|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TKL BRAF 203
TK ABL1 261
TK ABL2 288
TK DDR1 572
TK EPHA1 624
TK EPHA3 621
TK FLT1 827
TK FLT4 845
TK KDR 834
TK KIT 589
TK LCK 245
TKL LIMK1 339
TKL LIMK2 331
TK MUSK 575
TK NTRK2 538
TK NTRK3 538
TK PDGFRB 600
TK TEK 824
TK TIE1 839
--ITVGQRIGSGSFGTVYKGKWH-------------------GDVAV-KMLNVTAPTPQQLQAFKNEVGVLRKT-RHVNILLFMGYST--KPQLAIVTQWCEGSSLYHHLHII--------------------------------------------------------------------------------------------------ETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVK-------------SRWSGS--------------HQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMT-GQ-LPYSNINNRDQIIFMV----GRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILA--SI
--ITMKHKLGGGQYGEVYEGVWK----------------KYSLTVAV-KTLKE-DTMEV--EEFLKEAAVMKEI-KHPNLVQLLGVCT-REPPFYIITEFMTYGNLLDYLREC-------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLM--------------TGDTY--------------TAHAGAKFPIKWTAPESLAYNK---FSIKSDVWAFGVLLWEIATYGM-SPYPGI-DLSQVYELL----EKDYR----MERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQA-F-
--ITMKHKLGGGQYGEVYVGVWK----------------KYSLTVAV-KTLKE-DTMEV--EEFLKEAAVMKEI-KHPNLVQLLGVCT-LEPPFYIVTEYMPYGNLLDYLREC-------------------------------------------------------------------------------------------------NREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLM--------------TGDTY--------------TAHAGAKFPIKWTAPESLAYNT---FSIKSDVWAFGVLLWEIATYGM-SPYPGI-DLSQVYDLL----EKGYR----MEQPEGCPPKVYELMRACWKWSPADRPSFAETHQA-F-
--LRFKEKLGEGQFGEVHLCEVDSPQDLVSLDFPLNVRKGHPLLVAV-KILRP-DATKNARNDFLKEVKIMSRL-KDPNIIRLLGVCV-QDDPLCMITDYMENGDLNQFLSAHQLEDKAAE--------------------------------------------------------------------------------GAPGDGQAAQGPTISYPMLLHVAAQIASGMRYLATLNFVHRDLATRNCLVGENFTIKIADFGMSRNL-------------YAGDYY--------------RVQGRAVLPIRWMAWECILMGK---FTTASDVWAFGVTLWEVLMLCRAQPFGQL-TDEQVIENAGEFFRDQGRQ-VYLSRPPACPQGLYELMLRCWSRESEQRPPFSQLHRFL--
LM--VDTVIGEGEFGEVYRGTLR-------------LPSQDCKTVAI-KTLKD-TSPGGQWWNFLREATIMGQF-SHPHILHLEGVVT-KRKPIMIITEFMENGALDAFLRER--------------------------------------------------------------------------------------------------EDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLL-------------DDFDGT--------------YETQGGKIPIRWTAPEAIAHRI---FTTASDVWSFGIVMWEVLSFGD-KPYGEM-SNQEVMKSI----EDGYR----LPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHL--
-I-SIDKVVGAGEFGEVCSGRLK-------------LPSKKEISVAI-KTLKV-GYTEKQRRDFLGEASIMGQF-DHPNIIRLEGVVT-KSKPVMIVTEYMENGSLDSFLRKH--------------------------------------------------------------------------------------------------DAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVL-------------EDDPEA-------------AYTTRGGKIPIRWTSPEAIAYRK---FTSASDVWSYGIVLWEVMSYGE-RPYWEM-SNQDVIKAV----DEGYR----LPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSIL--
--LKLGKSLGRGAFGKVVQASAFG-----------IKKSPTCRTVAV-KMLKE-GATASEYKALMTELKILTHIGHHLNVVNLLGACTKQGGPLMVIVEYCKYGNLSNYLKSKRDLFFLNKDAALHMEPKKEKMEPGLEQGKKPR--------------------------------LDSVTSSESFASSGFQEDKSLSDVEEEEDSDGFYKEPITMEDLISYSFQVARGMEFLSSRKCIHRDLAARNILLSENNVVKICDFGLARDI-------------YKNPDY--------------VRKGDTRLPLKWMAPESIFDKI---YSTKSDVWSYGVLLWEIFSLGG-SPYPGVQMDEDFCSRL----REGMR----MRAPEYSTPEIYQIMLDCWHRDPKERPRFAELVEKL--
--LHLGRVLGYGAFGKVVEASAFG-----------IHKGSSCDTVAV-KMLKE-GATASEHRALMSELKILIHIGNHLNVVNLLGACTKPQGPLMVIVEFCKYGNLSNFLRAKRDAFSPCAEKSPEQRGRFRAMVELARLDRRR-----------------------------------PGSSDRVLFARFSKTEGGARRASPDQEAEDLWLSPLTMEDLVCYSFQVARGMEFLASRKCIHRDLAARNILLSESDVVKICDFGLARDI-------------YKDPDY--------------VRKGSARLPLKWMAPESIFDKV---YTTQSDVWSFGVLLWEIFSLGA-SPYPGVQINEEFCQRL----RDGTR----MRAPELATPAIRRIMLNCWSGDPKARPAFSELVEIL--
--LKLGKPLGRGAFGQVIEADAFG-----------IDKTATCRTVAV-KMLKE-GATHSEHRALMSELKILIHIGHHLNVVNLLGACTKPGGPLMVIVEFCKFGNLSTYLRSKRNEFVPYKTKGARFRQGKDYVGAIPVDLKRRL---------------------------------DSITSSQSSASSGFVEEKSLSDVEEEEAPEDLYKDFLTLEHLICYSFQVAKGMEFLASRKCIHRDLAARNILLSEKNVVKICDFGLARDI-------------YKDPDY--------------VRKGDARLPLKWMAPETIFDRV---YTIQSDVWSFGVLLWEIFSLGA-SPYPGVKIDEEFCRRL----KEGTR----MRAPDYTTPEMYQTMLDCWHGEPSQRPTFSELVEHL--
--LSFGKTLGAGAFGKVVEATAYG-----------LIKSDAAMTVAV-KMLKP-SAHLTEREALMSELKVLSYLGNHMNIVNLLGACT-IGGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM-----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTHGRITKICDFGLARDI-------------KNDSNY--------------VVKGNARLPVKWMAPESIFNCV---YTFESDVWSYGIFLWELFSLGS-SPYPGMPVDSKFYKMI----KEGFR----MLSPEHAPAEMYDIMKTCWDADPLKRPTFKQIVQL-I-
--LKLVERLGAGQFGEVWMGYYN-----------------GHTKVAV-KSLKQ-GSMSP--DAFLAEANLMKQL-QHQRLVRLYAVVT--QEPIYIITEYMENGSLVDFLKTP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLI--------------EDNEY--------------TAREGAKFPIKWTAPEAINYGT---FTIKSDVWSFGILLTEIVTHGR-IPYPGM-TNPEVIQNL----ERGYR----MVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSV-L-
LI--HGEVLGKGCFGQAIKVTHR----------------ETGEVMVM-KELI--RFDEETQRTFLKEVKVMRCL-EHPNVLKFIGVLY-KDKRLNFITEYIKGGTLRGIIKSM--------------------------------------------------------------------------------------------------DSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLM-------------VDEKTQPEGLRS--LKKPDRKKRYTVVGNPYWMAPEMINGRS---YDEKVDVFSFGIVLCEIIGRVN-ADPDYLPRTMDFGLNV----RGFLD----RYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEH--WL
LI--HGEVLGKGFFGQAIKVTHK-----------------ATGKVMVMKEL-I-RCDEETQKTFLTEVKVMRSL-DHPNVLKFIGVLY-KDKKLNLLTEYIEGGTLKDFLRSM---------------------------------------------------------------------------------------------------DPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATT----KKRTL-----RKNDRKKRYTVVGNPYWMAPEMLNGKS---YDETVDIFSFGIVLCEIIGQVY-ADPDCLPRTLDFGLNV----KLFWE----KFVPTDCPPAFFPLAAICCRLEPESRPAFSKLED--SF
-I-EYVRDIGEGAFGRVFQARAPG-----------LLPYEPFTMVAV-KMLKE-EASADMQADFQREAALMAEF-DNPNIVKLLGVCA-VGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHS---------------------------------------------------------------------------DLSMRAQVSSPGPPPLSCAEQLCIARQVAAGMAYLSERKFVHRDLATRNCLVGENMVVKIADFGLSRNI-------------YSADYY--------------KANENDAIPIRWMPPESIFYNR---YTTESDVWAYGVVLWEIFSYGL-QPYYGM-AHEEVIYYV----RDGNI----LSCPENCPVELYNLMRLCWSKLPADRPSFTSIHRIL--
-I-VLKRELGEGAFGKVFLAECYN-----------LCPEQDKILVAV-KTLKD-ASDNA-RKDFHREAELLTNL-QHEHIVKFYGVCV-EGDPLIMVFEYMKHGDLNKFLRAHGPDAV--------------------------------------------------------------------------------------LMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDV-------------YSTDYY--------------RVGGHTMLPIRWMPPESIMYRK---FTTESDVWSLGVVLWEIFTYGK-QPWYQL-SNNEVIECI----TQGRV----LQRPRTCPQEVYELMLGCWQREPHMRKNIKGIHTL-L-
-I-VLKRELGEGAFGKVFLAECYN-----------LSPTKDKMLVAV-KALKD-PTLAA-RKDFQREAELLTNL-QHEHIVKFYGVCG-DGDPLIMVFEYMKHGDLNKFLRAHGPDAMIL-----------------------------------------------------------------------------------VDGQPRQAKGELGLSQMLHIASQIASGMVYLASQHFVHRDLATRNCLVGANLLVKIGDFGMSRDV-------------YSTDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIMYRK---FTTESDVWSFGVILWEIFTYGK-QPWFQL-SNTEVIECI----TQGRV----LERPRVCPKEVYDVMLGCWQREPQQRLNIKEIYKIL--
-L-VLGRTLGSGAFGQVVEATAHG-----------LSHSQATMKVAV-KMLKS-TARSSEKQALMSELKIMSHLGPHLNVVNLLGACT-KGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDI-------------MRDSNY--------------ISKGSTFLPLKWMAPESIFNSL---YTTLSDVWSFGILLWEIFTLGG-TPYPELPMNEQFYNAI----KRGYR----MAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVL--LL
--IKFQDVIGEGNFGQVLKARIK--------------KDGLRMDAAI-KRMKE-YASKDDHRDFAGELEVLCKLGHHPNIINLLGACE-HRGYLYLAIEYAPHGNLLDFLRKSRVLETDP-----------------------------------------------------------------------------------AFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQ----------------EVY--------------VKKTMGRLPVRWMAIESLNYSV---YTTNSDVWSYGVLLWEIVSLGG-TPYCGM-TCAELYEKL----PQGYR----LEKPLNCDDEVYDLMRQCWREKPYERPSFAQILV--SL
--ITFEDLIGEGNFGQVIRAMIK--------------KDGLKMNAAI-KMLKE-YASENDHRDFAGELEVLCKLGHHPNIINLLGACK-NRGYLYIAIEYAPYGNLLDFLRKSRVLETDP-----------------------------------------------------------------------------------AFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGE----------------EVY--------------VKKTMGRLPVRWMAIESLNYSV---YTTKSDVWSFGVLLWEIVSLGG-TPYCGM-TCAELYEKL----PQGYR----MEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQ-L-
|