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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
AGC RPS6KA2 203
Other AURKA 133
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK4 6
CMGC CDK5 4
CMGC CDK9 2.7.11.23
CAMK CHEK1 9
CAMK CHEK2 220
Other CHUK 15
CMGC MAPK1 13
CMGC MAPK14 24
TK NTRK3 538
STE PAK4 321
CMGC PCTK1 165
CAMK PIM1 38
Other PLK1 53
Other PLK3 62
AGC PRKCA 339
AGC PRKCD 349
TK SRC 270
----FELLKVLGQGSYGKVFLVRKV-----KGSDAGQLYAMKVLKKAT-LKV---RD-RVRSKM------ERDILAEV-N---HPF--IVKLHYAFQT--------EGKLYLILDFLRGG-DLFTRLS---KEV-----------------MFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLD-EEGHI---KITDFGLSKEA-----IDHDKRA--------------YS---FCGTIEYMAPEVVN---RRGHTQSADWWSFGVLMFEMLT-GSLPFQGK--------------DRKETMALILKAKLGMPQFLS-----------------------------GEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFF--------------
----FEIGRPLGKGKFGNVYLAREK-----QSKF---ILALKVLFKAQLEKA---GV-EHQLRR------EVEIQSHL-R---HPN--ILRLYGYFHD--------ATRVYLILEYAPLG-TVYRELQ---KLS-----------------KFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLG-SAGEL---KIADFGWSV-------HAPSSRR--------------TT---LCGTLDYLPPEMIE---GRMHDEKVDLWSLGVLCYEFLV-GKPPFEAN--------------TYQETYKRISRVEFTFPDFVT-----------------------------EGARDLISRLLKHNPSQRPM-----LREVLEHPWI--------------
----YTKIEKIGEGTYGVVYKGRHK-----TTGQ---VVAMKKIRLES-EEE---GV-PSTAIR------EISLLKEL-R---HPN--IVSLQDVLMQ--------DSRLYLIFEFLSM--DLKKYLDSIPPGQ-----------------YMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLID-DKGTI---KLADFGLARAF-----GIPIRVY--------------TH---EVVTLWYRSPEVLL--GSARYSTPVDIWSIGTIFAELAT-KKPLFHGD-----------SEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKP---GSLASHVKNLDENGLDLLSKMLIYDPAKRIS-----GKMALNHPYF--------------
----FQKVEKIGEGTYGVVYKARNK-----LTGE---VVALKKIRLDT-ETE---GV-PSTAIR------EISLLKEL-N---HPN--IVKLLDVIHT--------ENKLYLVFEFLHQ--DLKKFMDA-SALT-----------------GIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLIN-TEGAI---KLADFGLARAF-----GVPVRTY--------------TH---EVVTLWYRAPEILL--GCKYYSTAVDIWSLGCIFAEMVT-RRALFPGD-----------SEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWAR---QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-----AKAALAHPFF--------------
---Y-EPVAEIGVGAYGTVYKARDP-----HSGH---FVALKSVRVPN-GGGGGGGL-PISTVR------EVALLRRL-EAFEHPN--VVRLMDVCATSR---TDREIKVTLVFEHVDQ--DLRTYLDK-APPP-----------------GLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVT-SGGTV---KLADFGLARI------YSYQMAL--------------TP---VVVTLWYRAPEVLL---QSTYATPVDMWSVGCIFAEMFR-RKPLFCGN-----------SEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPR-----PVQSVVPEMEESGAQLLLEMLTFNPHKRIS-----AFRALQHSYL--------------
----YEKLEKIGEGTYGTVFKAKNR-----ETHE---IVALKRVRLDD-DDE---GV-PSSALR------EICLLKEL-K---HKN--IVRLHDVLHS--------DKKLTLVFEFCDQ--DLKKYFD--SCNG-----------------DLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLIN-RNGEL---KLADFGLARAF-----GIPVRCY--------------SA---EVVTLWYRPPDVLF--GAKLYSTSIDMWSAGCIFAELANAGRPLFPGN-----------DVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPAT---TSLVNVVPKLNATGRDLLQNLLKCNPVQRIS-----AEEALQHPYF--------------
----YEKLAKIGQGTFGEVFKARHR-----KTGQ---KVALKKVLMEN-EKE---GF-PITALR------EIKILQLL-K---HEN--VVNLIEICRTKASPYNRCKGSIYLVFDFCEH--DLAGLLS--NVLV-----------------KFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLIT-RDGVL---KLADFGLARAFSL-AKNSQPNRY--------------TN---RVVTLWYRPPELLL--GERDYGPPIDLWGAGCIMAEMWT-RSPIMQGN-----------TEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRID-----SDDALNHDFF--------------
---W-DLVQTLGEGAYGEVQLAVNR-----VTEE---AVAVKIVDMKR-AVD---CP-ENI-KK------EICINKML-N---HEN--VVKFYGHRRE--------GNIQYLFLEYCSGG-ELFDRIE---PDI-----------------GMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLD-ERDNL---KISDFGLATVFR---YNNRERLL--------------NK---MCGTLPYVAPELLK--RREFHAEPVDVWSCGIVLTAMLA-GELPWDQP-------------SDSCQEYSDWKEKKTYLNPWKKI--D-------------------------SAPLALLHKILVENPSARIT-----IPDIKKDRW-Y-------------
--YI--MSKTLGSGACGEVKLAFER-----KTCK---KVAIKIISKRK-FAI---GS-AREADPALNVETEIEILKKL-N---HPC--IIKIKNFF-D--------AEDYYIVLELMEGG-ELFDKVV---GNK-----------------RLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLS-SQEEDCLIKITDFGHSKI------LGETSLM--------------RT---LCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLS-GYPPFSEH-------------RTQVSLKDQITSGKYNFIPEVWAEVS-------------------------EKALDLVKKLLVVDPKARFT-----TEEALRHPWL--------------
---W-EMRERLGTGGFGNVCLYQHR-----ELDL---KIAIKSCRLEL-STK---NR-ER-WCH------EIQIMKKL-N---HAN--VVKACDVPEELN---ILIHDVPLLAMEYCSGG-DLRKLLNKPENCC-----------------GLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQ-DVGGKIIHKIIDLGYAKD------VDQGSLC--------------TS---FVGTLQYLAPELFE---NKPYTATVDYWSFGTMVFECIA-GYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCS-------------------LVVEPMENWLQLMLNWDPQQRG----------------GPVDLTLKQ-----
----YTNLSYIGEGAYGMVCSAYDN-----VNKV---RVAIKKISPFE-HQT---YC-QRT-LR------EIKILLRF-R---HEN--IIGINDIIRAPT---IEQMKDVYIVQDLMET--DLYKLLK----TQ-----------------HLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLN-TTCDL---KICDFGLARVAD--PDHDHTGFL--------------TE---YVATRWYRAPEIML--NSKGYTKSIDIWSVGCILAEMLS-NRPIFPGK-----------HYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKN-KVPWNRLFPNADSKALDLLDKMLTFNPHKRIE-----VEQALAHPYL--------------
----YQNLSPVGSGAYGSVCAAFDT-----KTGL---RVAVKKLSRPF-QSI---IH-AKRTYR------ELRLLKHM-K---HEN--VIGLLDVFTPARS--LEEFNDVYLVTHLMGA--DLNNIVK----CQ-----------------KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVN-EDCEL---KILDFGLAR--------HTDDEM--------------TG---YVATRWYRAPEIML--NWMHYNQTVDIWSVGCIMAELLT-GRTLFPGT-----------DHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMP-KMNFANVFIGANPLAVDLLEKMLVLDSDKRIT-----AAQALAHAYF--------------
---I-VLKRELGEGAFGKVFLAECYNLSPTKDKM---LVAVKALK-DP-TL----AARKDFQ-R------EAELLTNL-Q---HEH--IVKFYGVCGD--------GDPLIMVFEYMKHG-DLNKFLR----AHGPDAMILVDGQPRQAKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVG-ANLLV---KIGDFGMSRDV--Y--S--TDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIM---YRKFTTESDVWSFGVILWEIFTYGKQPWFQL-----------SNTEVIECITQGRVLERPRVCP-------------------------------KEVYDVMLGCWQREPQQRLN-----IKEIYKIL----------------
DNFI-----KIGEGSTGIVCIATVR-----SSGK---LVAVKKMDLRK-QQR---RE-L--LFN------EVVIMRDY-Q---HEN--VVEMYNSYLV--------GDELWVVMEFLEGG-ALTDIVT----HT-----------------RMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLT-HDGRV---KLSDFGFCAQV-----SKEVPRR--------------KS---LVGTPYWMAPELIS---RLPYGPEVDIWSLGIMVIEMVD-GEPPYFNE-----------PPLKAMKMIRDNLPPRLKNLHKVS-----------------------------PSLKGFLDRLLVRDPAQRAT-----AAELLKHPFL--------------
---Y-IKLDKLGEGTYATVYKGKSK-----LTDN---LVALKEIRLEH-EEG---AP-C-TAIR------EVSLLKDL-K---HAN--IVTLHDIIHT--------EKSLTLVFEYLDK--DLKQYLD--DCGN-----------------IINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLIN-ERGEL---KLADFGLARAK-----SIPTKTY--------------SN---EVVTLWYRPPDILL--GSTDYSTQIDMWGVGCIFYEMAT-GRPLFPGS-----------TVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYR--AEALLSHAPRLDSDGADLLTKLLQFEGRNRIS-----AEDAMKHPFF--------------
----YQVGPLLGSGGFGSVYSGIRV-----SDNL---PVAIKHVEKDR-ISD---WG-ELPNGTRV--PMEVVLLKKV-S---SGFSGVIRLLDWFER--------PDSFVLILERPEPVQDLFDFIT---ERG-----------------ALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGEL---KLIDFGSGA-------LLKDTVY--------------TD---FDGTRVYSPPEWIR--YHRYHGRSAAVWSLGILLYDMVC-GDIPFEHD--------------------EEIIRGQVFFRQRVS-----------------------------SECQHLIRWCLALRPSDRPT-----FEEIQNHPWM--------------
--YV--RGRFLGKGGFAKCFEISDA-----DTKE---VFAGKIVPKSL-LLK---PH-QREKMS-----MEISIHRSL-A---HQH--VVGFHGFFED--------NDFVFVVLELCRRR-SLLELHK---RRK-----------------ALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLN-EDLEV---KIGDFGLATKV-----EYDGERK--------------KT---LCGTPNYIAPEVLS---KKGHSFEVDVWSIGCIMYTLLV-GKPPFETS--------------CLKETYLRIKKNEYSIPKHIN-----------------------------PVAASLIQKMLQTDPTARPT-----INELLNDEFF--------------
--YL--KGRLLGKGGFARCYEATDT-----ETGS---AYAVKVIPQSR-VAK---PH-QREKIL-----NEIELHRDL-Q---HRH--IVRFSHHFED--------ADNIYIFLELCSRK-SLAHIWK---ARH-----------------TLLEPEVRYYLRQILSGLKYLHQRGILHRDLKLGNFFIT-ENMEL---KVGDFGLAARL-----EPPEQRK--------------KT---ICGTPNYVAPEVLL---RQGHGPEADVWSLGCVMYTLLC-GSPPFETA--------------DLKETYRCIKQVHYTLPASLS-----------------------------LPARQLLAAILRASPRDRPS-----IDQILRHDFF--------------
---F-NFLMVLGKGSFGKVMLADRK-----GTEE---LYAIKILKKDV-VIQ---DD-DVECTM-----VEKRVLALLDK---PPF--LTQLHSCFQT--------VDRLYFVMEYVNGG-DLMYHIQ---QVG-----------------KFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLD-SEGHI---KIADFGMCKEH-----MMDGVTT--------------RT---FCGTPDYIAPEIIA---YQPYGKSVDWWAYGVLLYEMLA-GQPPFDGE--------------DEDELFQSIMEHNVSYPKSLS-----------------------------KEAVSVCKGLMTKHPAKRLG-----C---------GPEGERDVREHAFF
--FI--FHKVLGKGSFGKVLLGELK-----GRGE---YFAIKALKKDV-VLI---DD-DVECTM-----VEKRVLTLAAE---NPF--LTHLICTFQT--------KDHLFFVMEFLNGG-DLMYHIQ---DKG-----------------RFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLD-RDGHI---KIADFGMCKEN-----IFGESRA--------------ST---FCGTPDYIAPEILQ---GLKYTFSVDWWSFGVLLYEMLI-GQSPFHGD--------------DEDELFESIRVDTPHYPRWIT-----------------------------KESKDILEKLFEREPTKRLG----VTGNIKIHPFF--------------
---L-RLEVKLGQGCFGEVWMGTWN------GTT---RVAIKTLK-PG-TM----SP-EAFL-Q------EAQVMKKL-R---HEK--LVQLYAVVS---------EEPIYIVTEYMSKG-SLLDFLK----GE--------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVG-ENLVC---KVADFGLARLI-----E--DNEY--------------TARQGAKFPIKWTAPEAAL---YGRFTIKSDVWSFGILLTELTTKGRVPYPGM-----------VNREVLDQVERGYRMPCPPECP-------------------------------ESLHDLMCQCWRKEPEERPT-----FEYLQAF--L--------------
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