Protein-Protein Interaction Search

Input an interacting protein pair as a query to search its homologous interactions across multiple species

Press the to obtain more information on that specific field.

 

Query protein pair (sequences in FASTA format or UniProt ID) :
Input sequences in FASTA format
	Interacting partner 1:	>sp|P61967|AP1S1_MOUSE MMRFMLLFSRQGKLRLQKWYLATSDKERKKMVRELMQVVLARKPKMCSFLEWRDLKVVYK RYASLYFCCAIEGQDNELITLELIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLMG GDVQDTSKKSVLKAIEQADLLQEEDESPRSVLEEMGLA
	Interacting partner 2:	>sp|P22892|AP1G1_MOUSE MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLG YPAHFGQLECLKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQG LALCTLGCMGSSEMCRDLAGEVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATK NLLNEKNHGVLHTSVVLLTEMCERSPDMLAHFRKLVPQLVRILKNLIMSGYSPEHDVSGI SDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIK SESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVS IKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHID TIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCI GEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCT VNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQ TNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPASAGGELLDLL GDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLFNDIAPGIPSITAYSKNGLKIEFT FERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSVVPAFNTGTITQ VIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ
Input UniProt ID (Ex: AP1S1_MOUSE)
	Interacting partner 1:              Interacting partner 2:
Options:
	E-value cut-off threshold for homolog searching
	10 10-1 10-10 (Default) Other:  (Ex: -50 = 10-50)
	Joint E-value
	10-100 10-40 (Default) 10-10 Other:  (Ex: -50 = 10-50)
	Number of homologous interactions in each species ( Ranking by Joint E-value )
	Best-match Three All (Default) Other:
	The conservation ratio (CRD) of a domain-domain pair
	1.00 All 0.60 (Default) Other:  (Input Range: 0.01 ~ 0.99)
	The conservation ratio (CRF) of a molecular function term pair
	1.00 All 0.60 (Default) Other:  (Input Range: 0.01 ~ 0.99)
	Choose output species
	Homo sapiens Caenorhabditis elegans Others (Taxonomy ID) Ex: 9913;9598;...        Drosophila melanogaster Arabidopsis thaliana Saccharomyces cerevisiae Mus musculus Rattus norvegicus All (Default)
Reference to cite:
	Chen, C.-C., Lin, C.-Y., Lo, Y.-S. and Yang, J.-M. PPISearch: a web server for searching homologous protein-protein interactions across multiple species. Nucleic Acids Research 37:W376-W383 (2009).
Related references:
	1. Chen, Y.-C., Lo, Y.-S., Hsu, W.-C. and Yang, J.-M. 3D-partner: a web server to infer interacting partners and binding models. Nucleic Acids Research 35:W561-W567 (2007).
	2. Lo, Y.-S., Lin, C.-Y. and Yang, J.-M. PCFamily: a web server for searching homologous protein complexes. Nucleic Acids Research 38:W516-W522 (2010).
	3. Chen, Y.-F., Hsu, K.-C., Lin, S.-R., Wang, W.-C., Huang, Y.-C. and Yang, J.-M. SiMMap: a web server for inferring site-moiety map to recognize interaction preferences between protein pockets and compound moieties. Nucleic Acids Research 38:W424-W430 (2011).
	4. Liu, I.-H., Lo, Y.-S. and Yang, J.-M. PAComplex: a web server to infer peptide antigen families and binding models from TCR–pMHC complexes. Nucleic Acids Research 39:W254-W260 (2011).
Last updated 2012/01/17