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Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
AGC PRKCZ 203
AGC AKT1 150
AGC AKT3 148
TKL BRAF 456
CMGC CDC2 4
CMGC CDK2 4
CMGC CDK4 6
CMGC CDK5 4
CAMK CHEK1 9
CMGC GSK3B 56
TK LYN 247
CMGC MAPK10 64
CMGC MAPK14 24
CAMK MAPKAPK2 64
AGC PRKCA 339
AGC PRKCB 342
AGC PRKCD 349
AGC PRKCE 408
AGC PRKCH 355
AGC PRKCQ 380
TK SRC 270
--F-DLIRVIGRGSYAKVLLVRLKKNDQIYAMKVVKKE-LVHD---DE--DIDWVQTEKHVFEQASS---NPFLVGLHSCFQT------TSRLF--LVIEYVNGG--DLMFHMQ----RQR---KLPEEHARFYAAEICIALNFLHE-RGIIYRDLKLDNVLLD-ADGHI---KLTDYGMCKEG---LGPGDTTST---FCGTPNYIAPEILR-GEE---YGFSVDWWALGVLMFEMMA-GRSPFDIITDNPDMNTEDYLFQVILE----KPIRIPRFLS---------------------------------------VKASHVLKGFLNKDPKERLGCRP-QTGF----------SDIKSHAFF-----
--F-EYLKLLGKGTFGKVILVKEKATGRYYAMKILKKE-VIVA---KD--EVAHTLTENRVLQNS-R---HPFLTALKYSFQT------HDRLC--FVMEYANGG--ELFFHLS----RER---VFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLD-KDGHI---KITDFGLCKEG---IKDGATMKT---FCGTPEYLAPEVLE-DND---YGRAVDWWGLGVVMYEMMC-GRLPFYNQ-------DHEKLFELILM----EEIRFPRTLG---------------------------------------PEAKSLLSGLLKKDPKQRLGGG--SEDAK-EIMQHRFF--------------
-FD--YLKLLGKGTFGKVILVREKASGKYYAMKILKKE-VIIA---KD--EVAHTLTESRVLKNT-R---HPFLTSLKYSFQT------KDRLC--FVMEYVNGG--ELFFHLS----RER---VFSEDRTRFYGAEIVSALDYLHS-GKIVYRDLKLENLMLD-KDGHI---KITDFGLCKEG---ITDAATMKT---FCGTPEYLAPEVLE-DND---YGRAVDWWGLGVVMYEMMC-GRLPFYNQ-------DHEKLFELILM----EDIKFPRTLS---------------------------------------SDAKSLLSGLLIKDPNKRLGGG--PDDAK-EIMRHSFF--------------
---ITVGQRIGSGSFGTVYKGKWH---GDVAVKMLN-VTAPT----PQQLQAFK-N-EVGVLRKT-R---HVNILLFMGYST-------KPQLA--IVTQWCEGS--SLYHHLH-----II-ETKFEMIKLIDIARQTAQGMDYLHA-KSIIHRDLKSNNIFLH-EDLTV---KIGDFGLATVK-S-R--WSGSHQFEQLSGSILWMAPEVIR-MQDKNPYSFQSDVYAFGIVLYELMT-GQLPYSNI-N--NRDQIIFMVGRGYLSPDLSKVRSNCP-----------------------------------------KAMKRLMAECLKKKRDERPL-------FP-QILA---SI-------------
---YTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLE-SEEE---GV--PSTAIR-EISLLKEL-R---HPNIVSLQDVLMQ------DSRLY--LIFEFLSM---DLKKYLDS-IPPGQ---YMDSSLVKSYLYQILQGIVFCHS-RRVLHRDLKPQNLLID-DKGTI---KLADFGLARAF---GIPIRVYTH---EVVTLWYRSPEVLLGSAR---YSTPVDIWSIGTIFAELAT-KKPLFHGD----SEIDQLFRIFRALG----TPNNEVWPEVESLQDYKNTFPKWKP-------------GSLASHVKNLDENGLDLLSKMLIYDPAKRIS-------GK-MALNHPYF--------------
---FQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLD-TETE---GV--PSTAIR-EISLLKEL-N---HPNIVKLLDVIHT------ENKLY--LVFEFLHQ---DLKKFMDA--SALT---GIPLPLIKSYLFQLLQGLAFCHS-HRVLHRDLKPQNLLIN-TEGAI---KLADFGLARAF---GVPVRTYTH---EVVTLWYRAPEILLGCKY---YSTAVDIWSLGCIFAEMVT-RRALFPGD----SEIDQLFRIFRTLG----TPDEVVWPGVTSMPDYKPSFPKWAR-------------QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS-------AK-AALAHPFF--------------
--Y-EPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVP-NGGGGGGGL--PISTVR-EVALLRRL-EAFEHPNVVRLMDVCATSR-TDREIKVT--LVFEHVDQ---DLRTYLDK--APPP---GLPAETIKDLMRQFLRGLDFLHA-NCIVHRDLKPENILVT-SGGTV---KLADFGLARI----YSYQMALTP---VVVTLWYRAPEVLL-QST---YATPVDMWSVGCIFAEMFR-RKPLFCGN----SEADQLGKIFDLIG----LPPEDDWPRDVSLPRGAFPPRGPR---------------PVQSVVPEMEESGAQLLLEMLTFNPHKRIS-------AF-RALQHSYL--------------
---YEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLD-DDDE---GV--PSSALR-EICLLKEL-K---HKNIVRLHDVLHS------DKKLT--LVFEFCDQ---DLKKYFD---SCNG---DLDPEIVKSFLFQLLKGLGFCHS-RNVLHRDLKPQNLLIN-RNGEL---KLADFGLARAF---GIPVRCYSA---EVVTLWYRPPDVLFGAKL---YSTSIDMWSAGCIFAELANAGRPLFPGN----DVDDQLKRIFRLLG----TPTEEQWPSMTKLPDYKPYPMYPAT-------------TSLVNVVPKLNATGRDLLQNLLKCNPVQRIS-------AE-EALQHPYF--------------
--W-DLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMK-RAVD---CP--ENI-KK-EICINKML-N---HENVVKFYGHRRE------GNIQY--LFLEYCSGG--ELFDRIE----PDI---GMPEPDAQRFFHQLMAGVVYLHG-IGITHRDIKPENLLLD-ERDNL---KISDFGLATVFR-YNNRERLLNK---MCGTLPYVAPELLKRREF---HAEPVDVWSCGIVLTAMLA-GELPWDQP------SDSCQEYSDWKE----KKTYLNPWKKI--D-----------------------------------SAPLALLHKILVENPSARIT-------IP-DIKKDRW-Y-------------
---YTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQD-KRFK---NR---------ELQIMRKL-D---HCNIVRLRYFFYSSG--EKKDEVYLNLVLDYVPETVYRVA---RHYSRAKQ---TLPVIYVKLYMYQLFRSLAYIHS-FGICHRDIKPQNLLLDPDTAVL---KLCDFGSAKQ----LVRGEPNVS---YICSRYYRAPELIFGATD---YTSSIDVWSAGCVLAELLL-GQPIFPGD----SGVDQLVEIIKVLG----TPTREQIREMNPNYTEFKFPQIKAH-------------PWTKVFRPRTPPEAIALCSRLLEYTPTARLT-------PL-EACAHSFF--------------
---IKLVKRLGAGQFGEVWMGYYN-NSTKVAVKTLK-P-GTM----SV--QAFL-E-EANLMKTL-Q---HDKLVRLYAVVTR------EEPIY--IITEYMAKG--SLLDFLK-----SDEGGKVLLPKLIDFSAQIAEGMAYIER-KNYIHRDLRAANVLVS-ESLMC---KIADFGLARVI---E--DNEYTAREGAKFPIKWTAPEAIN-FGC---FTIKSDVWSFGILLYEIVTYGKIPYPGR----TNADVMTALSQGYR----MPRVENCP-----------------------------------------DELYDIMKMCWKEKAEERPT-------FD-YLQSV--L--------------
---YQNLKPIGSGAQGIVCAAYDAVLDRNVAIKKLSRP-FQNQ---TH--AKRAYR-ELVLMKCV-N---HKNIISLLNVFTPQKTLEEFQDVY--LVMELMDA---NLCQVIQ------M---ELDHERMSYLLYQMLCGIKHLHS-AGIIHRDLKPSNIVVK-SDCTL---KILDFGLART----AGTSFMMTP---YVVTRYYRAPEVIL-GMG---YKENVDIWSVGCIMGEMVR-HKILFPGR----DYIDQWNKVIEQLG----TPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRIS-------VD-DALQHPYI--------------
---YQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRP-FQSI---IH--AKRTYR-ELRLLKHM-K---HENVIGLLDVFTPARSLEEFNDVY--LVTHLMGA---DLNNIVK-----CQ---KLTDDHVQFLIYQILRGLKYIHS-ADIIHRDLKPSNLAVN-EDCEL---KILDFGLAR------HTDDEMTG---YVATRWYRAPEIMLNWMH---YNQTVDIWSVGCIMAELLT-GRTLFPGT----DHIDQLKLILRLVG----TPGAELLKKISSESARNYIQSLTQMP-----------KMNFANVFIGANPLAVDLLEKMLVLDSDKRIT-------AA-QALAHAYF--------------
KVT---SQVLGLGINGKVLQIFNKRTQEKFALKMLQDC-PKAR------------R-EVELHWRASQ---CPHIVRIVDVYENLY--AGRKCLL--IVMECLDGG--ELFSRIQD--RGDQ---AFTEREASEIMKSIGEAIQYLHS-INIAHRDVKPENLLYT-SKRPNAILKLTDFGFAKE----TTSHNSLTT---PCYTPYYVAPEVLG-PEK---YDKSCDMWSLGVIMYILLC-GYPPFYSN---HGLAISPGMKTRIRM----GQYEFPNPEWSEVS-----------------------------------EEVKMLIRNLLKTEPTQRMT-------IT-EFMNHPWI--------------
--F-NFLMVLGKGSFGKVMLADRKGTEELYAIKILKKD-VVIQ---DD--DVECTMVEKRVLALLDK---PPFLTQLHSCFQT------VDRLY--FVMEYVNGG--DLMYHIQ----QVG---KFKEPQAVFYAAEISIGLFFLHK-RGIIYRDLKLDNVMLD-SEGHI---KIADFGMCKEH---MMDGVTTRT---FCGTPDYIAPEIIA-YQP---YGKSVDWWAYGVLLYEMLA-GQPPFDGE-------DEDELFQSIME----HNVSYPKSLS---------------------------------------KEAVSVCKGLMTKHPAKRLG-------C----------GPEGERDVREHAFF
--F-NFLMVLGKGSFGKVMLSERKGTDELYAVKILKKD-VVIQ---DD--DVECTMVEKRVLALPGK---PPFLTQLHSCFQT------MDRLY--FVMEYVNGG--DLMYHIQ----QVG---RFKEPHAVFYAAEIAIGLFFLQS-KGIIYRDLKLDNVMLD-SEGHI---KIADFGMCKEN---IWDGVTTKT---FCGTPDYIAPEIIA-YQP---YGKSVDWWAFGVLLYEMLA-GQAPFEGE-------DEDELFQSIME----HNVAYPKSMS---------------------------------------KEAVAICKGLMTKHPGKRLG-------C----------GPEGERDIKEHAFF
-FI--FHKVLGKGSFGKVLLGELKGRGEYFAIKALKKD-VVLI---DD--DVECTMVEKRVLTLAAE---NPFLTHLICTFQT------KDHLF--FVMEFLNGG--DLMYHIQ----DKG---RFELYRATFYAAEIMCGLQFLHS-KGIIYRDLKLDNVLLD-RDGHI---KIADFGMCKEN---IFGESRAST---FCGTPDYIAPEILQ-GLK---YTFSVDWWSFGVLLYEMLI-GQSPFHGD-------DEDELFESIRV----DTPHYPRWIT---------------------------------------KESKDILEKLFEREPTKRLG------VTG-NIKIHPFF--------------
--F-NFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKD-VILQ---DD--DVDCTMTEKRILALARK---HPYLTQLYCCFQT------KDRLF--FVMEYVNGG--DLMFQIQ----RSR---KFDEPRSRFYAAEVTSALMFLHQ-HGVIYRDLKLDNILLD-AEGHC---KLADFGMCKEG---ILNGVTTTT---FCGTPDYIAPEILQ-ELE---YGPSVDWWALGVLMYEMMA-GQPPFEAD-------NEDDLFESILH----DDVLYPVWLS---------------------------------------KEAVSILKAFMTKNPHKRLGCVASQNGED-AIKQHP--FF------------
---FEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKD-VILQ---DD--DVECTMTEKRILSLARN---HPFLTQLFCCFQT------PDRLF--FVMEFVNGG--DLMFHIQ----KSR---RFDEARARFYAAEIISALMFLHD-KGIIYRDLKLDNVLLD-HEGHC---KLADFGMCKEG---ICNGVTTAT---FCGTPDYIAPEILQ-EML---YGPAVDWWAMGVLLYEMLC-GHAPFEAE-------NEDDLFEAILN----DEVVYPTWLH---------------------------------------EDATGILKSFMTKNPTMRLGSLT-QGGEH-AILRHPF-F-------------
-FI--LHKMLGKGSFGKVFLAEFKKTNQFFAIKALKKD-VVLM---DD--DVECTMVEKRVLSLAWE---HPFLTHMFCTFQT------KENLF--FVMEYLNGG--DLMYHIQ----SCH---KFDLSRATFYAAEIILGLQFLHS-KGIVYRDLKLDNILLD-KDGHI---KIADFGMCKEN---MLGDAKTNT---FCGTPDYIAPEILL-GQK---YNHSVDWWSFGVLLYEMLI-GQSPFHGQ-------DEEELFHSIRM----DNPFYPRWLE---------------------------------------KEAKDLLVKLFVREPEKRLG-------VRGDIRQHPLF--------------
--L-RLEVKLGQGCFGEVWMGTWN-GTTRVAIKTLK-P-GTM----SP--EAFL-Q-EAQVMKKL-R---HEKLVQLYAVVS-------EEPIY--IVTEYMSKG--SLLDFLK-----GETGKYLRLPQLVDMAAQIASGMAYVER-MNYVHRDLRAANILVG-ENLVC---KVADFGLARLI---E--DNEYTARQGAKFPIKWTAPEAAL-YGR---FTIKSDVWSFGILLTELTTKGRVPYPGM----VNREVLDQVERGYR----MPCPPECP-----------------------------------------ESLHDLMCQCWRKEPEERPT-------FE-YLQAF--L--------------
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