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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
AGC PRKCD 203
TK ABL1 261
TK BTK 402
CMGC CDK2 4
CMGC CDK5 4
CAMK CHEK1 9
TK EGFR 712
TK EPHB4 615
TK FGFR3 472
TK HCK 241
TK IGF1R 999
TKL IRAK4 186
TK JAK2 849
TK JAK3 822
TK KIT 589
TK LCK 245
TK PDGFRA 593
AGC PRKCQ 380
TK PTK2 422
TKL RAF1 349
TK ROS1 1945
TK SRC 270
TK TNK2 150
TK ZAP70 338
--FI--FHKVLGKGSFGKVLLGELK-------GRGEYFAIKALKKD--VVLIDD--D-VECTMVEKRVLTLA-AENPFLTHLICTFQ--TKDHLFFVMEFLNGGDLMYHIQ---DKG---------------------------------------------------------------------------------------------------RFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDR-----DGHIKIADFGMCKEN-----IFGESRAST---FCGTPDYIAPEILQ-GLK---YTFSVDWWSFGVLLYEMLI-GQSP---------------FHGD----DEDELF-ESIRV----DTPHYPRWIT--------------------------KESKDILEKLFEREPTKRLGVTG-NIKIHPFF---
----ITMKHKLGGGQYGEVYEGVWK-------KYSLTVAVKTLK-E--DTM-EV--E-EFL-K-EAAVMKEI--KHPNLVQLLGVCT--REPPFYIITEFMTYGNLLDYLR----EC------------------------------------------------------------------------------------------------NRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGE-----NHLVKVADFGLSRLM-----T--GDTYTAHAGAKFPIKWTAPESLA-YNK---FSIKSDVWAFGVLLWEIATYGMSP---------------YPGI-DLSQVYELL-EKDYR----MERPEGCP----------------------------EKVYELMRACWQWNPSDRPS-FA-EIHQA--F---
----LTFLKELGTGQFGVVKYGKWR--------GQYDVAIKMIK-E--GSM-SE--D-EFI-E-EAKVMMNL--SHEKLVQLYGVCT--KQRPIFIITEYMANGCLLNYLR----EM-------------------------------------------------------------------------------------------------RHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVND-----QGVVKVSDFGLSRYV-----L--DDEYTSSVGSKFPVRWSPPEVLM-YSK---FSSKSDIWAFGVLMWEIYSLGKMP---------------YERF-TNSETAEHI-AQGLR----LYRPHLAS----------------------------EKVYTIMYSCWHEKADERPT-FK-ILLSN--I---
----FQKVEKIGEGTYGVVYKARNK-------LTGEVVALKKIRLD--TETEGV--P-STAIR-EISLLKEL--NHPNIVKLLDVIH--TENKLYLVFEFLHQ-DLKKFMDA-SALT---------------------------------------------------------------------------------------------------GIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINT-----EGAIKLADFGLARAF-----GVPVRTYTH---EVVTLWYRAPEILLGCKY---YSTAVDIWSLGCIFAEMVT-RRAL---------------FPGD-SEIDQLFRI-FRTLG----TPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-AK-AALAHPFF---
----YEKLEKIGEGTYGTVFKAKNR-------ETHEIVALKRVRLD--DDDEGV--P-SSALR-EICLLKEL--KHKNIVRLHDVLH--SDKKLTLVFEFCDQ-DLKKYFD--SCNG---------------------------------------------------------------------------------------------------DLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINR-----NGELKLADFGLARAF-----GIPVRCYSA---EVVTLWYRPPDVLFGAKL---YSTSIDMWSAGCIFAELANAGRPL---------------FPGN-DVDDQLKRI-FRLLG----TPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRIS-AE-EALQHPYF---
---W-DLVQTLGEGAYGEVQLAVNR-------VTEEAVAVKIVDMK--RAVDCP--E-NI-KK-EICINKML--NHENVVKFYGHRR--EGNIQYLFLEYCSGGELFDRIE---PDI---------------------------------------------------------------------------------------------------GMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDE-----RDNLKISDFGLATVFR---YNNRERLLNK---MCGTLPYVAPELLKRREF---HAEPVDVWSCGIVLTAMLA-GELP---------------WDQP---SDSCQEY-SDWKE----KKTYLNPWKKI--D----------------------SAPLALLHKILVENPSARIT-IP-DIKKDRW-Y--
---F-KKIKVLGSGAFGTVYKGLWIP---EGEKVKIPVAIKELR-E--ATS-PKANK-EIL-D-EAYVMASV--DNPHVCRLLGICL---TSTVQLITQLMPFGCLLDYVR----EH-------------------------------------------------------------------------------------------------KDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKT-----PQHVKITDFGLAKLL---G-A--EEKEYHAEGGKVPIKWMALESIL-HRI---YTHQSDVWSYGVTVWELMTFGSKP---------------YDGI-PASEISSIL-EKGER----LPQPPICT----------------------------IDVYMIMVKCWMIDADSRPK-FR-ELI----IEF-
---V-KIEEVIGAGEFGEVCRGRLK----APGKKESCVAIKTLK-G--GYT-ERQRR-EFL-S-EASIMGQF--EHPNIIRLEGVVT--NSMPVMILTEFMENGALDSFLR----LN-------------------------------------------------------------------------------------------------DGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNS-----NLVCKVSDFGLSRFLEENS-S--DPTYTSSLGGKIPIRWTAPEAIA-FRK---FTSASDAWSYGIVMWEVMSFGERP---------------YWDM-SNQDVINAI-EQDYR----LPPPPDCP----------------------------TSLHQLMLDCWQKDRNARPR-FP-QVVSAL-----
----LTLGKPLGEGCFGQVVMAEAIGIDKDRAAKPVTVAVKMLK-D--DAT-DKDLS-DLV-S-EMEMMKMIG-KHKNIINLLGACT--QGGPLYVLVEYAAKGNLREFLR----ARRPPGLDY----------------------------------------------------------------------------------SFDTCKPPEEQLTFKDLVSCAYQVARGMEYLASQKCIHRDLAARNVLVTE-----DNVMKIADFGLARDV---H-N--LDYYKKTTNGRLPVKWMAPEALF-DRV---YTHQSDVWSFGVLLWEIFTLGGSP---------------YPGI-PVEELFKLL-KEGHR----MDKPANCT----------------------------HDLYMIMRECWHAAPSQRPT-FK-QLVEDL-----
----LKLEKKLGAGQFGEVWMATYN--------KHTKVAVKTMK-P--GSM-SV--E-AFL-A-EANVMKTL--QHDKLVKLHAVVT---KEPIYIITEFMAKGSLLDFLK----SD------------------------------------------------------------------------------------------------EGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSA-----SLVCKIADFGLARVI-----E--DNEYTAREGAKFPIKWTAPEAIN-FGS---FTIKSDVWSFGILLMEIVTYGRIP---------------YPGM-SNPEVIRAL-ERGYR----MPRPENCP----------------------------EELYNIMMRCWKNRPEERPT-FE-YIQSV--L---
----ITMSRELGQGSFGMVYEGVAKG--VVKDEPETRVAIKTVN-E--AAS-MRERI-EFL-N-EASVMKEF--NCHHVVRLLGVVS--QGQPTLVIMELMTRGDLKSYLR----SLRPEM----------------------------------------------------------------------------------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAE-----DFTVKIGDFGMTRDI---Y-E--TDYYRKGGKGLLPVRWMSPESLK-DGV---FTTYSDVWSFGVVLWEIATLAEQP---------------YQGL-SNEQVLRFV-MEGGL----LDKPDNCP----------------------------DMLFELMRMCWQYNPKMRPS-FL-EIISS--I---
-VGG----NKMGEGGFGVVYKGYVN-------NTT--VAVKKLAAM--VDITTE--ELKQQFDQEIKVMAKC--QHENLVELLGFSS--DGDDLCLVYVYMPNGSLLDRLSCLDGTP---------------------------------------------------------------------------------------------------PLSWHMRCKIAQGAANGINFLHENHHIHRDIKSANILLDE-----AFTAKISDFGLARASE---KFAQTVMTSR---IVGTTAYMAPEALR--GE---ITPKSDIYSFGVVLLEIIT-GLPA---------------VDEH-REPQLLLDI-KEEIE----DEEKTIEDYIDKKMNDAD--------------STSVEAMYSVASQCLHEKKNKRPD-IK-KVQQ----LLQ
----LKFLQQLGKGNFGSVEMCRYDP---LQDNTGEVVAVKKLQ-H--STE--EHLR-DFE-R-EIEILKSL--QHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQ----KH-------------------------------------------------------------------------------------------------KERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVEN-----ENRVKIGDFGLTKVL--PQ-D--KEYYKVKEPGESPIFWYAPESLT-ESK---FSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQM-IVFHLIELL-KNNGR----LPRPDGCP----------------------------DEIYMIMTECWNNNVNQRPS-FR-DLA----LRV-
---L-KYISQLGKGNFGSVELCRYDP---LGDNTGALVAVKQLQ-H--SGP-DQ-QR-DFQ-R-EIQILKAL--HSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQ----RH-------------------------------------------------------------------------------------------------RARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVES-----EAHVKIADFGLAKLL--PL-D--KDYYVVREPGQSPIFWYAPESLS-DNI---FSRQSDVWSFGVVLYELFTYCDKSCSPSAE--FLRMMGCERDVPALCRLLELL-EEGQR----LPAPPACP----------------------------AEVHELMKLCWAPSPQDRPS-FS-ALGP---QL--
----LSFGKTLGAGAFGKVVEATAYG--LIKSDAAMTVAVKMLK-P--SAH-LTERE-ALM-S-ELKVLSYLG-NHMNIVNLLGACT--IGGPTLVITEYCCYGDLLNFLR----RKRDSFICSKQEDHAEAALYKNLLHSKESSCSDSTNEYM----------------------DMKPGVSYVVPTKADKRRSVRIGSYIERDVTPAIMEDDELALDLEDLLSFSYQVAKGMAFLASKNCIHRDLAARNILLTH-----GRITKICDFGLARDI---K-N--DSNYVVKGNARLPVKWMAPESIF-NCV---YTFESDVWSYGIFLWELFSLGSSP---------------YPGMPVDSKFYKMI-KEGFR----MLSPEHAP----------------------------AEMYDIMKTCWDADPLKRPT-FK-QIVQL--I---
----LKLVERLGAGQFGEVWMGYYN--------GHTKVAVKSLK-Q--GSM-SP--D-AFL-A-EANLMKQL--QHQRLVRLYAVVT---QEPIYIITEYMENGSLVDFLK----TP------------------------------------------------------------------------------------------------SGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSD-----TLSCKIADFGLARLI-----E--DNEYTAREGAKFPIKWTAPEAIN-YGT---FTIKSDVWSFGILLTEIVTHGRIP---------------YPGM-TNPEVIQNL-ERGYR----MVRPDNCP----------------------------EELYQLMRLCWKERPEDRPT-FD-YLRSV--L---
---L-VLGRVLGSGAFGKVVEGTAYG--LSRSQPVMKVAVKMLK-P--TAR-SSEKQ-ALM-S-ELKIMTHLG-PHLNIVNLLGACT--KSGPIYIITEYCFYGDLVNYLH----KNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQ-----GKIVKICDFGLARDI---M-H--DSNYVSKGSTFLPVKWMAPESIF-DNL---YTTLSDVWSYGILLWEIFSLGGTP---------------YPGMMVDSTFYNKI-KSGYR----MAKPDHAT----------------------------SEVYEIMVKCWNSEPEKRPS-FY-HLSE---IV--
--FI--LHKMLGKGSFGKVFLAEFK-------KTNQFFAIKALKKD--VVLMDD--D-VECTMVEKRVLSLA-WEHPFLTHMFCTFQ--TKENLFFVMEYLNGGDLMYHIQ---SCH---------------------------------------------------------------------------------------------------KFDLSRATFYAAEIILGLQFLHSKGIVYRDLKLDNILLDK-----DGHIKIADFGMCKEN-----MLGDAKTNT---FCGTPDYIAPEILL-GQK---YNHSVDWWSFGVLLYEMLI-GQSP---------------FHGQ----DEEELF-HSIRM----DNPFYPRWLE--------------------------KEAKDLLVKLFVREPEKRLG-VRGDIRQHPLF---
----IELGRCIGEGQFGDVHQGIYM----SPENPALAVAIKTCK-N--CTS-DSVRE-KFL-Q-EALTMRQF--DHPHIVKLIGVIT---ENPVWIIMELCTLGELRSFLQ----VR-------------------------------------------------------------------------------------------------KYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSS-----NDCVKLGDFGLSRYM-----E--DSTYYKASKGKLPIKWMAPESIN-FRR---FTSASDVWMFGVCMWEILMHGVKP---------------FQGV-KNNDVIGRI-ENGER----LPMPPNCP----------------------------PTLYSLMTKCWAYDPSRRPR-FT-ELKAQL-----
--VM--LSTRIGSGSFGTVYKGKWH----------GDVAVKILK-V-VDPT-PEQFQ-AFR-N-EVAVLRKT--RHVNILLFMGYMT---KDNLAIVTQWCEGSSLYKHLH----VQ-------------------------------------------------------------------------------------------------ETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE-----GLTVKIGDFGLATVK---S-R--WSGSQQVEQPTGSVLWMAPEVIR-MQDNNPFSFQSDVYSYGIVLYELMT-GELP---------------YSHINNRDQIIFMV-GRGYASPDLSKLYKNCP----------------------------KAMKRLVADCVKKVKEERPL-FP-QILSS--I---
--LT--LRLLLGSGAFGEVYEGTAVDI-LGVGSGEIKVAVKTLK-K--GST-DQEKI-EFL-K-EAHLMSKF--NHPNILKQLGVCL--LNEPQYIILELMEGGDLLTYLR----KARM-------------------------------------------------------------------------------------------ATFYGPLLTLVDLVDLCVDISKGCVYLERMHFIHRDLAARNCLVSVKDYTSPRIVKIGDFGLARDI---Y-K--NDYYRKRGEGLLPVRWMAPESLM-DGI---FTTQSDVWSFGILIWEILTLGHQP---------------YPAH-SNLDVLNYV-QTGGR----LEPPRNCP----------------------------DDLWNLMTQCWAQEPDQRPT-FH-RIQDQL-----
---L-RLEVKLGQGCFGEVWMGTWN--------GTTRVAIKTLK-P--GTM-SP--E-AFL-Q-EAQVMKKL--RHEKLVQLYAVVS---EEPIYIVTEYMSKGSLLDFLK----GE------------------------------------------------------------------------------------------------TGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGE-----NLVCKVADFGLARLI-----E--DNEYTARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELTTKGRVP---------------YPGM-VNREVLDQV-ERGYR----MPCPPECP----------------------------ESLHDLMCQCWRKEPEERPT-FE-YLQAF--L---
---L-RLLEKLGDGSFGVVRRGEWD----APSGKTVSVAVKCLK-PDVLSQ-PEAMD-DFI-R-EVNAMHSL--DHRNLIRLYGVVL---TPPMKMVTELAPLGSLLDRLR----KH-------------------------------------------------------------------------------------------------QGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLAT-----RDLVKIGDFGLMRAL--PQ-N--DDHYVMQEHRKVPFAWCAPESLK-TRT---FSHASDTWMFGVTLWEMFTYGQEP---------------WIGL-NGSQILHKIDKEGER----LPRPEDCP----------------------------QDIYNVMVQCWAHKPEDRPT-FV-ALRDF--L---
LIAD----IELGCGNFGSVRQGVYR-----MRKKQIDVAIKVLK-Q--GTE-KADTE-EMM-R-EAQIMHQL--DNPYIVRLIGVCQ---AEALMLVMEMAGGGPLHKFLV----GK-------------------------------------------------------------------------------------------------REEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVN-----RHYAKISDFGLSKAL--GA-D--DSYYTARSAGKWPLKWYAPECIN-FRK---FSSRSDVWSYGVTMWEALSYGQKP---------------YKKM-KGPEVMAFI-EQGKR----MECPPECP----------------------------PELYALMSDCWIYKWEDRPD-FL-TVEQR--M---
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