|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK EPHA1 203
TK ABL1 261
TK BMX 422
TK BTK 402
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK4 6
TK CSK 195
TK EGFR 712
TK EPHB1 619
TK EPHB2 621
TK EPHB3 633
TK EPHB4 615
TK FGFR1 478
TK FGFR2 481
TK FYN 271
TK JAK2 849
TK JAK3 822
TK KDR 834
TK LCK 245
TKL LIMK1 339
CMGC MAPK14 24
TK PTK6 191
TK RET 724
TK SRC 270
TK TEK 824
TK TNK2 150
TK TXK 271
TK YES1 277
LM--VDTVIGEGEFGEVYRGTLR----LPSQDCKTVAIKTLK-D--TSP----GGQWWNFL-REATIMGQF-S---HPHILHLEGVVT--K------RKPIMIITEFMENGALDAFLR----ER-----------------------------------------------------------------EDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLL---DD--FDGT------------YETQGGKIPIRWTAPEAIA-HRIFTTASDVWSFGIVMWEVLSFGDKP---------------YGEM-SNQEVMKSI-EDGYRLPPPVDCP------------------------------APLYELMKNCWAYDRARRPHFQKLQAHL----
--ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-E--DTM----EV--EEFL-KEAAVMKEI-K---HPNLVQLLGVCT--R------EPPFYIITEFMTYGNLLDYLR----EC----------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLM----T--GDTY------------TAHAGAKFPIKWTAPESLA-YNKFSIKSDVWAFGVLLWEIATYGMSP---------------YPGI-DLSQVYELL-EKDYRMERPEGCP------------------------------EKVYELMRACWQWNPSDRPSFAEIHQA--F--
--ITLLKELGSGQFGVVQLGKWK--------GQYDVAVKMIK-E--GSM----SE--DEFF-QEAQTMMKL-S---HPKLVKFYGVCS--K------EYPIYIVTEYISNGCLLNYLR----SH-----------------------------------------------------------------GKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYV----L--DDQY------------VSSVGTKFPVKWSAPEVFH-YFKYSSKSDVWAFGILMWEVFSLGKQP---------------YDLY-DNSQVVLKV-SQGHRLYRPHLAS------------------------------DTIYQIMYSCWHELPEKRPTFQQLLSS--I--
--LTFLKELGTGQFGVVKYGKWR--------GQYDVAIKMIK-E--GSM----SE--DEFI-EEAKVMMNL-S---HEKLVQLYGVCT--K------QRPIFIITEYMANGCLLNYLR----EM-----------------------------------------------------------------RHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYV----L--DDEY------------TSSVGSKFPVRWSPPEVLM-YSKFSSKSDIWAFGVLMWEIYSLGKMP---------------YERF-TNSETAEHI-AQGLRLYRPHLAS------------------------------EKVYTIMYSCWHEKADERPTFKILLSN--I--
--YTKIEKIGEGTYGVVYKGRHK-------TTGQVVAMKKIRLE--SEEE---GV--PSTAIREISLLKEL-R---HPNIVSLQDVLM--Q------DSRLYLIFEFLSM-DLKKYLDSIPPGQ-------------------------------------------------------------------YMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAF----GIPIRVY------------TH---EVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELAT-KKPL---------------FHGD-SEIDQLFRI-FRALGTPNNEVWPEVESLQDYKNTFPKWKP--GSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYF--
--FQKVEKIGEGTYGVVYKARNK-------LTGEVVALKKIRLD--TETE---GV--PSTAIREISLLKEL-N---HPNIVKLLDVIH--T------ENKLYLVFEFLHQ-DLKKFMDA-SALT-------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAF----GVPVRTY------------TH---EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVT-RRAL---------------FPGD-SEIDQLFRI-FRTLGTPDEVVWPGVTSMPDYKPSFPKWAR--QDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF--
-Y-EPVAEIGVGAYGTVYKARDP-------HSGHFVALKSVRVP--NGGGGGGGL--PISTVREVALLRRL-EAFEHPNVVRLMDVCA--TSR-TDREIKVTLVFEHVDQ-DLRTYLDK-APPP-------------------------------------------------------------------GLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARI-----YSYQMAL------------TP---VVVTLWYRAPEVLL-QSTYATPVDMWSVGCIFAEMFR-RKPL---------------FCGN-SEADQLGKI-FDLIGLPPEDDWPRDVSLPRGAFPPRGPR----PVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYL--
--LKLLQTIGKGEFGDVMLGDYR---------GNKVAVKCIK-N--DAT----AQ---AFL-AEASVMTQL-R---HSNLVQLLGVIV-EE------KGGLYIVTEYMAKGSLVDYLR----SR----------------------------------------------------------------GRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEA----------S------------STQDTGKLPVKWTAPEALR-EKKFSTKSDVWSFGILLWEIYSFGRVP---------------YPRI-PLKDVVPRV-EKGYKMDAPDGCP------------------------------PAVYEVMKNCWHLDAAMRPSFLQLREQL----
-F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E--ATS----PKANKEIL-DEAYVMASV-D---NPHVCRLLGICL---------TSTVQLITQLMPFGCLLDYVR----EH-----------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLL---GA--EEKE------------YHAEGGKVPIKWMALESIL-HRIYTHQSDVWSYGVTVWELMTFGSKP---------------YDGI-PASEISSIL-EKGERLPQPPICT------------------------------IDVYMIMVKCWMIDADSRPKFRELI----IEF
-V-KIEEVIGAGEFGEVYKGRLK----LPGKREIYVAIKTLK-A--GYS----EKQRRDFL-SEASIMGQF-D---HPNIIRLEGVVT--K------SRPVMIITEFMENGALDSFLR----QN-----------------------------------------------------------------DGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTS--DPTY------------TSSLGGKIPVRWTAPEAIA-YRKFTSASDVWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAI-EQDYRLPPPMDCP------------------------------AALHQLMLDCWQKDRNSRPRFAEIVNTL----
-V-KIEQVIGAGEFGEVCSGHLK----LPGKREIFVAIKTLK-S--GYT----EKQRRDFL-SEASIMGQF-D---HPNVIHLEGVVT--K------STPVMIITEFMENGSLDSFLR----QN-----------------------------------------------------------------DGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTS--DPTY------------TSALGGKIPIRWTAPEAIQ-YRKFTSASDVWSYGIVMWEVMSYGERP---------------YWDM-TNQDVINAI-EQDYRLPPPMDCP------------------------------SALHQLMLDCWQKDRNHRPKFGQIVNTL----
-V-KIEEVIGAGEFGEVCRGRLK----QPGRREVFVAIKTLK-V--GYT----ERQRRDFL-SEASIMGQF-D---HPNIIRLEGVVT--K------SRPVMILTEFMENCALDSFLR----LN-----------------------------------------------------------------DGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDPS--DPTY------------TSSLGGKIPIRWTAPEAIA-YRKFTSASDVWSYGIVMWEVMSYGERP---------------YWDM-SNQDVINAV-EQDYRLPPPMDCP------------------------------TALHQLMLDCWVRDRNLRPKFSQIVNTL----
-V-KIEEVIGAGEFGEVCRGRLK----APGKKESCVAIKTLK-G--GYT----ERQRREFL-SEASIMGQF-E---HPNIIRLEGVVT--N------SMPVMILTEFMENGALDSFLR----LN-----------------------------------------------------------------DGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSS--DPTY------------TSSLGGKIPIRWTAPEAIA-FRKFTSASDAWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAI-EQDYRLPPPPDCP------------------------------TSLHQLMLDCWQKDRNARPRFPQVVSAL----
-L-VLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLK-S--DAT----EKDLSDLI-SEMEMMKMIGK---HKNIINLLGACT--Q------DGPLYVIVEYASKGNLREYLQ----ARRPPGLEY--------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDI---HH--IDYY------------KKTTNGRLPVKWMAPEALF-DRIYTHQSDVWSFGVLLWEIFTLGGSP---------------YPGV-PVEELFKLL-KEGHRMDKPSNCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----
--LTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLK-D--DAT----EKDLSDLV-SEMEMMKMIGK---HKNIINLLGACT--Q------DGPLYVIVEYASKGNLREYLR----ARRPPGMEY--------------------------------------------------SYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDI---NN--IDYY------------KKTTNGRLPVKWMAPEALF-DRVYTHQSDVWSFGVLMWEIFTLGGSP---------------YPGI-PVEELFKLL-KEGHRMDKPANCT------------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL----
--LQLIKRLGNGQFGEVWMGTWN--------GNTKVAIKTLK-P--GTM----SP--ESFL-EEAQIMKKL-K---HDKLVQLYAVVS---------EEPIYIVTEYMNKGSLLDFLK----DG----------------------------------------------------------------EGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLI----E--DNEY------------TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILLTELVTKGRVP---------------YPGM-NNREVLEQV-ERGYRMPCPQDCP------------------------------ISLHELMIHCWKKDPEERPTFEYLQSFL----
--LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-H--STE-----EHLRDFE-REIEILKSL-Q---HDNIVKYKGVCYSAG------RRNLKLIMEYLPYGSLRDYLQ----KH-----------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVL--PQD--KEYY------------KVKEPGESPIFWYAPESLT-ESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM-IVFHLIELL-KNNGRLPRPDGCP------------------------------DEIYMIMTECWNNNVNQRPSFRDLA----LRV
-L-KYISQLGKGNFGSVELCRYDP---LGDNTGALVAVKQLQ-H--SGP----DQ-QRDFQ-REIQILKAL-H---SDFIVKYRGVSYGPG------RQSLRLVMEYLPSGCLRDFLQ----RH-----------------------------------------------------------------RARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVESEAHVKIADFGLAKLL--PLD--KDYY------------VVREPGQSPIFWYAPESLS-DNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAE--FLRMMGCERDVPALCRLLELL-EEGQRLPAPPACP------------------------------AEVHELMKLCWAPSPQDRPSFSALGP---QL-
--LKLGKPLGRGAFGQVIEADAFG--IDKTATCRTVAVKMLK-E--GAT----HSEHRALM-SELKILIHIGH---HLNVVNLLGACT-KP------GGPLMVIVEFCKFGNLSTYLR----SKRNEFVPYKTKGARFRQGKDYVGAIPVDLKRRLDSITSSQSSASSGFVEEKSLSDVEEEEAPEDLYKDFLTLEHLICYSFQVAKGMEFLASRKCIHRDLAARNILLSEKNVVKICDFGLARDI---YK--DPDY------------VRKGDARLPLKWMAPETIF-DRVYTIQSDVWSFGVLLWEIFSLGASP---------------YPGVKIDEEFCRRL-KEGTRMRAPDYTT------------------------------PEMYQTMLDCWHGEPSQRPTFSELVEHL----
--LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q--GSM----SP--DAFL-AEANLMKQL-Q---HQRLVRLYAVVT---------QEPIYIITEYMENGSLVDFLK----TP----------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLI----E--DNEY------------TAREGAKFPIKWTAPEAIN-YGTFTIKSDVWSFGILLTEIVTHGRIP---------------YPGM-TNPEVIQNL-ERGYRMVRPDNCP------------------------------EELYQLMRLCWKERPEDRPTFDYLRSV--L--
LI--HGEVLGKGCFGQAIKVTHR-------ETGEVMVMKELI----RFD----EETQRTFL-KEVKVMRCL-E---HPNVLKFIGVLY--K------DKRLNFITEYIKGGTLRGIIK----SM-----------------------------------------------------------------DSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLM---VD--EKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMIN-GRSYDEKVDVFSFGIVLCEIIGRVNAD---------------PDYLPRTMDFGLNV-RGFLDRYCPPNCP------------------------------PSFFPITVRCCDLDPEKRPSFVKLEH---WL-
--YQNLSPVGSGAYGSVCAAFDT-------KTGLRVAVKKLSRP--FQSI---IH--AKRTYRELRLLKHM-K---HENVIGLLDVFT--PARSLEEFNDVYLVTHLMGA-DLNNIVK----CQ-------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLAR-------HTDDEM------------TG---YVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLT-GRTL---------------FPGT-DHIDQLKLI-LRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYF--
-F-TLCRKLGSGYFGEVFEGLWK--------DRVQVAIKVIS-R--DNL----LH-QQMLQ-SEIQAMKKL-R---HKHILALYAVVS--V------GDPVYIITELMAKGSLLELLR----DS----------------------------------------------------------------DEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLI-------KEDV------------YLSHDHNIPYKWTAPEALS-RGHYSTKSDVWSFGILLHEMFSRGQVP---------------YPGM-SNHEAFLRV-DAGYRMPCPLECP------------------------------PSVHKLMLTCWCRDPEQRPCFKALRERL----
-L-VLGKTLGEGEFGKVVKATAFH--LKGRAGYTTVAVKMLK-E--NAS----PSELRDLL-SEFNVLKQV-N---HPHVIKLYGACS--Q------DGPLLLIVEYAKYGSLRGFLR----ESRKVGPGYLGSG------------------------------------------GSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDV---YE--EDSY------------VKRSQGRIPVKWMAIESLF-DHIYTTQSDVWSFGVLLWEIVTLGGNP---------------YPGI-PPERLFNLL-KTGHRMERPDNCS------------------------------EEMYRLMLQCWKQEPDKRPVFADISKDL----
-L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P--GTM----SP--EAFL-QEAQVMKKL-R---HEKLVQLYAVVS---------EEPIYIVTEYMSKGSLLDFLK----GE----------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLI----E--DNEY------------TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILLTELTTKGRVP---------------YPGM-VNREVLDQV-ERGYRMPCPPECP------------------------------ESLHDLMCQCWRKEPEERPTFEYLQAF--L--
--IKFQDVIGEGNFGQVLKARIK-----KDGLRMDAAIKRMK-E--YAS----KDDHRDFA-GELEVLCKLGH---HPNIINLLGACE--H------RGYLYLAIEYAPHGNLLDFLR----KSRVLETDP--------------------------------------------------AFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQ--------EVY------------VKKTMGRLPVRWMAIESLN-YSVYTTNSDVWSYGVLLWEIVSLGGTP---------------YCGM-TCAELYEKL-PQGYRLEKPLNCD------------------------------DEVYDLMRQCWREKPYERPSFAQILV---SL-
-L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVLSQ----PEAMDDFI-REVNAMHSL-D---HRNLIRLYGVVL---------TPPMKMVTELAPLGSLLDRLR----KH-----------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRAL--PQN--DDHY------------VMQEHRKVPFAWCAPESLK-TRTFSHASDTWMFGVTLWEMFTYGQEP---------------WIGL-NGSQILHKIDKEGERLPRPEDCP------------------------------QDIYNVMVQCWAHKPEDRPTFVALRDF--L--
-L-AFIKEIGSGQFGVVHLGEWR--------SHIQVAIKAIN-E--GSM----SE--EDFI-EEAKVMMKL-S---HSKLVQLYGVCI--Q------RKPLYIVTEFMENGCLLNYLR----EN-----------------------------------------------------------------KGKLRKEMLLSVCQDICEGMEYLERNGYIHRDLAARNCLVSSTCIVKISDFGMTRYV----L--DDEY------------VSSFGAKFPIKWSPPEVFL-FNKYSSKSDVWSFGVLMWEVFTEGKMP---------------FENK-SNLQVVEAI-SEGFRLYRPHLAP------------------------------MSIYEVMYSCWHEKPEGRPTFAELLR---AV-
-L-RLEVKLGQGCFGEVWMGTWN--------GTTKVAIKTLK-P--GTM----MP--EAFL-QEAQIMKKL-R---HDKLVPLYAVVS---------EEPIYIVTEFMSKGSLLDFLK----EG----------------------------------------------------------------DGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLI----E--DNEY------------TARQGAKFPIKWTAPEAAL-YGRFTIKSDVWSFGILQTELVTKGRVP---------------YPGM-VNREVLEQV-ERGYRMPCPQGCP------------------------------ESLHELMNLCWKKDPDERPTFEYIQSFL----
|