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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK JAK2 203
TK ABL1 261
Other AURKA 133
Other AURKB 77
TKL BRAF 456
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK4 6
CMGC CDK5 4
TK EGFR 712
CMGC GSK3A 119
CMGC GSK3B 56
TK HCK 241
TK IGF1R 999
TK INSR 1023
TK ITK 363
TK JAK3 822
TK KIT 589
TK LCK 245
STE MAP2K1 68
TK NTRK1 510
AGC PDPK1 82
TKL RAF1 349
TK SRC 270
--LKFLQQLGKGNFGSVEMCRYDP-LQDNTGEVVAVKKLQ-H-S-TE-----EHLRD-FE-R-EIEILKSL-Q---HDNIVKYKGVCYSAG-----RRNLK--LIMEYLPYG--SLRDYLQ-----KH---------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGT-KRYIHRDLATRNILVE-NENRVKIGDFGLTKVL-PQ-D--KEYYKVKEPGESPIFWYAPESLT-ESK---FSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM-IVFHLIELLKNNGR----LPRPDGCP-------------------------------------------DEIYMIMTECWNNNVNQRPSFRDLA----LRV---------
--ITMKHKLGGGQYGEVYEGVWK-----KYSLTVAVKTLK-E-D-TM----EV--EE-FL-K-EAAVMKEI-K---HPNLVQLLGVCT--R-----EPPFY--IITEFMTYG--NLLDYLR-----EC--------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEK-KNFIHRDLAARNCLVG-ENHLVKVADFGLSRLM----T--GDTYTAHAGAKFPIKWTAPESLA-YNK---FSIKSDVWAFGVLLWEIATYGMSP---------------YPGI-DLSQVYELLEKDYR----MERPEGCP-------------------------------------------EKVYELMRACWQWNPSDRPSFAEIHQA--F-----------
--FEIGRPLGKGKFGNVYLAREK-----QSKFILALKVLFKA-QLEKA---GV--EH-QLRR-EVEIQSHL-R---HPNILRLYGYFH--D-----ATRVY--LILEYAPLG--TVYRELQ----KLS-----------------------------------------------------------------------------KFDEQRTATYITELANALSYCHS-KRVIHRDIKPENLLLG-SAGELKIADFGWSV------HAPSSRRTT---LCGTLDYLPPEMIE-GRM---HDEKVDLWSLGVLCYEFLV-GKPP---------------FEAN----TYQETYKRISR----VEFTFPDFVT-----------------------------------------EGARDLISRLLKHNPSQRPMLREVLEHPWI-----------
--FEIGRPLGKGKFGNVYLAREK-----KSHFIVALKVLFKS-Q-IEK---EG--VE-HQLRREIEIQAHL-H---HPNILRLYNYFY--D-----RRRIY--LILEYAPRG--ELYKELQ----KSC-----------------------------------------------------------------------------TFDEQRTATIMEELADALMYCHG-KKVIHRDIKPENLLLG-LKGELKIADFGWSV------HAPSLRRKT---MCGTLDYLPPEMIE-GRM---HNEKVDLWCIGVLCYELLV-GNPP---------------FESA----SHNETYRRIVK----VDLKFPASVP-----------------------------------------MGAQDLISKLLRHNPSERLPLAQVSAHPWV-----------
--ITVGQRIGSGSFGTVYKGKWH--------GDVAVKMLN-VTA-PT----PQQLQA-FK-N-EVGVLRKT-R---HVNILLFMGYST--------KPQLA--IVTQWCEGS--SLYHHLH-----II---------------------------------------------------------------------------ETKFEMIKLIDIARQTAQGMDYLHA-KSIIHRDLKSNNIFLH-EDLTVKIGDFGLATVK--S-R--WSGSHQFEQLSGSILWMAPEVIR-MQDKNPYSFQSDVYAFGIVLYELMT-GQLP---------------YSNINNRDQIIFMVGRGYLSPDLSKVRSNCP-------------------------------------------KAMKRLMAECLKKKRDERPLFPQILA---SI----------
--YTKIEKIGEGTYGVVYKGRHK-----TTGQVVAMKKIRLE-S-EEE---GV--PS-TAIR-EISLLKEL-R---HPNIVSLQDVLM--Q-----DSRLY--LIFEFLSM---DLKKYLDS-IPPGQ-----------------------------------------------------------------------------YMDSSLVKSYLYQILQGIVFCHS-RRVLHRDLKPQNLLID-DKGTIKLADFGLARAF----GIPIRVYTH---EVVTLWYRSPEVLLGSAR---YSTPVDIWSIGTIFAELAT-KKPL---------------FHGD-SEIDQLFRIFRALG----TPNNEVWPEVESLQDYKNTFPKWKP---------------GSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYF-----------
--FQKVEKIGEGTYGVVYKARNK-----LTGEVVALKKIRLD-T-ETE---GV--PS-TAIR-EISLLKEL-N---HPNIVKLLDVIH--T-----ENKLY--LVFEFLHQ---DLKKFMDA--SALT-----------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHS-HRVLHRDLKPQNLLIN-TEGAIKLADFGLARAF----GVPVRTYTH---EVVTLWYRAPEILLGCKY---YSTAVDIWSLGCIFAEMVT-RRAL---------------FPGD-SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR---------------QDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF-----------
-Y-EPVAEIGVGAYGTVYKARDP-----HSGHFVALKSVRVP-N-GGGGGGGL--PI-STVR-EVALLRRL-EAFEHPNVVRLMDVCA--TSRTDREIKVT--LVFEHVDQ---DLRTYLDK--APPP-----------------------------------------------------------------------------GLPAETIKDLMRQFLRGLDFLHA-NCIVHRDLKPENILVT-SGGTVKLADFGLARI-----YSYQMALTP---VVVTLWYRAPEVLL-QST---YATPVDMWSVGCIFAEMFR-RKPL---------------FCGN-SEADQLGKIFDLIG----LPPEDDWPRDVSLPRGAFPPRGPR-----------------PVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYL-----------
--YEKLEKIGEGTYGTVFKAKNR-----ETHEIVALKRVRLD-D-DDE---GV--PS-SALR-EICLLKEL-K---HKNIVRLHDVLH--S-----DKKLT--LVFEFCDQ---DLKKYFD---SCNG-----------------------------------------------------------------------------DLDPEIVKSFLFQLLKGLGFCHS-RNVLHRDLKPQNLLIN-RNGELKLADFGLARAF----GIPVRCYSA---EVVTLWYRPPDVLFGAKL---YSTSIDMWSAGCIFAELANAGRPL---------------FPGN-DVDDQLKRIFRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT---------------TSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYF-----------
-F-KKIKVLGSGAFGTVYKGLWIP-EGEKVKIPVAIKELR-E-A-TS----PKANKE-IL-D-EAYVMASV-D---NPHVCRLLGICL--------TSTVQ--LITQLMPFG--CLLDYVR-----EH---------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLED-RRLVHRDLAARNVLVK-TPQHVKITDFGLAKLL--G-A--EEKEYHAEGGKVPIKWMALESIL-HRI---YTHQSDVWSYGVTVWELMTFGSKP---------------YDGI-PASEISSILEKGER----LPQPPICT-------------------------------------------IDVYMIMVKCWMIDADSRPKFRELI----IEF---------
--YTDIKVIGNGSFGVVYQARLA-----ETRELVAIKKVLQD-K-RFK---NR----------ELQIMRKL-D---HCNIVRLRYFFY--SSG-EKKDELYLNLVLEYVPETVYRVARHFT---KAKL-----------------------------------------------------------------------------TIPILYVKVYMYQLFRSLAYIHS-QGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQ-----LVRGEPNVS---YICSRYYRAPELIFGATD---YTSSIDVWSAGCVLAELLL-GQPI---------------FPGD-SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH---------------PWTKVFKSRTPPEAIALCSSLLEYTPSSRLSPLEACAHSFF-----------
--YTDTKVIGNGSFGVVYQAKLC-----DSGELVAIKKVLQD-K-RFK---NR----------ELQIMRKL-D---HCNIVRLRYFFY--SSG-EKKDEVYLNLVLDYVPETVYRVA---RHYSRAKQ-----------------------------------------------------------------------------TLPVIYVKLYMYQLFRSLAYIHS-FGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQ-----LVRGEPNVS---YICSRYYRAPELIFGATD---YTSSIDVWSAGCVLAELLL-GQPI---------------FPGD-SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH---------------PWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFF-----------
--LKLEKKLGAGQFGEVWMATYN------KHTKVAVKTMK-P-G-SM----SV--EA-FL-A-EANVMKTL-Q---HDKLVKLHAVVT--------KEPIY--IITEFMAKG--SLLDFLK-----SD--------------------------------------------------------------------------EGSKQPLPKLIDFSAQIAEGMAFIEQ-RNYIHRDLRAANILVS-ASLVCKIADFGLARVI----E--DNEYTAREGAKFPIKWTAPEAIN-FGS---FTIKSDVWSFGILLMEIVTYGRIP---------------YPGM-SNPEVIRALERGYR----MPRPENCP-------------------------------------------EELYNIMMRCWKNRPEERPTFEYIQSV--L-----------
--ITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVN-E-A-AS----MRERIE-FL-N-EASVMKEF-N---CHHVVRLLGVVS--Q-----GQPTL--VIMELMTRG--DLKSYLR-----SLRPEM------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNA-NKFVHRDLAARNCMVA-EDFTVKIGDFGMTRDI--Y-E--TDYYRKGGKGLLPVRWMSPESLK-DGV---FTTYSDVWSFGVVLWEIATLAEQP---------------YQGL-SNEQVLRFVMEGGL----LDKPDNCP-------------------------------------------DMLFELMRMCWQYNPKMRPSFLEIISS--I-----------
--ITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVN-E-S-AS----LRERIE-FL-N-EASVMKGF-T---CHHVVRLLGVVS--K-----GQPTL--VVMELMAHG--DLKSYLR-----SLRPEA------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNA-KKFVHRDLAARNCMVA-HDFTVKIGDFGMTRDI--Y-E--TDYYRKGGKGLLPVRWMAPESLK-DGV---FTTSSDMWSFGVVLWEITSLAEQP---------------YQGL-SNEQVLKFVMDGGY----LDQPDNCP-------------------------------------------ERVTDLMRMCWQFNPKMRPTFLEIVNLL-------------
--LTFVQEIGSGQFGLVHLGYWL------NKDKVAIKTIR-E-G-AM----SE--ED-FI-E-EAEVMMKL-S---HPKLVQLYGVCL--E-----QAPIC--LVFEFMEHG--CLSDYLR-----TQ---------------------------------------------------------------------------RGLFAAETLLGMCLDVCEGMAYLEE-ACVIHRDLAARNCLVG-ENQVIKVSDFGMTRFV----L--DDQYTSSTGTKFPVKWASPEVFS-FSR---YSSKSDVWSFGVLMWEVFSEGKIP---------------YENR-SNSEVVEDISTGFR----LYKPRLAS-------------------------------------------THVYQIMNHCWKERPEDRPAFSRLLRQL-------------
-L-KYISQLGKGNFGSVELCRYDP-LGDNTGALVAVKQLQ-H-S-GP----DQ-QRD-FQ-R-EIQILKAL-H---SDFIVKYRGVSYGPG-----RQSLR--LVMEYLPSG--CLRDFLQ-----RH---------------------------------------------------------------------------RARLDASRLLLYSSQICKGMEYLGS-RRCVHRDLAARNILVE-SEAHVKIADFGLAKLL-PL-D--KDYYVVREPGQSPIFWYAPESLS-DNI---FSRQSDVWSFGVVLYELFTYCDKSCSPSAE--FLRMMGCERDVPALCRLLELLEEGQR----LPAPPACP-------------------------------------------AEVHELMKLCWAPSPQDRPSFSALGP---QL----------
--LSFGKTLGAGAFGKVVEATAYGLIKSDAAMTVAVKMLK-P-S-AH----LTEREA-LM-S-ELKVLSYLGN---HMNIVNLLGACT--I-----GGPTL--VITEYCCYG--DLLNFLR-----RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYMDMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLAS-KNCIHRDLAARNILLT-HGRITKICDFGLARDI--K-N--DSNYVVKGNARLPVKWMAPESIF-NCV---YTFESDVWSYGIFLWELFSLGSSP---------------YPGMPVDSKFYKMIKEGFR----MLSPEHAP-------------------------------------------AEMYDIMKTCWDADPLKRPTFKQIVQL--I-----------
--LKLVERLGAGQFGEVWMGYYN------GHTKVAVKSLK-Q-G-SM----SP--DA-FL-A-EANLMKQL-Q---HQRLVRLYAVVT--------QEPIY--IITEYMENG--SLVDFLK-----TP--------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEE-RNYIHRDLRAANILVS-DTLSCKIADFGLARLI----E--DNEYTAREGAKFPIKWTAPEAIN-YGT---FTIKSDVWSFGILLTEIVTHGRIP---------------YPGM-TNPEVIQNLERGYR----MVRPDNCP-------------------------------------------EELYQLMRLCWKERPEDRPTFDYLRSV--L-----------
--FEKISELGAGNGGVVFKVSHK-----PSGL-VMARKLIHL-E-IKP---AI--RN-QIIR-ELQVLHEC-N---SPYIVGFYGAFY--S-----DGEIS--ICMEHMDGG--SLDQVLK----KAG-----------------------------------------------------------------------------RIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVN-SRGEIKLCDFGVSG------QLIDSMANS---FVGTRSYMSPERLQ-GTH---YSVQSDIWSMGLSLVEMAV-GRYP---------------IPPP-DAKELELMFGCQVE----GDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFI-----------
IV--LKWELGEGAFGKVFLAECHNLLPEQDKMLVAVKALK-E-A-SE----SA-RQD-FQ-R-EAELLTML-Q---HQHIVRFFGVCT--E-----GRPLL--MVFEYMRHG--DLNRFLR-----SHGPDAKL-------------------------------------------------------------LAGGEDVAPGPLGLGQLLAVASQVAAGMVYLAG-LHFVHRDLATRNCLVG-QGLVVKIGDFGMSRDI--Y-S--TDYYRVGGRTMLPIRWMPPESIL-YRK---FTTESDVWSFGVVLWEIFTYGKQP---------------WYQL-SNTEAIDCITQGRE----LERPRACP-------------------------------------------PEVYAIMRGCWQREPQQRHSIKDVHARL-------------
-F-KFGKILGEGSFSTVVLAREL-----ATSREYAIKILEKR-H-IIK---EN--KVPYVTR-ERDVMSRL-D---HPFFVKLYFTFQ--D-----DEKLY--FGLSYAKNG--ELLKYIR----KIG-----------------------------------------------------------------------------SFDETCTRFYTAEIVSALEYLHG-KGIIHRDLKPENILLN-EDMHIQITDFGTAKVLS--PESKQARANS---FVGTAQYVSPELLT-EKS---ACKSSDLWALGCIIYQLVA-GLPP---------------FRAG----NEYLIFQKIIK----LEYDFPEKFF-----------------------------------------PKARDLVEKLLVLDATKRLGCEEM------EGYGPLKAHPF
VM--LSTRIGSGSFGTVYKGKWH--------GDVAVKILK-VVD-PT----PEQFQA-FR-N-EVAVLRKT-R---HVNILLFMGYMT--------KDNLA--IVTQWCEGS--SLYKHLH-----VQ---------------------------------------------------------------------------ETKFQMFQLIDIARQTAQGMDYLHA-KNIIHRDMKSNNIFLH-EGLTVKIGDFGLATVK--S-R--WSGSQQVEQPTGSVLWMAPEVIR-MQDNNPFSFQSDVYSYGIVLYELMT-GELP---------------YSHINNRDQIIFMVGRGYASPDLSKLYKNCP-------------------------------------------KAMKRLVADCVKKVKEERPLFPQILSS--I-----------
-L-RLEVKLGQGCFGEVWMGTWN------GTTRVAIKTLK-P-G-TM----SP--EA-FL-Q-EAQVMKKL-R---HEKLVQLYAVVS--------EEPIY--IVTEYMSKG--SLLDFLK-----GE--------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVER-MNYVHRDLRAANILVG-ENLVCKVADFGLARLI----E--DNEYTARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELTTKGRVP---------------YPGM-VNREVLDQVERGYR----MPCPPECP-------------------------------------------ESLHDLMCQCWRKEPEERPTFEYLQAF--L-----------
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