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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK EPHA3 203
TK ABL1 261
Other AURKA 133
TK EGFR 712
TK EPHA2 613
TK ERBB3 709
TK FGFR2 481
TK IGF1R 999
TK INSR 1023
TK KIT 589
TK LCK 245
Other NEK2 8
Other NEK7 34
AGC PDPK1 82
TK PTK2B 425
TK RET 724
TK SRC 270
TK TNK2 150
I-SIDKVVGAGEFGEVCSGRLK----LPSKKEISVAIKTLK-V--G-YT-EKQRRD-FL-G-EASIMGQF-DHPNIIRLEGVVTK--SKPVMIVTEYMENGSLDSFLR-----KH---------------------------------------------------------------------------DAQFTVIQLVGMLRGIASGMKYLSDM-----GYVHRDLAARNILINSNLVCKVSDFGLSRVL--E-D--DPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEM-SNQDVIKAV-DEGYRLPPPMDCP-----AALYQLMLDCWQKDRNNRPKFEQIVSIL-------------
-ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-E--D-TM-EV--EE-FL-K-EAAVMKEI-KHPNLVQLLGVCTR--EPPFYIITEFMTYGNLLDYLR-----EC--------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKK-----NFIHRDLAARNCLVGENHLVKVADFGLSRLM----T--GDTY-TAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGI-DLSQVYELL-EKDYRMERPEGCP-----EKVYELMRACWQWNPSDRPSFAEIHQA--F-----------
-FEIGRPLGKGKFGNVYLAREK-------QSKFILALKVLFKA--QLEKAGV--EH-QLRR-EVEIQSHL-RHPNILRLYGYFHD--ATRVYLILEYAPLGTVYRELQ----KLS-----------------------------------------------------------------------------KFDEQRTATYITELANALSYCHSK-----RVIHRDIKPENLLLGSAGELKIADFGWSV------HAPSSRR-TT---LCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLV-GKPPFEAN----TYQETY-KRISRVEFTFPDFVT---EGARDLISRLLKHNPSQRPMLREVLEHPWI-----------
F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E--A-TS-PKANKE-IL-D-EAYVMASV-DNPHVCRLLGICL---TSTVQLITQLMPFGCLLDYVR-----EH---------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDR-----RLVHRDLAARNVLVKTPQHVKITDFGLAKLL--G-A--EEKE-YHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGI-PASEISSIL-EKGERLPQPPICT-----IDVYMIMVKCWMIDADSRPKFRELI----IEF---------
V-TRQKVIGAGEFGEVYKGMLKT---SSGKKEVPVAIKTLK-A--G-YT-EKQRVD-FL-G-EAGIMGQF-SHHNIIRLEGVISK--YKPMMIITEYMENGALDKFLR-----EK---------------------------------------------------------------------------DGEFSVLQLVGMLRGIAAGMKYLANM-----NYVHRDLAARNILVNSNLVCKVSDFGLSRVL--E-D--DPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWEL-SNHEVMKAI-NDGFRLPTPMDCP-----SAIYQLMMQCWQQERARRPKFADIVSIL-------------
L-RKLKVLGSGVFGTVHKGVWIP---EGESIKIPVCIKVIE-D--K-SG-RQSFQA-VT-D-HMLAIGSL-DHAHIVRLLGLCP---GSSLQLVTQYLPLGSLLDHVR-----QH---------------------------------------------------------------------------RGALGPQLLLNWGVQIAKGMYYLEEH-----GMVHRNLAARNVLLKSPSQVQVADFGVADLL--P-P--DDKQ-LLYSEAKTPIKWMALESIHFGKYTHQSDVWSYGVTVWELMTFGAEPYAGL-RLAEVPDLL-EKGERLAQPQICT-----IDVYMVMVKCWMIDENIRPTFKELANE--F-----------
-LTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLK-D--D-AT-EKDLSD-LV-S-EMEMMKMIGKHKNIINLLGACTQ--DGPLYVIVEYASKGNLREYLR-----ARRPPGMEY------------------------------------------------------------SYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQ-----KCIHRDLAARNVLVTENNVMKIADFGLARDI--N-N--IDYY-KKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGI-PVEELFKLL-KEGHRMDKPANCT-----NELYMMMRDCWHAVPSQRPTFKQLVEDL-------------
-ITMSRELGQGSFGMVYEGVAKG--VVKDEPETRVAIKTVN-E--A-AS-MRERIE-FL-N-EASVMKEF-NCHHVVRLLGVVSQ--GQPTLVIMELMTRGDLKSYLR-----SLRPEM------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNAN-----KFVHRDLAARNCMVAEDFTVKIGDFGMTRDI--Y-E--TDYY-RKGGKGLLPVRWMSPESLKDGVFTTYSDVWSFGVVLWEIATLAEQPYQGL-SNEQVLRFV-MEGGLLDKPDNCP-----DMLFELMRMCWQYNPKMRPSFLEIISS--I-----------
-ITLLRELGQGSFGMVYEGNARD--IIKGEAETRVAVKTVN-E--S-AS-LRERIE-FL-N-EASVMKGF-TCHHVVRLLGVVSK--GQPTLVVMELMAHGDLKSYLR-----SLRPEA------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNAK-----KFVHRDLAARNCMVAHDFTVKIGDFGMTRDI--Y-E--TDYY-RKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGL-SNEQVLKFV-MDGGYLDQPDNCP-----ERVTDLMRMCWQFNPKMRPTFLEIVNLL-------------
-LSFGKTLGAGAFGKVVEATAYG--LIKSDAAMTVAVKMLK-P--S-AH-LTEREA-LM-S-ELKVLSYLGNHMNIVNLLGACTI--GGPTLVITEYCCYGDLLNFLR-----RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASK-----NCIHRDLAARNILLTHGRITKICDFGLARDI--K-N--DSNY-VVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSPYPGMPVDSKFYKMI-KEGFRMLSPEHAP-----AEMYDIMKTCWDADPLKRPTFKQIVQL--I-----------
-LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q--G-SM-SP--DA-FL-A-EANLMKQL-QHQRLVRLYAVVT---QEPIYIITEYMENGSLVDFLK-----TP--------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEER-----NYIHRDLRAANILVSDTLSCKIADFGLARLI----E--DNEY-TAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGM-TNPEVIQNL-ERGYRMVRPDNCP-----EELYQLMRLCWKERPEDRPTFDYLRSV--L-----------
-YEVLYTIGTGSYGRCQKIRRK-------SDGKILVWKELDYG--S-MTEAE--KQ-MLVS-EVNLLREL-KHPNIVRYYDRIIDRTNTTLYIVMEYCEGGDLASVITKGTKERQ-----------------------------------------------------------------------------YLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLDGKQNVKLGDFGLARIL----NHDTSFA-KT---FVGTPYYMSPEQMNRMSYNEKSDIWSLGCLLYELCA-LMPPFTAF----SQKELA-GKIREGKFRRIPYRY--SDELNEIITRMLNLKDYHRPSVEEILENPL-I----------
-FRIEKKIGRGQFSEVYRAACL-------LDGVPVALKKVQIF--D-LMDAK--AR-ADCIKEIDLLKQL-NHPNVIKYYASFIE--DNELNIVLELADAGDLSRMIKHFKKQKR-----------------------------------------------------------------------------LIPERTVWKYFVQLCSALEHMHSR-----RVMHRDIKPANVFITATGVVKLGDLGLGRFF----SSKTTAA-HS---LVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAA-LQSPFYGD--KMNLYSLC-KKIEQCDYPPLPSDHYS-EELRQLVNMCINPDPEKRPDVTYV------YDVA-------
F-KFGKILGEGSFSTVVLAREL-------ATSREYAIKILEKR--H-IIKEN--KVPYVTR-ERDVMSRL-DHPFFVKLYFTFQD--DEKLYFGLSYAKNGELLKYIR----KIG-----------------------------------------------------------------------------SFDETCTRFYTAEIVSALEYLHGK-----GIIHRDLKPENILLNEDMHIQITDFGTAKVLS--PESKQARA-NS---FVGTAQYVSPELLTEKSACKSSDLWALGCIIYQLVA-GLPPFRAG----NEYLIF-QKIIKLEYDFPEKFF---PKARDLVEKLLVLDATKRLGCEEM------EGYGPLKAHPF
V-VLNRILGEGFFGEVYEGVYT----NHKGEKINVAVKTCK-K--D-CT-LDNKEK-FM-S-EAVIMKNL-DHPHIVKLIGIIE---EEPTWIIMELYPYGELGHYLE-----RN---------------------------------------------------------------------------KNSLKVLTLVLYSLQICKAMAYLESI-----NCVHRDIAVRNILVASPECVKLGDFGLSRYI----E--DEDY-YKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWL-ENKDVIGVL-EKGDRLPKPDLCP-----PVLYTLMTRCWDYDPSDRPRFTELVCS--L-----------
L-VLGKTLGEGEFGKVVKATAFH--LKGRAGYTTVAVKMLK-E--N-AS-PSELRD-LL-S-EFNVLKQV-NHPHVIKLYGACSQ--DGPLLLIVEYAKYGSLRGFLR-----ESRKVGPGYLGSG----------------------------------------------------GSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEM-----KLVHRDLAARNILVAEGRKMKISDFGLSRDV--Y-E--EDSY-VKRSQGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGI-PPERLFNLL-KTGHRMERPDNCS-----EEMYRLMLQCWKQEPDKRPVFADISKDL-------------
L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P--G-TM-SP--EA-FL-Q-EAQVMKKL-RHEKLVQLYAVVS---EEPIYIVTEYMSKGSLLDFLK-----GE--------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERM-----NYVHRDLRAANILVGENLVCKVADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGM-VNREVLDQV-ERGYRMPCPPECP-----ESLHDLMCQCWRKEPEERPTFEYLQAF--L-----------
L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVL-SQ-PEAMDD-FI-R-EVNAMHSL-DHRNLIRLYGVVL---TPPMKMVTELAPLGSLLDRLR-----KH---------------------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLESK-----RFIHRDLAARNLLLATRDLVKIGDFGLMRAL-PQ-N--DDHY-VMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGL-NGSQILHKIDKEGERLPRPEDCP-----QDIYNVMVQCWAHKPEDRPTFVALRDF--L-----------
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