RRNLKLIMEYLPYGSLRDYLQ----KH-KERIDHIKLLQYTSQICKGMEYLGT-KRYIHRDLATRNILVENENRV---KIGDFGLTKVL-PQ-D--KEYYKVKEPGESPIFWYAPESLT---ESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELL-KNNGRLPRPDGCP-------------------------------------------DEIYM-IMTECWNNNVNQRPSFRDLA----LRV------------
----LKLVERLGAGQFGEVWMGYYN-----GHTKVAVKSLK-Q--G-SM----SP--D-A-FL-A------EANLMKQL-Q---HQRLVRLYAVVT---------QEPIYIITEYMENGSLVDFLK----TPSGIKLTINKLLDMAAQIAEGMAFIEE-RNYIHRDLRAANILVSDTLSC---KIADFGLARLI----E--DNEYTAREGAKFPIKWTAPEAIN---YGTFTIKSDVWSFGILLTEIVTHGRIP---------------YPGMTNPEVIQNL-ERGYRMVRPDNCP-------------------------------------------EELYQ-LMRLCWKERPEDRPTFDYLRSV--L--------------
----FEKISELGAGNGGVVFKVSHK----PSGL-VMARKLIHL--E-IKP---AI--R-N-QIIR------ELQVLHEC-N---SPYIVGFYGAFY--S------DGEISICMEHMDGGSLDQVLK---KAG---RIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEI---KLCDFGVSG------QLIDSMANS---FVGTRSYMSPERLQ---GTHYSVQSDIWSMGLSLVEMAV-GRYP---------------IPPPDAKELELMF-GCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQD-FVNKCLIKNPAERADLKQLMVHAFI--------------
----YQNLSPVGSGAYGSVCAAFDT----KTGLRVAVKKLSRP--F-QSI---IH--A-K-RTYR------ELRLLKHM-K---HENVIGLLDVFT--PARSLEEFNDVYLVTHLMGA-DLNNIVK----CQ---KLTDDHVQFLIYQILRGLKYIHS-ADIIHRDLKPSNLAVNEDCEL---KILDFGLAR-------HTDDEMTG---YVATRWYRAPEIML--NWMHYNQTVDIWSVGCIMAELLT-GRTL---------------FPGTDHIDQLKLI-LRLVGTPGAELLKKISSESARNYIQSLTQMP-------------KMNFANVFIGANPLAVD-LLEKMLVLDSDKRITAAQALAHAYF--------------
---F-KFGKILGEGSFSTVVLAREL----ATSREYAIKILEKR--H-IIK---EN--K-VPYVTR------ERDVMSRL-D---HPFFVKLYFTFQ--D------DEKLYFGLSYAKNGELLKYIR---KIG---SFDETCTRFYTAEIVSALEYLHG-KGIIHRDLKPENILLNEDMHI---QITDFGTAKVLS--PESKQARANS---FVGTAQYVSPELLT---EKSACKSSDLWALGCIIYQLVA-GLPP---------------FRAG---NEYLIF-QKIIKLEYDFPEKFF-----------------------------------------PKARD-LVEKLLVLDATKRLGCEEM------EGYGPLKAHPF---
---F-NFLMVLGKGSFGKVMLADRK----GTEELYAIKILKKD--V-VIQ---DD--D-V-ECTM-----VEKRVLALLDK---PPFLTQLHSCFQ--T------VDRLYFVMEYVNGGDLMYHIQ---QVG---KFKEPQAVFYAAEISIGLFFLHK-RGIIYRDLKLDNVMLDSEGHI---KIADFGMCKEH----MMDGVTTRT---FCGTPDYIAPEIIA---YQPYGKSVDWWAYGVLLYEMLA-GQPP---------------FDGE---DEDELF-QSIMEHNVSYPKSLS-----------------------------------------KEAVS-VCKGLMTKHPAKRLGC---------GPEGERDVREHAFF
----IELGRCIGEGQFGDVHQGIYM-SPENPALAVAIKTCK-N--C-TS----DSVRE-K-FL-Q------EALTMRQF-D---HPHIVKLIGVIT---------ENPVWIIMELCTLGELRSFLQ----VR-KYSLDLASLILYAYQLSTALAYLES-KRFVHRDIAARNVLVSSNDCV---KLGDFGLSRYM----E--DSTYYKASKGKLPIKWMAPESIN---FRRFTSASDVWMFGVCMWEILMHGVKP---------------FQGVKNNDVIGRI-ENGERLPMPPNCP-------------------------------------------PTLYS-LMTKCWAYDPSRRPRFTELKAQL----------------
---F-TLCRKLGSGYFGEVFEGLWK-----DRVQVAIKVIS-R--D-NL----LH-QQ-M-LQ-S------EIQAMKKL-R---HKHILALYAVVS--V------GDPVYIITELMAKGSLLELLR----DSDEKVLPVSELLDIAWQVAEGMCYLES-QNYIHRDLAARNILVGENTLC---KVGDFGLARLI-------KEDVYLSHDHNIPYKWTAPEALS---RGHYSTKSDVWSFGILLHEMFSRGQVP---------------YPGMSNHEAFLRV-DAGYRMPCPLECP-------------------------------------------PSVHK-LMLTCWCRDPEQRPCFKALRERL----------------
---L-RLEVKLGQGCFGEVWMGTWN-----GTTRVAIKTLK-P--G-TM----SP--E-A-FL-Q------EAQVMKKL-R---HEKLVQLYAVVS---------EEPIYIVTEYMSKGSLLDFLK----GETGKYLRLPQLVDMAAQIASGMAYVER-MNYVHRDLRAANILVGENLVC---KVADFGLARLI----E--DNEYTARQGAKFPIKWTAPEAAL---YGRFTIKSDVWSFGILLTELTTKGRVP---------------YPGMVNREVLDQV-ERGYRMPCPPECP-------------------------------------------ESLHD-LMCQCWRKEPEERPTFEYLQAF--L--------------
--FA--LGRKLGEGYFGEVWEGLWL-----GSLPVAIKVIK-S--A-NM----K--LT-D-LA-K------EIQTLKGL-R---HERLIRLHAVCS--G------GEPVYIVTELMRKGNLQAFLG----TPEGRALRLPPLLGFACQVAEGMSYLEE-QRVVHRDLAARNVLVDDGLAC---KVADFGLARLL----K--DDIYSPSSSSKIPVKWTAPEAAN---YRVFSQKSDVWSFGVLLHEVFTYGQCP---------------YEGMTNHETLQQI-MRGYRLPRPAACP-------------------------------------------AEVYV-LMLECWRSSPEERPSFATLREKL----------------
---L-RLLEKLGDGSFGVVRRGEWD-APSGKTVSVAVKCLK-PDVL-SQ----PEAMD-D-FI-R------EVNAMHSL-D---HRNLIRLYGVVL---------TPPMKMVTELAPLGSLLDRLR----KH-QGHFLLGTLSRYAVQVAEGMGYLES-KRFIHRDLAARNLLLATRDLV---KIGDFGLMRAL-PQ-N--DDHYVMQEHRKVPFAWCAPESLK---TRTFSHASDTWMFGVTLWEMFTYGQEP---------------WIGLNGSQILHKIDKEGERLPRPEDCP-------------------------------------------QDIYN-VMVQCWAHKPEDRPTFVALRDF--L--------------
---L-RLEVKLGQGCFGEVWMGTWN-----GTTKVAIKTLK-P--G-TM----MP--E-A-FL-Q------EAQIMKKL-R---HDKLVPLYAVVS---------EEPIYIVTEFMSKGSLLDFLK----EGDGKYLKLPQLVDMAAQIADGMAYIER-MNYIHRDLRAANILVGENLVC---KIADFGLARLI----E--DNEYTARQGAKFPIKWTAPEAAL---YGRFTIKSDVWSFGILQTELVTKGRVP---------------YPGMVNREVLEQV-ERGYRMPCPQGCP-------------------------------------------ESLHE-LMNLCWKKDPDERPTFEYIQSFL----------------
LIAD----IELGCGNFGSVRQGVYR--MRKKQIDVAIKVLK-Q--G-TE----KADTE-E-MM-R------EAQIMHQL-D---NPYIVRLIGVCQ---------AEALMLVMEMAGGGPLHKFLV----GK-REEIPVSNVAELLHQVSMGMKYLEE-KNFVHRDLAARNVLLVNRHYA---KISDFGLSKAL-GA-D--DSYYTARSAGKWPLKWYAPECIN---FRKFSSRSDVWSYGVTMWEALSYGQKP---------------YKKMKGPEVMAFI-EQGKRMECPPECP-------------------------------------------PELYA-LMSDCWIYKWEDRPDFLTVEQR--M--------------
|