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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK ROS1 203
TK ABL1 261
TK ALK 1116
TK BLK 241
TK BMX 422
TKL BRAF 456
TK BTK 402
TK EGFR 712
TK EPHB1 619
TK FGFR1 478
TK FGFR2 481
TK FGFR3 472
TK FGFR4 467
TK FYN 271
TK HCK 241
TK INSR 1023
TK JAK2 849
TK KIT 589
TK LCK 245
TK LYN 247
CMGC MAPK14 24
TK NTRK2 538
TK PDGFRA 593
TK PDGFRB 600
TKL RAF1 349
TK RET 724
TK SRC 270
TK SYK 371
TK TNK2 150
TK YES1 277
-LT--LRLLLGSGAFGEVYEGTAVDI-LGVGSGEIKVAVKTLK-K--GST-DQE-KIEFL-KEAHLMSKF-NHPNILKQLGVCL--L------NEPQYIILELMEGGDLLTYLRKARM--------------------------------------------------------------------------------------------ATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDI---YK--NDYY-RKRGEGLLPVRWMAPESLM-DGI---FTTQSDVWSFGILIWEILTLGHQP---------------YPAH-SNLDVLNYV-QTGGR----LEPPRNCP------------------------------DDLWN-LMTQCWAQEPDQRPTFHRIQDQL----
---ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-E--DTM-EV---EEFL-KEAAVMKEI-KHPNLVQLLGVCT--R------EPPFYIITEFMTYGNLLDYLREC-------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGE-----NHLVKVADFGLSRLM----T--GDTY-TAHAGAKFPIKWTAPESLA-YNK---FSIKSDVWAFGVLLWEIATYGMSP---------------YPGI-DLSQVYELL-EKDYR----MERPEGCP------------------------------EKVYE-LMRACWQWNPSDRPSFAEIHQA--F--
--I-TLIRGLGHGAFGEVYEGQVSG--MPNDPSPLQVAVKTLP-E--VCS-EQD-ELDFL-MEALIISKF-NHQNIVRCIGVSL--Q------SLPRFILLELMAGGDLKSFLRETRP--------------------------------------------------------------------------------------------RPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCP--GPGRVAKIGDFGMARDI---YR--ASYY-RKGGCAMLPVKWMPPEAFM-EGI---FTSKTDTWSFGVLLWEIFSLGYMP---------------YPSK-SNQEVLEFV-TSGGR----MDPPKNCP------------------------------GPVYR-IMTQCWQHQPEDRPNFAIILER--I--
--L-RLVRKLGSGQFGEVWMGYYK--------NNMKVAIKTLK-E--GTM-SP---EAFL-GEANVMKAL-QHERLVRLYAVVT---------KEPIYIVTEYMARGCLLDFLKTD-------------------------------------------------------------------------------------------------EGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSE-----ALCCKIADFGLARII-------DSEY-TAQEGAKFPIKWTAPEAIH-FGV---FTIKADVWSFGVLLMEVVTYGRVP---------------YPGM-SNPEVIRNL-ERGYR----MPRPDTCP------------------------------PELYRGVIAECWRSRPEERPTFEFLQSV--L--
---ITLLKELGSGQFGVVQLGKWK--------GQYDVAVKMIK-E--GSM-SE---DEFF-QEAQTMMKL-SHPKLVKFYGVCS--K------EYPIYIVTEYISNGCLLNYLRSH--------------------------------------------------------------------------------------------------GKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDR-----DLCVKVSDFGMTRYV----L--DDQY-VSSVGTKFPVKWSAPEVFH-YFK---YSSKSDVWAFGILMWEVFSLGKQP---------------YDLY-DNSQVVLKV-SQGHR----LYRPHLAS------------------------------DTIYQ-IMYSCWHELPEKRPTFQQLLSS--I--
---ITVGQRIGSGSFGTVYKGKWH----------GDVAVKMLN-V-TAPT-PQQ-LQAFK-NEVGVLRKT-RHVNILLFMGYST---------KPQLAIVTQWCEGSSLYHHLHII--------------------------------------------------------------------------------------------------ETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHE-----DLTVKIGDFGLATVK---SR--WSGS-HQFEQLSGSILWMAPEVIR-MQDKNPYSFQSDVYAFGIVLYELMT-GQLP---------------YSNINNRDQIIFMV-GRGYLSPDLSKVRSNCP------------------------------KAMKR-LMAECLKKKRDERPLFPQILA---SI-
---LTFLKELGTGQFGVVKYGKWR--------GQYDVAIKMIK-E--GSM-SE---DEFI-EEAKVMMNL-SHEKLVQLYGVCT--K------QRPIFIITEYMANGCLLNYLREM--------------------------------------------------------------------------------------------------RHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVND-----QGVVKVSDFGLSRYV----L--DDEY-TSSVGSKFPVRWSPPEVLM-YSK---FSSKSDIWAFGVLMWEIYSLGKMP---------------YERF-TNSETAEHI-AQGLR----LYRPHLAS------------------------------EKVYT-IMYSCWHEKADERPTFKILLSN--I--
--F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E--ATS-PKA-NKEIL-DEAYVMASV-DNPHVCRLLGICL---------TSTVQLITQLMPFGCLLDYVREH--------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKT-----PQHVKITDFGLAKLL---GA--EEKE-YHAEGGKVPIKWMALESIL-HRI---YTHQSDVWSYGVTVWELMTFGSKP---------------YDGI-PASEISSIL-EKGER----LPQPPICT------------------------------IDVYM-IMVKCWMIDADSRPKFRELI----IEF
--V-KIEEVIGAGEFGEVYKGRLK----LPGKREIYVAIKTLK-A--GYS-EKQ-RRDFL-SEASIMGQF-DHPNIIRLEGVVT--K------SRPVMIITEFMENGALDSFLRQN--------------------------------------------------------------------------------------------------DGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNS-----NLVCKVSDFGLSRYLQDDTS--DPTY-TSSLGGKIPVRWTAPEAIA-YRK---FTSASDVWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAI-EQDYR----LPPPMDCP------------------------------AALHQ-LMLDCWQKDRNSRPRFAEIVNTL----
--L-VLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLK-S--DAT-EKD-LSDLI-SEMEMMKMIGKHKNIINLLGACT--Q------DGPLYVIVEYASKGNLREYLQARRPPGLEY-----------------------------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTE-----DNVMKIADFGLARDI---HH--IDYY-KKTTNGRLPVKWMAPEALF-DRI---YTHQSDVWSFGVLLWEIFTLGGSP---------------YPGV-PVEELFKLL-KEGHR----MDKPSNCT------------------------------NELYM-MMRDCWHAVPSQRPTFKQLVEDL----
---LTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLK-D--DAT-EKD-LSDLV-SEMEMMKMIGKHKNIINLLGACT--Q------DGPLYVIVEYASKGNLREYLRARRPPGMEY-----------------------------------------------------------------------------------SYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTE-----NNVMKIADFGLARDI---NN--IDYY-KKTTNGRLPVKWMAPEALF-DRV---YTHQSDVWSFGVLMWEIFTLGGSP---------------YPGI-PVEELFKLL-KEGHR----MDKPANCT------------------------------NELYM-MMRDCWHAVPSQRPTFKQLVEDL----
---LTLGKPLGEGCFGQVVMAEAIGIDKDRAAKPVTVAVKMLK-D--DAT-DKD-LSDLV-SEMEMMKMIGKHKNIINLLGACT--Q------GGPLYVLVEYAAKGNLREFLRARRPPGLDY-----------------------------------------------------------------------------------SFDTCKPPEEQLTFKDLVSCAYQVARGMEYLASQKCIHRDLAARNVLVTE-----DNVMKIADFGLARDV---HN--LDYY-KKTTNGRLPVKWMAPEALF-DRV---YTHQSDVWSFGVLLWEIFTLGGSP---------------YPGI-PVEELFKLL-KEGHR----MDKPANCT------------------------------HDLYM-IMRECWHAAPSQRPTFKQLVEDL----
--L-VLGKPLGEGCFGQVVRAEAFGMDPARPDQASTVAVKMLK-D--NAS-DKD-LADLV-SEMEVMKLIGRHKNIINLLGVCT--Q------EGPLYVIVECAAKGNLREFLRARRPPGPDL-----------------------------------------------------------------------------------SPDGPRSSEGPLSFPVLVSCAYQVARGMQYLESRKCIHRDLAARNVLVTE-----DNVMKIADFGLARGV---HH--IDYY-KKTSNGRLPVKWMAPEALF-DRV---YTHQSDVWSFGILLWEIFTLGGSP---------------YPGI-PVEELFSLL-REGHR----MDRPPHCP------------------------------PELYG-LMRECWHAAPSQRPTFKQLVEAL----
---LQLIKRLGNGQFGEVWMGTWN--------GNTKVAIKTLK-P--GTM-SP---ESFL-EEAQIMKKL-KHDKLVQLYAVVS---------EEPIYIVTEYMNKGSLLDFLKDG-------------------------------------------------------------------------------------------------EGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGN-----GLICKIADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELVTKGRVP---------------YPGM-NNREVLEQV-ERGYR----MPCPQDCP------------------------------ISLHE-LMIHCWKKDPEERPTFEYLQSFL----
---LKLEKKLGAGQFGEVWMATYN--------KHTKVAVKTMK-P--GSM-SV---EAFL-AEANVMKTL-QHDKLVKLHAVVT---------KEPIYIITEFMAKGSLLDFLKSD-------------------------------------------------------------------------------------------------EGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSA-----SLVCKIADFGLARVI----E--DNEY-TAREGAKFPIKWTAPEAIN-FGS---FTIKSDVWSFGILLMEIVTYGRIP---------------YPGM-SNPEVIRAL-ERGYR----MPRPENCP------------------------------EELYN-IMMRCWKNRPEERPTFEYIQSV--L--
---ITLLRELGQGSFGMVYEGNARD--IIKGEAETRVAVKTVN-E--SAS-LRE-RIEFL-NEASVMKGF-TCHHVVRLLGVVS--K------GQPTLVVMELMAHGDLKSYLRSLRPEA-----------------------------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAH-----DFTVKIGDFGMTRDI---YE--TDYY-RKGGKGLLPVRWMAPESLK-DGV---FTTSSDMWSFGVVLWEITSLAEQP---------------YQGL-SNEQVLKFV-MDGGY----LDQPDNCP------------------------------ERVTD-LMRMCWQFNPKMRPTFLEIVNLL----
---LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-H--STE--EH-LRDFE-REIEILKSL-QHDNIVKYKGVCYSAG------RRNLKLIMEYLPYGSLRDYLQKH--------------------------------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVEN-----ENRVKIGDFGLTKVL--PQD--KEYY-KVKEPGESPIFWYAPESLT-ESK---FSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM-IVFHLIELL-KNNGR----LPRPDGCP------------------------------DEIYM-IMTECWNNNVNQRPSFRDLA----LRV
---LSFGKTLGAGAFGKVVEATAYG--LIKSDAAMTVAVKMLK-P--SAH-LTE-REALM-SELKVLSYLGNHMNIVNLLGACT--I------GGPTLVITEYCCYGDLLNFLRRKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM-----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTH-----GRITKICDFGLARDI---KN--DSNY-VVKGNARLPVKWMAPESIF-NCV---YTFESDVWSYGIFLWELFSLGSSP---------------YPGMPVDSKFYKMI-KEGFR----MLSPEHAP------------------------------AEMYD-IMKTCWDADPLKRPTFKQIVQL--I--
---LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q--GSM-SP---DAFL-AEANLMKQL-QHQRLVRLYAVVT---------QEPIYIITEYMENGSLVDFLKTP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSD-----TLSCKIADFGLARLI----E--DNEY-TAREGAKFPIKWTAPEAIN-YGT---FTIKSDVWSFGILLTEIVTHGRIP---------------YPGM-TNPEVIQNL-ERGYR----MVRPDNCP------------------------------EELYQ-LMRLCWKERPEDRPTFDYLRSV--L--
---IKLVKRLGAGQFGEVWMGYYN--------NSTKVAVKTLK-P--GTM-SV---QAFL-EEANLMKTL-QHDKLVRLYAVVT--R------EEPIYIITEYMAKGSLLDFLKSD-------------------------------------------------------------------------------------------------EGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSE-----SLMCKIADFGLARVI----E--DNEY-TAREGAKFPIKWTAPEAIN-FGC---FTIKSDVWSFGILLYEIVTYGKIP---------------YPGR-TNADVMTAL-SQGYR----MPRVENCP------------------------------DELYD-IMKMCWKEKAEERPTFDYLQSV--L--
---YQNLSPVGSGAYGSVCAAFDT-------KTGLRVAVKKLSRP--FQSIIH---AKRTYRELRLLKHM-KHENVIGLLDVFT--PARSLEEFNDVYLVTHLMGA-DLNNIVKCQ----------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNE-----DCELKILDFGLAR-------HTDDEM-TG---YVATRWYRAPEIMLNWMH---YNQTVDIWSVGCIMAELLT-GRTL---------------FPGT-DHIDQLKLI-LRLVG----TPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVD-LLEKMLVLDSDKRITAAQALAHAYF--
--I-VLKRELGEGAFGKVFLAECYN--LCPEQDKILVAVKTLK-D--ASD-NA--RKDFH-REAELLTNL-QHEHIVKFYGVCV--E------GDPLIMVFEYMKHGDLNKFLRAHGPDAV--------------------------------------------------------------------------------------LMAEGNPPTELTQSQMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGE-----NLLVKIGDFGMSRDV---YS--TDYY-RVGGHTMLPIRWMPPESIM-YRK---FTTESDVWSLGVVLWEIFTYGKQP---------------WYQL-SNNEVIECI-TQGRV----LQRPRTCP------------------------------QEVYE-LMLGCWQREPHMRKNIKGIHTL--L--
--L-VLGRVLGSGAFGKVVEGTAYG--LSRSQPVMKVAVKMLK-P--TAR-SSE-KQALM-SELKIMTHLGPHLNIVNLLGACT--K------SGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQA-DTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQ-----GKIVKICDFGLARDI---MH--DSNY-VSKGSTFLPVKWMAPESIF-DNL---YTTLSDVWSYGILLWEIFSLGGTP---------------YPGMMVDSTFYNKI-KSGYR----MAKPDHAT------------------------------SEVYE-IMVKCWNSEPEKRPSFYHLSE---IV-
--L-VLGRTLGSGAFGQVVEATAHG--LSHSQATMKVAVKMLK-S--TAR-SSE-KQALM-SELKIMSHLGPHLNVVNLLGACT--K------GGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICE-----GKLVKICDFGLARDI---MR--DSNY-ISKGSTFLPLKWMAPESIF-NSL---YTTLSDVWSFGILLWEIFTLGGTP---------------YPELPMNEQFYNAI-KRGYR----MAQPAHAS------------------------------DEIYE-IMQKCWEEKFEIRPPFSQLVL---LL-
-VM--LSTRIGSGSFGTVYKGKWH----------GDVAVKILK-V-VDPT-PEQ-FQAFR-NEVAVLRKT-RHVNILLFMGYMT---------KDNLAIVTQWCEGSSLYKHLHVQ--------------------------------------------------------------------------------------------------ETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE-----GLTVKIGDFGLATVK---SR--WSGS-QQVEQPTGSVLWMAPEVIR-MQDNNPFSFQSDVYSYGIVLYELMT-GELP---------------YSHINNRDQIIFMV-GRGYASPDLSKLYKNCP------------------------------KAMKR-LVADCVKKVKEERPLFPQILSS--I--
--L-VLGKTLGEGEFGKVVKATAFH--LKGRAGYTTVAVKMLK-E--NAS-PSE-LRDLL-SEFNVLKQV-NHPHVIKLYGACS--Q------DGPLLLIVEYAKYGSLRGFLRESRKVGPGYLGSG---------------------------------------------------------------------------GSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEMKLVHRDLAARNILVAE-----GRKMKISDFGLSRDV---YE--EDSY-VKRSQGRIPVKWMAIESLF-DHI---YTTQSDVWSFGVLLWEIVTLGGNP---------------YPGI-PPERLFNLL-KTGHR----MERPDNCS------------------------------EEMYR-LMLQCWKQEPDKRPVFADISKDL----
--L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P--GTM-SP---EAFL-QEAQVMKKL-RHEKLVQLYAVVS---------EEPIYIVTEYMSKGSLLDFLKGE-------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGE-----NLVCKVADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELTTKGRVP---------------YPGM-VNREVLDQV-ERGYR----MPCPPECP------------------------------ESLHD-LMCQCWRKEPEERPTFEYLQAF--L--
TLE---DKELGSGNFGTVKKGYYQ-----MKKVVKTVAVKILK-N--EAN-DPALKDELL-AEANVMQQL-DNPYIVRMIGICE---------AESWMLVMEMAELGPLNKYLQQN---------------------------------------------------------------------------------------------------RHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVT-----QHYAKISDFGLSKAL---RA--DENYYKAQTHGKWPVKWYAPECIN-YYK---FSSKSDVWSFGVLMWEAFSYGQKP---------------YRGM-KGSEVTAML-EKGER----MGCPAGCP------------------------------REMYD-LMNLCWTYDVENRPGFAAVE----LRL
--L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVLSQ-PEA-MDDFI-REVNAMHSL-DHRNLIRLYGVVL---------TPPMKMVTELAPLGSLLDRLRKH--------------------------------------------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLAT-----RDLVKIGDFGLMRAL--PQN--DDHY-VMQEHRKVPFAWCAPESLK-TRT---FSHASDTWMFGVTLWEMFTYGQEP---------------WIGL-NGSQILHKIDKEGER----LPRPEDCP------------------------------QDIYN-VMVQCWAHKPEDRPTFVALRDF--L--
--L-RLEVKLGQGCFGEVWMGTWN--------GTTKVAIKTLK-P--GTM-MP---EAFL-QEAQIMKKL-RHDKLVPLYAVVS---------EEPIYIVTEFMSKGSLLDFLKEG-------------------------------------------------------------------------------------------------DGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGE-----NLVCKIADFGLARLI----E--DNEY-TARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILQTELVTKGRVP---------------YPGM-VNREVLEQV-ERGYR----MPCPQGCP------------------------------ESLHE-LMNLCWKKDPDERPTFEYIQSFL----
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