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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK SRC 203
TK ABL1 261
TKL ACVR1 208
TK ALK 1116
TK BLK 241
TKL BRAF 456
CMGC CDK2 4
CAMK CHEK1 9
TK CSK 195
TK EGFR 712
TK EPHB4 615
TK ERBB2 720
TK FGFR1 478
TK FYN 271
TK IGF1R 999
TK INSR 1023
TK JAK1 875
TK JAK2 849
TK JAK3 822
TK KIT 589
TK LCK 245
TK LYN 247
TK PDGFRB 600
AGC PRKCD 349
AGC PRKCQ 380
TKL RAF1 349
AGC RPS6KA1 62
TKL TGFBR1 205
TK TYK2 589
TK YES1 277
-L-RLEVKLGQGCFGEV---WMGTWN--------GTTRVAIKTLK-P-GTM-SP--EAFL-Q-EAQVMKKL--RHEKLVQLYAVVS-------EEP--IYIVTEYMSKGSLLDFLK---GE-------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERM--------NYVHRDLRAANILVGE---NLVCKVADFGLARLI-------E--DNEYTARQGAKFPIKWTAPEAAL------YGR---FTIKSDVWSFGILLTELTTKGR----------VP---------------YPGM-----VNREVLDQVERGYR----MPCPPECP----------------------------ESLHD-LMCQCWRKEPEERPT-----FE-YLQAF--L--
--ITMKHKLGGGQYGEV---YEGVWK-------KYSLTVAVKTLK-E-DTM-EV--EEFL-K-EAAVMKEI--KHPNLVQLLGVCT--R----EPP--FYIITEFMTYGNLLDYLR---EC-------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKK--------NFIHRDLAARNCLVGE---NHLVKVADFGLSRLM-------T--GDTYTAHAGAKFPIKWTAPESLA------YNK---FSIKSDVWAFGVLLWEIATYGM----------SP---------------YPGI-----DLSQVYELLEKDYR----MERPEGCP----------------------------EKVYE-LMRACWQWNPSDRPS-----FA-EIHQA--F--
-I-TLLECVGKGRYGEV---WRGSWQ-------GEN--VAVKIFSSR-DEKSWF--RET----ELYNTVML--RHENILGFIASDM--TSRHSSTQ--LWLITHYHEMGSLYDYLQ---LT----------------------------------------------------------------------------------------------------TLDTVSCLRIVLSIASGLAHLHIEIFGTQGKPAIAHRDLKSKNILVKK---NGQCCIADLGLAVMHSQ---STNQLDVGNNP---RVGTKRYMAPEVLDETIQVDCFD---SYKRVDIWAFGLVLWEVAR-RMVSNGIVEDYKPP---------------FYDVVPNDPSFEDMRKVVCVDQQ----RPNIPNRWFSDPTL----------------------TSLAK-LMKECWYQNPSARLT-----AL-RIKK----TL
-I-TLIRGLGHGAFGEV---YEGQVSG--MPNDPSPLQVAVKTLP-E-VCS-EQDELDFL-M-EALIISKF--NHQNIVRCIGVSL--Q----SLP--RFILLELMAGGDLKSFLR---ETRP--------------------------------------------------------------------------------------------RPSQPSSLAMLDLLHVARDIACGCQYLEEN--------HFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDI----Y--R--ASYYRKGGCAMLPVKWMPPEAFM------EGI---FTSKTDTWSFGVLLWEIFSLGY----------MP---------------YPSK-----SNQEVLEFVTSGGR----MDPPKNCP----------------------------GPVYR-IMTQCWQHQPEDRPN-----FA-IILER--I--
-L-RLVRKLGSGQFGEV---WMGYYK--------NNMKVAIKTLK-E-GTM-SP--EAFL-G-EANVMKAL--QHERLVRLYAVVT-------KEP--IYIVTEYMARGCLLDFLK---TD-------------------------------------------------------------------------------------------------EGSRLSLPRLIDMSAQIAEGMAYIERM--------NSIHRDLRAANILVSE---ALCCKIADFGLARII----------DSEYTAQEGAKFPIKWTAPEAIH------FGV---FTIKADVWSFGVLLMEVVTYGR----------VP---------------YPGM-----SNPEVIRNLERGYR----MPRPDTCP----------------------------PELYRGVIAECWRSRPEERPT-----FE-FLQSV--L--
--ITVGQRIGSGSFGTV---YKGKWH----------GDVAVKMLN-VTAPT-PQQLQAFK-N-EVGVLRKT--RHVNILLFMGYST-------KPQ--LAIVTQWCEGSSLYHHLH---II--------------------------------------------------------------------------------------------------ETKFEMIKLIDIARQTAQGMDYLHAK--------SIIHRDLKSNNIFLHE---DLTVKIGDFGLATVK----S--R--WSGSHQFEQLSGSILWMAPEVIR------MQDKNPYSFQSDVYAFGIVLYELMT-GQ----------LP---------------YSNI-N---NRDQIIFMVGRGYLSPDLSKVRSNCP----------------------------KAMKR-LMAECLKKKRDERPL-----FP-QILA---SI-
--FQKVEKIGEGTYGVV---YKARNK-------LTGEVVALKKIRLD-TETEGV--PSTAIR-EISLLKEL--NHPNIVKLLDVIH--T----ENK--LYLVFEFLHQ-DLKKFMDASALT----------------------------------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHSH--------RVLHRDLKPQNLLINT---EGAIKLADFGLARAF-------GVPVRTYTH---EVVTLWYRAPEILL-----GCKY---YSTAVDIWSLGCIFAEMVT-RR----------AL---------------FPGD-----SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRS-LLSQMLHYDPNKRIS-----AK-AALAHPFF--
-W-DLVQTLGEGAYGEV---QLAVNR-------VTEEAVAVKIVDMK-RAVDCP--ENI-KK-EICINKML--NHENVVKFYGHRR--E----GNI--QYLFLEYCSGGELFDRIE--PDI----------------------------------------------------------------------------------------------------GMPEPDAQRFFHQLMAGVVYLHGI--------GITHRDIKPENLLLDE---RDNLKISDFGLATVFR-----YNNRERLLNK---MCGTLPYVAPELLK-----RREF---HAEPVDVWSCGIVLTAMLA-GE----------LP---------------WDQP-------SDSCQEYSDWKE----KKTYLNPWKKI--D----------------------SAPLA-LLHKILVENPSARIT-----IP-DIKKDRW-Y-
--LKLLQTIGKGEFGDV---MLGDYR---------GNKVAVKCIK-N-DAT-AQ---AFL-A-EASVMTQL--RHSNLVQLLGVIV-EE----KGG--LYIVTEYMAKGSLVDYLR---SR-------------------------------------------------------------------------------------------------GRSVLGGDCLLKFSLDVCEAMEYLEGN--------NFVHRDLAARNVLVSE---DNVAKVSDFGLTKEA-------------SSTQDTGKLPVKWTAPEALR------EKK---FSTKSDVWSFGILLWEIYSFGR----------VP---------------YPRI-----PLKDVVPRVEKGYK----MDAPDGCP----------------------------PAVYE-VMKNCWHLDAAMRPS-----FL-QLREQL----
-F-KKIKVLGSGAFGTV---YKGLWIP---EGEKVKIPVAIKELR-E-ATS-PKANKEIL-D-EAYVMASV--DNPHVCRLLGICL-------TST--VQLITQLMPFGCLLDYVR---EH--------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDR--------RLVHRDLAARNVLVKT---PQHVKITDFGLAKLL----G--A--EEKEYHAEGGKVPIKWMALESIL------HRI---YTHQSDVWSYGVTVWELMTFGS----------KP---------------YDGI-----PASEISSILEKGER----LPQPPICT----------------------------IDVYM-IMVKCWMIDADSRPK-----FR-ELI----IEF
-V-KIEEVIGAGEFGEV---CRGRLK----APGKKESCVAIKTLK-G-GYT-ERQRREFL-S-EASIMGQF--EHPNIIRLEGVVT--N----SMP--VMILTEFMENGALDSFLR---LN--------------------------------------------------------------------------------------------------DGQFTVIQLVGMLRGIASGMRYLAEM--------SYVHRDLAARNILVNS---NLVCKVSDFGLSRFLE-ENS--S--DPTYTSSLGGKIPIRWTAPEAIA------FRK---FTSASDAWSYGIVMWEVMSFGE----------RP---------------YWDM-----SNQDVINAIEQDYR----LPPPPDCP----------------------------TSLHQ-LMLDCWQKDRNARPR-----FP-QVVSAL----
-L-RKVKVLGSGAFGTV---YKGIWIP---DGENVKIPVAIKVLR-E-NTS-PKANKEIL-D-EAYVMAGV--GSPYVSRLLGICL-------TST--VQLVTQLMPYGCLLDHVR---EN--------------------------------------------------------------------------------------------------RGRLGSQDLLNWCMQIAKGMSYLEDV--------RLVHRDLAARNVLVKS---PNHVKITDFGLARLL----D--I--DETEYHADGGKVPIKWMALESIL------RRR---FTHQSDVWSYGVTVWELMTFGA----------KP---------------YDGI-----PAREIPDLLEKGER----LPQPPICT----------------------------IDVYM-IMVKCWMIDSECRPR-----FR-ELVSE--F--
-L-VLGKPLGEGCFGQV---VLAEAIGLDKDKPNRVTKVAVKMLK-S-DAT-EKDLSDLI-S-EMEMMKMIG-KHKNIINLLGACT--Q----DGP--LYVIVEYASKGNLREYLQ---ARRPPGLEY-----------------------------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASK--------KCIHRDLAARNVLVTE---DNVMKIADFGLARDI----H--H--IDYYKKTTNGRLPVKWMAPEALF------DRI---YTHQSDVWSFGVLLWEIFTLGG----------SP---------------YPGV-----PVEELFKLLKEGHR----MDKPSNCT----------------------------NELYM-MMRDCWHAVPSQRPT-----FK-QLVEDL----
--LQLIKRLGNGQFGEV---WMGTWN--------GNTKVAIKTLK-P-GTM-SP--ESFL-E-EAQIMKKL--KHDKLVQLYAVVS-------EEP--IYIVTEYMNKGSLLDFLK---DG-------------------------------------------------------------------------------------------------EGRALKLPNLVDMAAQVAAGMAYIERM--------NYIHRDLRSANILVGN---GLICKIADFGLARLI-------E--DNEYTARQGAKFPIKWTAPEAAL------YGR---FTIKSDVWSFGILLTELVTKGR----------VP---------------YPGM-----NNREVLEQVERGYR----MPCPQDCP----------------------------ISLHE-LMIHCWKKDPEERPT-----FE-YLQSFL----
--ITMSRELGQGSFGMV---YEGVAKG--VVKDEPETRVAIKTVN-E-AAS-MRERIEFL-N-EASVMKEF--NCHHVVRLLGVVS--Q----GQP--TLVIMELMTRGDLKSYLR---SLRPEM-----------------------------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNAN--------KFVHRDLAARNCMVAE---DFTVKIGDFGMTRDI----Y--E--TDYYRKGGKGLLPVRWMSPESLK------DGV---FTTYSDVWSFGVVLWEIATLAE----------QP---------------YQGL-----SNEQVLRFVMEGGL----LDKPDNCP----------------------------DMLFE-LMRMCWQYNPKMRPS-----FL-EIISS--I--
--ITLLRELGQGSFGMV---YEGNARD--IIKGEAETRVAVKTVN-E-SAS-LRERIEFL-N-EASVMKGF--TCHHVVRLLGVVS--K----GQP--TLVVMELMAHGDLKSYLR---SLRPEA-----------------------------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNAK--------KFVHRDLAARNCMVAH---DFTVKIGDFGMTRDI----Y--E--TDYYRKGGKGLLPVRWMAPESLK------DGV---FTTSSDMWSFGVVLWEITSLAE----------QP---------------YQGL-----SNEQVLKFVMDGGY----LDQPDNCP----------------------------ERVTD-LMRMCWQFNPKMRPT-----FL-EIVNLL----
-L-KRIRDLGEGHFGKV---ELCRYDP---EGDNTGEQVAVKSLK-P-ESG-GNHIADLK-K-EIEILRNL--YHENIVKYKGICT-ED-----GGNGIKLIMEFLPSGSLKEYLP---KN--------------------------------------------------------------------------------------------------KNKINLKQQLKYAVQICKGMDYLGSR--------QYVHRDLAARNVLVES---EHQVKIGDFGLTKAI---ET--D--KEYYTVKDDRDSPVFWYAPECLM------QSK---FYIASDVWSFGVTLHELLTYCD----------SDSSPMALF-LKMIGPTHGQM-----TVTRLVNTLKEGKR----LPCPPNCP----------------------------DEVYQ-LMRKCWEFQPSNRTS-----FQ-NLIE---GF-
--LKFLQQLGKGNFGSV---EMCRYDP---LQDNTGEVVAVKKLQ-H-STE--EHLRDFE-R-EIEILKSL--QHDNIVKYKGVCYSAG----RRN--LKLIMEYLPYGSLRDYLQ---KH--------------------------------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTK--------RYIHRDLATRNILVEN---ENRVKIGDFGLTKVL---PQ--D--KEYYKVKEPGESPIFWYAPESLT------ESK---FSVASDVWSFGVVLYELFTYIE----------KSKSPPAEFMRMIGNDKQGQM-----IVFHLIELLKNNGR----LPRPDGCP----------------------------DEIYM-IMTECWNNNVNQRPS-----FR-DLA----LRV
-L-KYISQLGKGNFGSV---ELCRYDP---LGDNTGALVAVKQLQ-H-SGP-DQ-QRDFQ-R-EIQILKAL--HSDFIVKYRGVSYGPG----RQS--LRLVMEYLPSGCLRDFLQ---RH--------------------------------------------------------------------------------------------------RARLDASRLLLYSSQICKGMEYLGSR--------RCVHRDLAARNILVES---EAHVKIADFGLAKLL---PL--D--KDYYVVREPGQSPIFWYAPESLS------DNI---FSRQSDVWSFGVVLYELFTYCD----------KSCSPSAE--FLRMMGCERDV-P---ALCRLLELLEEGQR----LPAPPACP----------------------------AEVHE-LMKLCWAPSPQDRPS-----FS-ALGP---QL-
--LSFGKTLGAGAFGKV---VEATAYG--LIKSDAAMTVAVKMLK-P-SAH-LTEREALM-S-ELKVLSYLG-NHMNIVNLLGACT--I----GGP--TLVITEYCCYGDLLNFLR---RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM-----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASK--------NCIHRDLAARNILLTH---GRITKICDFGLARDI----K--N--DSNYVVKGNARLPVKWMAPESIF------NCV---YTFESDVWSYGIFLWELFSLGS----------SP---------------YPGM-P---VDSKFYKMIKEGFR----MLSPEHAP----------------------------AEMYD-IMKTCWDADPLKRPT-----FK-QIVQL--I--
--LKLVERLGAGQFGEV---WMGYYN--------GHTKVAVKSLK-Q-GSM-SP--DAFL-A-EANLMKQL--QHQRLVRLYAVVT-------QEP--IYIITEYMENGSLVDFLK---TP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEER--------NYIHRDLRAANILVSD---TLSCKIADFGLARLI-------E--DNEYTAREGAKFPIKWTAPEAIN------YGT---FTIKSDVWSFGILLTEIVTHGR----------IP---------------YPGM-----TNPEVIQNLERGYR----MVRPDNCP----------------------------EELYQ-LMRLCWKERPEDRPT-----FD-YLRSV--L--
--IKLVKRLGAGQFGEV---WMGYYN--------NSTKVAVKTLK-P-GTM-SV--QAFL-E-EANLMKTL--QHDKLVRLYAVVT--R----EEP--IYIITEYMAKGSLLDFLK---SD-------------------------------------------------------------------------------------------------EGGKVLLPKLIDFSAQIAEGMAYIERK--------NYIHRDLRAANVLVSE---SLMCKIADFGLARVI-------E--DNEYTAREGAKFPIKWTAPEAIN------FGC---FTIKSDVWSFGILLYEIVTYGK----------IP---------------YPGR-----TNADVMTALSQGYR----MPRVENCP----------------------------DELYD-IMKMCWKEKAEERPT-----FD-YLQSV--L--
-L-VLGRTLGSGAFGQV---VEATAHG--LSHSQATMKVAVKMLK-S-TAR-SSEKQALM-S-ELKIMSHLG-PHLNVVNLLGACT--K----GGP--IYIITEYCRYGDLVDYLH---RNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASK--------NCVHRDLAARNVLICE---GKLVKICDFGLARDI----M--R--DSNYISKGSTFLPLKWMAPESIF------NSL---YTTLSDVWSFGILLWEIFTLGG----------TP---------------YPEL-P---MNEQFYNAIKRGYR----MAQPAHAS----------------------------DEIYE-IMQKCWEEKFEIRPP-----FS-QLVL---LL-
FI--FHKVLGKGSFGKV---LLGELK-------GRGEYFAIKALKKD-VVLIDD--DVECTMVEKRVLTLA-AENPFLTHLICTFQ--T----KDH--LFFVMEFLNGGDLMYHIQ--DKG----------------------------------------------------------------------------------------------------RFELYRATFYAAEIMCGLQFLHSK--------GIIYRDLKLDNVLLDR---DGHIKIADFGMCKEN-------IFGESRAST---FCGTPDYIAPEILQ------GLK---YTFSVDWWSFGVLLYEMLI-GQ----------SP---------------FHGD--------DEDELFESIRV----DTPHYPRWIT--------------------------KESKD-ILEKLFEREPTKRLG----VTG-NIKIHPFF--
FI--LHKMLGKGSFGKV---FLAEFK-------KTNQFFAIKALKKD-VVLMDD--DVECTMVEKRVLSLA-WEHPFLTHMFCTFQ--T----KEN--LFFVMEYLNGGDLMYHIQ--SCH----------------------------------------------------------------------------------------------------KFDLSRATFYAAEIILGLQFLHSK--------GIVYRDLKLDNILLDK---DGHIKIADFGMCKEN-------MLGDAKTNT---FCGTPDYIAPEILL------GQK---YNHSVDWWSFGVLLYEMLI-GQ----------SP---------------FHGQ--------DEEELFHSIRM----DNPFYPRWLE--------------------------KEAKD-LLVKLFVREPEKRLG-----VRGDIRQHPLF--
VM--LSTRIGSGSFGTV---YKGKWH----------GDVAVKILK-VVDPT-PEQFQAFR-N-EVAVLRKT--RHVNILLFMGYMT-------KDN--LAIVTQWCEGSSLYKHLH---VQ--------------------------------------------------------------------------------------------------ETKFQMFQLIDIARQTAQGMDYLHAK--------NIIHRDMKSNNIFLHE---GLTVKIGDFGLATVK----S--R--WSGSQQVEQPTGSVLWMAPEVIR------MQDNNPFSFQSDVYSYGIVLYELMT-GE----------LP---------------YSHI-N---NRDQIIFMVGRGYASPDLSKLYKNCP----------------------------KAMKR-LVADCVKKVKEERPL-----FP-QILSS--I--
--FELLKVLGQGSFGKVFLVRKVTRP-------DSGHLYAMKVLKKA-TLKVRD--RVRTKM-ERDILADV--NHPFVVKLHYAFQ--T----EGK--LYLILDFLRGGDLFTRLS--KEV----------------------------------------------------------------------------------------------------MFTEEDVKFYLAELALGLDHLHSL--------GIIYRDLKPENILLDE---EGHIKLTDFGLSKEA-------IDHEKKAYS---FCGTVEYMAPEVVN------RQG---HSHSADWWSYGVLMFEMLT-GS----------LP---------------FQGK--------DRKETMTLILK----AKLGMPQFLS--------------------------TEAQS-LLRALFKRNPANRLGSGPDGAE-EIKRHV--FY
IV--LQESIGKGRFGEV---WRGKWR-------GEE--VAVKIFSSR-EERSWF--REA----EIYQTVML--RHENILGFIAADN--KDNGTWTQ--LWLVSDYHEHGSLFDYLN---RY----------------------------------------------------------------------------------------------------TVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKK---NGTCCIADLGLAVRHDS---ATDTIDIAPNH---RVGTKRYMAPEVLDDSINMKHFE---SFKRADIYAMGLVFWEIAR-RCSIGGIHEDYQLP---------------YYDLVPSDPSVEEMRKVVCEQKL----RPNIPNRWQSCEAL----------------------RVMAK-IMRECWYANGAARLT-----AL-RIKK----TL
-L-KKIRDLGEGHFGKV---SLYCYDP---TNDGTGEMVAVKALK-A-DCG-PQHRSGWK-Q-EIDILRTL--YHEHIIKYKGCCEDQG----EKS--LQLVMEYVPLGSLRDYLP---RH----------------------------------------------------------------------------------------------------SIGLAQLLLFAQQICEGMAYLHAQ--------HYIHRDLAARNVLLDN---DRLVKIGDFGLAKAV---PE--G--HEYYRVREDGDSPVFWYAPECLK------EYK---FYYASDVWSFGVTLYELLTHCD----------SSQSPPTKF-LELIGIAQGQM-----TVLRLTELLERGER----LPRPDKCP----------------------------CEVYH-LMKNCWETEASFRPT-----FE-NLIP---IL-
-L-RLEVKLGQGCFGEV---WMGTWN--------GTTKVAIKTLK-P-GTM-MP--EAFL-Q-EAQIMKKL--RHDKLVPLYAVVS-------EEP--IYIVTEFMSKGSLLDFLK---EG-------------------------------------------------------------------------------------------------DGKYLKLPQLVDMAAQIADGMAYIERM--------NYIHRDLRAANILVGE---NLVCKIADFGLARLI-------E--DNEYTARQGAKFPIKWTAPEAAL------YGR---FTIKSDVWSFGILQTELVTKGR----------VP---------------YPGM-----VNREVLEQVERGYR----MPCPQGCP----------------------------ESLHE-LMNLCWKKDPDERPT-----FE-YIQSFL----
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