|
|
Multiple sequence alignment of family kinases
Conserved residues:
Group Kinase
AGC RPS6KA1 203
TK ABL1 261
TK ALK 1116
CMGC CDK2 4
CMGC CDK9 2.7.11.23
CAMK CHEK2 220
TK EGFR 712
CMGC GSK3B 56
TK HCK 241
TK IGF1R 999
TK INSR 1023
CAMK MAPKAPK2 64
Other NEK2 8
AGC PDPK1 82
CAMK PIM1 38
AGC PRKCI 245
TK RET 724
AGC SGK1 98
TK SRC 270
Other TTK 525
---FELLKVLGQGSFGKVFLVRKVTRP-----DSGHLYAMKVLKKATLKVRD--RV-RTKM------ERDIL-ADV-N-HPF--VVKLHYAFQT--------EGKLY--LILDFLRGG--DLFTRLS----KEV-------------------------MFTEEDVKFYLAELALGLDHLHSL-----GIIYRDLKPENILLD-E---EGHI---KLTDFGLSKEA-----IDHEKKAYS---FCGTVEYMAPEVVN-----------RQGHSHSADWWSYGVLMFEMLT-GSLPFQGK---------DRKETMTLILKAKLGMPQFLS-----------------------------TEAQSLLRALFKRNPANRLGSG-PDGAEEIKRHV--FY---------
---ITMKHKLGGGQYGEV---YEGVWK-----KYSLTVAVKTLK-EDTM-EV--EE-FL-K------EAAVM-KEI-K-HPN--LVQLLGVCTR--------EPPFY--IITEFMTYG--NLLDYLR-----EC----------------------NRQEVNAVVLLYMATQISSAMEYLEKK-----NFIHRDLAARNCLVG-E---NHLV---KVADFGLSRLM-----T--GDTYTAHAGAKFPIKWTAPESLA-----------YNKFSIKSDVWAFGVLLWEIATYGMSPYPGI------DLSQVYELLEKDYRMERPEGCP-------------------------------EKVYELMRACWQWNPSDRPS------FAEIHQA--F-----------
--I-TLIRGLGHGAFGEV---YEGQVSGMPNDPSPLQVAVKTLP-EVCS-EQDELD-FL-M------EALII-SKF-N-HQN--IVRCIGVSLQ--------SLPRF--ILLELMAGG--DLKSFLR-----ETRP-----------------RPSQPSSLAMLDLLHVARDIACGCQYLEEN-----HFIHRDIAARNCLLT-CPGPGRVA---KIGDFGMARDI--Y--R--ASYYRKGGCAMLPVKWMPPEAFM-----------EGIFTSKTDTWSFGVLLWEIFSLGYMPYPSK------SNQEVLEFVTSGGRMDPPKNCP-------------------------------GPVYRIMTQCWQHQPEDRPN------FAIILER--I-----------
---FQKVEKIGEGTYGVV---YKARNK-----LTGEVVALKKIRLDTETEGV--PS-TAIR------EISLL-KEL-N-HPN--IVKLLDVIHT--------ENKLY--LVFEFLHQ---DLKKFMDA--SALT-------------------------GIPLPLIKSYLFQLLQGLAFCHSH-----RVLHRDLKPQNLLIN-T---EGAI---KLADFGLARAF-----GVPVRTYTH---EVVTLWYRAPEILL----------GCKYYSTAVDIWSLGCIFAEMVT-RRALFPGD------SEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWAR---QDFSKVVPPLDEDGRSLLSQMLHYDPNKRIS------AKAALAHPFF-----------
---YEKLAKIGQGTFGEV---FKARHR-----KTGQKVALKKVLMENEKEGF--PI-TALR------EIKIL-QLL-K-HEN--VVNLIEICRTKASPYNRCKGSIY--LVFDFCEH---DLAGLLS---NVLV-------------------------KFTLSEIKRVMQMLLNGLYYIHRN-----KILHRDMKAANVLIT-R---DGVL---KLADFGLARAFSL-AKNSQPNRYTN---RVVTLWYRPPELLL----------GERDYGPPIDLWGAGCIMAEMWT-RSPIMQGN------TEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRID------SDDALNHDFF-----------
-YI--MSKTLGSGACGEV---KLAFER-----KTCKKVAIKIISKRKFAIGS--AR-EADPALNVETEIEIL-KKL-N-HPC--IIKIKNFF-D--------AEDYY--IVLELMEGG--ELFDKVV----GNK-------------------------RLKEATCKLYFYQMLLAVQYLHEN-----GIIHRDLKPENVLLS-S---QEEDCLIKITDFGHSKI------LGETSLMRT---LCGTPTYLAPEVLVS--------VGTAGYNRAVDCWSLGVILFICLS-GYPPFSEH--------RTQVSLKDQITSGKYNFIPEVWAEVS-------------------------EKALDLVKKLLVVDPKARFT------TEEALRHPWL-----------
--F-KKIKVLGSGAFGTV---YKGLWIP-EGEKVKIPVAIKELR-EATS-PKANKE-IL-D------EAYVM-ASV-D-NPH--VCRLLGICL---------TSTVQ--LITQLMPFG--CLLDYVR-----EH-----------------------KDNIGSQYLLNWCVQIAKGMNYLEDR-----RLVHRDLAARNVLVK-T---PQHV---KITDFGLAKLL--G--A--EEKEYHAEGGKVPIKWMALESIL-----------HRIYTHQSDVWSYGVTVWELMTFGSKPYDGI------PASEISSILEKGERLPQPPICT-------------------------------IDVYMIMVKCWMIDADSRPK------FRELI----IEF---------
---YTDTKVIGNGSFGVV---YQAKLC-----DSGELVAIKKVLQDKRFKNR---------------ELQIM-RKL-D-HCN--IVRLRYFFYSSG----EKKDEVYLNLVLDYVPETVYRVA---RHYSRAKQ-------------------------TLPVIYVKLYMYQLFRSLAYIHSF-----GICHRDIKPQNLLLDPD---TAVL---KLCDFGSAKQ------LVRGEPNVS---YICSRYYRAPELIF----------GATDYTSSIDVWSAGCVLAELLL-GQPIFPGD------SGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAH---PWTKVFRPRTPPEAIALCSRLLEYTPTARLT------PLEACAHSFF-----------
---LKLEKKLGAGQFGEV---WMATYN------KHTKVAVKTMK-PGSM-SV--EA-FL-A------EANVM-KTL-Q-HDK--LVKLHAVVT---------KEPIY--IITEFMAKG--SLLDFLK-----SD----------------------EGSKQPLPKLIDFSAQIAEGMAFIEQR-----NYIHRDLRAANILVS-A---SLVC---KIADFGLARVI-----E--DNEYTAREGAKFPIKWTAPEAIN-----------FGSFTIKSDVWSFGILLMEIVTYGRIPYPGM------SNPEVIRALERGYRMPRPENCP-------------------------------EELYNIMMRCWKNRPEERPT------FEYIQSV--L-----------
---ITMSRELGQGSFGMV---YEGVAKGVVKDEPETRVAIKTVN-EAAS-MRERIE-FL-N------EASVM-KEF-N-CHH--VVRLLGVVSQ--------GQPTL--VIMELMTRG--DLKSYLR-----SLRPEM--------------ENNPVLAPPSLSKMIQMAGEIADGMAYLNAN-----KFVHRDLAARNCMVA-E---DFTV---KIGDFGMTRDI--Y--E--TDYYRKGGKGLLPVRWMSPESLK-----------DGVFTTYSDVWSFGVVLWEIATLAEQPYQGL------SNEQVLRFVMEGGLLDKPDNCP-------------------------------DMLFELMRMCWQYNPKMRPS------FLEIISS--I-----------
---ITLLRELGQGSFGMV---YEGNARDIIKGEAETRVAVKTVN-ESAS-LRERIE-FL-N------EASVM-KGF-T-CHH--VVRLLGVVSK--------GQPTL--VVMELMAHG--DLKSYLR-----SLRPEA--------------ENNPGRPPPTLQEMIQMAAEIADGMAYLNAK-----KFVHRDLAARNCMVA-H---DFTV---KIGDFGMTRDI--Y--E--TDYYRKGGKGLLPVRWMAPESLK-----------DGVFTTSSDMWSFGVVLWEITSLAEQPYQGL------SNEQVLKFVMDGGYLDQPDNCP-------------------------------ERVTDLMRMCWQFNPKMRPT------FLEIVNLL-------------
KVT---SQVLGLGINGKV---LQIFNK-----RTQEKFALKMLQDCPKAR----------R------EVELH-WRASQ-CPH--IVRIVDVYENLY----AGRKCLL--IVMECLDGG--ELFSRIQD--RGDQ-------------------------AFTEREASEIMKSIGEAIQYLHSI-----NIAHRDVKPENLLYT-S---KRPNAILKLTDFGFAKE------TTSHNSLTT---PCYTPYYVAPEVLG-----------PEKYDKSCDMWSLGVIMYILLC-GYPPFYSN-----HGLAISPGMKTRIRMGQYEFPNPEWSEVS-------------------------EEVKMLIRNLLKTEPTQRMT------ITEFMNHPWI-----------
---YEVLYTIGTGSYGRC---QKIRRK-----SDGKILVWKELDYGSMTEAE--KQ-MLVS------EVNLL-REL-K-HPN--IVRYYDRIIDR------TNTTLY--IVMEYCEGG--DLASVITKGTKERQ-------------------------YLDEEFVLRVMTQLTLALKECHRRSDGGHTVLHRDLKPANVFLD-G---KQNV---KLGDFGLARIL-----NHDTSFAKT---FVGTPYYMSPEQMN-----------RMSYNEKSDIWSLGCLLYELCA-LMPPFTAF---------SQKELAGKIREGKFRRIPYRY---S-------------------------DELNEIITRMLNLKDYHRPS------VEEILENPL-I----------
--F-KFGKILGEGSFSTV---VLAREL-----ATSREYAIKILEKRHIIKEN--KVPYVTR------ERDVM-SRL-D-HPF--FVKLYFTFQD--------DEKLY--FGLSYAKNG--ELLKYIR----KIG-------------------------SFDETCTRFYTAEIVSALEYLHGK-----GIIHRDLKPENILLN-E---DMHI---QITDFGTAKVLS---PESKQARANS---FVGTAQYVSPELLT-----------EKSACKSSDLWALGCIIYQLVA-GLPPFRAG---------NEYLIFQKIIKLEYDFPEKFF-----------------------------PKARDLVEKLLVLDATKRLG------CEEM------EGYGPLKAHPF
---YQVGPLLGSGGFGSV---YSGIRV-----SDNLPVAIKHVEKDRISDWG--EL-PNGTRV--PMEVVLL-KKV-S-SGFSGVIRLLDWFER--------PDSFV--LILERPEPV-QDLFDFIT----ERG-------------------------ALQEELARSFFWQVLEAVRHCHNC-----GVLHRDIKDENILIDLN---RGEL---KLIDFGSGA-------LLKDTVYTD---FDGTRVYSPPEWIR----------YHRYHGRSAAVWSLGILLYDMVC-GDIPFEHD---------------EEIIRGQVFFRQRVS-----------------------------SECQHLIRWCLALRPSDRPT------FEEIQNHPWM-----------
--F-DLLRVIGRGSYAKV---LLVRLK-----KTDRIYAMKVVKKELVNDDE--DI-DWVQ-----TEKHVF-EQASN-HPF--LVGLHSCFQT--------ESRLF--FVIEYVNGG--DLMFHMQ----RQR-------------------------KLPEEHARFYSAEISLALNYLHER-----GIIYRDLKLDNVLLD-S---EGHI---KLTDYGMCKEG-----LRPGDTTST---FCGTPNYIAPEILR-----------GEDYGFSVDWWALGVLMFEMMA-GRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLS-----------------------------VKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPF-F----------
--L-VLGKTLGEGEFGKV---VKATAFHLKGRAGYTTVAVKMLK-ENAS-PSELRD-LL-S------EFNVL-KQV-N-HPH--VIKLYGACSQ--------DGPLL--LIVEYAKYG--SLRGFLR-----ESRKVGPGYLGSGGSRNSSSLDHPDERALTMGDLISFAWQISQGMQYLAEM-----KLVHRDLAARNILVA-E---GRKM---KISDFGLSRDV--Y--E--EDSYVKRSQGRIPVKWMAIESLF-----------DHIYTTQSDVWSFGVLLWEIVTLGGNPYPGI------PPERLFNLLKTGHRMERPDNCS-------------------------------EEMYRLMLQCWKQEPDKRPV------FADISKDL-------------
---FHFLKVIGKGSFGKV---LLARHK-----AEEVFYAVKVLQKKAILKKK--EE-KHIM-----SERNVLLKNV-K-HPF--LVGLHFSFQT--------ADKLY--FVLDYINGG--ELFYHLQ----RER-------------------------CFLEPRARFYAAEIASALGYLHSL-----NIVYRDLKPENILLD-S---QGHI---VLTDFGLCKEN-----IEHNSTTST---FCGTPEYLAPEVLH-----------KQPYDRTVDWWCLGAVLYEMLY-GLPPFYSR---------NTAEMYDNILNKPLQLKPNIT-----------------------------NSARHLLEGLLQKDRTKRLGAK--DDFMEIKSHV--FF---------
--L-RLEVKLGQGCFGEV---WMGTWN------GTTRVAIKTLK-PGTM-SP--EA-FL-Q------EAQVM-KKL-R-HEK--LVQLYAVVS---------EEPIY--IVTEYMSKG--SLLDFLK-----GE----------------------TGKYLRLPQLVDMAAQIASGMAYVERM-----NYVHRDLRAANILVG-E---NLVC---KVADFGLARLI-----E--DNEYTARQGAKFPIKWTAPEAAL-----------YGRFTIKSDVWSFGILLTELTTKGRVPYPGM------VNREVLDQVERGYRMPCPPECP-------------------------------ESLHDLMCQCWRKEPEERPT------FEYLQAF--L-----------
---YSILKQIGSGGSSKV---FQVLNE-----KKQ-IYAIKYVNLEEADNQT--LD-SYRN------EIAYL-NKLQQHSDK--IIRLYDYEIT--------DQYIY--MVMECGNI---DLNSWLK----KKK-------------------------SIDPWERKSYWKNMLEAVHTIHQH-----GIVHSDLKPANFLIV-----DGML---KLIDFGIANQMQ---PDTTSVVKDS---QVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTY-GKTPFQQI--------INQISKLHAIIDPNHEIEFPDIPE---------------------------KDLQDVLKCCLKRDPKQRIS------IPELLAHPYV-----------
|