|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
CAMK CAMK1G 203
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK3 4
CMGC CDK5 4
CMGC CDK6 13
CMGC CDKL1 5
CMGC CDKL5 13
CAMK DAPK1 13
CAMK DAPK3 13
Other NEK1 4
Other NEK2 8
Other NEK4 6
Other NEK5 4
AGC PDPK1 82
CAMK PHKG1 20
CAMK PHKG2 24
CAMK PIM1 38
CAMK PIM3 40
AGC RPS6KA1 62
AGC RPS6KA2 59
AGC RPS6KA3 68
AGC RPS6KA6 73
STE STK24 24
CAMK STK33 116
Other TTK 525
Other ULK3 14
-FI--FMEVLGSGAFSEV---FLVKQR-LTGK---LFALKCIKKSPAFRDSSL-E--N-------EIAVLKKI-K----HEN--IVTLEDIYES-----TTHYYLVMQLVSGG-ELFDRIL----ERGVYTEKDASLVIQQVLSAVKYLHEN-----GIVHRDLKPENLLYLTPEENSKI---------MITDFGLSK----MEQNGIMSTACGTPGYVAPEVLA-----------QKPYSKAVDCWSIGVITYILLC-GYPPFYEE---TESKLFEKIKEGYYEFESPFWDDIS-------------------------ESAKDFICHLLEKDPNERYT-----CEKALSHPWI-----------
---YTKIEKIGEGTYGVV---YKGRHK-TTGQ---VVAMKKIRLESEEEGVPS-TAIR-------EISLLKEL-R----HPN--IVSLQDVLMQ-----DSRLYLIFEFLSM--DLKKYLDS-IPPGQYMDSSLVKSYLYQILQGIVFCHSR-----RVLHRDLKPQNLLID---DKGTI---------KLADFGLARAF--GIPIRVYTHEVVTLWYRSPEVLL----------GSARYSTPVDIWSIGTIFAELAT-KKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKP---GSLASHVKNLDENGLDLLSKMLIYDPAKRIS-----GKMALNHPYF-----------
---FQKVEKIGEGTYGVV---YKARNK-LTGE---VVALKKIRLDTETEGVPS-TAIR-------EISLLKEL-N----HPN--IVKLLDVIHT-----ENKLYLVFEFLHQ--DLKKFMDA--SALTGIPLPLIKSYLFQLLQGLAFCHSH-----RVLHRDLKPQNLLIN---TEGAI---------KLADFGLARAF--GVPVRTYTHEVVTLWYRAPEILL----------GCKYYSTAVDIWSLGCIFAEMVT-RRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWAR---QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-----AKAALAHPFF-----------
---FQKVEKIGEGTYGVV---YKAKNR-ETGQ---LVALKKIRLDLEMEGVPS-TAIR-------EISLLKEL-K----HPN--IVRLLDVVHN-----ERKLYLVFEFLSQ--DLKKYMDS--TPGSELPLHLIKSYLFQLLQGVSFCHSH-----RVIHRDLKPQNLLIN---ELGAI---------KLADFGLARAF--GVPLRTYTHEVVTLWYRAPEILL----------GSKFYTTAVDIWSIGCIFAEMVT-RKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTR---KGLEEIVPNLEPEGRDLLMQLLQYDPSQRIT-----AKTALAHPYF-----------
---YEKLEKIGEGTYGTV---FKAKNR-ETHE---IVALKRVRLDDDDEGVPS-SALR-------EICLLKEL-K----HKN--IVRLHDVLHS-----DKKLTLVFEFCDQ--DLKKYFD---SCNGDLDPEIVKSFLFQLLKGLGFCHSR-----NVLHRDLKPQNLLIN---RNGEL---------KLADFGLARAF--GIPVRCYSAEVVTLWYRPPDVLF----------GAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPAT---TSLVNVVPKLNATGRDLLQNLLKCNPVQRIS-----AEEALQHPYF-----------
---YECVAEIGEGAYGKV---FKARDL-KNGG--RFVALKRVRVQTGEEGMPL-STIR-------EVAVLRHL-ET-FEHPN--VVRLFDVCTVSRTDRETKLTLVFEHVDQ--DLTTYLDK--VPEPGVPTETIKDMMFQLLRGLDFLHSH-----RVVHRDLKPQNILVT---SSGQI---------KLADFGLARI---YSFQMALTSVVVTLWYRAPEVLL-----------QSSYATPVDLWSVGCIFAEMFR-RKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQ-----PIEKFVTDIDELGKDLLLKCLTFNPAKRIS-----AYSALSHPYF-----------
---YEKIGKIGEGSYGVV---FKCRNR-DTGQ---IVAIKKFLESEDDPVIKK-IALR-------EIRMLKQL-K----HPN--LVNLLEVFRR-----KRRLHLVFEYCDHT-VLHELDR----YQRGVPEHLVKSITWQTLQAVNFCHKH-----NCIHRDVKPENILIT---KHSVI---------KLCDFGFARLL--AGPSDYYTDYVATRWYRSPELLV----------GDTQYGPPVDVWAIGCVFAELLS-GVPLWPGKSDVDQLYLIRKTLGDLIPRHQQVFSTNQYFSGVKIPDPED-MEPLELKFPNISYPALGLLKGCLHMDPTQRLT-----CEQLLHHPYF-----------
---FEILGVVGEGAYGVV---LKCRHK-ETHE---IVAIKKFKDSEENEEVKE-TTLR-------ELKMLRTL-K----QEN--IVELKEAFRR-----RGKLYLVFEYVEK--NMLELLE---EMPNGVPPEKVKSYIYQLIKAIHWCHKN-----DIVHRDIKPENLLIS---HNDVL---------KLCDFGFARNL-SEGNNANYTEYVATRWYRSPELLL-----------GAPYGKSVDMWSVGCILGELSD-GQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYL-----TEQCLNHPTF-----------
---YDTGEELGSGQFAVV---KKCREK-STGL---QYAAKFIKKRRTKSSRRG-VSREDI---EREVSILKEI-Q----HPN--VITLHEVYEN-----KTDVILILELVAGG-ELFDFLA----EKESLTEEEATEFLKQILNGVYYLHSL-----QIAHFDLKPENIMLL---DRNVPKP-----RIKIIDFGLAHK---IDFGNEFKNIFGTPEFVAPEIVN-----------YEPLGLEADMWSIGVITYILLS-GASPFLGD---TKQETLANVSAVNYEFEDEYFSNTS-------------------------ALAKDFIRRLLVKDPKKRMT-----IQDSLQHPWI-----------
---YEMGEELGSGQFAIV---RKCRQK-GTGK---EYAAKFIKKRRLSSSRRG-VSREEI---EREVNILREI-R----HPN--IITLHDIFEN-----KTDVVLILELVSGG-ELFDFLA----EKESLTEDEATQFLKQILDGVHYLHSK-----RIAHFDLKPENIMLL---DKNVPNP-----RIKLIDFGIAHK---IEAGNEFKNIFGTPEFVAPEIVN-----------YEPLGLEADMWSIGVITYILLS-GASPFLGE---TKQETLTNISAVNYDFDEEYFSNTS-------------------------ELAKDFIRRLLVKDPKRRMT-----IAQSLEHSWI-----------
--Y-VRLQKIGEGSFGKA---ILVKST-EDGR---QYVIKEINISRMSSKERE-ESRR-------EVAVLANM-K----HPN--IVQYRESFEE-----NGSLYIVMDYCEGG-DLFKRINA--QKGVLFQEDQILDWFVQICLALKHVHDR-----KILHRDIKSQNIFLT---KDGTV---------QLGDFGIARVL--NSTVELARTCIGTPYYLSPEICE-----------NKPYNNKSDIWALGCVLYELCT-LKHAFEAG---SMKNLVLKIISGSFPPVSLHYS----------------------------YDLRSLVSQLFKRNPRDRPS-----VNSILEKGF-I----------
---YEVLYTIGTGSYGRC---QKIRRK-SDGK---ILVWKELDYGSMTEAEKQ-MLVS-------EVNLLREL-K----HPN--IVRYYDRIIDR---TNTTLYIVMEYCEGG-DLASVITKGTKERQYLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLD---GKQNV---------KLGDFGLARIL--NHDTSFAKTFVGTPYYMSPEQMN-----------RMSYNEKSDIWSLGCLLYELCA-LMPPFTAF---SQKELAGKIREGKFRRIPYRY---S-------------------------DELNEIITRMLNLKDYHRPS-----VEEILENPL-I----------
--Y-CYLRVVGKGSYGEV---TLVKHR-RDGK---QYVIKKLNLRNASSRERR-AAEQ-------EAQLLSQL-K----HPN--IVTYKESWEG----GDGLLYIVMGFCEGG-DLYRKLKE--QKGQLLPENQVVEWFVQIAMALQYLHEK-----HILHRDLKTQNVFLT---RTNII---------KVGDLGIARVL--ENHCDMASTLIGTPYYMSPELFS-----------NKPYNYKSDVWALGCCVYEMAT-LKHAFNAK---DMNSLVYRIIEGKLPPMPRDY---S-------------------------PELAELIRTMLSKRPEERPS-----VRSILRQPYI-----------
---YDVIKAIGQGAFGKA---YLAKGK-SDSK---HCVIKEINFEKMPIQEKE-ASKK-------EVILLEKM-K----HPN--IVAFFNSFQE-----NGRLFIVMEYCDGG-DLMKRINR--QRGVLFSEDQILGWFVQISLGLKHIHDR-----KILHRDIKAQNIFLS---KNGMV--------AKLGDFGIARVL--NNSMELARTCIGTPYYLSPEICQ-----------NKPYNNKTDIWSLGCVLYELCT-LKHPFEGN---NLQQLVLKICQAHFAPISPGF---S-------------------------RELHSLISQLFQVSPRDRPS-----INSILKRPFL-----------
--F-KFGKILGEGSFSTV---VLAREL-ATSR---EYAIKILEKRHIIKENKVPYVTR-------ERDVMSRL-D----HPF--FVKLYFTFQD-----DEKLYFGLSYAKNG-ELLKYIR----KIGSFDETCTRFYTAEIVSALEYLHGK-----GIIHRDLKPENILLN---EDMHI---------QITDFGTAKVLSPESKQARANSFVGTAQYVSPELLT-----------EKSACKSSDLWALGCIIYQLVA-GLPPFRAG---NEYLIFQKIIKLEYDFPEKFF-----------------------------PKARDLVEKLLVLDATKRLG-----CEEM------EGYGPLKAHPF
---YEPKEILGRGVSSVV---RRCIHK-PTSQ---EYAVKVIDVTGGGSFSPE-EVRELREATLKEVDILRKVSG----HPN--IIQLKDTYET-----NTFFFLVFDLMKRG-ELFDYLT----EKVTLSEKETRKIMRALLEVICTLHKL-----NIVHRDLKPENILLD---DNMNI---------KLTDFGFSCQ---LEPGERLREVCGTPSYLAPEIIECS-----MNEDHPGYGKEVDMWSTGVIMYTLLA-GSPPFWHR---KQMLMLRMIMSGNYQFGSPEWDDYS-------------------------DTVKDLVSRFLVVQPQNRYT-----AEEALAHPFF-----------
YDP---KDVIGRGVSSVV---RRCVHR-ATGH---EFAVKIMEVTAERLSPEQ-LEEVREA-TRRETHILRQVAG----HPH--IITLIDSYES-----SSFMFLVFDLMRKG-ELFDYLT----EKVALSEKETRSIMRSLLEAVSFLHAN-----NIVHRDLKPENILLD---DNMQI---------RLSDFGFSCH---LEPGEKLRELCGTPGYLAPEILKCS-----MDETHPGYGKEVDLWACGVILFTLLA-GSPPFWHR---RQILMLRMIMEGQYQFSSPEWDDRS-------------------------STVKDLISRLLQVDPEARLT-----AEQALQHPFF-----------
---YQVGPLLGSGGFGSV---YSGIRV-SDNL---PVAIKHVEKDRISDWGEL-PNGTRV---PMEVVLLKKV-S----SGFSGVIRLLDWFER-----PDSFVLILERPEPVQDLFDFIT----ERGALQEELARSFFWQVLEAVRHCHNC-----GVLHRDIKDENILID--LNRGEL---------KLIDFGSGA----LLKDTVYTDFDGTRVYSPPEWIR----------YHRYHGRSAAVWSLGILLYDMVC-GDIPFEHD---------EEIIRGQVFFRQRVS-----------------------------SECQHLIRWCLALRPSDRPT-----FEEIQNHPWM-----------
---YQVGAVLGSGGFGTV---YAGSRI-ADGL---PVAVKHVVKERVTEWGSL-GGATV----PLEVVLLRKV-GAAGGARG--VIRLLDWFER-----PDGFLLVLERPEPAQDLFDFIT----ERGALDEPLARRFFAQVLAAVRHCHSC-----GVVHRDIKDENLLVD--LRSGEL---------KLIDFGSGA----LLKDTVYTDFDGTRVYSPPEWIR----------YHRYHGRSATVWSLGVLLYDMVC-GDIPFEQD---------EEILRGRLLFRRRVS-----------------------------PECQQLIRWCLSLRPSERPS-----LDQIAAHPWM-----------
---FELLKVLGQGSFGKVFLVRKVTRP-DSGH---LYAMKVLKKATLKVRDRV-RTKM-------ERDILADV-N----HPF--VVKLHYAFQT-----EGKLYLILDFLRGG-DLFTRLS----KEVMFTEEDVKFYLAELALGLDHLHSL-----GIIYRDLKPENILLD---EEGHI---------KLTDFGLSKEA--IDHEKKAYSFCGTVEYMAPEVVN-----------RQGHSHSADWWSYGVLMFEMLT-GSLPFQGK---DRKETMTLILKAKLGMPQFLS-----------------------------TEAQSLLRALFKRNPANRLGSGPDGAEEIKRHV--FY---------
---FELLKVLGQGSYGKV---FLVRKV-KGSDAGQLYAMKVLKKATLKVRDRV-RSKM-------ERDILAEV-N----HPF--IVKLHYAFQT-----EGKLYLILDFLRGG-DLFTRLS----KEVMFTEEDVKFYLAELALALDHLHSL-----GIIYRDLKPENILLD---EEGHI---------KITDFGLSKEA--IDHDKRAYSFCGTIEYMAPEVVN-----------RRGHTQSADWWSFGVLMFEMLT-GSLPFQGK---DRKETMALILKAKLGMPQFLS-----------------------------GEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFF-----------
---FELLKVLGQGSFGKV---FLVKKI-SGSDARQLYAMKVLKKATLKVRDRV-RTKM-------ERDILVEV-N----HPF--IVKLHYAFQT-----EGKLYLILDFLRGG-DLFTRLS----KEVMFTEEDVKFYLAELALALDHLHSL-----GIIYRDLKPENILLD---EEGHI---------KLTDFGLSKES--IDHEKKAYSFCGTVEYMAPEVVN-----------RRGHTQSADWWSFGVLMFEMLT-GTLPFQGK---DRKETMTMILKAKLGMPQFLS-----------------------------PEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHSFF-----------
---FELLKVLGQGSFGKV---FLVRKK-TGPDAGQLYAMKVLKKASLKVRDRV-RTKM-------ERDILVEV-N----HPF--IVKLHYAFQT-----EGKLYLILDFLRGG-DVFTRLS----KEVLFTEEDVKFYLAELALALDHLHQL-----GIVYRDLKPENILLD---EIGHI---------KLTDFGLSKES--VDQEKKAYSFCGTVEYMAPEVVN-----------RRGHSQSADWWSYGVLMFEMLT-GTLPFQGK---DRNETMNMILKAKLGMPQFLS-----------------------------AEAQSLLRMLFKRNPANRLGS--EGVEEIKRHLFF-----------
---FTKLEKIGKGSFGEV---FKGIDN-RTQK---VVAIKIIDLEEAEDEIED-IQ-Q-------EITVLSQC-D----SPY--VTKYYGSYLK-----DTKLWIIMEYLGGG-SALDLLE-----PGPLDETQIATILREILKGLDYLHSE-----KKIHRDIKAANVLLS---EHGEV---------KLADFGVAGQL--TDTQIKRNTFVGTPFWMAPEVIK-----------QSAYDSKADIWSLGITAIELAR-GEPPHSELHPMKVLFLIPKNNPPTLEGNYS-------------------------------KPLKEFVEACLNKEPSFRPT-----AKELLKHKFI-----------
---YTFGRILGKGSFGIV---IEATDK-ETET---KWAIKKVNKEKAGSSAVK-LLER-------EVNILKSV-K----HEH--IIHLEQVFET-----PKKMYLVMELCEDG-ELKEILD----RKGHFSENETRWIIQSLASAIAYLHNN-----DIVHRDLKLENIMVK---SSLIDDNNEINLNIKVTDFGLAVKK-QSRSEAMLQATCGTPIYMAPEVIS-----------AHDYSQQCDIWSIGVVMYMLLR-GEPPFLAS---SEEKLFELIRKGELHFENAVWNSIS-------------------------DCAKSVLKQLMKVDPAHRIT-----AKELLDNQWL-----------
---YSILKQIGSGGSSKV---FQVLNE-KKQ----IYAIKYVNLEEADNQTLD-SYRN-------EIAYLNKLQQ---HSDK--IIRLYDYEIT-----DQYIYMVMECGNI--DLNSWLK----KKKSIDPWERKSYWKNMLEAVHTIHQH-----GIVHSDLKPANFLIV----DGML---------KLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTY-GKTPFQQI--INQISKLHAIIDPNHEIEFPDIPE---------------------------KDLQDVLKCCLKRDPKQRIS-----IPELLAHPYV-----------
-FI--LTERLGSGTYATV---YKAYAKKDTRE---VVAIKCVAKKSLNKASVE-NLLT-------EIEILKGI-R----HPH--IVQLKDFQWD-----SDNIYLIMEFCAGG-DLSRFIH----TRRILPEKVARVFMQQLASALQFLHER-----NISHLDLKPQNILLSS-LEKPHL---------KLADFGFAQH---MSPWDEKHVLRGSPLYMAPEMVC-----------QRQYDARVDLWSMGVILYEALF-GQPPFASRSFSELEEKIRSNRVIELPLRPLLS-----------------------------RDCRDLLQRLLERDPSRRIS-----FQDFFAHPWV-----------
|