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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK ROS1 203
TK ALK 1116
CMGC CDK2 4
TK CSF1R 582
TK DDR1 572
TK EGFR 712
TK FER 563
TK FES 561
TK FGFR1 478
TK FLT4 845
TK INSR 1023
TK JAK1 875
TK JAK2 849
TK JAK3 822
TK KDR 834
TK LTK 510
TKL MAP3K7 36
TK MET 1078
TK MUSK 575
Other NEK2 8
Other NEK5 4
TK NTRK2 538
TK NTRK3 538
CMGC PCTK2 192
TK RET 724
TKL RIPK2 18
TKL RIPK4 22
TKL TNNI3K 463
--LT--LRLLLGSGAFGEVYEGTAVDI----------LGVGSGEIKVAVKTLK-K--GST-DQEKIEFL-K-EAHLMSKF-NHPNILKQLGVCL--L--NEP--QYIILELMEGGDLLTYLR-----KARM--------------------------------------------------------------ATFYG-PLL---TLVDLVDLCVDISKGCVYLER--M-----H---FIHRDLAARNCLVSVKDYTSPR-IV-KIGDFGLARDI---Y---K--NDYY--------------RKRGEGLLPVRWMAPESLM---DGI-FTTQSDVWSFGILIWEILTLGH-QP---------------YPAH---SNLDVLNYV----QTGGR---LEPPRNCP-----------------------------DDLWNLMTQCWAQEPDQRPTFHRIQDQL-------
---I-TLIRGLGHGAFGEVYEGQVSG-----------MPNDPSPLQVAVKTLP-E--VCS-EQDELDFL-M-EALIISKF-NHQNIVRCIGVSL--Q--SLP--RFILLELMAGGDLKSFLR-----ETRP--------------------------------------------------------------RPSQP-SSL---AMLDLLHVARDIACGCQYLEE--N-----H---FIHRDIAARNCLLTCP--GPGR-VA-KIGDFGMARDI---Y---R--ASYY--------------RKGGCAMLPVKWMPPEAFM---EGI-FTSKTDTWSFGVLLWEIFSLGY-MP---------------YPSK---SNQEVLEFV----TSGGR---MDPPKNCP-----------------------------GPVYRIMTQCWQHQPEDRPNFAIILER--I-----
----FQKVEKIGEGTYGVVYKARNK----------------LTGEVVALKKIRLD--TETEGV--PSTAIR-EISLLKEL-NHPNIVKLLDVIH--T--ENK--LYLVFEFLHQ-DLKKFMDA--SALT-----------------------------------------------------------------------GI---PLPLIKSYLFQLLQGLAFCHS--H-----R---VLHRDLKPQNLLINT-----EG-AI-KLADFGLARAF-------GVPVRTY--------------TH---EVVTLWYRAPEILL--GCKY-YSTAVDIWSLGCIFAEMVT-RR-AL---------------FPGD---SEIDQLFRI----FRTLG---TPDEVVWPGVTSMPDYKPSFPKWAR-QDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFF-----
----LQFGKTLGAGAFGKVVEATAFG-----------LGKEDAVLKVAVKMLK-S--TAH-ADEKEALM-S-ELKIMSHLGQHENIVNLLGACT--H--GGP--VLVITEYCCYGDLLNFLR-----RKAEAMLGPSLSPGQDPEGGVDYKNIHLEKKYVRRDSGFSSQGVDTYVEMRPVSTSSNDSFSEQDLDKEDG-RPL---ELRDLLHFSSQVAQGMAFLAS--K-----N---CIHRDVAARNVLLTN-----GH-VA-KIGDFGLARDI---M---N--DSNY--------------IVKGNARLPVKWMAPESIF---DCV-YTVQSDVWSYGILLWEIFSLGL-NP---------------YPGI-L-VNSKFYKLV----KDGYQ---MAQPAFAP-----------------------------KNIYSIMQACWALEPTHRPTFQQICSFL-------
----LRFKEKLGEGQFGEVHLCEVDSPQDLVSLDFPLNVRKGHPLLVAVKILR-P--DAT-KNARNDFL-K-EVKIMSRL-KDPNIIRLLGVCV--Q--DDP--LCMITDYMENGDLNQFLS-----AHQLEDKAAE--------------------------------------------------GAPGDGQAAQG-PTI---SYPMLLHVAAQIASGMRYLAT--L-----N---FVHRDLATRNCLVGE-----NF-TI-KIADFGMSRNL---Y---A--GDYY--------------RVQGRAVLPIRWMAWECIL---MGK-FTTASDVWAFGVTLWEVLMLCRAQP---------------FGQL---TDEQVIENAGEFFRDQGRQVYLSRPPACP-----------------------------QGLYELMLRCWSRESEQRPPFSQLHRFL-------
---F-KKIKVLGSGAFGTVYKGLWIP------------EGEKVKIPVAIKELR-E--ATS-PKANKEIL-D-EAYVMASV-DNPHVCRLLGICL-----TST--VQLITQLMPFGCLLDYVR-----EH--------------------------------------------------------------------K-DNI---GSQYLLNWCVQIAKGMNYLED--R-----R---LVHRDLAARNVLVKT-----PQ-HV-KITDFGLAKLL---G---A--EEKE--------------YHAEGGKVPIKWMALESIL---HRI-YTHQSDVWSYGVTVWELMTFGS-KP---------------YDGI---PASEISSIL----EKGER---LPQPPICT-----------------------------IDVYMIMVKCWMIDADSRPKFRELI----IEF---
--VI--LGELLGKGNFGEVYKGTLK-----------------DKTSVAVKTCK-E--DLP-QELKIKFL-Q-EAKILKQY-DHPNIVKLIGVCT--Q--RQP--VYIIMELVSGGDFLTFLR-----RK--------------------------------------------------------------------K-DEL---KLKQLVKFSLDAAAGMLYLES--K-----N---CIHRDLAARNCLVGE-----NN-VL-KISDFGMSRQE-------D--GGVY--------------SSSGLKQIPIKWTAPEALN---YGR-YSSESDVWSFGILLWETFSLGV-CP---------------YPGM---TNQQAREQV----ERGYR---MSAPQHCP-----------------------------EDISKIMMKCWDYKPENRPKFSELQKEL-------
---L-VLGEQIGRGNFGEVFSGRLR----------------ADNTLVAVKSCR-E--TLP-PDLKAKFL-Q-EARILKQY-SHPNIVRLIGVCT--Q--KQP--IYIVMELVQGGDFLTFLR-----TE--------------------------------------------------------------------G-ARL---RVKTLLQMVGDAAAGMEYLES--K-----C---CIHRDLAARNCLVTE-----KN-VL-KISDFGMSREE---A---D--GVYA--------------ASGGLRQVPVKWTAPEALN---YGR-YSSESDVWSFGILLWETFSLGA-SP---------------YPNL---SNQQTREFV----EKGGR---LPCPELCP-----------------------------DAVFRLMEQCWAYEPGQRPSFSTIYQEL-------
---L-VLGKPLGEGCFGQVVLAEAIGL---------DKDKPNRVTKVAVKMLK-S--DAT-EKDLSDLI-S-EMEMMKMIGKHKNIINLLGACT--Q--DGP--LYVIVEYASKGNLREYLQ-----ARRPPGLEY-----------------------------------------------------CYNPSHNPE-EQL---SSKDLVSCAYQVARGMEYLAS--K-----K---CIHRDLAARNVLVTE-----DN-VM-KIADFGLARDI---H---H--IDYY--------------KKTTNGRLPVKWMAPEALF---DRI-YTHQSDVWSFGVLLWEIFTLGG-SP---------------YPGV---PVEELFKLL----KEGHR---MDKPSNCT-----------------------------NELYMMMRDCWHAVPSQRPTFKQLVEDL-------
----LHLGRVLGYGAFGKVVEASAFG-----------IHKGSSCDTVAVKMLK-E--GAT-ASEHRALM-S-ELKILIHIGNHLNVVNLLGACT-KP--QGP--LMVIVEFCKYGNLSNFLR-----AKRDAFSPCAEKSPEQRGRFRAMVELARLDRRR-----PGSSDRVLFARFSKTEGGARRASPDQEAEDLWL-SPL---TMEDLVCYSFQVARGMEFLAS--R-----K---CIHRDLAARNILLSE-----SD-VV-KICDFGLARDI---Y---K--DPDY--------------VRKGSARLPLKWMAPESIF---DKV-YTTQSDVWSFGVLLWEIFSLGA-SP---------------YPGV-Q-INEEFCQRL----RDGTR---MRAPELAT-----------------------------PAIRRIMLNCWSGDPKARPAFSELVEIL-------
----ITLLRELGQGSFGMVYEGNARD-----------IIKGEAETRVAVKTVN-E--SAS-LRERIEFL-N-EASVMKGF-TCHHVVRLLGVVS--K--GQP--TLVVMELMAHGDLKSYLR-----SLRPEA-----------------------------------------------------------ENNPGR-PPP---TLQEMIQMAAEIADGMAYLNA--K-----K---FVHRDLAARNCMVAH-----DF-TV-KIGDFGMTRDI---Y---E--TDYY--------------RKGGKGLLPVRWMAPESLK---DGV-FTTSSDMWSFGVVLWEITSLAE-QP---------------YQGL---SNEQVLKFV----MDGGY---LDQPDNCP-----------------------------ERVTDLMRMCWQFNPKMRPTFLEIVNLL-------
---L-KRIRDLGEGHFGKVELCRYDP------------EGDNTGEQVAVKSLK-P--ESG-GNHIADLK-K-EIEILRNL-YHENIVKYKGICT-ED---GGNGIKLIMEFLPSGSLKEYLP-----KN--------------------------------------------------------------------K-NKI---NLKQQLKYAVQICKGMDYLGS--R-----Q---YVHRDLAARNVLVES-----EH-QV-KIGDFGLTKAI--ET---D--KEYY--------------TVKDDRDSPVFWYAPECLM---QSK-FYIASDVWSFGVTLHELLTYCD-SDSSPMALF-LKMIGPTHGQM---TVTRLVNTL----KEGKR---LPCPPNCP-----------------------------DEVYQLMRKCWEFQPSNRTSFQNLIE---GF----
----LKFLQQLGKGNFGSVEMCRYDP------------LQDNTGEVVAVKKLQ-H--STE--EHLRDFE-R-EIEILKSL-QHDNIVKYKGVCYSAG--RRN--LKLIMEYLPYGSLRDYLQ-----KH--------------------------------------------------------------------K-ERI---DHIKLLQYTSQICKGMEYLGT--K-----R---YIHRDLATRNILVEN-----EN-RV-KIGDFGLTKVL--PQ---D--KEYY--------------KVKEPGESPIFWYAPESLT---ESK-FSVASDVWSFGVVLYELFTYIE-KSKSPPAEFMRMIGNDKQGQM---IVFHLIELL----KNNGR---LPRPDGCP-----------------------------DEIYMIMTECWNNNVNQRPSFRDLA----LRV---
---L-KYISQLGKGNFGSVELCRYDP------------LGDNTGALVAVKQLQ-H--SGP-DQ-QRDFQ-R-EIQILKAL-HSDFIVKYRGVSYGPG--RQS--LRLVMEYLPSGCLRDFLQ-----RH--------------------------------------------------------------------R-ARL---DASRLLLYSSQICKGMEYLGS--R-----R---CVHRDLAARNILVES-----EA-HV-KIADFGLAKLL--PL---D--KDYY--------------VVREPGQSPIFWYAPESLS---DNI-FSRQSDVWSFGVVLYELFTYCD-KSCSPSAE--FLRMMGCERDV-P-ALCRLLELL----EEGQR---LPAPPACP-----------------------------AEVHELMKLCWAPSPQDRPSFSALGP---QL----
----LKLGKPLGRGAFGQVIEADAFG-----------IDKTATCRTVAVKMLK-E--GAT-HSEHRALM-S-ELKILIHIGHHLNVVNLLGACT-KP--GGP--LMVIVEFCKFGNLSTYLR-----SKRNEFVPYKTKGARFRQGKDYVGAIPVDLKRRL---DSITSSQSSASSGFVEEKSLSDVEEEEAPEDLYK-DFL---TLEHLICYSFQVAKGMEFLAS--R-----K---CIHRDLAARNILLSE-----KN-VV-KICDFGLARDI---Y---K--DPDY--------------VRKGDARLPLKWMAPETIF---DRV-YTIQSDVWSFGVLLWEIFSLGA-SP---------------YPGV-K-IDEEFCRRL----KEGTR---MRAPDYTT-----------------------------PEMYQTMLDCWHGEPSQRPTFSELVEHL-------
---V-TLLRALGHGAFGEVYEGLVIG-----------LPGDSSPLQVAIKTLP-E--LCS-PQDELDFL-M-EALIISKF-RHQNIVRCVGLSL--R--ATP--RLILLELMSGGDMKSFLR-----HSRP--------------------------------------------------------------HLGQP-SPL---VMRDLLQLAQDIAQGCHYLEE--N-----H---FIHRDIAARNCLLSCA--GPSR-VA-KIGDFGMARDI---Y---R--ASYY--------------RRGDRALLPVKWMPPEAFL---EGI-FTSKTDSWSFGVLLWEIFSLGY-MP---------------YPGR---TNQEVLDFV----VGGGR---MDPPRGCP-----------------------------GPVYRIMTQCWQHEPELRPSFASILERL-------
----IEVEEVVGRGAFGVVCKAKWR------------------AKDVAIKQIE------S-ESERKAFI-V-ELRQLSRV-NHPNIVKLYGACL------NP--VCLVMEYAEGGSLYNVLH-----GA-------------------------------------------------------------------EPLPYY---TAAHAMSWCLQCSQGVAYLHS--M-----QPKALIHRDLKPPNLLLVA-----GGTVL-KICDFGTACDI-------------Q--------------THMTNNKGSAAWMAPEVFE---GSN-YSEKCDVFSWGIILWEVITR-R-KP---------------FDEIGG-PAFRIMWAV----HNGTR---PPLIKNLP-----------------------------KPIESLMTRCWSKDPSQRPSMEEIVKI--M-----
----VHFNEVIGRGHFGCVYHGTLL-------------DNDGKKIHCAVKSLN-R--ITD-IGEVSQFL-T-EGIIMKDF-SHPNVLSLLGICL-RS--EGS--PLVVLPYMKHGDLRNFIR-----NE--------------------------------------------------------------------T-HNP---TVKDLIGFGLQVAKGMKYLAS--K-----K---FVHRDLAARNCMLDE-----KF-TV-KVADFGLARDM---Y---D--KEYYS------------VHNKTGAKLPVKWMALESLQ---TQK-FTTKSDVWSFGVLLWELMTRGA-PP---------------YPDV---NTFDITVYL----LQGRR---LLQPEYCP-----------------------------DPLYEVMLKCWHPKAEMRPSFSELVSR--I-----
---I-EYVRDIGEGAFGRVFQARAPG-----------LLPYEPFTMVAVKMLK-E--EAS-ADMQADFQ-R-EAALMAEF-DNPNIVKLLGVCA--V--GKP--MCLLFEYMAYGDLNEFLR-----SMSPHTVCSLSHS---------------------------------------------DLSMRAQVSSPGP-PPL---SCAEQLCIARQVAAGMAYLSE--R-----K---FVHRDLATRNCLVGE-----NM-VV-KIADFGLSRNI---Y---S--ADYY--------------KANENDAIPIRWMPPESIF---YNR-YTTESDVWAYGVVLWEIFSYGL-QP---------------YYGM---AHEEVIYYV----RDGNI---LSCPENCP-----------------------------VELYNLMRLCWSKLPADRPSFTSIHRIL-------
----YEVLYTIGTGSYGRCQKIRRK----------------SDGKILVWKELDYG--SMTEAE--KQMLVS-EVNLLREL-KHPNIVRYYDRII--DRTNTT--LYIVMEYCEGGDLASVITKGTKERQ-----------------------------------------------------------------------YL---DEEFVLRVMTQLTLALKECHR--RSDGGHT---VLHRDLKPANVFLDG-----KQ-NV-KLGDFGLARIL-------NHDTSFA--------------KT---FVGTPYYMSPEQMN---RMS-YNEKSDIWSLGCLLYELCA-LM-PP---------------FTAF------SQKELA----GKIRE---GKFRRIPYRY---S-----------------------DELNEIITRMLNLKDYHRPSVEEILENPL-I----
----YDVIKAIGQGAFGKAYLAKGK----------------SDSKHCVIKEINFE--KMPIQE--KEASKK-EVILLEKM-KHPNIVAFFNSFQ--E--NGR--LFIVMEYCDGGDLMKRINR--QRGV-----------------------------------------------------------------------LF---SEDQILGWFVQISLGLKHIHD--R-----K---ILHRDIKAQNIFLSK-----NG-MVAKLGDFGIARVL-------NNSMELA--------------RT---CIGTPYYLSPEICQ---NKP-YNNKTDIWSLGCVLYELCT-LK-HP---------------FEGN------NLQQLV----LKICQ---AHFAPISPGF---S-----------------------RELHSLISQLFQVSPRDRPSINSILKRPFL-----
---I-VLKRELGEGAFGKVFLAECYN-----------LCPEQDKILVAVKTLK-D--ASD-NA-RKDFH-R-EAELLTNL-QHEHIVKFYGVCV--E--GDP--LIMVFEYMKHGDLNKFLR-----AHGPDAV--------------------------------------------------------LMAEGNPP-TEL---TQSQMLHIAQQIAAGMVYLAS--Q-----H---FVHRDLATRNCLVGE-----NL-LV-KIGDFGMSRDV---Y---S--TDYY--------------RVGGHTMLPIRWMPPESIM---YRK-FTTESDVWSLGVVLWEIFTYGK-QP---------------WYQL---SNNEVIECI----TQGRV---LQRPRTCP-----------------------------QEVYELMLGCWQREPHMRKNIKGIHTL--L-----
---I-VLKRELGEGAFGKVFLAECYN-----------LSPTKDKMLVAVKALK-D--PTL-AA-RKDFQ-R-EAELLTNL-QHEHIVKFYGVCG--D--GDP--LIMVFEYMKHGDLNKFLR-----AHGPDAMIL-----------------------------------------------------VDGQPRQAK-GEL---GLSQMLHIASQIASGMVYLAS--Q-----H---FVHRDLATRNCLVGA-----NL-LV-KIGDFGMSRDV---Y---S--TDYYRLFNPSGNDFCIWCEVGGHTMLPIRWMPPESIM---YRK-FTTESDVWSFGVILWEIFTYGK-QP---------------WFQL---SNTEVIECI----TQGRV---LERPRVCP-----------------------------KEVYDVMLGCWQREPQQRLNIKEIYKIL-------
---Y-IKLEKLGEGTYATVYKGRSK----------------LTENLVALKEIRLE--HEEGAP--C-TAIR-EVSLLKDL-KHANIVTLHDIVH--T--DKS--LTLVFEYLDK-DLKQYMD---DCGN-----------------------------------------------------------------------IM---SMHNVKLFLYQILRGLAYCHR--R-----K---VLHRDLKPQNLLINE-----KG-EL-KLADFGLARAK-------SVPTKTY--------------SN---EVVTLWYRPPDVLL--GSSE-YSTQIDMWGVGCIFFEMAS-GR-PL---------------FPGS---TVEDELHLI----FRLLG---TPSQETWPGISSNEEFKNYNFPKYKPQPLINHAPRLDSEGIELITKFLQYESKKRVSAEEAMKHVYF-----
---L-VLGKTLGEGEFGKVVKATAFH-----------LKGRAGYTTVAVKMLK-E--NAS-PSELRDLL-S-EFNVLKQV-NHPHVIKLYGACS--Q--DGP--LLLIVEYAKYGSLRGFLR-----ESRKVGPGYLGSG---------------------------------------------GSRNSSSLDHPDE-RAL---TMGDLISFAWQISQGMQYLAE--M-----K---LVHRDLAARNILVAE-----GR-KM-KISDFGLSRDV---Y---E--EDSY--------------VKRSQGRIPVKWMAIESLF---DHI-YTTQSDVWSFGVLLWEIVTLGG-NP---------------YPGI---PPERLFNLL----KTGHR---MERPDNCS-----------------------------EEMYRLMLQCWKQEPDKRPVFADISKDL-------
-LAD---LRYLSRGASGTVSSARHA----------------DWRVQVAVKHLHIH--TPLLDS--ERKDVLREAEILHKA-RFSYILPILGICN--E--PEF--LGIVTEYMPNGSLNELLH----RKT-----------------------------------------------------------------------EYPDVAWPLRFRILHEIALGVNYLHN--MT---PP---LLHHDLKTQNILLDN-----EF-HV-KIADFGLSKWRMM--SLSQSRSSKS--------------AP---EGGTIIYMPPENYEPGQKSR-ASIKHDIYSYAVITWEVLS-RK-QP---------------FEDVT-NPLQIMYSVS----QGHRP---VINEESLPYDIPHR-----------------------ARMISLIESGWAQNPDERPSFLKCL-----IEL--
FTGW----EKVGSGGFGQVYKVRHV----------------HWKTWLAIKCSPSL--HVDDRE--RMELLE-EAKKMEMA-KFRYILPVYGIC------REP--VGLVMEYMETGSLEKLLA-----SE-----------------------------------------------------------------------PL---PWDLRFRIIHETAVGMNFLHC--MA---PP---LLHLDLKPANILLDA-----HY-HV-KISDFGLAKCNG----LSHSHDLSM--------------DG---LFGTIAYLPPERIR-EKSRL-FDTKHDVYSFAIVIWGVLT-QK-KP---------------FADE--KNILHIMVKV----VKGHR---PELPPVCRARPRAC-----------------------SHLIRLMQRCWQGDPRVRPTFQ--------GNGLN
----IEFHEIIGSGSFGKVYKGRCR------------------NKIVAIKRYR-ANTYCS-KSDVDMFC-R-EVSILCQL-NHPCVIQFVGACL-ND--PSQ--FAIVTQYISGGSLFSLLH-----EQ--------------------------------------------------------------------K-RIL---DLQSKLIIAVDVAKGMEYLHNLTQ-----P---IIHRDLNSHNILLYE-----DG-HA-VVADFGESRFL---Q---S--LD-E--------------DNMTKQPGNLRWMAPEVFT---QCTRYTIKADVFSYALCLWEILT-GE-IP---------------FAHL---KPAAAAADM---AYHHIR---PPIGYSIP-----------------------------KPISSLLIRGWNACPEGRPEFSEVVMKL-------
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