|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
TK TNK2 203
TK ABL1 261
TK ALK 1116
Other AURKA 133
TK BTK 402
TK CSF1R 582
TK CSK 195
TK EGFR 712
TK EPHB4 615
TK ERBB2 720
TK FGFR1 478
TK HCK 241
TK INSR 1023
TK ITK 363
TK JAK2 849
TK JAK3 822
TK KIT 589
TK LCK 245
TK PDGFRB 600
TK SRC 270
CAMK STK33 116
TK TEK 824
L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVL-SQ-PEAMDDFI-REVNAMHSL-DHRNLIRLYGVVL---TPPMKMVTELAPLGSLLDRLR-KH--------------------------------------------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLAT---RDLV---------KIGDFGLMRAL--PQ-N--DDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEP---------------WIGL-NGSQILHKIDKEGERLPRPEDCP------QDIYNVMVQCWAHKPEDRPTFVALRDF--L--
-ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-E--D-TM-EV--EEFL-KEAAVMKEI-KHPNLVQLLGVCT--REPPFYIITEFMTYGNLLDYLR-EC-------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGE---NHLV---------KVADFGLSRLM-----T--GDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSP---------------YPGI-DLSQVYELL-EKDYRMERPEGCP------EKVYELMRACWQWNPSDRPSFAEIHQA--F--
I-TLIRGLGHGAFGEVYEGQVSG--MPNDPSPLQVAVKTLP-E--V-CS-EQDELDFL-MEALIISKF-NHQNIVRCIGVSL--QSLPRFILLELMAGGDLKSFLR-ETRP--------------------------------------------------------------------------------------------RPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVA---------KIGDFGMARDI---Y-R--ASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMP---------------YPSK-SNQEVLEFV-TSGGRMDPPKNCP------GPVYRIMTQCWQHQPEDRPNFAIILER--I--
-FEIGRPLGKGKFGNVYLAREK-------QSKFILALKVLFKA--QLEKAGV--EHQLRREVEIQSHL-RHPNILRLYGYFH--DATRVYLILEYAPLGTVYRELQKLS----------------------------------------------------------------------------------------------------KFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGS---AGEL---------KIADFGWSV-------HAPSSRRTT---LCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLV-GKPP---------------FEAN----TYQETY-KRISRVEFTFPDFVT----EGARDLISRLLKHNPSQRPMLREVLEHPWI--
-LTFLKELGTGQFGVVKYGKWR--------GQYDVAIKMIK-E--G-SM-SE--DEFI-EEAKVMMNL-SHEKLVQLYGVCT--KQRPIFIITEYMANGCLLNYLR-EM--------------------------------------------------------------------------------------------------RHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVND---QGVV---------KVSDFGLSRYV-----L--DDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMP---------------YERF-TNSETAEHI-AQGLRLYRPHLAS------EKVYTIMYSCWHEKADERPTFKILLSN--I--
-LQFGKTLGAGAFGKVVEATAFG--LGKEDAVLKVAVKMLK-S--T-AH-ADEKEALM-SELKIMSHLGQHENIVNLLGACT--HGGPVLVITEYCCYGDLLNFLR-RKAEAMLGPSLSPGQDPEGGVDYKNIHLEKKYVRR------------------------------DSGFSSQGVDTYVEMRPVSTSSNDSFSEQDLDKEDGRPLELRDLLHFSSQVAQGMAFLASKNCIHRDVAARNVLLTN---GHVA---------KIGDFGLARDI---M-N--DSNYIVKGNARLPVKWMAPESIFDCVYTVQSDVWSYGILLWEIFSLGLNP---------------YPGILVNSKFYKLV-KDGYQMAQPAFAP------KNIYSIMQACWALEPTHRPTFQQICSFL----
-LKLLQTIGKGEFGDVMLGDYR---------GNKVAVKCIK-N--D-AT-AQ---AFL-AEASVMTQL-RHSNLVQLLGVIV-EEKGGLYIVTEYMAKGSLVDYLR-SR-------------------------------------------------------------------------------------------------GRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSE---DNVA---------KVSDFGLTKEA-----------SSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVP---------------YPRI-PLKDVVPRV-EKGYKMDAPDGCP------PAVYEVMKNCWHLDAAMRPSFLQLREQL----
F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E--A-TS-PKANKEIL-DEAYVMASV-DNPHVCRLLGICL---TSTVQLITQLMPFGCLLDYVR-EH--------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKT---PQHV---------KITDFGLAKLL---G-A--EEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKP---------------YDGI-PASEISSIL-EKGERLPQPPICT------IDVYMIMVKCWMIDADSRPKFRELI----IEF
V-KIEEVIGAGEFGEVCRGRLK----APGKKESCVAIKTLK-G--G-YT-ERQRREFL-SEASIMGQF-EHPNIIRLEGVVT--NSMPVMILTEFMENGALDSFLR-LN--------------------------------------------------------------------------------------------------DGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNS---NLVC---------KVSDFGLSRFLEENS-S--DPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAI-EQDYRLPPPPDCP------TSLHQLMLDCWQKDRNARPRFPQVVSAL----
L-RKVKVLGSGAFGTVYKGIWIP---DGENVKIPVAIKVLR-E--N-TS-PKANKEIL-DEAYVMAGV-GSPYVSRLLGICL---TSTVQLVTQLMPYGCLLDHVR-EN--------------------------------------------------------------------------------------------------RGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKS---PNHV---------KITDFGLARLL---D-I--DETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKP---------------YDGI-PAREIPDLL-EKGERLPQPPICT------IDVYMIMVKCWMIDSECRPRFRELVSE--F--
L-VLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLK-S--D-AT-EKDLSDLI-SEMEMMKMIGKHKNIINLLGACT--QDGPLYVIVEYASKGNLREYLQ-ARRPPGLEY-----------------------------------------------------------------------------------CYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTE---DNVM---------KIADFGLARDI---H-H--IDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSP---------------YPGV-PVEELFKLL-KEGHRMDKPSNCT------NELYMMMRDCWHAVPSQRPTFKQLVEDL----
-LKLEKKLGAGQFGEVWMATYN--------KHTKVAVKTMK-P--G-SM-SV--EAFL-AEANVMKTL-QHDKLVKLHAVVT---KEPIYIITEFMAKGSLLDFLK-SD-------------------------------------------------------------------------------------------------EGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSA---SLVC---------KIADFGLARVI-----E--DNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIP---------------YPGM-SNPEVIRAL-ERGYRMPRPENCP------EELYNIMMRCWKNRPEERPTFEYIQSV--L--
-ITLLRELGQGSFGMVYEGNARD--IIKGEAETRVAVKTVN-E--S-AS-LRERIEFL-NEASVMKGF-TCHHVVRLLGVVS--KGQPTLVVMELMAHGDLKSYLR-SLRPEA-----------------------------------------------------------------------------------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAH---DFTV---------KIGDFGMTRDI---Y-E--TDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQP---------------YQGL-SNEQVLKFV-MDGGYLDQPDNCP------ERVTDLMRMCWQFNPKMRPTFLEIVNLL----
-LTFVQEIGSGQFGLVHLGYWL--------NKDKVAIKTIR-E--G-AM-SE--EDFI-EEAEVMMKL-SHPKLVQLYGVCL--EQAPICLVFEFMEHGCLSDYLR-TQ--------------------------------------------------------------------------------------------------RGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGE---NQVI---------KVSDFGMTRFV-----L--DDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIP---------------YENR-SNSEVVEDI-STGFRLYKPRLAS------THVYQIMNHCWKERPEDRPAFSRLLRQL----
-LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-H--S-TE--EHLRDFE-REIEILKSL-QHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQ-KH--------------------------------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVEN---ENRV---------KIGDFGLTKVL--PQ-D--KEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM-IVFHLIELL-KNNGRLPRPDGCP------DEIYMIMTECWNNNVNQRPSFRDLA----LRV
L-KYISQLGKGNFGSVELCRYDP---LGDNTGALVAVKQLQ-H--S-GP-DQ-QRDFQ-REIQILKAL-HSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQ-RH--------------------------------------------------------------------------------------------------RARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVES---EAHV---------KIADFGLAKLL--PL-D--KDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAE--FLRMMGCERDVPALCRLLELL-EEGQRLPAPPACP------AEVHELMKLCWAPSPQDRPSFSALGP---QL-
-LSFGKTLGAGAFGKVVEATAYG--LIKSDAAMTVAVKMLK-P--S-AH-LTEREALM-SELKVLSYLGNHMNIVNLLGACT--IGGPTLVITEYCCYGDLLNFLR-RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM-----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTH---GRIT---------KICDFGLARDI---K-N--DSNYVVKGNARLPVKWMAPESIFNCVYTFESDVWSYGIFLWELFSLGSSP---------------YPGMPVDSKFYKMI-KEGFRMLSPEHAP------AEMYDIMKTCWDADPLKRPTFKQIVQL--I--
-LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q--G-SM-SP--DAFL-AEANLMKQL-QHQRLVRLYAVVT---QEPIYIITEYMENGSLVDFLK-TP-------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSD---TLSC---------KIADFGLARLI-----E--DNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIP---------------YPGM-TNPEVIQNL-ERGYRMVRPDNCP------EELYQLMRLCWKERPEDRPTFDYLRSV--L--
L-VLGRTLGSGAFGQVVEATAHG--LSHSQATMKVAVKMLK-S--T-AR-SSEKQALM-SELKIMSHLGPHLNVVNLLGACT--KGGPIYIITEYCRYGDLVDYLH-RNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICE---GKLV---------KICDFGLARDI---M-R--DSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTP---------------YPELPMNEQFYNAI-KRGYRMAQPAHAS------DEIYEIMQKCWEEKFEIRPPFSQLVL---LL-
L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P--G-TM-SP--EAFL-QEAQVMKKL-RHEKLVQLYAVVS---EEPIYIVTEYMSKGSLLDFLK-GE-------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGE---NLVC---------KVADFGLARLI-----E--DNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVP---------------YPGM-VNREVLDQV-ERGYRMPCPPECP------ESLHDLMCQCWRKEPEERPTFEYLQAF--L--
-YTFGRILGKGSFGIVIEATDK-------ETETKWAIKKVNKE--K-AGSSA--VKLLEREVNILKSV-KHEHIIHLEQVFE--TPKKMYLVMELCEDGELKEILDRKG----------------------------------------------------------------------------------------------------HFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKS---SLIDDNNEINLNIKVTDFGLAVKK----QSRSEAMLQA---TCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLR-GEPP---------------FLAS----SEEKLF-ELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWL--
-IKFQDVIGEGNFGQVLKARIK-----KDGLRMDAAIKRMK-E--Y-AS-KDDHRDFA-GELEVLCKLGHHPNIINLLGACE--HRGYLYLAIEYAPHGNLLDFLR-KSRVLETDP-----------------------------------------------------------------------------------AFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGE---NYVA---------KIADFGLSRGQ---------EVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTP---------------YCGM-TCAELYEKL-PQGYRLEKPLNCD------DEVYDLMRQCWREKPYERPSFAQILV---SL-
|