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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
CAMK CHEK1 203
AGC AKT1 150
Other AURKA 133
Other AURKB 77
Other AURKC 43
TKL BRAF 456
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK4 6
CMGC CDK5 4
CMGC CSNK2A1 39
CMGC GSK3A 119
CMGC GSK3B 56
TK HCK 241
TK LYN 247
CMGC MAPK14 24
CAMK MAPKAPK2 64
Other NEK2 8
TK NTRK1 510
AGC PDPK1 82
CAMK PIM1 38
AGC PRKCA 339
AGC PRKCB 342
AGC PRKCD 349
AGC PRKCQ 380
AGC PRKCZ 252
CAMK SIK1 27
TK SRC 270
CAMK STK11 49
--W-DLVQTLGEGAYGEVQLAVNR-----VTEEAVAVKIVDMK-R-AVD---CP--EN-I-KK----EICINKML-N---HEN--VVKFYGHRRE------GNIQY--LFLEYCSGG--ELFDRIE----PDI----------------GMPEPDAQRFFHQLMAGVVYLHG-I-----GITHRDIKPENLLLD-ERDNL---KISDFGLATVFR-YNNRERLLNK---MCGTLPYVAPELLK-RREF---HAEPVDVWSCGIVLTAMLA-GELPWD-QP------SDSCQEYSDWKE----KKTYLNPWKKI--D------------------------------SAPLALLHKILVENPSARIT------IP-DIKKDRW-Y-------------
--F-EYLKLLGKGTFGKVILVKEK-----ATGRYYAMKILKKE-V-IVA---KD--EV-AHTL---TENRVLQNS-R---HPF--LTALKYSFQT------HDRLC--FVMEYANGG--ELFFHLS----RER----------------VFSEDRARFYGAEIVSALDYLHSEK-----NVVYRDLKLENLMLD-KDGHI---KITDFGLCKEG---IKDGATMKT---FCGTPEYLAPEVLE--DND---YGRAVDWWGLGVVMYEMMC-GRLPFY-NQ-------DHEKLFELILM----EEIRFPRTLG----------------------------------PEAKSLLSGLLKKDPKQRLGGG-SEDAK-EIMQHRFF--------------
---FEIGRPLGKGKFGNVYLAREK-----QSKFILALKVLFKA-QLEKA---GV--EH-QLRR----EVEIQSHL-R---HPN--ILRLYGYFHD------ATRVY--LILEYAPLG--TVYRELQ----KLS----------------KFDEQRTATYITELANALSYCHS-K-----RVIHRDIKPENLLLG-SAGEL---KIADFGWSV-----HAPSSRRTT---LCGTLDYLPPEMIE--GRM---HDEKVDLWSLGVLCYEFLV-GKPPFE-AN-------TYQETYKRISR----VEFTFPDFVT----------------------------------EGARDLISRLLKHNPSQRPM------LR-EVLEHPWI--------------
---FEIGRPLGKGKFGNVYLAREK-----KSHFIVALKVLFKS-Q-IEK---EG--VE-HQLR---REIEIQAHL-H---HPN--ILRLYNYFYD------RRRIY--LILEYAPRG--ELYKELQ----KSC----------------TFDEQRTATIMEELADALMYCHG-K-----KVIHRDIKPENLLLG-LKGEL---KIADFGWSV-----HAPSLRRKT---MCGTLDYLPPEMIE--GRM---HNEKVDLWCIGVLCYELLV-GNPPFE-SA-------SHNETYRRIVK----VDLKFPASVP----------------------------------MGAQDLISKLLRHNPSERLP------LA-QVSAHPWV--------------
---FEIGRPLGKGKFGNVYLARLK-----ESHFIVALKVLFKS-Q-IEK---EG--LE-HQLR---REIEIQAHL-Q---HPN--ILRLYNYFHD------ARRVY--LILEYAPRG--ELYKELQ----KSE----------------KLDEQRTATIIEELADALTYCHD-K-----KVIHRDIKPENLLLG-FRGEV---KIADFGWSV-----HTPSLRRKT---MCGTLDYLPPEMIE--GRT---YDEKVDLWCIGVLCYELLV-GYPPFE-SA-------SHSETYRRILK----VDVRFPLSMP----------------------------------LGARDLISRLLRYQPLERLP------LA-QILKHPWV--------------
---ITVGQRIGSGSFGTVYKGKWH--------GDVAVKMLN-VTA-PT----PQQLQA-FK-N----EVGVLRKT-R---HVN--ILLFMGYST-------KPQLA--IVTQWCEGS--SLYHHLH-----II--------------ETKFEMIKLIDIARQTAQGMDYLHA-K-----SIIHRDLKSNNIFLH-EDLTV---KIGDFGLATVK-S-R--WSGSHQFEQLSGSILWMAPEVIR--MQDKNPYSFQSDVYAFGIVLYELMT-GQLPYS-NI-N--NRDQIIFMVGRGYLSPDLSKVRSNCP------------------------------------KAMKRLMAECLKKKRDERPL------FP-QILA---SI-------------
---YTKIEKIGEGTYGVVYKGRHK-----TTGQVVAMKKIRLE-S-EEE---GV--PS-TAIR----EISLLKEL-R---HPN--IVSLQDVLMQ------DSRLY--LIFEFLSM---DLKKYLDS-IPPGQ----------------YMDSSLVKSYLYQILQGIVFCHS-R-----RVLHRDLKPQNLLID-DKGTI---KLADFGLARAF---GIPIRVYTH---EVVTLWYRSPEVLL-GSAR---YSTPVDIWSIGTIFAELAT-KKPLFH-GD----SEIDQLFRIFRALG----TPNNEVWPEVESLQDYKNTFPKWKP--------GSLASHVKNLDENGLDLLSKMLIYDPAKRIS------GK-MALNHPYF--------------
---FQKVEKIGEGTYGVVYKARNK-----LTGEVVALKKIRLD-T-ETE---GV--PS-TAIR----EISLLKEL-N---HPN--IVKLLDVIHT------ENKLY--LVFEFLHQ---DLKKFMDA--SALT----------------GIPLPLIKSYLFQLLQGLAFCHS-H-----RVLHRDLKPQNLLIN-TEGAI---KLADFGLARAF---GVPVRTYTH---EVVTLWYRAPEILL-GCKY---YSTAVDIWSLGCIFAEMVT-RRALFP-GD----SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR--------QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS------AK-AALAHPFF--------------
--Y-EPVAEIGVGAYGTVYKARDP-----HSGHFVALKSVRVP-N-GGGGGGGL--PI-STVR----EVALLRRL-EAFEHPN--VVRLMDVCATSR-TDREIKVT--LVFEHVDQ---DLRTYLDK--APPP----------------GLPAETIKDLMRQFLRGLDFLHA-N-----CIVHRDLKPENILVT-SGGTV---KLADFGLARI----YSYQMALTP---VVVTLWYRAPEVLL--QST---YATPVDMWSVGCIFAEMFR-RKPLFC-GN----SEADQLGKIFDLIG----LPPEDDWPRDVSLPRGAFPPRGPR----------PVQSVVPEMEESGAQLLLEMLTFNPHKRIS------AF-RALQHSYL--------------
---YEKLEKIGEGTYGTVFKAKNR-----ETHEIVALKRVRLD-D-DDE---GV--PS-SALR----EICLLKEL-K---HKN--IVRLHDVLHS------DKKLT--LVFEFCDQ---DLKKYFD---SCNG----------------DLDPEIVKSFLFQLLKGLGFCHS-R-----NVLHRDLKPQNLLIN-RNGEL---KLADFGLARAF---GIPVRCYSA---EVVTLWYRPPDVLF-GAKL---YSTSIDMWSAGCIFAELANAGRPLFP-GN----DVDDQLKRIFRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT--------TSLVNVVPKLNATGRDLLQNLLKCNPVQRIS------AE-EALQHPYF--------------
---YQLVRKLGRGKYSEVFEAINI-----TNNEKVVVKILKPV-K-KKK---IK--R----------EIKILENLRG---GPN--IITLADIVKDP----VSRTPA--LVFEHVNNT--DFKQLY-------Q----------------TLTDYDIRFYMYEILKALDYCHS-M-----GIMHRDVKPHNVMIDHEHRKL---RLIDWGLAEF----YHPGQEYNV---RVASRYFKGPELLV-DYQM---YDYSLDMWSLGCMLASMIF-RKEPFFHGH----DNYDQLVRIAKVLG----TEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLT------AR-EAMEHPYF--------------
---YTDIKVIGNGSFGVVYQARLA-----ETRELVAIKKVLQD-K-RFK---NR-------------ELQIMRKL-D---HCN--IVRLRYFFYSSG--EKKDELYLNLVLEYVPETVYRVARHFT---KAKL----------------TIPILYVKVYMYQLFRSLAYIHS-Q-----GVCHRDIKPQNLLVDPDTAVL---KLCDFGSAKQ----LVRGEPNVS---YICSRYYRAPELIF-GATD---YTSSIDVWSAGCVLAELLL-GQPIFP-GD----SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH--------PWTKVFKSRTPPEAIALCSSLLEYTPSSRLS------PL-EACAHSFF--------------
---YTDTKVIGNGSFGVVYQAKLC-----DSGELVAIKKVLQD-K-RFK---NR-------------ELQIMRKL-D---HCN--IVRLRYFFYSSG--EKKDEVYLNLVLDYVPETVYRVA---RHYSRAKQ----------------TLPVIYVKLYMYQLFRSLAYIHS-F-----GICHRDIKPQNLLLDPDTAVL---KLCDFGSAKQ----LVRGEPNVS---YICSRYYRAPELIF-GATD---YTSSIDVWSAGCVLAELLL-GQPIFP-GD----SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH--------PWTKVFRPRTPPEAIALCSRLLEYTPTARLT------PL-EACAHSFF--------------
---LKLEKKLGAGQFGEVWMATYN------KHTKVAVKTMK-P-G-SM----SV--EA-FL-A----EANVMKTL-Q---HDK--LVKLHAVVT-------KEPIY--IITEFMAKG--SLLDFLK-----SD-------------EGSKQPLPKLIDFSAQIAEGMAFIEQ-R-----NYIHRDLRAANILVS-ASLVC---KIADFGLARVI---E--DNEYTAREGAKFPIKWTAPEAIN--FGS---FTIKSDVWSFGILLMEIVTYGRIPYP-GM----SNPEVIRALERGYR----MPRPENCP------------------------------------EELYNIMMRCWKNRPEERPT------FE-YIQSV--L--------------
---IKLVKRLGAGQFGEVWMGYYN------NSTKVAVKTLK-P-G-TM----SV--QA-FL-E----EANLMKTL-Q---HDK--LVRLYAVVTR------EEPIY--IITEYMAKG--SLLDFLK-----SD-------------EGGKVLLPKLIDFSAQIAEGMAYIER-K-----NYIHRDLRAANVLVS-ESLMC---KIADFGLARVI---E--DNEYTAREGAKFPIKWTAPEAIN--FGC---FTIKSDVWSFGILLYEIVTYGKIPYP-GR----TNADVMTALSQGYR----MPRVENCP------------------------------------DELYDIMKMCWKEKAEERPT------FD-YLQSV--L--------------
---YQNLSPVGSGAYGSVCAAFDT-----KTGLRVAVKKLSRP-F-QSI---IH--AK-RTYR----ELRLLKHM-K---HEN--VIGLLDVFTPARSLEEFNDVY--LVTHLMGA---DLNNIVK-----CQ----------------KLTDDHVQFLIYQILRGLKYIHS-A-----DIIHRDLKPSNLAVN-EDCEL---KILDFGLAR------HTDDEMTG---YVATRWYRAPEIML-NWMH---YNQTVDIWSVGCIMAELLT-GRTLFP-GT----DHIDQLKLILRLVG----TPGAELLKKISSESARNYIQSLTQMP------KMNFANVFIGANPLAVDLLEKMLVLDSDKRIT------AA-QALAHAYF--------------
KVT---SQVLGLGINGKVLQIFNK-----RTQEKFALKMLQDC-P-KAR-------------R----EVELHWRASQ---CPH--IVRIVDVYENLY--AGRKCLL--IVMECLDGG--ELFSRIQD--RGDQ----------------AFTEREASEIMKSIGEAIQYLHS-I-----NIAHRDVKPENLLYT-SKRPNAILKLTDFGFAKE----TTSHNSLTT---PCYTPYYVAPEVLG--PEK---YDKSCDMWSLGVIMYILLC-GYPPFY-SN---HGLAISPGMKTRIRM----GQYEFPNPEWSEVS------------------------------EEVKMLIRNLLKTEPTQRMT------IT-EFMNHPWI--------------
---YEVLYTIGTGSYGRCQKIRRK-----SDGKILVWKELDYG-S-MTE---AE--KQ-MLVS----EVNLLREL-K---HPN--IVRYYDRIIDR----TNTTLY--IVMEYCEGG--DLASVITKGTKERQ----------------YLDEEFVLRVMTQLTLALKECHR-RSDGGHTVLHRDLKPANVFLD-GKQNV---KLGDFGLARIL---NHDTSFAKT---FVGTPYYMSPEQMN--RMS---YNEKSDIWSLGCLLYELCA-LMPPFT-AF-------SQKELAGKIRE----GKFRRIPYRY---S------------------------------DELNEIITRMLNLKDYHRPS------VE-EILENPL-I-------------
-IV--LKWELGEGAFGKVFLAECHNLLPEQDKMLVAVKALK-E-A-SE----SA-RQD-FQ-R----EAELLTML-Q---HQH--IVRFFGVCTE------GRPLL--MVFEYMRHG--DLNRFLR-----SHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLAG-L-----HFVHRDLATRNCLVG-QGLVV---KIGDFGMSRDI-Y-S--TDYYRVGGRTMLPIRWMPPESIL--YRK---FTTESDVWSFGVVLWEIFTYGKQPWY-QL----SNTEAIDCITQGRE----LERPRACP------------------------------------PEVYAIMRGCWQREPQQRHS------IK-DVHARL----------------
--F-KFGKILGEGSFSTVVLAREL-----ATSREYAIKILEKR-H-IIK---EN--KVPYVTR----ERDVMSRL-D---HPF--FVKLYFTFQD------DEKLY--FGLSYAKNG--ELLKYIR----KIG----------------SFDETCTRFYTAEIVSALEYLHG-K-----GIIHRDLKPENILLN-EDMHI---QITDFGTAKVLS-PESKQARANS---FVGTAQYVSPELLT--EKS---ACKSSDLWALGCIIYQLVA-GLPPFR-AG-------NEYLIFQKIIK----LEYDFPEKFF----------------------------------PKARDLVEKLLVLDATKRLG------CE-EM------EGYGPLKAHPF---
---YQVGPLLGSGGFGSVYSGIRV-----SDNLPVAIKHVEKD-R-ISD---WG--EL-PNGTRVPMEVVLLKKV-S---SGFSGVIRLLDWFER------PDSFV--LILERPEPV-QDLFDFIT----ERG----------------ALQEELARSFFWQVLEAVRHCHN-C-----GVLHRDIKDENILIDLNRGEL---KLIDFGSGA-----LLKDTVYTD---FDGTRVYSPPEWIR-YHRY---HGRSAAVWSLGILLYDMVC-GDIPFE-HD-------------EEIIR----GQVFFRQRVS----------------------------------SECQHLIRWCLALRPSDRPT------FE-EIQNHPWM--------------
--F-NFLMVLGKGSFGKVMLADRK-----GTEELYAIKILKKD-V-VIQ---DD--DV-ECTM---VEKRVLALLDK---PPF--LTQLHSCFQT------VDRLY--FVMEYVNGG--DLMYHIQ----QVG----------------KFKEPQAVFYAAEISIGLFFLHK-R-----GIIYRDLKLDNVMLD-SEGHI---KIADFGMCKEH---MMDGVTTRT---FCGTPDYIAPEIIA--YQP---YGKSVDWWAYGVLLYEMLA-GQPPFD-GE-------DEDELFQSIME----HNVSYPKSLS----------------------------------KEAVSVCKGLMTKHPAKRLG------C----------GPEGERDVREHAFF
--F-NFLMVLGKGSFGKVMLSERK-----GTDELYAVKILKKD-V-VIQ---DD--DV-ECTM---VEKRVLALPGK---PPF--LTQLHSCFQT------MDRLY--FVMEYVNGG--DLMYHIQ----QVG----------------RFKEPHAVFYAAEIAIGLFFLQS-K-----GIIYRDLKLDNVMLD-SEGHI---KIADFGMCKEN---IWDGVTTKT---FCGTPDYIAPEIIA--YQP---YGKSVDWWAFGVLLYEMLA-GQAPFE-GE-------DEDELFQSIME----HNVAYPKSMS----------------------------------KEAVAICKGLMTKHPGKRLG------C----------GPEGERDIKEHAFF
-FI--FHKVLGKGSFGKVLLGELK-----GRGEYFAIKALKKD-V-VLI---DD--DV-ECTM---VEKRVLTLAAE---NPF--LTHLICTFQT------KDHLF--FVMEFLNGG--DLMYHIQ----DKG----------------RFELYRATFYAAEIMCGLQFLHS-K-----GIIYRDLKLDNVLLD-RDGHI---KIADFGMCKEN---IFGESRAST---FCGTPDYIAPEILQ--GLK---YTFSVDWWSFGVLLYEMLI-GQSPFH-GD-------DEDELFESIRV----DTPHYPRWIT----------------------------------KESKDILEKLFEREPTKRLG-----VTG-NIKIHPFF--------------
-FI--LHKMLGKGSFGKVFLAEFK-----KTNQFFAIKALKKD-V-VLM---DD--DV-ECTM---VEKRVLSLAWE---HPF--LTHMFCTFQT------KENLF--FVMEYLNGG--DLMYHIQ----SCH----------------KFDLSRATFYAAEIILGLQFLHS-K-----GIVYRDLKLDNILLD-KDGHI---KIADFGMCKEN---MLGDAKTNT---FCGTPDYIAPEILL--GQK---YNHSVDWWSFGVLLYEMLI-GQSPFH-GQ-------DEEELFHSIRM----DNPFYPRWLE----------------------------------KEAKDLLVKLFVREPEKRLG------VRGDIRQHPLF--------------
--F-DLIRVIGRGSYAKVLLVRLK-----KNDQIYAMKVVKKE-L-VHD---DE--DI-DWVQ---TEKHVFEQASS---NPF--LVGLHSCFQT------TSRLF--LVIEYVNGG--DLMFHMQ----RQR----------------KLPEEHARFYAAEICIALNFLHE-R-----GIIYRDLKLDNVLLD-ADGHI---KLTDYGMCKEG---LGPGDTTST---FCGTPNYIAPEILR--GEE---YGFSVDWWALGVLMFEMMA-GRSPFD-IITDNPDMNTEDYLFQVILE----KPIRIPRFLS----------------------------------VKASHVLKGFLNKDPKERLGCRPQTGF----------SDIKSHAFF-----
---YDIERTLGKGNFAVVKLARHR-----VTKTQVAIKIIDKT-R-LDS---SN--LE-KIYR----EVQLMKLL-N---HPH--IIKLYQVMET------KDMLY--IVTEFAKNG--EMFDYLT----SNG----------------HLSENEARKKFWQILSAVEYCHD-H-----HIVHRDLKTENLLLD-GNMDI---KLADFGFGNF----YKSGEPLST---WCGSPPYAAPEVFE-GKEY---EGPQLDIWSLGVVLYVLVC-GSLPFD-GP-------NLPTLRQRVLE----GRFRIPFFMS----------------------------------QDCESLIRRMLVVDPARRIT------IA-QIRQHRWM--------------
--L-RLEVKLGQGCFGEVWMGTWN------GTTRVAIKTLK-P-G-TM----SP--EA-FL-Q----EAQVMKKL-R---HEK--LVQLYAVVS-------EEPIY--IVTEYMSKG--SLLDFLK-----GE-------------TGKYLRLPQLVDMAAQIASGMAYVER-M-----NYVHRDLRAANILVG-ENLVC---KVADFGLARLI---E--DNEYTARQGAKFPIKWTAPEAAL--YGR---FTIKSDVWSFGILLTELTTKGRVPYP-GM----VNREVLDQVERGYR----MPCPPECP------------------------------------ESLHDLMCQCWRKEPEERPT------FE-YLQAF--L--------------
YLM---GDLLGEGSYGKVKEVLDS-----ETLCRRAVKILKKK-K-LRR---IP--NG-EANVK--KEIQLLRRL-R---HKN--VIQLVDVLYNE----EKQKMY--MVMEYCVCGMQEMLDSVP-----EK----------------RFPVCQAHGYFCQLIDGLEYLHS-Q-----GIVHKDIKPGNLLLT-TGGTL---KISDLGVAEALH-PFAADDTCRT---SQGSPAFQPPEIANGLDTF---SGFKVDIWSAGVTLYNITT-GLYPFE-GD-------NIYKLFENIGK----GSYAIPGDCG----------------------------------PPLSDLLKGMLEYEPAKRFS------IR-QIRQHSWF--------------
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